• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1997년도 춘계학술대회
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• pp.110-110
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• 1997

새로운 발모제 개발에 관한 연구의 일환으로 현재 발모제로 쓰여지고 있는 Eugenol에 구충제로 쓰여지고 있는 cinnamic acid를 반응시켜 4-allyl-2-methoxyphenyl cinnamoyl ester(이하 Eucin이라 칭함)을 만들어 물성, 정량법 및 육모작용 등에 관하여 검토하였다. Eucin은 분자식 $C_{19}$ H$_{18}$ O$_3$ (mw : 294), mp 88-90$^{0}$ C인 백색분말로서 클로로포름, 디클로로메탄 등에 녹고, 메탄올에는 약간 녹으며 물 및 핵산에는 불용이었다. Eucin 메탄올용액의 EtOAc:n-Hexane (1:10) 에서의 Rf=0.24였다. 정량법은 흡광광도법 즉 1 - 5 $\times$ $10^{-5}$M Eucin 표준액에 대하여 최대흡광파장 282.4nm에서 각각 흡광도를 측정하여 구한 검량선식은 Y = 2.1709 $\times$ $10^4$X + 0.07725 (상관계수:997)이며, 또한 HPLC법으로 작성한 검량선식은 $Y_{area}$=3.1578 $\times$ $10^{9}$ X - 1.7958 $\times$ $10^3$(상관계수 : 0.9996)로서 양호한 직선성을 나타내었다. 그리고 육모시험결과는 미녹시딜에 비해 다소 떨어지는 모 복원효과를 나타내었으나, 차후 이 물질의 용해도 등을 개선함으로서 더 정화한 결과를 얻을 수 있으리라 사료된다.다.

• 대한화장품학회지
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• 제36권3호
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• pp.215-219
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• 2010

본 연구에서는 발모의 성장결여로부터 모내기의 천연성분에 의한 침투력, 침투면적, 모발성장속도, 모발의 수 및 표면에 미치는 영향에 관하여 조사를 수행하였다. 제모를 통한 모발의 성장 속도와 모내기의 피부 침투력, 약물도달 부위를 CLSM710 공초점 현미경으로 관찰하였는데, 고삼, 단삼 및 인삼의 혼합물인 모내기를 $10\;{\mu}g/mL$ 농도로 처리한 그룹에서는 정상군과 대조군과 비교시 $33{\pm}4$과 $60{\pm}6$ (AFI: 인위적 형광강도)인데 반해, 약 $6782{\pm}67$ AFI (인위적 형광강도)으로 약 205배와 113배의 침투능력을 나타내었고 면적은 정상군과 대조군은 각각 약 $22{\pm}3$와 $44{\pm}6$ AFI인데 반해 모내기군은 약 $5602{\pm}45$ AFI였고 각각 255배와 127배 정도 침투면적의 확대되었음을 관찰하였다. 또한 confocal LSM700으로 관찰한 결과 대조군은 진피층에 존재하는 collagen IV에서 아무런 녹색형광이 없는 반면 모내기군은 collagen IV이 함유한 진피층에서 강한 녹색형광이 관찰하였는데, 이는 모내기 주성분이 피부 진피층까지 도달한 것이라 사료되고 남자 15명과 여자 5명 중 20대 4명(20 %), 30대 10명(50 %), 그리고 40대 이상 6명(30 %)의 내원환자의 탈모의 털 직경과 표면, 탈모의 수, 그리고 발모의 수를 조사하여 본 결과 평균 약 75 % 개선효과를 나타내므로 모내기의 천연성분이 발모인자의 활성화를 촉진하여 모낭 성장을 촉진시키는 기능을 유도하리라 사료된다.

• 대한두개안면성형외과학회지
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• 제14권1호
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• pp.1-10
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• 2013

Background: Recently, substances from seaweeds have been widely used in hair growth solutions, and have been proven to be effective. Seaweeds have been documented to possess hair growth activity; however, no report on the effect of seaweed on hair regeneration has been issued to date. In this study, we investigated which exact substance of hair tonic made by JW-bio and our institute shows effects on hair growth by studying the mechanisms of candidate substances. Methods: The study was conducted to investigate the hair restoring effect of domestic natural substances; we categorized the candidate substances as seaweed, cereal, and herbal medicine. Five experimental groups were included in the study as follows: a saline group, a 50% ethanol group, seaweed group, a cereal group, and a herbal medicine group. Results: Three extracts (seaweed, cereal, and herbal medicine) were administered to C57BL/6 mice for two weeks after depilation. Depilated areas were found to be completely covered with fully grown hair, and the hair re-growth score was highest in the seaweed group. Using a hair analysis system, hair characteristics were measured in all groups on days 10 and 14 after depilation. The width and length of hair follicles were largest in the seaweed group. Groups treated with seaweed showed significantly increased gene expression of insulin-like growth factor-1. Groups treated with all the three extracts showed decreased expression of transforming growth factor-${\beta}1$. Conclusion: Findings from our study suggest that seaweeds possess hair-growth effects and may be useful for the treatment of alopecia in the future.

• 한방안이비인후피부과학회지
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• 제29권3호
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• pp.259-273
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• 2016

Objectives: Objectives of this research is to test hair growth effect of the hair scalpdetox five step program.Methods : Data for this research is gathered from participants who are suffering from hair loss and have completed scalpdetox five step program at a esthetic by receiving treatments twenty times constantly with once or twice a week during three months from March 2015 to April 2016. The hair scalpdetox program consists of five steps : the first step is shampooing, the second is scalp massage with aroma oils, the third is vertical meridian scrapping massage, the fourth is applying scalp and hair pack made with natural herbs, and the last is scalp massage with an indirect high frequency massaging device.Results: Subjective evaluations from the participants show that overall hair scalp conditions are meaningfully improved. Participants evaluated that tested approach helps hair growth by increasing the number of hair (p<.01) and helps to make their hair thicker (p<.01). Visual observations with magnification by 60 times and 200 times show improvement in hair scalp condition and clean opening of pores. Photographs of hair loss parts of each participant also show improvement in hair growth.Conclusions: From the evaluation of the participants for the test program and from visual observations, we conclude that suggested hair scalpdetox five step program has effect on improving scalp, hair condition, and hair growth.

• 한방안이비인후피부과학회지
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• 제29권3호
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• pp.74-94
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• 2016

Objectives : YangHyulEum Gami-Bang(YHEG) is a hair care extracts which is composed of fourteen plant extracts used in oriental medicine. The purpose of this study is to investigate the effect of YangHyulEum Gami-Bang(YHEG) on the alopecia and hair growth.Methods & Results : The herbal extracts from YangHyulEum Gami-Bang(YHEG) was tested using in vivo and in vitro test models. 1. The YHEG extracts showed effect on the DNA proliferation of the hair dermal papilla cells measured by [3H]thymidine incorporation. 2. YHEG showed promoting on the expression of growth factors such as IGF-1, KGF-1 and inhibiting on the expression of inhibitory hair growth factor such as TGF-β1, BMP-2 estimated by qPCR. 3. The YHEG extracts showed effect on the activation of β-catenin in the dermal papilla cells. 4. YHEG showed inhibitory effects of NO synthesis at 0.2% concentrations. 5. YHEG showed effects in the expression of IL-1β, TNF-α, IL-6, COX-2 and iNOS gene in the LPS stimulated RAW 264.7 cells. 6. The hair growth index of the YHEG extracts ranked at over 2 when compared to control group which was ranked at 0. 7. The hair follicle number, length and size of the experimental group were remarkably higher than the control group in the histological observation.Conclusions : These results suggest that YangHyulEum Gami-Bang(YHEG) has hair growth promoting activity and it can be used as a potent treatment agent for preventing hair loss and stimulating hair growth for treatment of alopecia.

• 동의생리병리학회지
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• 제20권2호
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• pp.428-435
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• 2006

Cortex Mori radicis has been used as a components of antidiabetics, antiasthma and diuresis in Oriental Medicine. This experiment examined the effect of an extracts, obtained from the acetone(CM-A) and aqueous(CM-W) extracts of Cortex Mori radicis, on hair growing activity of the C57BL/6N mice after topical application to skin. We investigated the number of hair follicle and mast cells, and changes of subpopulation of splenocytes and thymocytes in skin for 16 day. The results were as follows : The Hair growing effect in experimental groups was more increased in 85%(CM-A) and 90%(CM-W) than control group(10%) in hair depilated area. The number of hair follicle in experimental groups(CM-A and CM-W) was more increased than control group. Splenic B/T lymphocytes of CM-A group were decreased compare to control group. CD4/CD8 positive TH cells in splenic T lymphocytes of CM-W group were increased compare to control group. These results suggest that CM-A and CM-W may be used in treatment of alopecia areata.

• 생명과학회지
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• 제32권3호
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• pp.196-201
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• 2022

곤충과 같은 새로운 단백질 공급원은 인간의 영양을 위해 제안됩니다. 단백질 가용성은 곤충 특성에 영향을 받을 수 있습니다. 발효를 통해 독특한 특성을 지닌 다양한 곤충 기반 성분 및 식품을 얻을 수 있습니다. 그러나 발효된 식용 곤충이 단백질 가용성에 미치는 영향은 알려져 있지 않습니다. 발효 쌍별 귀뚜라미 분말은 주로 단백질로 구성됩니다. 이전에는 귀뚜라미 분말의 경구 투여가 남성형 탈모증에서 모발 성장을 개선하는 것으로 보고 되었습니다. 본 연구의 목적은 동물모델에서 쌍별 귀뚜라미 발효분말의 모발 촉진 활성을 평가하는 것입니다. 수컷 C57BL/6 마우스(25-30 g)를 사용했습니다. 실험을 위해서 마우스의 복부 털(2×2.5 cm)을 부드럽게 제거했습니다. 그룹은 다음과 같이 처리되었습니다. 정상 급이군(쌍별귀뚜라미 분말 없음), 쌍별귀뚜라미 분말 급이군 및 발효 쌍별 귀뚜라미 분말 급이군으로 실험군은 구성 되었습니다. 음식은 11주 동안 공급하였습니다. 관찰을 수행하고 대조군 및 실험군의 결과를 비교했습니다. 대조군과 비교 하였을 때, 발효 쌍별 귀뚜라미 분말의 적용은 유의하게(p<0.01) 모발 성장을 촉진했습니다. C57BL/6 실험의 결과는 쌍별 귀뚜라미 발효 분말은 7주 후 모발 성장이 급격히 증가 되었음을 확인시켜줍니다. 급이 7주 후, 성장기의 모발 비율을 정상 급이군과 쌍별 귀뚜라미 분말 급이군에서 각각 비교한 결과, 발효 쌍별 귀뚜라미 급이군에서 약 125% 및 약 120% 증가하였습니다. 결론적으로 쌍별 귀뚜라미 발효 분말의 적용은 발모를 촉진하기 때문에 탈모증 치료의 유망한 대안으로 볼 수 있습니다. 또한 구성을 고려할 때 아미노산과 같은 미량 원소가 이러한 효과에 기여했을 수 있습니다.

• 대한화장품학회지
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• 제44권3호
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• pp.349-362
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• 2018

한련초는 열대 및 아열대 지방에 서식하는 국화과 한련초속에 속하는 한해살이풀로 간 손상에 대한 보호효과, 살무사 독에 대한 해독효과, 항산화효과, 발모효과, 상처치유효과 등의 효능들이 확인된 바 있다. 더 나아가 본 연구에서는 한련초의 항산화 및 항노화효과를 확인하여 피부보호 소재로써의 가능성을 검토하고자 하였다. 이를 위해 한련초를 50% 에탄올로 추출한 추출물과 이를 다시 에틸아세테이트로 분획하여 얻은 분획물을 실험에 사용하였다. 항산화 지표인 $FSC_{50}$과 $OSC_{50}$을 측정한 결과, 에틸아세테이트 분획물의 $OSC_{50}$은 항산화제로 잘 알려진 L-ascorbic acid 보다 2.7배 이상 뛰어난 것으로 나타났다. 이와 더불어 시료의 세포 내 활성산소종 소거능과 $H_2O_2$로 유도된 세포손상에 대한 보호효과가 확인되었으며, 특히 $^1O_2$으로 유도된 사람 적혈구 광용혈에 대한 지연효과의 평가에서 $64{\mu}g/mL$의 에틸아세테이트 분획물이 적혈구 광용혈의 소요시간을 6배 이상 지연시켜 한련초의 우수한 항산화능을 반영해주었다. 한련초의 항노화 효과를 검증하기 위해 Hs68에서 추출한 엘라스타제에 대한 저해활성을 평가한 결과, $16{\mu}g/mL$의 두 시료 모두 엘라스타제의 활성을 각각 6.8%와 14.0% 억제한 것을 확인하였다. 마지막으로 한련초가 식품 또는 화장품 등의 소재로 사용됨에 있어 화학적 방부제의 역할을 대체할 수 있을 가능성을 확인하기 위한 항균실험의 결과, 에틸아세테이트 분획물이 그람 양성 균주인 Staphylococcus aureus에 대해 mehyl paraben과 동등하거나 더 뛰어난 항균활성을 가진 것을 확인하였다. 이상의 실험결과들을 통해 한련초는 피부보호 소재 또는 천연방부제로서의 가능성을 가진 천연소재라 생각되며, 식품 또는 화장품 소재로서의 이용이 기대된다.

• 동의생리병리학회지
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• 제21권1호
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• pp.136-144
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• 2007

Biotae Orientalis has been widely used for treatment of relaxion of smooth muscle, gastrointestinal hemorrhage and alopecia in Oriental Medicine. This experiment examined the effect of an extracts, obtained from the acetone and MeOH extracts of dried or fresh Biotae Orientalis, on hair growing activity of the C57BL/6N mice after topical application to skin. First, We examined on hair growth activity of extracts of Biotae Orientalis compare to control and 1% minoxidil groups. Second, We investigated on the number of hair follicle and mast cells after topical application of extracts of the Biotae Orientalis to skin for 16 day. Third, We investigated immunoreactive density of vascular endothelial growth factor(VEGF), protein kinase C-${\alpha}$(PKC-${\alpha}$) and stem(mast) cell factor(SCF) in skin of C57BL/6N mice by immunohistochemical methods. I fourth investigated changes of subpopulation of splenocytes and thymocytes in C57BL/6N mice for 16day using laser flow cytometry. The results were as follows : Hair growing effect of acetone and MeOH extracts of dried and fresh Biotae Orientalis was observed in 70%, 90% and 60% in hair removed skin area in 16 day respectively. Immunoreactive density of VEGF and PKC-${\alpha}$ in skin of experimental groups was weakly stained compare to control group in 10 day. Immunoreactive density of stem cell factor in skin of experimental group was heavily stained compare to control group in 10 day. Splenic TH/TC Iymphocytes of lived MeOH extracts group significantly increased compare to control group. TH cells in thymic T lymphocytes were increased compare to control group. These experiment suggest that acetone and MeOH extracts of Biotae Orientalis may be used for topical treatment of alopecia areata.

• 동의생리병리학회지
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• 제23권5호
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• pp.1116-1124
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• 2009

To determine whether topical application of trimix extracts of Mylabris phalerata Pall., Arisaematis Rhizoma and Pinelliae Rhizoma Ternata lead to affects on the hair growth activity in C57BL/6N mice. To examine the hair growth activity of the extracts of Mylabris phalerata Pall., Arisaematis Rhizoma and Pinelliae Rhizoma Ternata gross, microscopic, and immunohistochemical method were performed. In order to examine the mRNA expression of hair growth related substance, RT-PCR method was performed. Experimental group I on day 14, The most extensive hair growth activity was observed in whole skin area of all the mice whose hair had been clipped. Brdu immunoreactive cells of all the experimental groups were more heavily stained in epidermis, bulge, outer root sheath, inner root sheath, subcutaneous tissue, hair bulb and cutaneous trunci muscles than that of control group on day 12 of hair growing cycle in C57BL/6N mice. VEGF immunoreactive density of all the experimental groups was more heavily stained in epidermis, bulge and cutaneous trunci muscles than that of control group on day 12. FGF and c-kit immunoreactive cells of all the experimental groups were heavily stained in epidermis, outer root sheath, inner root sheath and cutaneous trunci muscles on day 12. PKC-$\alpha$ immunoreactive density of all the experimental groups was mildly stained in epidermis and cutaneous trunci muscles than that of control group on day 12. On day 12, the expression of bFGF (138%, 119%, 120%), VEGF (146%, 144%, 133%), IGF-1 (165%, 141%, 119%) and PLI (121%, 116%, 123&) in each experimental groups was more increased than that of control group. On day 16, The expression of IGF-1 (126%, 149%, 151%) in all the experimental group was more increased than that of control group (100%). The expression of bFGF (92%, 94%) and VEGF (101%, 97%), PL1 (102%, 109%) in all the experimental group was more decreased than that of experimental group I, II on day 12. But the expression of bFGF (109%) and VEGF (127%), and PL1 (105%) in each experimental group III was more increased than that of control group (100%). These experiments suggest that trimix extracts of Mylabris phalerata pall., Arisaematis Rhizoma and Pinelliae Rhizoma Ternata may stimulate the topical hair growth activity and its experimental group I can be useful for treatment of alopecia areata.