• Title/Summary/Keyword: 미생물 총 균수

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Microbial changes and physico-chemical properties of beef and pork loin with microwave treatment (마이크로파 처리한 소고기 및 돼지고기의 미생물 변화 및 이화학적 특성)

  • Lee, Kyong-Ae;Byoun, Kwang-Eui
    • Korean Journal of Human Ecology
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    • v.14 no.1
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    • pp.217-221
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    • 2005
  • In this study, the effect of microwave treatment on physico-chemical properties and microbial changes of beef and pork loin was investigated. Beef and pork were heated in microwave with an adjusted electric output at 500 W and operating frequency 2,450 MHz. The beef and pork samples were treated with microwave for 10 s or 20 s at 50% duty cycle. Drip losses and TBA values of beef and pork samples increased due to the microwave treatment. The samples became darker and more yellowish through microwaves, whereas their redness was unchanged. Microbial content of beef and pork samples declined as the treatment time increased. When pork samples were heated in a microwave oven, organisms were reduced by 2 log cycles in 20 s. It was concluded that a 20 second-long microwave treatment can great1y enhance the microbial safety of the pork sample.

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Effect of Electrolyzed Acid-Water on Initial Control of Microorganisms in Kimchi (전해산화수를 이용한 김치의 초기 미생물 제어 효과)

  • 정승원;박기재;김영호;박병인;정진웅
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.5
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    • pp.761-767
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    • 1996
  • To lessen the initial level of microorganisms, electrolyzed acid-water was used as washing and brine water in the manufacturing process. On the washing and salting processes, application of electrolyzed acid-water showed a possibility to lessen the microorganism level of Chinese cabbage effectively. Microbial level of Chinese cabbage was reduced to about 1/4 level by salting and washing process with electrolyzed acid-water while Chinese cabbage salted with tap water increased to about 1.7 times. And no coliform and E. coli were detected. However significant differences between seasoning mixtures prepared with electrolyzed acid-water and with tap water were not observed in microbial levels. Relatively low level of total count in kimchi prepared with electroyzed acid-water was kept until 15 days of fermentation at $10^{\circ}C.$ Any significant difference between them was not observed after 20 days of fermentation. pH and acidity were showed the same tendencies as microbial count.

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Quantitative Analysis of Microbiological Profiles of Retailed White Rice (시판 백미의 미생물학적 프로파일 정량분석)

  • Kim, Min-Ju;Kim, Byung-Hoon;Park, Sung-Soo;Park, Sung-Hee;Kim, Dong-Ho;Kim, Keun-Sung
    • Journal of Food Hygiene and Safety
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    • v.26 no.3
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    • pp.198-202
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    • 2011
  • Rice has been the most important staple food in everyday meals of Korean people for thousands of years. Nowadays, it is getting increasingly used as flour ingredients in a variety of processed foods, so that it is consumed in more diversified ways. As a consequence, production volume of rice flour to manufacture rice cakes, noodles, breads, or confectioneries is recently getting increased in Korea. But there are not sufficient research outcomes to guarantee Korean consumers microbiological qualities of rice flour as well as rice. As a preliminary experiment, therefore, the microbiological profiles (aerobic mesophilic bacteria (AMB), spore-forming aerobic bacteria (SAB), lactic acid bacteria (LAB), yeasts and molds (YM), and Escherichia coli and coliforms) have been monitored for nine retailed white rice samples in this study. AMB counts ranged $10^2-10^6$ CFU/g for all the nine white rice samples. All the nine rice samples have SAB counts within a narrow range $(1.0{\times}10^2-2.5{\times}10^3$ CFU/g). LAB was detected in two white rice samples ($4.0{\times}10^2$ and $3.7{\times}10^3$ CFU/g), YM was detected in one white rice sample ($2.0{\times}10^2$ CFU/g) only. E. coli was not detected from all the nine samples. Coliforms were detected in one white rice sample ($4.1{\times}10$ CFU/g) only. All the rice samples were conclusively considered to have various microorganisms, though most of them are harmless and some, such as coliforms, may be harmful.

Effect of the Herbicide Bentazon on Nitrification, and on Numbers of Bacteria and Fungi in the Soil (제초제(除草劑) Bentazon이 질산화작용(窒酸化作用) 및 토양미생물(土壤微生物)의 균수(菌數)에 미치는 영향(影響))

  • Kang, Kyu-Young
    • Applied Biological Chemistry
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    • v.21 no.2
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    • pp.81-83
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    • 1978
  • The effect of bentazon(3-isopropyl-1H-2,1,3-benzothiadiazine-(4)-3H-one-2,2-dioxide) at 50, 200ppm on nitrification of 100ppm applied $NH_4-N$, and on the numbers of nitrite-oxidizing bacteria, and on total bacterial and fungal populations were studied in a soil for six weeks. The herbicide retarded nitrification with increasing its treatment, which was coincident with the decrease of nitrite-oxidinzing bacterial populations. Bentazon treatment in a soil caused the decrease of total bacterial populations and inversely the increase of fungal populations for 2 weeks.

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Effect of Pine Needle(Pinus densiflora Seib. et Zucc) Sap on Kimchi Fermentation (솔잎즙의 첨가가 김치의 발효숙성에 미치는 영향)

  • Choi, Moo-Young;Choi, Eun-Jung;Lee, Eun;Cha, Bae-Cheon;Park, Hee-Juhn;Rhim, Tae-jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.25 no.6
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    • pp.899-906
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    • 1996
  • The physicochemical and microbiological studies were conducted to examine the effect of pineneedle(Pinu densinora Seib. et Zucc) sap on the Kimchi fermentation. Kimchi with the addition of various levels(0, 0.5, 1.0, or 1.5%) of pine needle sap was fermented either at $4^{\circ}C$ for 15 days after placing at room temperature for 24 hours(Group A) or at $15^{\circ}C$ for 15 days(Group B). pH reached the optimal value of Kimchi fermentation(pH 4.2) on day 3 and day 4~7 in 0% treatment and pine needle sap treatments, respectively, which indicated that shelf-life of Kimchi was extended by 1~4 days by the addition of pine needle sap. Total acidity was decreased by the addition of pine needle sap. More rapid decrease in pH and increase in total acidity were observed in Group B than in Group A. Reducing sugar content was reduced to approximately 80% by day 4~5 in all treatments. Total vitamin C content was reached peak on day 1 of fermentation and then decreased in all treatments. Reducing sugar and total vitamin C contents were slightly increased by the addition of pine needle sap due to the components present in pine needle sap. Total viable cell number rapidly increased to reach Peak on day 3 and then slowly decreased during the fermentation. However, total viable cell number as well as reducing sugar and total vitamin C contents did not differ between Group A and Group B. In Group A, Lactobacillus cell number in 0% treatment continued to increase to reach peak on day 9, while the numbers in pine needle sap treatments reached Peak on day 5~9 and then gradually decreased throughout the fermentation. Unlike in Group A, Lactobaillus cell numbers in pine needle sap treatments in Group B continued to increase to reach Peak on day 7. As pine needle sap levels increased, total viable cell number and Lactobacillus cell number decreased regardless of fermentation temperatures. The results of this study indicate that pine needle sap causes to delay the Kimchi fermentation by slowing down pH drop and inhibiting the Lactobacillus cell growth.

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라드를 이용한 열처리 온도 변화에 따른 ${\beta}-carotene$의 산화 안정성에 대한 연구

  • Won, Hye-Gyeong;Jeong, Sang-Hui;Hwang, Mi-Ja;Kim, Jin-Hui;Lee, Ji-Yeong;Park, Nam-Yeong;Lee, Chi-Ho
    • Proceedings of the Korean Society for Food Science of Animal Resources Conference
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    • 2005.05a
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    • pp.358-362
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    • 2005
  • 0%, 0.0012%, 0.004%의 ${\beta}-carotene$을 lard를 첨가한후 $80^{\circ}C$, $130^{\circ}C$, $180^{\circ}C$의 온도처리로 2분간 열처리를 하고 $50^{\circ}C$에서 보관시키면서 각 기간별로 총 균수검사, 과산화물가(Peroxide Value), TBArs를 측정하여 비교하였다. 1) 미생물 총 균수 실험에서 ${\beta}-carotene$의 첨가량이 0%, 0.0012%, 0.004%로 증가함에 따라 균의 증식이 둔화되었다. 2) Peroxide Value의 측정 실험에 ${\beta}-carotene$의 첨가량이 0.004%일 때가 0%, 0.0012%에서 보다 항산화 능력이 더 높은 것으로 나타났다. 3) TBA가 측정실험에서 control과 Sample과의 비교에서 온도가 증가함에 따라 그 증가폭 커지므로 ${\beta}-carotene$의 항산화 능력이 있다는 것을 알 수 있다.

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The Effects of Added Ripened Tomato on the Quality of Baechukimchi (완숙 토마토의 첨가가 배추김치의 품질에 미치는 영향)

  • Moon, Sung-Won;Park, Jung-Eun;Jang, Myung-Sook
    • Journal of the East Asian Society of Dietary Life
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    • v.17 no.5
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    • pp.678-688
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    • 2007
  • In this study, we investigated the effects of ripened tomato on the Baechukimchi fermentation, by measuring sensory, physicochemical, and microbiological properties during fermentation up to 28 days. The Baechukimchi, with various levels [0, 10, 20, 30%(w/v)] of ripened tomato, was fermented at $10^{\circ}C$. The product containing 20% ripened tomato showed the highest scores for overall acceptability, sour taste, texture, sour smell, and color. The intensity characteristic scores related to sour smell and sour taste were the highest in the control, and the texture score was highest in the 20% ripened tomato sample. During fermentation, total acidity increased while pH gradually decreased. The reducing sugar and total vitamin C contents of the experimental groups with added ripened tomato were higher than those of the control. Also, the time periods to reach maximum levels of lactic acid bacteria, by log numbers of cells and total viable cells, were more delayed in the experimental groups with added ripened tomato than in the control. Overall, the results indicate that a 20% addition of ripened tomato to Baechukimchi during fermentation can enhance sensory qualities and extended the palatable period of the product.

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Quality Changes of Salted Chinese Cabbages with Electrolyzed Water Washing and a Low Storage Temperature (전해수 세척 및 저장 온도에 따른 절임배추의 품질변화)

  • Park, Seong Soon;Sung, Jung Min;Jeong, Jin Woong;Park, Kee Jai;Lim, Jeong Ho
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.4
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    • pp.615-620
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    • 2013
  • To extend the shelf-life of salted Chinese cabbages, electrolyzed water (EW) was used to wash raw Chinese cabbages before the salting process (to control microbial growth), and different storage temperatures evaluated (0, 4, and $10^{\circ}C$). A tap water washing group (TW) was used for comparison. The initial total bacterial population was 5.36 log CFU/g in the TW treatment and 3.50 log CFU/g in the EW treatment. The EW treatment decreased bacterial numbers by approximately 2 log CFU/g compared to the TW treatment and kept this initial population number for 32 days at $0^{\circ}C$. The salinity had no difference during storage. In general, several factors (pH, reducing sugars, hardness, and transmittance) decreased over time, and decreased slowly with EW treatment and $0^{\circ}C$ storage. Overall, the salted Chinese cabbages with EW treatment showed lower bacterial populations compared to TW treatment, and when stored at $0^{\circ}C$, delayed decreases in quality.

Effect of microbial product made of Bacillus stearothermophilus DL-3 on microorganisms in soil and growth of lettuce and Chinese cabbage. (Bacillus stearothermophilus DL-3 미생물 제재의 처리가 토양 미생물상 및 상추와 배추의 생장에 미치는 영향)

  • 김순희;배계선;양재균;이유정;오주성;정순재;문병주;이진우
    • Journal of Life Science
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    • v.14 no.5
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    • pp.778-787
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    • 2004
  • Effect of the microbial product made of Bacillus stearothermophilus DL-3, which was isolated from the soil and identified in this study, and rice bran on microorganisms in soil and growth of lettuce (Red skirt lettuce) and Chinese cabbage (Ga rack new No.1 Chinese cabbage) was investigated. Total numbers of microorganisms in the pot with untreated soil, treated with standard amount of microbial product and treated with double amounts of microbial product for growth of lettuce after 6 weeks were 2.78${\times}$10$^{7}$ CFU/g, 2.72${\times}$10$^{8}$ CFU/g and 3.63${\times}$10$^{8}$ CFU/g. Total numbers of microorganisms in the soil without treatment of microbial product and treated with standard amount of microbial product were 2.06${\times}$10$^{8}$ CFU/g and 5.49${\times}$10$^{8}$ CFU/g. Total numbers of microorganisms in the pot with untreated soil, treated with standard amount of microbial product and treated with double amounts of microbial product for growth of Chinse cabbage after 6 weeks were 1.43${\times}$10$^{7}$ CFU/g, 3.42${\times}$10$^{8}$ CFU/g and 7.22${\times}$10$^{8}$ CFU/g. Total numbers of microorganisms in the soil without treatment of microbial product and treated with standard amount of microbial product were 5.75${\times}$10$^{8}$ CFU/g and 7.96${\times}$10$^{8}$ CFU/g. On basis of leaf length, leaf width, leaf number, wet weight and dry weight, the growth of lettuce and Chinese cabbage on the soil treated with microbial product was faster than that on the untreated soil. The treatment of microbial product in the soil resulted in the increase of useful microorganisms, which seemed to enhance the growth of lettuce and Chinese cabbage.

An Analysis Microbiological Contamination on the Facilities and Utilities of Japanese Restaurants (일식 레스토랑 설비와 조리기구의 미생물 오염 실태 분석)

  • Kim, Gi-Young;Park, Gye-Young;Chae, Myoung-Hee
    • Culinary science and hospitality research
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    • v.15 no.4
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    • pp.9-17
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    • 2009
  • The microbiological examinations was conducted for the hygienic evaluation on ten Japanese restaurants during summer season in Gwangju, Korea. Total two hundreds swabbed samples using sponge were collected from the surface of facilities and utensils at restaurants. The number of total microorganism, coliform, E. coli, Staphylococcus, Salmonella, and Listeria were measured. The results demonstrated that most swabbed samples were contaminated with microorganisms and coliforms. The degree of contamination depended on the sample sites. The severely contaminated sites were floor, trench, and working table for the fish and total counts of those samples were over $10^4$ CFU/100 $cm^2$. The coliforms were detected on the floor, trench, and wood cutting board over $10^3$ CFU/100 $cm^2$. Moreover, coliforms was detected on the towel, dish, and working place for fish and vegetables. The E. coli was detected on the floor and trench of several restaurants. The Staphylococcus spp. was detected on the cutting board, towel, floor and trench of several restaurants. The Salmonella spp. and Listeria spp. were not detected all over the samples. From those results showed that sanitation management and disinfection were required on the Japanese restaurants.

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