• Journal of Life Science
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• v.32 no.2
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• pp.135-141
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• 2022

Thymosin β-4 (TB4) is a small peptide composed of 43 amino acids. To obtain sufficient biologically active mouse TB4 economically, we cloned and overexpressed this gene in an Escherichia coli system. With the isopropyl β-D-1-thiogalactopyranoside induction of the E. coli transformant, TB4 fusion protein with intein- and chitin-binding domain was successfully expressed in the soluble fraction within the E. coli cell. The TB4-intein - chitin-binding domain fusion protein was purified from the soluble fraction of E. coli cell lysate. The affinity chromatography with chitin beads and dithiothreitol-mediated intein self-cleavage reaction releases the TB4 peptide into the stripping solution. Sodium dodecyl sulphate - polyacrylamide gel electrophoresis and Western blot analyses were used to confirm that the recombinant TB4 peptide was produced with the expected size of 5 kDa. We found that the recombinant TB4 stimulated cell migration in the transwell plate chamber assay. After 18 hr of the treatment of the recombinant TB4 with 1 ng/ml concentration, the migration of the HT1080 cell was increased by 20% compared with that of the chemically synthesized TB4. The recombinant TB4 was also observed to promote the healing of a wound area in C57BL/6 mice by as high as 35% compared with that of the chemically synthesized TB4. These results suggest that the recombinant TB4 has better biological activity for cell migration and wound healing than that of the chemically synthesized TB4 peptide.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.19 no.2
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• pp.323-335
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• 2015

Mobile healthcare is a fusion of information technology and biotechnology and is a new type of health management service to keep people's health at anytime and anywhere without regard to time and space. The WBAN(Wireless Body Area Network) technology that collects bio signals and the data analysis and monitoring technology using mobile devices are essential for serving mobile healthcare. WBAN consisting of users with mobile devices meet another WBAN during movement, WBANs transmit data to the other media. Because of WBAN conflict, several nodes transmit data in same time slot so a collision will occur, resulting in the data transmission being failed and need more energy for re-transmission. In this thesis, we proposed a MAC protocol for WBAN with mobility to solve these problems. First, we proposed a superframe structure for WBAN. The proposed superframe consists of a TDMA(Time Division Muliple Access) based contention access phase with which a node can transmit data in its own time slot and a contention phase using CSMA/CA algorithm. Second, we proposed a network merging algorithm for conflicting WBAN based on the proposed MAC protocol. When a WBAN with mobility conflicts with other WBAN, data frame collision is reduced through network reestablishment. Simulations are performed using a Castalia based on the OMNeT++ network simulation framework to estimate the performance of the proposed superframe and algorithms. We estimated the performance of WBAN based on the proposed MAC protocol by comparing the performance of the WBAN based on IEEE 802.15.6. Performance evaluation results show that the packet transmission success rate and energy efficiency are improved by reducing the probability of collision using the proposed MAC protocol.

• Journal of Convergence for Information Technology
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• v.10 no.2
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• pp.89-95
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• 2020

Total polyphenol content and biological activities of water and methanol extract from Aronia melanocarpa berry, leaf, stem and twig were analyzed to investigate the potential for a bio-functional material. The polyphenol content of leaf and twig extracts were higher than those of berry and stem extracts. DPPH radical and ABTS radical scavenging activities of both extracts, were measured in order of twig, leaf, berry and stem. Tyrosinase inhibitory activities of water extract were measured in order of berry, leaf, twig and stem. Those of methanol extract were measured in order of twig, leaf, berry and stem. The nitrite-scavenging ability of water and methanol extract were measured in order of berry, leaf, twig and stem. Consequently it was shown that the non-edible parts of aronia, leaf and twig, had antioxidant activities and nitric oxide scavenging activities. It is expected that these materials could be used as functional bio-materials in bio-health care products including cosmetic products.

• The Journal of the Convergence on Culture Technology
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• v.8 no.6
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• pp.605-614
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• 2022

In this study, oregano was extracted by supercritical extraction and hydrothermal extraction method. In vitro experiments such as antimicrobial and antioxidant activity test were performed. As a result of the disc diffusion method, only the supercritical extracts formed a clear zone. The MIC for S. aureus was found only in the supercritical fluid extracts and it was 1000 ㎍/mL. The hydrothermal extract's MIC is 125 ㎍/mL for C. acnes. Through biofilm inhibition assay, we found that the supercritical fluid oregano extracts inhibit the biofilm of S. aureus by more than 70% even at low concentrations of 125 ㎍/mL. On the other hand, the antioxidant ability of the hydrothermal extract was better than that of the supercritical fluid extracts. Furthermore, we tried to make a skincare ingredient for atopic dermatitis by utilizing the S. aureus biofilm inhibitory ability of oregano supercritical fluid extracts. Liposome was used to overcome the low solubility of the oregano supercritical fluid extracts and increase stability.

• Journal of IKEEE
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• v.22 no.1
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• pp.21-28
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• 2018

In this paper, we have developed the intellectual kegel trainer with the bio-feedback. The one is smart health care system that can treat an evacuation impediment and urination polyuria with the low frequency stimulation module. Then this detects the shrinkage power of the pelvis muscles and correlates an smart phone entertainment application program for the users. In order to recover the function of pelvic muscles, we use the intellectual smart health care trainer with several biofeedback function. Such a trainer makes to strength the weakness pelvic muscles by biofeedback training and get exercise the pelvic muscles in physical suppress. The biofeedback training against the physical suppress can have strengthen the pelvic muscles and can display the operation graph of strengthen movement in monitor. Finally, It can be ensured the safety through EMI and performance test.

• Journal of Dairy Science and Biotechnology
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• v.41 no.1
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• pp.9-25
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• 2023

Yogurt fermentation is known to be beneficial because it provides a low pH and harsh environment for foodborne pathogens and improves organoleptic properties. Additionally, organic acids produced through fermentation have a good effect on the viscosity and gelling properties of yogurt. Several potential health benefits of probiotic and generally recognized as safe strains have been suggested. Yogurt is the preferred vehicle for delivering probiotics to health-conscious consumers. Therefore, manufacturers of probiotic beverages must comply with the relevant regulations. The development of probiotic yogurt begins with the selection of strains with safety and functional properties of probiotics. The selected probiotic strain should be technically suitable for viability and improve organoleptic quality while maintaining the number of bacteria above the standard value during processing and storage conditions. In addition, the efficacy of probiotic strains contained in yogurt should be investigated, confirmed, and approved according to well-designed clinical trials. Although various methods are used to detect probiotic strains, the recently widely used next generation sequencing method can be actively utilized. In the future, more research should be conducted with the latest methods to identify probiotic functions and accurately detect probiotic strains.

• Journal of the Korea Convergence Society
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• v.12 no.10
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• pp.343-352
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• 2021

A convergence study was conducted to develop a smartphone application for self-practice of core nursing skills and evaluate its usefulness for undergraduate nursing students. Mobile Application Rating Scale and seven essay questionnaire were used to for usability evaluation among 22 undergraduate nursing students. The score of the information domain was the highest with 4.19(SD 0.79). The subjective quality domain showed the lowest score of 3.08(SD 0.87). Participants' performance confidence score was 8.23(SD 1.60), and learning satisfaction score was 7.89(SD 0.87). Participants reported that the convenience and repetitive self-learning were the strengths of the app. In addition, design and technical supplementation, and lecturer-feedback would improve effectiveness of the current educational app. Findings of this convergent study would be helpful to promote the application of mobile apps for effective self-learning of core nursing skills in undergraduate nursing education. Future resesarch is needed to examine effectiveness study of mobile app on the performance of core nursing skills.

• Proceedings of the Korea Contents Association Conference
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• 2015.05a
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• pp.231-232
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• 2015

최근 유전체학, NGS(Next Generation Sequencing) 기술, IT/NT 장비의 발전 등에 따라 방대한 양의 바이오-메디컬 데이터가 생산되고, 이에 따라 빅데이터를 활용한 헬스케어 산업이 급속히 발달하고 있으며, 이와 관련된 빅데이터 기술은 국민의 건강 증대와 건강한 고령 삶을 제공하는 핵심 기술로 급부상하고 있다. 패스웨이는 단백질, 유전자, 세포 등의 생체적 요소 간의 역학관계 혹은 상호작용 등을 네트워크 형식으로 표현한 생물학적 심층지식으로, 바이오-메디컬 빅데이터 분석에 있어서 널리 활용되고 있다. 하지만 패스웨이는 매우 다양한 형태를 갖고 용량이 매우 큰 빅데이터로 이를 분석하는데 많은 시간이 소요된다. 그래서 본 논문에서는 세계적으로 가장 우수하고 방대한 양의 패스웨이를 제공하는 KEGG 패스웨이 데이터베이스로부터 사용자가 관심 갖는 패스웨이만을 자동 수집하고 패스웨이 간 계층구조를 기반으로 네트워크를 구성 후, 해당 패스웨이 네트워크에 대한 클러스터링과 핵심 패스웨이 선정을 통해 패스웨이 간의 역학관계 또는 상호작용을 직관적으로 분석할 수 시스템을 제안했다.

• Journal of Korea Society of Digital Industry and Information Management
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• v.18 no.1
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• pp.139-149
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• 2022

The study aims to understand the impact the consumption value changed by COVID-19 has on the purchasing behavior of industry foodservices dietitians and to provide baseline data in setting the marketing strategies for the food materials distribution companies in the future. The main survey was conducted for eight days from May 13th to 20th 2021 and 260 copies were collected and analyzed. Through the results of the study, the following conclusion elicited. First, consumption values that impact purchasing behaviors of industry foodservices dietitians are functional value and situational value. Second, for dietitians working at directly managed foodservice establishments, the higher the functional value of the food materials is, the higher the purchasing behavior. But such correlation was not found in the case of dietitians at consignment establishments. Third, compared to before and after COVID-19, the importance of functional value of food materials decreased, while the importance of situational value increased. Therefore, it is assumed that establishing marketing strategies differentiated by direct and consignment management will contribute to the energetic sales of the food material companies in the era of post COVID-19.

• Microbiology and Biotechnology Letters
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• v.52 no.1
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• pp.37-43
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• 2024

Recently, as the interest in makgeolli has increased and its products have become more diverse, the need to analyze the flavor of makgeolli has emerged. In this study, we analyzed the volatile flavor components in commercial makgeolli using an electronic nose. Discrimination function analysis suggested differences in flavor characteristics of commercial makgeolli depending on sterilization and type of fermentation starter. According to the variable importance point scores determined by partial least squares-discriminant analysis, it was revealed that sterilization, nuruk, and yeast profoundly influence the contents of ethyl acetate (fruity scent), acetaldehyde (strong odor) and isoamyl acetate (fruity scent), respectively. This result is expected to be a valuable stepstone to the characterization of makgeolli.