• Title/Summary/Keyword: 민들레속

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Analysis of Genetic Relationship Among Native Taraxacum and Naturalized Taraxacum species using RAPD (RAPD를 이용한 자생 민들레 종과 귀화 민들레 종간의 연관계 분석)

  • 안영희;박대식;정규환
    • Korean Journal of Environment and Ecology
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    • v.17 no.2
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    • pp.169-176
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    • 2003
  • The genetic relationships between 4 Korean native Taraxacum and 2 naturalized Taraxacum species were analyzed using the random amplified polymorphic DNA (RAPD) method. Because 141 polymorphic bands were generated from 30 random primers selected through the primer screening, it was possible to analyze the genetic relationship among 6 Taraxacum species. In RAED with the primer OPC12, OPD16, OPK16, OPK17, OPK20, OPS1 or OPS8, many specific polymorphic bands have been appeared in each species. Especially RAPD with the primer OPS8, a specific polymorphic band at 564bp was appeared only in the naturalized Taraxacum officinale. Based on RAPD analysis, Korean native Taraxacum and naturalized Taraxacum species are divided into two groups. T. officinale and T. laevigatum are classified into group I which is a naturalized Taraxacum species group, and T. mongolicum, T. hallasanensis, T. ohwianum and T. coreanum are classified into group II which is a Korean native Taraxacum species group. The result from the RAPD method was very similar to the result from the Bootstrap method. From the examination of the physical characteristics of 6 Taraxacum species populated in Korea, flowering period of Taraxacum species in group I are longer than Taraxacum species in group ll, and the direction of involucral bract of Taruxacum species in the group I was also different comparing to the group ll. Because the flowering color, leaf direction, and the specificity of seed germination of T. coreanum were different compared to the other species in the group II, T. coreanum would be genetically divergent and showed the highest dissimilarity index score.

Relationship of diploid East Aisan Taraxacum Wiggers using the capitulum morphological character (형태형질에 의한 동아시아산 민들레속 2배체 식물의 유연관계)

  • Lee, Kyung Hwa;Yang, Ji Young;Morita, Tatsuyoshi;Ito, Motomi;Pak, Jae-Hong
    • Korean Journal of Plant Taxonomy
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    • v.34 no.2
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    • pp.153-166
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    • 2004
  • Genus Taraxacum propagated through diploid sexual reproduction and polyploid agamospermy. The cluster analysis of Korean(2 species, 4 population), Japanese (2 species 4 taxa 6 population) and Taiwanese (1 species, 3 population) Taraxacum species using 15 measured capitulum morphological characters was conducted to study the speciation of diploid Taraxacum in East Asia. We measured 15 capitulum morphological characters including length of capitulum, length and shape of outer-involucre, corniculate appandage. Within one population, these characters were very various and were overlapped. The result of cluster analysis using morphological character showed that all species were clustered into four groups (Korean species & T. japonicum group, T. platycarpum subspecies group, T. platycarpum subsp. hodense group, T. formosanum group). Korean species, T. hallaisanense (diploid) and T. ohiwanum (diploid, triploid) were clustered into T. japonicum that was occurred in Kansai provinces of Japan. Therefore, we could infer that Korean species was closely related to Japanese T. japonicum of Japanese species.

Genetic Variation and Phylogenetic Relationship of Taraxacum Based on Chloroplast DNA (trnL-trnF and rps16-trnK) Sequences (엽록체 DNA (trnL-trnF, rps16-trnK) 염기서열에 의한 국내 민들레속 유전자원의 유전적 변이와 유연관계 분석)

  • Ryu, Jaihyunk;Lyu, Jae-il;Bae, Chang-Hyu
    • Korean Journal of Plant Resources
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    • v.30 no.5
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    • pp.522-534
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    • 2017
  • This study was investigated genetic variation in 24 Taraxacum accessions from various regions in South Korea based on the sequences of two chloroplast DNA (cpDNA) regions (trnL-trnF and rps16-trnK). T. mongolicum, T. officinale, and T. laevigatum were triploid, and T. coreanum and T. coreanum var. flavescens were tetraploid. The trnL-trnF region in native Korean dandelions (T. mongolicum, T. coreanum, and T. coreanum var. flavescens) were ranged from 931 to 935 bp in length, and that of naturalized dandelions were ranged from 910 bp (T. officinale) to 975 bp (T. laevigatum) in length. The rps16-trnK region in T. mongolicum, T. coreanum, T. coreanum var. flavescens, T. officinale, and T. laevigatum was 882-883 bp, 875-881 bp, 878-883 bp, 874-876 bp, and 847-876 bp, respectively, in length. The sequence similarity matrix of the trnL-trnF region ranged from 0.860 to 1.00 with an average of 0.949, and that of the rps16-trnK region ranged from 0.919 to 1.000 with an average of 0.967. According to the phylogenetic analysis, the Korean native taxa and naturalized taxa were divided independent clade in two cpDNA region. T. coreanum var. flavescens clustered only with T. coreanum, and there were no significant differences in their nucleotide sequences. The finding that two accessions (T. coreanum; Jogesan, T. mongolicum; Gangyang) had a high level of genetic variation suggests their utility for breeding materials.

First Report of Dandelion Leaf Gall Midge, Cystiphora sp. (Diptera: Cecidomyiidae), from Korea (한국에서 Cystiphora 속의 민들레잎혹파리(신칭) 발견 보고)

  • Lee, Heung Sik;Lee, Hee A;Lee, Young Su
    • Korean journal of applied entomology
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    • v.59 no.1
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    • pp.23-24
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    • 2020
  • ecently, dandelion leaf-gall midge of the genus Cystiphora, forming reddish-purple round bumps on dandelion (Taraxacum spp.) have been reported for the first time in Gyeonggi-do, Korea. Therefore, we report the observed area, damage pattern, and morphological characteristics of developmental stages.

A Study on the distribution and vegetation of Hypochoeris radicata community group in Jeju Island (제주도 개민들레군락군의 분포와 식생에 관한 연구)

  • 양영환;김문홍
    • Korean Journal of Plant Resources
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    • v.15 no.3
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    • pp.227-236
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    • 2002
  • The study was performed to understand the actual vegetation by using the phytosociological method on Hypochoeris radicata community group in Jeju-Island. 1. Tracheophyte belonged to the flora species is composed of 194 taxa which comprise 47 families,136 genera, 171 species, 22 varieties and 1 forma (Naturally plants 16 families, 47 genera, 67 taxa, including 64 species, 3 varieties). 2. According to the character species, the Hypochoeris radicata community group vegetation was classified into two communities; Erigeron annuus - Conyza sumatrensis, Setaria viridis var. pachystachs -Zoysia sinica community, distributed species. 3. This species is distributed from the shore to 1,700m the sea, on Jeju Island. 4. In terms of dominance and sociability of Hypochoeris radicata, the Seohong-dong, Seogwipo city site ranked highest site and the Seongsan-ri, Namjeju-gun site ranked lowest. 5. Catapodium rigidium which is belonged Gramineae was for the Hirst time found in JeJu.

Transformation of Taraxacum mongolicum Hand by Agrobacterium tumefaciens (Agrobacterium tumefaciens 에 의한 민들레의 형질전환)

  • 여상언;노광수
    • KSBB Journal
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    • v.16 no.5
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    • pp.480-485
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    • 2001
  • Genetic transformation in dandelion(Taraxacum mongolicum Hand). was studied. We used for transformation by Agrobacterium tumefaciens strian LBA4404 harboring a binary vector pBI121 carrying the CaMV 35S promoter-GUS gene fusion used as a reporter gene and NOS promoter-NPTII gene as a positive selection marker. To obtain transformed plants, leaf explants of dandelion were cocultured with Agrobacterium tumefaciens LBA4404 for 10 mins, then transferred to MS medium containing 1 $\mu$M IAA, 1$\mu$M BA, 100$\mu$g/ML carbenicillin and 50 $\mu$g/ML kanarmycin sulfate. After two weeks of subculture of the explants, Kanamycin-resistant shoots were formed on explants survived. When subjected to GUS histochemical assay, all of the regenerants showed the GUS-positive responses. Plantlets were be be transformed to soil for further growth.

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Two new naturalized species from Korea, Amaranthus hybridus and Crepis tectorum (한국 미기록 귀화식물: 긴털비름(Amaranthus hybridus)과 나도민들레 (Crepis tectorum))

  • Lee, You Mi;Park, Su Hyun;Jeong, Jae Min
    • Korean Journal of Plant Taxonomy
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    • v.35 no.3
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    • pp.201-209
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    • 2005
  • Two new naturalized species, Amaranthus hybridus and Crepis tectorum, are reported here. Descriptions and illustrations of the species and comparison of morphology with related species are provided. Amaranthus hybridus was first found at Nanjido, Seoul and is widely distributed in the central and southern part of Korean peninsula. New Korean name is given as "Ginteolbireum". It is well distinguished from the related species, A. retroflexus and A. patulus, by its late flowering time (September to October), sparse hair on the leaves, similar length between tepal and fruit, and long inflorescence without division. Crepis tectorum was first found at the entrance of Weoljeongsa Temple in Pyongchang-gun, Gangwon-do and spreads rapidly to other areas. New Korean name is given as "Nadomindulle".

A Study on Growth Inhibition of S. mutans by Lotus Leaf and Dandelion Extracts (연잎과 민들제 추출물에 의한 S. mutans 성장억제에 관한 연구)

  • Chi, Bo-Ram;Jo, Da-Young;Cha, So-Young;Chi, Min-Ji;Jeong, Hye-Won;Kang, Kyung-Hee
    • Proceedings of the KAIS Fall Conference
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    • 2011.12a
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    • pp.259-261
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    • 2011
  • 생약제제 중에서 연은 수련과의 식물로서 예로부터 위염, 출혈, 설사, 치질, 두통, 해독작용 등에 사용되어 왔으며, 민들레는 국화과에 속하는 식물로 천식, 해열, 강장, 부인병 등에 사용되어왔다. 최근에는 이들 생약제제의 약리작용에 대한 연구도 활발하게 이루어져 항산화작용, 항알레르기효과. 항균작용, 항암활성 등에 관한 연구가 보고되고 있다. 이에 본 연구에서는 연잎과, 민들레의 추출물이 치아우식 원인균인 S. mutans에 미치는 항균효과를 연구하고자 하였다. 추출물의 첨가에 따른 S. mutans의 성장억제율을 측정한 결과, 추출물의 농도가 높아질수록 S. mutans의 성장억제율도 높아지는 결과를 얻었다. 이로써 연잎과 민들레 추출물은 S. mutans의 성장을 억제하는 항균효과를 가지고 있음을 본 연구에서 확인 할 수 있었다.

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