• Korean Journal of Microbiology
• /
• v.50 no.2
• /
• pp.137-151
• /
• 2014

Various studies have been conducted to investigate effects of dams on river ecosystems, but less information is available regarding damming impacts on downstream denitrification. We measured denitrification enzyme activity (potential denitrification rate) and denitrifier abundances (using nirS, nirK, and nosZ as markers) in dammed headstreams of the Nakdong River in South Korea. Sediments in Phragmites-dominated riparian areas and in-stream areas across streams (dammed vs. reference) with different streambed materials (gravel and sand) were sampled occasionally. We hypothesized that (i) the higher available N and C contents in sediments downstream of dams foster larger denitrifier communities than in the reference system and (ii) differences in potential denitrification rates across the systems correspond with denitrifier abundances. Despite 30 years of different hydrological management with dams and greater inorganic N and DOC contents in sediments downstream of dams, compared to the references, abundances of denitrifier communities and potential denitrification rates within the whole sediment were not significantly different across the systems. However, nirS and nosZ denitrifier abundances and potential denitrification rates were considerably increased in specific sediments downstream of dams (gravelly riparian and sandy in-stream) with regard to flooding events and seasonal temperature variation. nirK was not amplified in all sediments. Canonical correspondence analyses (CCA) revealed that the relationship between abundances of denitrifier communities and nutrient availabilities and potential denitrification rates was a weak one.

• Journal of Conservation Science
• /
• v.30 no.2
• /
• pp.169-179
• /
• 2014

After occurrence of Cyanobacteria in 1997, Songsan-ri tombs located in Gonju have been investigated to monitor for biological damage. The room temperature of Tomb No.6 was $18.6{\sim}19.8^{\circ}C$ and the relative humidity was 94.3~99.9%. The temperature of Royal Tomb of King Muryeong was $17.3{\sim}18.53^{\circ}C$ and the relative humidity was 73.2~96.45%. The variation of relative humidity increased after setting up air vents. If the outside temperature increases, dew condensation occurs on the floor and the north side. When conditioning equipment operates, the maximum temperature differences between walls is $2.8^{\circ}C$. Bacteria from the air of the tomb and on the surface of the walls outnumbered fungi. 20 species of fungi including Alternaria sp., Aspergillus sp., Penicillium sp., and 19 species of bacteria including Pseudomonas sp., Arthrobacter sp., are identified. Microbes in the tombs may damage cultural heritage. The growth possibility of microbes should be estimated because the microbes in the tombs may damage mural painting. The interrelation between microenvironmental condition and biological damage of mural painting should be researched to come up with an long-term conservation method.

• Journal of Conservation Science
• /
• v.33 no.4
• /
• pp.267-274
• /
• 2017

The Silleuksa temple in Yeoju city is adjacent to the South Han River and often has high humidity and fog days. In addition, its proximity to forests renders it prone to damage by insects and microorganisms. In order to obtain the basic data necessary for the establishment of a cultural property preservation management plan, the biological distribution of the wooden buildings and the termite distribution in the surrounding forests were monitored for three years. The wood-boring bee, an insect known to inflict damage to heritage structures, was confirmed, and the distribution of insects varied annually and seasonally, presumably due to the location characteristics of the site and the climate change during the year. In the forest behind, termite colonies were identified as a whole and their distribution pattern was different every year. As per the results of the microorganism survey, the species distribution and pollution degree were different at the entrance of the building. Therefore, it needs continuous biological distribution and conservation environment monitoring to mitigate the effects of wood-damaging organisms.were different according to the entrance of the building.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.25 no.4
• /
• pp.307-313
• /
• 1992

To study the seasonal variation of heterotrophic bacteria near the surrounding seawater of Porphyra forming area, samples were collected in the intertidal waters of Mokp'o of the Yellow Sea from February to December, 1990. Annual distribution of heterotrophic bacteria ranged from $7.5\times10^2\;to\;1.1\times10^5\;cfu/ml,$ annual distribution of physiological characteristic bacteria ranged from $5.0\times10\;to\;4.34\times10^4\;cfu/ml$ for proteolytic bacteria, from 0 to $1.35\times10^4\;cfu/ml$ for lipolytic bacteria and from 0 to $1.2\times10^4\;cfu/ml$ for amylolytic bacteria. Sixty-five percent of isolates from seawater were rods, and $76.7\%$ of isolates were Gram-negative. Most isolates were mesophiles and showed utilization of various carbon sources such as glucose, maltose, lactose, sucrose and arabinose. Most isolates also showed tolerance to a broad range of salt concentration. Dominant genus in seawater were Flavobacterium spp., in February, Moraxella spp., Acinetobacter spp. in March, Bacillus spp., Chromobacterium spp., Micrococcus spp., Vibrio spp. in July and Chromobacterium spp., Micrococcus spp., Bacillus spp. in November.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
• /
• 2003.10a
• /
• pp.148.2-149
• /
• 2003

고사리(Pteridium aquilinum)는 참고사리과에 속하는 다년생 양치식물로서 온대지방과 열대지방에 널리 분포되어 있으며 이른봄부터 늦가을까지 산야에 생육되며 북극 남아프리카 지역을 제외한 전세계에 광범위하게 자생하고 있다. 고사리는 봄철에 어린싹을 삶아 물에 담구었다가 먹거나 건조시켜 저장해서 이용하는데 현재 유통되고 있는 건고사리는 수확, 건조 및 저장 중에 비위생적인 관리 등에 의해 미생물의 증식 및 해충의 발생 등으로 품질이 크게 저하되어 저장상 많은 어려움을 안고 있다. 방사선에 의한 식품 저장은 이미 그 건전성과 경제적 타당성이 인정되어 세계 여러나라에서 실용화되고 있으며 특히 건조 채소류의 살균, 살충을 위한 방사선의 이용은 제품의 재수화성을 증가시킨다는 보고도 있다. 따라서, 본 실험에서는 국내에서 소비량이 비교적 많은 건고사리에 Co-60 감마선을 조사하여 건고사리 조리전 필수 조리조작인 불림조작 조건에 따른 복원력 및 물리적 특성을 분석하였다. 건고사리에 조사선량을 0, 3, 5, 7kGy로 달리 하여 조사하였고 침지온도, 침지 시간에 따른 수분함량, 부피, 수화 복원력, 색상, 조직감을 측정하였다. 실험결과, 조사선량이 높을수록 부피 증가율, 수화 복원력은 대조구에 비해 유의적으로 증가하였다. 조직감 중 경도는 침지온도, 시간 및 조사선량이 증가할수록 감소하여, 건고사리를 불리기 위해 드는 시간이 단축되어 에너지 절감의 효과가 있는 것으로 파악되었다.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.32 no.7
• /
• pp.991-996
• /
• 2003

Determination of regional microbial contamination in the process of rice rolled in dried laver (Kimbab) and effects of gamma irradiation on the improvement of hygienic quality and shelf stability were investigated. Total aerobic bacterial distribution of raw materials of Kimbab were; 10$^{6}$ ∼10$^{7}$ CFU/g in dried laver, 10$^3$ CFU/g in cucumber and below 10 CFU/g in steamed rice, ham, fried egg, and salted radish. Total coliform bacteria were 10$^3$ CFU/g in dried laver and detected below detection limit (10 CFU/g) in other raw materials. And it was arithmetically calculated that the levels of total aerobic bacteria and coliform bacteria in Kimbab does not exceed 10$^{5}$ CFU/g and 10$^1$ CFU/g under the aseptic process, respectively. However, microbial contamination levels in just prepared Kimbab in a market were about 10$^{6}$ CFU/g of total aerobic and coliform bacteria. Therefore, it was considered that microbial contamination of Kimbab is mainly originated from environmental uptake during the preparation. The representative media for putrefying bacterial growth were steamed rice. Coliform bacteria were mainly increased in ham and fried egg during storage. The bacteria in dried laver were radio-resistant and survived at 3 kGy of gamma irradiation. Coliform bacteria on EMB agar plate were eliminated at the dose of 2 kGy. The sensory acceptability of 2 kGy irradiated Kimbab was stable and the Kimbab can be preserved for 24 hour at 15$^{\circ}C$. Therefore, it was considered that optimal irradiation dose for radicidation of Kimbab was 2 kGy.

• The Korean Journal of Mycology
• /
• v.16 no.1
• /
• pp.41-48
• /
• 1988

To investigate germination characteristics of soils on Fusarium species under different soil conditions, this study was carried out to test spore germination of 4 Fusarium species. Among 102 soil samples, spore germinations of Fusarium solani f. sp. pisi, F. oxysporum f. sp. cucumerium, F. oxysporum f. sp. raphani and F. coccophilum were under 50% in 98, 85, 82 and 83 soil samples, respectively. The highest spore germination of F. solani f. sp. pisi, F. oxysporum f. sp. cucumerium and F. coccophilum was obtained in 21 soil samples of pH 5.1-6.0, whereas F. oxysporum f. sp. raphani was in 33 soil samples of pH 4.1-5.0. The highest spore germination of F. solani f. sp. pisi, F. coccophilum and F. oxysporum f. sp. cucumerium was obtained in 4 soil samples holding soil moisture content of 41-60%, while F. oxysporum f. sp. raphani was in 22 soil samples of 21-30%. F. oxysporum f. sp. raphani only showed a direct correlation(r=0.29) between spore germination and soil moisture content. On the basis of each spore germination in cultivated and non-cultivated soils, there was significant difference(p=0.01) in 4 Fusarium species. F. solani f. sp. pisi only indicated significant difference(p=0.0l) between two contrasting-soils, cultivated and non-cultivated soils. The numbers of microbial population were higher in soils suppressive to spore germination than soils conducive to spore germination.

• Applied Biological Chemistry
• /
• v.41 no.6
• /
• pp.457-464
• /
• 1998

For investigating the effect of amount of applied fertilizer with pig and chicken manures in the field, the number of soil bacteria were counted on the full strength conventional nutrient broth (NB) medium and its 100-fold dilution (DNB) medium. From the control soil samples without organic amendments, the number of bacteria on DNB medium was 5 to 10 times higher than that on NB medium. However, population density on NB medium was higher than on DNB medium from the treated soil with 60 and 120 Mg/㏊ for 3 years. Most of isolates from DNB plates did not grow on the NB plates. There were only grown on the 100-fold dilution medium, so it was called as DNB organisms. The DNB organisms were occupied as dominant group over 60% fo isolates in control soil. However, their occupation rates were rapidly decreased in the treatment soil with pig and chicken manures above 60 and 120 Mg per ha. These DNB organisms (oligotrophs) were significantly low population desities in the treatment soil with organic materials.

• Korean Journal of Microbiology
• /
• v.38 no.2
• /
• pp.127-132
• /
• 2002

In this study, the bacterial communities distributed in sea water of the whitening areas of Gangjeong and Seongsan, Jeju-do have been analysed using the PCR amplification of 16S rRNA to obtain fundamental data and information on relationship of the whitening phenomenon and microbial ecosystem. In Gangjeong, diverse bacteria such as Alcanivorax, Paracoccus, Damselae, Pseudomonas, Rhodowlum, Silicibacter, Suiftobacter, and Roseobacter have been found, and Alcanivorax was the most abundant clone. The most abundant clone from Seongsan was Pseudomonas, of which Pseudomonas tolaasii and Pseudomonas mandeli were most abundantly occurred in the frequency of approx44% and 24%, respectively. Approx4% of the bacterial clones closest to Verrucomicrobiales and other unidentified clones were also fecund in Seongsan, suggesting there is a great discrepancy between bacterial communities from the whitening areas of Seongsan and Gangjeong. The mean temperature, chlorine concentration, pH, and dissolved oxygen (DO) of the sea water of Gangieong and Seongsan in August of 2001 (sampling period) was $27^{\circ}C$~$27.5^{\circ}C$, 30.24~30.60%, pH 8.23~8.36,7 .20~7.28 mg/ι, suggesting other environmental factors except far the factors mentioned above might result in difference of bacterial communities distributed in both areas.

• Korean Journal of Microbiology
• /
• v.40 no.3
• /
• pp.205-210
• /
• 2004

The change of bacterial communities during cyanobacterial bloom was analyzed by DGGE in Daechung Reservoir from July to October in 2003. The traditional morphological analysis showed that the genera of Microcystis, Chroococcus, Oscillatoria, and Phormidium were dominated. The most frequent band in the DGGE profile by 16S rDNA sequence analysis was identified as Microcystis flos-aquae and the cyanobacterial bloom was peaked on September 2. Oscillatoria spp. were also identified and Aphanizomenon flos-aquae dominated in the middle of August. Judging from the analysis of the digitalized DGGE profiles using the cluster analysis technique, the microbial community on September 2 was considerably different from others. Consequently, it seems that the gene fingerprinting method can give not only the similar results to the traditional morphological method but also additional information on the bacterial species and similarity among the examined microbial communities.