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Liquid Chromatography of Non-Chromophores by Indirectly Photometric Detection (간접 분광광도 측정에 의한 비발색단의 액체크로마토그래피)

  • Sam-Woo Kang;Hae-Beom Oh;Young-Ja Moon
    • Journal of the Korean Chemical Society
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    • v.31 no.2
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    • pp.184-189
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    • 1987
  • Brilliant green was examined as a detection reagent in the separation of some aliphatic alcohols, ketones and esters by the reverse-phase liquid chromatography. These samples not possessing chromophore could be indirectly detected by photometric detector with the assistance of brilliant green in visible range (625nm). Some mixtures of samples were also able to separated and detected with good base line resolution and comparatively high sensitivity.

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A Study on the Synthesis of Magenta Coupler for Color Photograph and Color Development (천연색 사진용 Magenta 발색제의 합성과 발색현상에 관한 연구)

  • Kim, Yeoung-Chan;Lee, Byung-Dae
    • Journal of the Korean Applied Science and Technology
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    • v.23 no.1
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    • pp.92-97
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    • 2006
  • In this paper, magenta coupler was prepared by the reaction of 1-(2,4,6-Trichlorophenyl)-3-(5-amino-2-chloroanilino)-5-pyrazolone with 2,4-di-tert-pentyl-phenoxy butanoyl chloride in the presence of pyridine. The product was identified by using various analytical tools such as melting point, elemental analyzer, IR spectrophotometer, UV-Vis spectrophotometer and GC-mass spectrometer. The reaction of magenta coupler with CD-3 (color development agent) was shown magenta color.

A Studies on Indirect Photometric Detection of Aliphatic Compounds by High-Performance Liquid Chromatography (고성능 액체 크로마토그래피에 의한 지방족 화합물의 간접 분광광도 측정에 관한 연구)

  • Sam-Woo Kang;Jeon-Tag Kang
    • Journal of the Korean Chemical Society
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    • v.33 no.4
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    • pp.406-412
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    • 1989
  • Indirect photometric detection of aliphatic compounds such as alcohols, ketones and esters was investigated in high-performance liquid chromatography, These samples possessing nonchromophore or weak chromophore could be indirectly detected by a UV detector with addition of UV absorbing, p-chlorophenol or p-nitrophenol in mobile phase as detection reagent, and submicrogram detection limits were achieved. Some mixtures of samples were also able to be separated and quantitated with good resolution and comparatively high sensitivity under optimum conditions.

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TLC 이용한 젖산 분리와 정량분석 개발을 위한 연구

  • Jo, Gap-Su;Choe, Mi-Hwa;Ryu, Hwa-Won;Kim, Geun-A;Yu, Seon-Gyun;Kim, Do-Man
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.165-168
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    • 2002
  • TLC(Thin Layer Chromatography) 는 제약산업이나 생화학 연구 그리고 여러 산업 현장에서 널리 이용되는 화학 분석 방법이다 . 본 연구에서는 적은 비용으로도 많은 양의 시료를 신속하게 분리할 수 있는 TLC를 이용하여 유기산인 젖산 (Lactic acid) 을 분리는 전개방법을 개발하였다 . 전개용매는 2 가지 용매 (1) nitroethane nitromethane : ethanol: water: I-propanol = 1 : 2 : 3 : 4 : 5 (v/v/v/v/v), (2) diisopropyl ether. formic acid. water = 90 : 7 : 3 (v/v/v) 를 사용하였다 . 발색시약은 A : bromocresol purple reagent I, B . bromocresol purple reagent II, C : bromocresol green-bromophenol blue-potassium permanganate reagent 를 사용하여 분석하는 방법을 개발하였다 $^6)$. 젖산의 분리는 silica gel TLC plate를 이용하는 경우 , 용매 (1) 에 발색시약 B를 사용했을 때 , 분리 확인이 가장 좋았다.

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Characteristics of Color Development of the Black Concrete depending on CASB added Superplasticizer Dosages (CASB-SP 혼입율 변화에 따른 블랙콘크리트의 발색 특성)

  • Kim, Kyoung-Hoon;Hong, Seak-Min;Yoo, Seung-Yeup;Oh, Chi-hyun;Han, Min-Cheol;Han, Cheon-Goo
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2012.05a
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    • pp.73-75
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    • 2012
  • In this study, basic physical properties and color development of the black concrete depending on CASB-SP dosages are investigated. According to results, It showed that the slump and air contents of the concrete containing CASB-SP meet all requirements of concrete except when CASB-SP 5.0 % is used. It also showed that the more the CASB-SP is used, the higher the compressive strength becomes. On the other hand, It is found that if the CASB-SP 5.0 % is used, the compressive strength was reduced as the more CASB-SP 5 % is used. In terms of color, it was found out that the more CASB-SP is used, the darker the black becomes.

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