Browse > Article

Analysis of Genetic Relationship Among Native Taraxacum and Naturalized Taraxacum species using RAPD  

안영희 (중앙대학교 산업과학대학 생물자원과학계열)
박대식 (중앙대학교 산업과학대학 생물자원과학계열)
정규환 (중앙대학교 산업과학대학 생물자원과학계열)
Publication Information
Korean Journal of Environment and Ecology / v.17, no.2, 2003 , pp. 169-176 More about this Journal
Abstract
The genetic relationships between 4 Korean native Taraxacum and 2 naturalized Taraxacum species were analyzed using the random amplified polymorphic DNA (RAPD) method. Because 141 polymorphic bands were generated from 30 random primers selected through the primer screening, it was possible to analyze the genetic relationship among 6 Taraxacum species. In RAED with the primer OPC12, OPD16, OPK16, OPK17, OPK20, OPS1 or OPS8, many specific polymorphic bands have been appeared in each species. Especially RAPD with the primer OPS8, a specific polymorphic band at 564bp was appeared only in the naturalized Taraxacum officinale. Based on RAPD analysis, Korean native Taraxacum and naturalized Taraxacum species are divided into two groups. T. officinale and T. laevigatum are classified into group I which is a naturalized Taraxacum species group, and T. mongolicum, T. hallasanensis, T. ohwianum and T. coreanum are classified into group II which is a Korean native Taraxacum species group. The result from the RAPD method was very similar to the result from the Bootstrap method. From the examination of the physical characteristics of 6 Taraxacum species populated in Korea, flowering period of Taraxacum species in group I are longer than Taraxacum species in group ll, and the direction of involucral bract of Taruxacum species in the group I was also different comparing to the group ll. Because the flowering color, leaf direction, and the specificity of seed germination of T. coreanum were different compared to the other species in the group II, T. coreanum would be genetically divergent and showed the highest dissimilarity index score.
Keywords
GENETIC RELATIONSHIP; TARAXACUM SPECIES; BOOTSTRAP;
Citations & Related Records
연도 인용수 순위
  • Reference
1 박수현(1995) 한국 귀화식물 원색도감. 일조각，346-349쪽
2 양효식 (1995) 서양민들레 생태형의 종간 경쟁에 있어 환경적인 변이 반응에 관한 연구. 한국환경생물학회지 13(2): 107-120
3 이영노, 오용자 (1969) 한국산 민들레속의 분류학적 연구. 한국생활과학연구원보고서 4: 63-68
4 내승준언(內勝俊彦)(1975) ンポポ속(屬)の 침입(侵入)と 정착(定着) について. 생물과학(生物科學) 27; 195-202
5 Nei, M. and Li, W. H.(1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. 76: 5269-5273   DOI   ScienceOn
6 Tinker, N. A., Fortin, M. G., and Mather, D. E.(1993) Random amplified polymorphic DNA pedigree relationships in spring barley Theor. Appl. Genet. 85: 976-984
7 Felsenstein, J.(1985) Confidence limits on phylogenies: an approach using the bootstrap Evolution, 39 : 738-791
8 Van De Peer, Y. and De Watcher, R.(1993) Treecon: A software package for the construction and drawing of evolutionary trees. Comput. Applic. Biosci. 9: 177-182
9 Williams, J. G. K., Hanafey, M. K., Rafalski, J. and Tingey, S.(1993) Genetic analysis using random amplified polymorphic DNA markers. Meth Enzymol. 218: 704-40   DOI
10 Suehiro, K., H. Ogawa and K. Hozumi(1986) Competition between two naturalized dandelions, Taraxacum officinale Weber and Taraxacum laevigatum Dc., in mixed cultures with different levels of soil moisture. Bot. Mag. Tokyo 99: 1-14   DOI
11 Benner, M. S., M. D. Braunstein and M. U. Weisberg(1995) Detection of DNA polymorphims within the genus Cattleya(Orchidaceae). Plant Mol. Biol. Rep. 13: 147-155   DOI   ScienceOn
12 안영희, 최광율 (2000) 자생 민들레류와 서양민들레 종자의 발아특성 차이 한국환경생태학회지 14(3): 199-204
13 이창복(1980) 대한식물도감. 향문사. 783-784쪽
14 Rani, V. A., Parida and S. N. Raina(1995) Randomly amplified polymorphic DNA(RAPD) markers for genentic analysis in micropropagated plants Populus deltoides Marsh. Plant Cell Reports 14: 459-462
15 강혜순, 최유미 (1998) 두 외래종 민들레 번식특성의 계절적 변이. 한국생태학회지 21: 475-486
16 박헌우, 박인근(1997) 경기도 서부일원의 민들레속 식물의 분포. 한국생태학회지 20: 1-8
17 이창복, 김윤식, 김장석, 이정석(1986) 신고 식물분류학. 향문사. 53-100쪽
18 Welsh, J., Honeycutt, R. J., McClelland, M., Sobral, B. W. S.(1991) Parentage determination in maize hybrids using the arbitrarily primed polymerase chain reaction(AP-PCR). Theor. Appl. Genet, 82: 473-476   DOI   ScienceOn
19 Chalmer, K. J., Wauugh, R., Sprent, J. K., Simons, A. J. and Powell, W.(1992) Detection of genetic variation between and within populations of Gliricidia sepium and G. maculata using RAPD markers. Heredity. 69: 465-72   DOI   ScienceOn
20 굴전만(堀田滿)(1989) 세계유용식물사전(世界有用植物事典) 평단사(平丹社)(東京). 1026-1027쪽
21 Dweikat, I., Mackenzie, S., Levy, M., and Ohm, H.(1993) Pedigree assessment using RAPD-DGGE in cereal crop species. Theor. Appl. Genet. 85: 496-505
22 삼전용의(森田龍義)(1976) 일본산(日本産), ンポポ屬の2倍體と倍數體の分布. 國立科學博物館報告2(1): 23-28
23 Halward, T., Stalker T., LaRue E., and Kochert G.(1992) Use of single-primer DNA amplification in genetic studies of peanut(Arachis hypogaea L.) Plant Mol. Biol. 18: 315-325   DOI   ScienceOn
24 Sneath, P. H. A. and Sokal, R. R. (1973) Numerical Taxonomy. W.H. Freeman(San Francisco). pp. 341-347