• Journal of Life Science
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• v.28 no.1
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• pp.78-82
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• 2018

Detection of pathogenic microorganisms takes several days by conventional methods. It is necessary to assess microorganisms in a timely manner to reduce the risk of spreading infection. For this purpose, bacteriophages are chosen for use as a biosensing tool due to their host specificity, wide abundance, and safety. However, their lytic cycle limits their efficacy as biosensors. Phage proteins involved in binding to bacteria could be a robust alternative in resolving this drawback. Here, a fragment of tail protein J (residues 784 to 1,132) of phage lambda fused with 6X His-tag (6HN-J) at its N-terminus was cloned, overexpressed, purified, and characterized for its binding with microorganisms. The purified protein demonstrated a size of about 38 kDa in sodium dodecyl sulfate - polyacrylamide gel electrophoresis (SDS-PAGE) and bound with anti-His monoclonal antibodies. It bound specifically to Escherichia coli K-12, and not Salmonella typhimurium, Bacillus subtilis, or Pseudomonas aeruginosa in dot blotting. Binding of the protein to E. coli K-12 inhibited about 50% of the in vivo adsorption of the phage lambda to host cells at a concentration of $1{\mu}g/ml$ 6HN-J protein and almost 100% at $25{\mu}g/ml$ 6HN-J. The results suggest that a fusion viral protein could be utilized as a biosensing element (e.g., protein chips) for detecting microorganisms in real time.

• Science of Emotion and Sensibility
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• v.26 no.3
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• pp.149-160
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• 2023

This study develops a time-varying system-based noncontact fabric sensor that can measure cerebral blood-flow signals to explore the possibility of brain blood-signal detection and emotional evaluation. The textile sensor was implemented as a coil-type sensor by combining 30 silver threads of 40 deniers and then embroidering it with the computer machine. For the cerebral blood-flow measurement experiment, subjects were asked to attach a coil-type sensor to the carotid artery area, wear an electrocardiogram (ECG) electrode and a respiration (RSP) measurement belt. In addition, Doppler ultrasonography was performed using an ultrasonic diagnostic device to measure the speed of blood flow. The subject was asked to wear Meta Quest 2, measure the blood-flow change signal when viewing the manipulated image visual stimulus, and fill out an emotional-evaluation questionnaire. The measurement results show that the textile-sensor-measured signal also changes with a change in the blood-flow rate signal measured using the Doppler ultrasonography. These findings verify that the cerebral blood-flow signal can be measured using a coil-type textile sensor. In addition, the HRV extracted from ECG and PLL signals (textile sensor signals) are calculated and compared for emotional evaluation. The comparison results show that for the change in the ratio because of the activation of the sympathetic and parasympathetic nervous systems due to visual stimulation, the values calculated using the textile sensor and ECG signals tend to be similar. In conclusion, a the proposed time-varying system-based coil-type textile sensor can be used to study changes in the cerebral blood flow and monitor emotions.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.15 no.4
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• pp.965-972
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• 2011

As an aged population has increased in Korea, the number of patients with chronic disease has soared up as well. The rapid increase of the chronic disease triggers a need of new paradigm of healthcare. In terms of data transmission of healthcare system, a use of data-transmission protocol based on bluetooth could be dismal in application of healthcare due to its postponement of connection. On the contrary, WPAN is evaluated to be proper to support the application of healthcare in restricted geographic areas. In addition, the bluetooth, a base of the current wireless network, doesn't support a special mechanism to cope up with emergent patients because of its delayed connection among devices. Against this backdrop, this study aims to design an integrated interface of multi bio-sensing and suggest a measuring and monitering system for the patients with chronic illnesses by using Zigbee of WPAN as a sufficient bandwidth is anticipated owing to frequent deliveries of complicated biological signals.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.11 no.3
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• pp.119-126
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• 2017

In the clinical setting, the pressure bio-feedback device is used for the spinal rehabilitation of patients with back pain, but it has several disadvantages. The purpose of this study was to develop a digitalized pressure biofeedback system that provides precise exercise method and posture in real time during the spinal rehabilitation exercise by sensing and monitoring body movements and balance of users and providing biofeedback to users. After that, the usability testing for a digitalized pressure biofeedback system will be conducted to identify problems such as safety, performance, operability, and satisfaction, and suggest improvement directions. A total of 33 subjects were participated in the usability testing. The experts group and the users group evaluated the developed digitalized pressure biofeedback system on a scale of 5 points after using the equipment. In the user group, safety was 3.59, operability was 4.38, satisfaction was 4.49. In the expert group, safety was 2.86, operability was 3.91, and performance was 4.28. Based on the usability evaluation, if the problems of stability of the cradle for tablet PC, air injection, screen display, etc. are solved, it becomes a exercise device capable of accurately exercising and evaluating the function of the spine by checking its own motion state while the spinal stabilization exercise.

• Journal of the Korean Magnetics Society
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• v.17 no.4
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• pp.172-177
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• 2007

In order to detect of the magnetic property in the cell unit, we studied the GMR-SV (giant magnetoresistance-spin valves) biosensor, which was depended on the micro patterned features according to two easy directions of longitudinal and transversal axes. Here, the multi layer structure was glass/NiO/NiFe/CoFe/Cu/CoFe/NiFe. The uniaxial anisotropy direction was applied to the patterned biosensor during the deposition and vacuum post-annealing at $200^{\circ}C$ under the magnitude of 300 Oe, respectively. Considering the magnetic shape anisotropy effect, the size of micro patterned biosensor was a $2{\times}5{\mu}m^2$ after the photo lithography process. By our experimental results, we confirmed that the best condition of GMR-SV biosensor should be the same direction of the axis sensing current and the easy axis of pinned NiO/NiFe/CoFe triple layer oriented to the width direction of device, and the direction of the easy axis of free CoFe/NiFe bilayer was according to the longitudinal direction of device.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.45 no.2
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• pp.1-7
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• 2008

In this paper, we have designed a millimeter-wave passive imaging sensor that is able to use remote sensing and security applications. The brightness temperature distribution of a scene is measured with a antenna at an angular resolution of $3^{\circ}$. The sensor is controlled by a PC, achieving a fast performance by using a pan/tilter. The pan/tilter should be able to scan a 2-D image of the scene, with a linear raster scan pattern. The mechanical scans in azimuth and elevation whereby an image of $20{\times}20$ pixels is acquired within less than 400s. Raw images are immediately displayed and stored for postprocessing.

• Proceedings of the KIEE Conference
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• 2008.07a
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• pp.2257-2258
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• 2008

본 논문에서는 최근 자신의 몸과 건강에 대한 관심이 증가함에 따라 WSN과 ZIgbee를 이용하여 언제 어디서나 운동하는 사람(이하 사용자)의 건강상태를 체크할 수 있는 헬스케어시스템을 구현하였다. 구현된 시스템은 사용자를 인식하는 RFID리더기와 운동량을 측정하기 위한 센서그룹과 사용자의 혈압, 심전도, 체온, 맥박 등을 수집하는 바이오센서, 센싱 된 정보를 수집하여 전달하는 게이트웨이, 사용자정보, 수집정보와, 측정정보를 저장하는 헬스케어관리서버 그리고 측정결과를 사용자가 모니터할 수 있는 모니터프로그램으로 구성된다. 구현된 센서노드는 Zigbee 프로토콜을 이용하여 센서네트워크를 구성한다. 그리고 리눅스 기반의 임베디드 시스템을 윈도우 기반에서 사용하기 위해 Cygwin을 이용한다. 수집된 정보는 USN과 WSN 로 실시간으로 전송된다. 이 전송된 결과 값을 헬스케어관리서버에서 저장, 관리하며 긴급 상황 발생 시 모니터를 통해 경보음을 내보내는 동시에, 사용자의 상태를 병원이나 소방서와 같은 응급처리 센터에 전송하도록 설계되었다. 구현결과 Zigbee 프로토콜과 WSN을 이용하여 사용자인식기반의 헬스케어시스템의 구현됨을 확인할 수 있었다.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.16 no.7
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• pp.1533-1540
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• 2012

u-Life care device collect body bio formation, and classify and store them in exercise patterns. Afterwards, the devices send the data through bluetooth wireless communication to the smart phones which set Google Android operation system at regular intervals. The information is checked out through application. u-Life care device calculates calories spent a day after monitoring activity quantity with 3-axis acceleration sensor. The device judges the status of health through body data mining and consults tailored exercise treatment. When sending body data, the device sends them in smart phone through Blue Tooth wireless communication at once. So, as a strong point, the device doesn't need mobile gateway or home gateway used for sending to web server information sensed from exercise life care products.

• Applied Chemistry for Engineering
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• v.27 no.6
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• pp.565-572
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• 2016

While there have been great demands on improving domestic water pollution issues, the necessity for real time monitoring of particular drug residues in water resources has been raised since drug residues including antibiotics could provoke new trains of drug-resistant bacteria in water environments. Among many different types of drugs used for pharmaceutical treatment, antibiotics are considered to be one of the most hazardous to our ecosystem since they can rapidly promote the spreading of drug-resistant bacteria in water environments. In this mini-review, we will highlight recent developments made on creating in-situ sensing platforms for the fast monitoring of antibiotic residues in aquatic environmental samples focusing on optical and electrochemical techniques. Related recent technology developments and the resulting economy effects will also be discussed.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.12 no.11
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• pp.1939-1946
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• 2008

The Oceanic ecosystem destruction caused by Water-Bloom, Jellyfish development, and Oil contamination in nation's waters becomes serious problem. For these reasons, the oceanic conditions are observed in air and on the water using helicopters and vessels. But, employing helicopters and vessels are limitted on a budget, and weather conditions. To overcome these problems, an unattended automated monitoring system not only gather all sorts of the oceanic condition in spite of bad weather, but save labor and oil costs. For each sensor connected in network and radiodetermination, location of sensors are verified in the tidal water to improve gathering informations.