• Applied Biological Chemistry
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• v.36 no.1
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• pp.7-10
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• 1993

ELISA method was applied for the screening of zearalenone producing strains. The developed ELISA was as follow: $125\;{\mu}l$ of diluted solution (1 : 500) of antibody was added to each microtiter well and incubated overnight at $40^{\circ}C$. For direct competitive ELISA, samples and zearalenone-peroxidase conjugate were mixed in a 1 : 1 ratio, and a $100\;{\mu}l$ of aliquot was then added to antisera-coated wells. Plates were incubated for 30 minutes at $37^{\circ}C$, and wells washed 6 times, and $100\;{\mu}l$ of ABTS substrates was added. Plates were incubated for antother 15 minutes at $37^{\circ}C$, and $100\;{\mu}l$ of stopping reagent was added to the wells and absorbance was recorded at 410nm on ELISA Reader. Among 19 strains showed zearalenone-producing ability by ELISA, 3 strains (R-5, C-46, S-134) produced more than 50 ng/ml of zearalenone.

• The Journal of the Korea Contents Association
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• v.7 no.1
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• pp.40-47
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• 2007

In the protein macro array image, it is important to find out the feature of the each protein chip. A decision error by the personal sense of sight occurred from long time observation while making an experiment in many protein chip image. So the feature extraction is needed by a simulator. In the case of feature analysis for macro array scan image the efficiency is maximized. In the fluorescence scan image, the response for each cell have been depend on R, G, B distribution of color image. But it is difficult to be classified as one color feature in the case of mixed color image. In this paper, the response color of a protein chip is classified according to the fuzzy integral value with respect to fuzzy measure as the user desired color. The result of the experiment for the macro array fluorescence image with the Scan Array 5000 shows that the proposed method using the fuzzy integral is important fact to be make decision for the ambiguous color.

• Korean Journal of Microbiology
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• v.48 no.2
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• pp.57-65
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• 2012

Phosphorous is an essential element for the synthesis of various biomolecules including phospholipids, carbohydrates and nucleic acids. Bacterial cells can uptake it as forms of phosphate and phosphate-containing nutrients from extracellular environments, and reserve extra phosphate to polyphosphate inside the cell. Among five phosphate transport systems, Pst plays central roles in phosphate transport, and its expression is coordinated by the regulation of PhoB-PhoR two component signal transduction system in response to extracellular levels of phosphate. Genomic studies on the response regulator PhoB reveal many genes independent of phosphate metabolism. Based on recent findings on phenotypes of bacteria lacking proper function of each phosphate transport system, this review discusses roles of phosphate transporters in maintaining optimum intracellular phosphate levels, and presents diverse phenotypes of phosphate transporters related with other environmental signals as well as phosphate, then finally points out functional redundancy among phosphate transport systems or their regulators, which emphasize importance of phosphate homeostasis in governing metabolism, adaptation, and virulence of bacteria.

• Journal of Korean Society of Environmental Engineers
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• v.29 no.6
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• pp.678-683
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• 2007

We isolated a toluene-degrading bacterium, TDB-4, from a bioreactor which designed to remove volatile organic compounds (VOCs) from the contaminated air. Based on the results of 16S rRNA gene analysis, it was classified as Pseudomonas sp. The toluene degradability was estimated in the variable toluene and bacterial concentrations. The bacterial growth and degradation rate was higher in the samples supplied with 50 ${\mu}mole/vial$ of toluene than with 10 ${\mu}mole/vial$. It was decreased, however, in the samples with 100 ${\mu}mole/vial$, indicating that toluene inhibit the growth or degradation activity of TDB-4 at high concentration. When the degradation ability of other compounds was examined, TDB-4 can degrade other VOCs such as styrene, benzene, and xylene. These results will be helpful to optimize the operating conditions to improve the efficiency of a bioreactor in detoxification of VOCs.

• Journal of the Korean Chemical Society
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• v.41 no.2
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• pp.88-97
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• 1997

The silyltriflates$(Ph_{3-n}SiH(OTf)_n)$have been produced by the reaction of triphenylsilane and triflic acid$(CF_3SO_3H)$at low temperature. These highly reactive compounds are a valuable reagent for the synthesis of numerous new functional substituted silane derivatives. The reaction of silyltriflates with alkenyl- and alkynylmagnesium bromide as well as organolithium compounds gave new silanes$Ph_2SiHR(R=\;C(CPh,\;CH=CH_2,\;CH_2CH=CH_2,\; (CH_2)_2CH=CH_2,\;(CH_2)_3CH=CH_2)$in high yields. The hydrosilation of prepared alkenyl- and alkynylsilanesPh_2SiHR$in the presence of a platinum catalyst(Pt/C) at high temperature$(200{\circ}C)$gave carbosilane polymers$((Ph_2SiCH=CPh)_n$and$(Ph_2Si(CH_2)m)n;\;m=2∼4, n{\ge}10)$along with five- and six-membered silaalkane ring compounds derived from intramolecular hydrosilation reactions. All of the prepared compounds are confirmed by NMR, UV, IR and mass spectroscopy as well as elemental analysis.

• Korean Chemical Engineering Research
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• v.54 no.2
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• pp.255-261
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• 2016

Adsorption experiments of Acid Yellow 14 dye using activated carbon were carried out as function of adsorbent dose, pH, initial concentration, contact time and temperature. The equilibrium adsorption data were analyzed by Langmuir, Freundlich and Temkin isotherm model. The experimental data were best represented by Freundlich isotherm model. Base on the estimated Freundlich constant (1/n=0.129~0.212) and Langmuir separation factor ($R_L=0.202{\sim}0.243$), this process could be employed as effective treatment method. The heat of adsorption of Temkin isotherm model was 5.101~9.164 J/mol indicated that the adsorption process followed a physical adsorption. Adsorption kinetics experimental data were modeled using the pseudo-first-order and pseudo-second-order kinetic equation. It was shown that pseudo-second-order kinetic equation could best describe the adsorption kinetics. Base on the negative Gibbs free energy (-4.81~-10.33 kJ/mol) and positive enthalpy (+78.59 kJ/mol) indicate that the adsorption is spontaneous and endothermic process.

• Korean Journal of Materials Research
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• v.6 no.8
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• pp.768-778
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• 1996

Iron oxide (hematite, $\alpha$-${Fe}_{2}{O}_{3}$) particles were prepared directly from aqueous solution using a crystal growth controller. Paticles properties and reaction mechanisum of products as a function of basicity, formation process and mechanism of needle-lkie hematite were investigated. hexagonal hermatite particles were formed in teh range below pH 9.0, ellipsoidal or rectangular hematite particles in the range of pH 10.75-11.75 respectively. In the range above pH 12.50, acicular $\alpha$-FeOOH was formed. Basicity of product solution produced in the range of pH 10.7511.75 was increased slightly as compared with basicity of reastants due to hydroxly ion(OH-) formed by dissociation crystal growth controller. Citric acid which is acted as a crystal growth controller was adsorbed in the form of itrate anion(R-COO-) on the ferric hydroxide and exerted important role on the formation to the needle-like $\alpha$-${Fe}_{2}{O}_{3}$ particles in this reaction system.

• Journal of the Korean Society of Propulsion Engineers
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• v.24 no.6
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• pp.69-77
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• 2020

A quasi one-dimensional multi-phase reaction flow analysis code is developed for the performance analysis of liquid pintle thrusters. Unsteady flow field, droplet evaporation, finite reaction and film cooling models are composed as the major models of the performance analysis. The droplet vaporization takes account of Abramzon's vaporization model, and the combustion employs a flamelet model based on detail chemical reactions. Shine's model is applied for the film cooling calculation. To verify each model, the Sod shock tube, single droplet vaporization, kerosene droplets combustion, and film length are evaluated.

• Parasites, Hosts and Diseases
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• v.21 no.1
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• pp.27-31
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• 1983

21명의 간흡충 감염환자 및 15명의 건강인으로부터 혈청을 수집하여 혈청내 IgE와 간흡충에 대한 특이 IgE 항체를 RIST와 RAST법을 이용하여 측정하였다. 간흡충 감염환자 및 건강인의 혈청 IgE와 특이 IgE는 각각 2,372 lU/ml과 364IU/ml 그리고 52.0%와 4.4%로 간흡충 감염자에서 모두 유의하게 상승되어 있었으며 (p<0.001 및 p<0.01), 혈청 IgE의 상승과 특이 IgE의 상승은 상호 밀접한 관계가 있었고(r=0.9451), 혈청 IgE와 EPG(r=0.6056), 특이 IgE와 EPG(r=0.5693) 역시 상관 관계가 있었다. 이상의 결과로 보아 간흡충 감염은 인체 혈청내 IgE 및 특이 IgE항체를 상승시키며, IgE 및 간흡충에 대한 특이 IgK는 숙주-기생충간의 면역반응에 관여할 것으로 생각된다.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.20 no.2
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• pp.127-132
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• 1998

$Medpor^{(R)}$(porous polyethylene) Surgical Implants are used for the augmentation or restoration of bony contour in craniofacial defects. The purpose of this study is to evaluate the ingrowth of soft tissue and bone after application in calvaria of rats. The experiment was carried out in 60 rats. The reflected periosteum was resutured after implantation of $Medpor^{(R)}$ as a experimental site, while in the calvarial bone the reflected periosteum resutured without implantation as a control site. The histologic examination was performed after 1-, 2-, 4-, 8-, 12-, 24-weeks implantation in calvaria of rats. I concluded that there was abundant ingrowth of soft tissue and bone without any adverse tissue response and that it shows good stability.