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http://dx.doi.org/10.7732/kjpr.2017.30.5.522

Genetic Variation and Phylogenetic Relationship of Taraxacum Based on Chloroplast DNA (trnL-trnF and rps16-trnK) Sequences  

Ryu, Jaihyunk (Advanced Radiation Technology Institute, Korea Atomic Energy Research)
Lyu, Jae-il (Center for Plant Aging Research, Institute for Basic Science (IBS))
Bae, Chang-Hyu (Department of Plant Production Sciences, Graduate School of Sunchon National University)
Publication Information
Korean Journal of Plant Resources / v.30, no.5, 2017 , pp. 522-534 More about this Journal
Abstract
This study was investigated genetic variation in 24 Taraxacum accessions from various regions in South Korea based on the sequences of two chloroplast DNA (cpDNA) regions (trnL-trnF and rps16-trnK). T. mongolicum, T. officinale, and T. laevigatum were triploid, and T. coreanum and T. coreanum var. flavescens were tetraploid. The trnL-trnF region in native Korean dandelions (T. mongolicum, T. coreanum, and T. coreanum var. flavescens) were ranged from 931 to 935 bp in length, and that of naturalized dandelions were ranged from 910 bp (T. officinale) to 975 bp (T. laevigatum) in length. The rps16-trnK region in T. mongolicum, T. coreanum, T. coreanum var. flavescens, T. officinale, and T. laevigatum was 882-883 bp, 875-881 bp, 878-883 bp, 874-876 bp, and 847-876 bp, respectively, in length. The sequence similarity matrix of the trnL-trnF region ranged from 0.860 to 1.00 with an average of 0.949, and that of the rps16-trnK region ranged from 0.919 to 1.000 with an average of 0.967. According to the phylogenetic analysis, the Korean native taxa and naturalized taxa were divided independent clade in two cpDNA region. T. coreanum var. flavescens clustered only with T. coreanum, and there were no significant differences in their nucleotide sequences. The finding that two accessions (T. coreanum; Jogesan, T. mongolicum; Gangyang) had a high level of genetic variation suggests their utility for breeding materials.
Keywords
Chloroplast DNA sequences; Genetic variation; Phylogenetic relationship; Taraxacum;
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