Cell Migration and Wound Healing Activities of Recombinant Thymosin β-4 Expressed in Escherichia coli |
Hong, Kyo-Chang
(Department of Smart Bio-Health, Dong-Eui University)
Choi, Yung Hyun (Department of Smart Bio-Health, Dong-Eui University) Kim, Gun-Do (Department of Microbiology, College of Natural Sciences, Pukyong National University) Cha, Hee-Jae (Department of Parasitology and Genetics, Kosin University College of Medicine) Jeon, Sung-Jong (Department of Smart Bio-Health, Dong-Eui University) Nam, Soo-Wan (Department of Smart Bio-Health, Dong-Eui University) |
1 | Kobayashi, T., Okada, F., Fujii, N., Tomita, N., Ito, S., Tazawa, H. and Hosokawa, M. 2002. Thymosin-β4 regulates motility and metastasis of malignant mouse fibrosarcoma cells. Am. J. Pathol. 160, 869-882. DOI |
2 | Kozaczuk, A., Selmi, A. and Bednarek, R. 2013. Bacterial expression, purification and angiogenesis-promoting activity of human thymosin β4. Protein Expr. Purif. 90, 142-152. DOI |
3 | Li, T., Ma, S. Y., Tang, X. C., Nie, L. Y. and Huang, H. 2013. Production and characterization of highly purified recombinant thymosin beta 4 in Escherichia coli. Protein Expr. Purif. 90, 90-95. DOI |
4 | Tsuji, Y., Kitahara-Tanabe, N., Noguchi, K., Gatanaga, T., Mizuno, D. and Soma, G. 1989. Production in Escherichia coli of human thymosin beta 4 as chimeric protein with human tumor necrosis factor. Biochem. Int. 18, 501-508. |
5 | Qiu, P., Kurpakus-Wheater, M. and Sosne, G. 2007. Matrix metalloproteinase activity is necessary for thymosin beta 4 promotion of epithelial cell migration. J. Cell. Physiol. 212, 165-173. DOI |
6 | Lv, S., Cai, H., Xu, Y., Dai, J., Rong, X. and Zheng, L. 2020. Thymosin-β 4 induces angiogenesis in critical limb ischemia mice via regulating Notch/NF-κB pathway. Int. J. Mol. Med. 46, 1347-1358. |
7 | Malinda, K. M., Goldstein, A. L. and Kleinman, H. K. 1997. Thymosin beta 4 stimulates directional migration of human umbilical vein endothelial cells. FASEB J. 11, 474-481. DOI |
8 | Malinda, K. M., Sidhu, G. S., Mani, H., Banaudha, K., Maheshwari, R. K., Goldstein, A. L. and Kleinman, H. K. 1999. Thymosin β4 accelerates wound healing. J. Invest. Dermatol. 113, 364-368. DOI |
9 | Martinez, A., Knappskog, P. M., Olafsdottir, S., Doskeland, A. P., Eiken, H. G., Svebak, R. M., Bozzini, M., Apold, J. and Flatmark, T. 1995. Expression of recombinant human phenylalanine hydroxylase as fusion protein in Escherichia coli circumvents proteolytic degradation by host cell proteases. Isolation and characterization of the wild-type enzyme. Biochem. J. 306, 589-597. DOI |
10 | Philp, D., Goldstein, A. L. and Kleinman, H. K. 2004. Thymosin β 4 promotes angiogenesis, wound healing, and hair follicle development. J. Coloproctol. 125, 113-115. |
11 | Renga, G., Oikonomou, V., Stincardini, C., Pariano, M., Borghi, M., Costantini, C., Bartoli, A., Garaci, E., Goldstein, A. L. and Romani, L. 2018. Thymosin β4 limits inflammation through autophagy. Expert Opin. Biol. Ther. 18, 171-175. DOI |
12 | Sosne, G. 2018. Thymosin beta 4 and the eye: the journey from bench to bedside. Expert Opin. Biol. Ther. 18, 99-104. DOI |
13 | Cha, H. J., Jeong, M. J. and Kleinman, H. K. 2003. Role of thymosin β4 in tumor metastasis and angiogenesis. J. Natl. Cancer Inst. 95, 1674-1680. DOI |
14 | Belsky, J. B., Rivers, E. P., Filbin, M. R., Lee, P. J. and Morris, D. C. 2018. Thymosin beta 4 regulation of actin in sepsis. Expert Opin. Biol. Ther. 18, 193-197. DOI |
15 | Bock-Marquette, I., Saxena, A., White, M. D., DiMaio, J. M. and Srivastava, D. 2004. Thymosin β4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair. Nature 432, 466-472. DOI |
16 | Carpintero, P., Anadon, R., Diaz-Regueira, S. and GomezMarquez, J. 1999. Expression of thymosin β 4 messenger RNA in normal and kainate-treated rat forebrain. Neuroscience 90, 1433-1444. DOI |
17 | Dai, B., Sha, R. N., Yuan, J. L. and Liu, D. J. 2021. Multiple potential roles of thymosin β4 in the growth and development of hair follicles. J. Cell Mol. Med. 25, 1350-1358. DOI |
18 | Esipov, R. S., Makarov, D. A., Stepanenko, V. N. and Miroshnikov, A. I. 2016. Development of the intein-mediated method for production of recombinant thymosin β4 from the acetylated in vivo fusion protein. J. Biotechnol. 228, 73-81. DOI |
19 | Xu, T. J., Wang, Q., Ma, X. W., Zhang, Z., Zhang, W., Xue, X. C., Zhang, C., Hao, Q., Li, W. N., Zhang, Y. Q. and Li, M. 2013. A novel dimeric thymosin beta 4 with enhanced activities accelerates the rate of wound healing. Drug Des. Devel. Ther. 7, 1075-1088. |
20 | Wang, X., Yang, G., Li, S., Gao, M., Zhao, P. and Zhao, L. 2013. The Escherichia coli-derived thymosin β4 concatemer promotes cell proliferation and healing wound in mice. Biomed. Res. Int. 2013, Article ID 241721. |
21 | Yu, R., Cao, S., Liu, Y., Si, X., Fang, T., Sun, X., Dai, H., Xu, J., Fang, H. and Chen, W. 2018. Highly effective biosynthesis of N-acetylated human thymosin β4 (Tβ4) in Escherichia coli. Artif. Cells Nanomed. Biotechnol. 46, S95-S104. DOI |
22 | Zhou, Q., Jiang, S., Ma, K. and Li, C. 2014. Expression of a novel recombinant fusion protein BVN-Tβ4 and its effects on diabetic wound healing. J. Biosci. Bioeng. 118, 341-343. DOI |
23 | Li, X., Zheng, L., Peng, F., Qi, C., Zhang, X., Zhou, A. and Wu, S. 2007. Recombinant thymosin beta 4 can promote full-thickness cutaneous wound healing. Protein Exp. Purif. 56, 229-236. DOI |
24 | Gomez-Marquez J., Dosil, M., Segade, F., Bustelo, X. R., Pichel, J. G., Dominguez, F. and Freire, M. 1989. Thymosinbeta 4 gene, preliminary characterization and expression in tissues, thymic cells, and lymphocytes. J. Immunol. 143, 2740-2744. DOI |
25 | Kaur, H. and Mutus, B. 2012. Platelet function and thymosin β4. Biol. Chem. 393, 595-598. DOI |
26 | Freeman, K. W., Bowman, B. R. and Zetter, B. R. 2011. Regenerative protein thymosin β-4 is a novel regulator of purinergic signaling. FASEB J. 25: 907-915. DOI |
27 | Kaveh-Baghbaderani, Y., Blank-Shim, S. A., Koch, T. and Berensmeier, S. 2018. Selective release of overexpressed recombinant proteins from E. coli cells facilitates one-step chromatographic purification of peptide-tagged green fluorescent protein variants. Protein Expr. Purif. 152, 155-160. DOI |
28 | Kim, S. H. and Kwon, J. K. 2014. Thymosin beta 4 improves dermal burn wound healing via downregulation of receptor of advanced glycation end products in db/db mice. Biochim. Biophys. Acta. 1840, 3452-3459. DOI |
29 | Otto, A., Muller, C., Huff, T. and Hannappel, E. 2002. Chemotherapeutic drugs change actin skeleton organization and the expression of β-thymosins in human breast cancer cells. J. Cancer Res. Clin. Oncol. 128, 247-256. DOI |
30 | Stoeckli, M., Chaurand, P., Hallahan, D. E. and Caprioli, R. M. 2001. Imaging mass spectrometry: a new technology for the analysis of protein expression in mammalian tissues. Nat. Med. 7, 493-496. DOI |
31 | Skosyrev, V. S., Kulesskiy, E. A., Yakhnin, A. V., Temirov, Y. V. and Vinokurov, L. M. 2003. Expression of the recombinant antibacterial peptide sarcotoxin IA in Escherichia coli cells. Protein Expr. Purif. 28, 350-356. DOI |