• Korean Journal of Microbiology
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• v.46 no.2
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• pp.213-218
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• 2010

Lactate dehydrogenases (LDHs) have been highly focused for long time, due to their important roles in biochemical and metabolic pathways of cells. On the basis of genome-wide searching results, three putative LDH genes from Bacillus cereus ATCC 14579 genome have been PCR-amplified, cloned, and well-expressed in E. coli. All three BcLDH isozymes are supposed to share highly conserved catalytic amino acid residues in common $NAD^+$-dependent LDHs. Meanwhile, BcLDH1 consisting of 314 amino acids shares 86 and 49% of identities with BcLDH2 and 3, respectively. Interestingly, only BcLDH1 showed the converting activities between L-lactate and pyruvate in the presence of $NAD^+$ coenzyme, while the other isozymes are likely to have almost no activity. As a result, it was revealed that BcLDH1 can be a typical $NAD^+$-dependent L-lactate-specific dehydrogenase.

• Korean Journal of Veterinary Research
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• v.49 no.1
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• pp.45-57
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• 2009

The molecular genetic characterization of Aujeszky's disease virus (ADV) Yangsan strain (ADVYS), a Korean isolate, was investigated by analyzing the electrophoresis patterns and the physical maps of the viral DNA digested with various endonucleases. To establish DNA library for ADV-YS, twelve major BamHI restricted segments were cloned. Each location of the segments in the ADV genome was determined by sequence comparison with the sequences reported in Genbank and those sequences of the both termini of the segments. Physical maps were constructed based on the electrophoresis patterns of the digested viral DNA by restriction endonuclease and the results of Southern blot analyses with various DIG labeled probes originated from those of enzyme restricted segments of virulent (Shope) and avirulent (Bartha) strain. Comparing ADV-YS with a standard strain of Kaplan in the maps of restriction enzymes, following major respects were identified: (i) disappearance of BamHI restriction site between the first and second BamHI segments, (ii) creation of the BamHI restriction site in the fifth segment, and (iii) generation of the BglII site in the unique short (US) region. The genome of ADV-YS also contains a type 2 herpesvirus DNA molecule (in which the US region only inverts itself relative to the unique longregion) like all other ADV strains except Norden strain(type3), analyzed up to date. The size of the ADV genome estimated from the sizes of the restriction enzyme fragments, was approximately 145.3 kb (BamHI) or 145.4 kb (BglII). BamHI enzyme cleavage patterns were compared among the five Korean ADV isolates: Yangsan, Yongin, Dangjin, Jincheon and Iksan strains. Difference either in the number or in the size of the DNA fragments, suspected regions of termini of IR and TR, could be detected among all five strains.

• Journal of Gifted/Talented Education
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• v.23 no.1
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• pp.49-65
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• 2013

The purpose of this study is to investigate the longitudinal impact of the engagement of private tutoring on student achievement and the influence of the engagement of gifted education on the effect of private tutoring by applying hierarchical linear growth modeling technique on the KELS (Korean Education Longitudinal Study) data. For the analysis, we used KELS data including the score of Mathematics achievement from 2005 to 2007 in order to get useful variables like whether or not to engage private tutoring, weekly time and monthly cost of private tutoring, the experience of gifted education, gender, prior achievement of 6th grade, parents' monthly income, internal motivation and self-efficacy. The sample consisted of 4,435 7th graders from Korean middle schools who were included in the KELS and have the data available to a significant dataset having no missing values include. The result suggest that the private tutoring experience predicted students' high Mathematics achievement and the interaction between grade and private tutoring time and cost has negative impact on the mathematics achievement. In contrast, it was found that the experience of gifted education has negative impact on the growth of mathematics achievement, but positive impact on the effect of private tutoring cost on the mathematics achievement. In other words, the experience of gifted education shows affirmative impact on the effect of private tutoring cost on the mathematics achievement indirectly. On the basis of this results, we discussed deeply the effect of private tutoring on the Mathematics achievement and the influence and its meaning of the experience of gifted education on the effect of private tutoring.

• Korean Journal of Medicinal Crop Science
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• v.6 no.3
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• pp.188-192
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• 1998

The correlations between growth charateristics and root yield were investigated in 74 peony lines collected from chief producing place in Korea. Among these peony lines, single flower type was 61% and the rest were double type. And pink flower was in majority. Stem length, stem diameter and number of stem per plant were $50{\sim}60cm$, $7{\sim}8mm$ and $5{\sim}6$, respectively. Generally, disease tolerance and root yield were better in single flower type peonies than double type. Among single flower type peonies, the correlation coefficients between each characteristics and root yield were as follows : number of stem $(0.676^{**})$, number of roots $(0.646^{**})$, stem length $(0.617^{**})$, root diameter $(0.590^{**})$ and root length $(0.443^{**})$. Correlation between root yield and paeoniflorin content was highly significant $(0.383^{**})$ among single flower type peonies and significant $(-0.382^*)$ among double flower type peonies. Correlation between stem diameter and root yield was not significant (0.066).

• Journal of Music and Human Behavior
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• v.12 no.2
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• pp.1-18
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• 2015

The purpose of this study was to compare the vocal characteristics of 40 adults with and without cerebral palsy (CP), upon presentation of three speech tasks (i.e., reading, chanting, and singing). The Praat program was utilized to generate data on fundamental frequency, voice intensity, jitter, and shimmer. The results of the analysis revealed no significant differences on fundamental frequency and intensity. However, both groups showed significant decreases in jitter and shimmer when engaged in singing tasks. The analysis of group differences indicated that adults with CP showed significantly higher variation scores on jitter and shimmer than the group without CP, and the difference on jitter and shimmer became greater during rhythmic chanting. In terms of jitter variation, the interaction effects according to the groups and types of speech tasks were greater, demonstrating the differences between the two groups. This study can be utilized as a basic research, regarding changes in vocal characteristics of adults with CP according to different musical speech tasks.

• Journal of Korean Society of Forest Science
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• v.85 no.4
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• pp.571-576
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• 1996

The geological and hydrological survey was carried out in Hyuseok-dong landslide area(10 ha), Youngchoon-myeon, Danyang-gun, Choongcheongbuk-do. The results showed that the bed rocks in this area consisted of shale, sandstone and limestone, and the piles of stones debris were also scattered around the hill above the study area. Soil texture was sandy loam, and bulk density of the soil in the lower part of the area was higher than that of the upper part, but void ratio of the soil in the lower part was lower than that of the upper part. Subsurface water was springing out to the surface in many places in this area. In this area, the storms over 70mm/day were frequent during the summer period from June through September. It was concluded that the occurrence of landslide had a close relationship with the geological characteristics, hydrological conditions and the rainfall distribution.

• Sleep Medicine and Psychophysiology
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• v.21 no.1
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• pp.29-32
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• 2014

Objectives: The aim of current study is to evaluate the relationship between sleep, suicide and serotonin using some scales and loudness dependence of auditory evoked potentials (LDAEP). Methods: Total 65 patients who met the criteria for major depressive disorder were enrolled in current study. The patients were divided into two subgroups according to their insomnia and a history of suicide attempts. The auditory event-related potentials were measured to evaluate LDAEP before beginning antidepressants. Results: The scores of total Beck Depression Inventory (BDI) and BDI item 9 (suicide) were higher in insomnia subgroup than non-insomnia subgroup (respectively, p=0.0033 and p=0.03). However, LDAEP did not differ each other. The subgroup with a history of suicide attempts had a higher score of BDI item 9 than the subgroup without a history of suicide attempts (p=0.00012). There was a tendency for the LDAEP to be higher in the subgroup with a history of suicide attempts ($1.39{\pm}0.94{\mu}V$) than the sub-group without a history of suicide attempts ($1.05{\pm}0.75{\mu}V$), although the difference was not statistically significant (p=0.078). Conclusion: Suicidality was related to insomnia. In addition, there was a tendency for serotonin activity to be lower in the subgroup with a history of suicide attempts. In future, more studies are needed.

• Journal of the Korean Geotechnical Society
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• v.32 no.11
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• pp.5-19
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• 2016

This paper presents the results from three-dimensional finite element (FE) analysis undertaken to provide insight into the lateral behaviors of piled gravity base foundation (GBF) for offshore wind turbine. The piled GBF was originally developed to support the gravity based foundation in very soft clay soil. A GBF is supported by five piles in a cross arrangement to achieve additional vertical bearing capacity. This study considered four different cases including a) single pile, b) three-by-three group pile (with nine piles), c) cross-arrangement group pile (with five piles), and d) piled GBF. All the cases were installed in homogenous soft clay soil with undrained shear strength of 20 kPa. From the numerical results, p-y curves and thus P-multiplier was back-calculated. For the group pile cases, the group effect decreased with increasing the number of piles. Interestingly, for the piled GBF, the P-multipliers showed a unique trend, compared to the group pile cases. This study concluded that the global lateral behaviour of the piled GBF was influenced strongly by the interaction between GBF and contacted soil surface.

• Korean Chemical Engineering Research
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• v.43 no.2
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• pp.187-201
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• 2005

Bioprocessing technologies utilizing 'biorecognition' between a solid matrix and a protein is being widely experimented as a means to replacing the conventional, solution-based technology. Frequently the matrices are chromatographic resins with specific functional groups exposed outside. Since the reactions of and interactions with the proteins occur as they are attached to the solid matrix, this 'solid-phase' processing has distinct advantages over the solution-phase technology. Solid-phase refolding of inclusion body proteins uses ion exchange resins to adsorb denaturant-dissolved inclusion body. As the denaturant is slowly removed from the micromoiety around the protein, it is refolded into a native, three-dimensional structure. Once the refolding is complete, the folded protein can be eluted by a conventional elution technique such as the salt-gradient. This concept was successfully extended to 'EBA (expanded bed adsorption)-mediated refolding,' in which the denaturant-dissolved inclusion body in whole cell homogenate is adsorbed to a Streamline resin while cell debris and other impurity proteins are removed by the EBA action. The adsorbed protein follows the same refolding steps. This solid-phase refolding process shows the potential to improve the refolding yield, reduce the number of processing steps and the processing volume and time, and thus improve the overall process economics significantly. In this paper, the experimental results of the solid-phase refolding technology applied to several biopharmaceutical proteins of various types are presented.

• Korean journal of applied entomology
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• v.54 no.4
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• pp.437-442
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• 2015

This study, examined Chilocorus kuwanae for biological control of Saissetia coffeae. We measured basic developmental characteristics of C. kuwanae and its capability to prey on the second-instar larvae of S. coffeae by indoor breeding under the following conditions: $25^{\circ}C{\pm}2^{\circ}C$, humidity $70%{\pm}5%$, and day length 16L : 8D. The average daily consumption of the second-instar larvae of S. coffeae by C. kuwanae adults was approximately 77.5 larvae/day, which was significantly 2.8 and 2.9 times higher than consumption by Chrysoperla carnea and Cryptolaemus montrouzieri, respectively. During the nymphal period, C. kuwanae consumed a total of 659.0 second-instar larvae of S. coffeae. The interaction between C. kuwanae and the second-instar larvae of S. coffeae varied according to their densities, with the increase in C. kuwanae consumption rate noted to decelerate and gradually level off at the maximum, rsembling Holling's Type II functional response. Duration of egg to adult development varied from 21.1 days to nearly 27.9 days at temperatures of $25^{\circ}C{\pm}2^{\circ}C$. Hatchability, pupation rate, and adult emergence were greater than 72.3%, 77.8% and 83.3%, respectively.