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Analysis of Microbial Communities During Cyanobacterial Bloom in Daechung Reservoir by DGGE  

Ko So-Ra (한국생명공학연구원 환경생명공학연구실)
Park Seong-Joo (대전대학교 미생물학과)
Ahn Chi-Yong (한국생명공학연구원 환경생명공학연구실)
Choi Aeran (한국생명공학연구원 환경생명공학연구실)
Lee Jung-Sook (한국생명공학연구원 계통보존실)
Kim Hee-Sik (한국생명공학연구원 환경생명공학연구실)
Yoon Byung-Dae (한국생명공학연구원 환경생명공학연구실)
Oh Hee-Mock (한국생명공학연구원 환경생명공학연구실)
Publication Information
Korean Journal of Microbiology / v.40, no.3, 2004 , pp. 205-210 More about this Journal
Abstract
The change of bacterial communities during cyanobacterial bloom was analyzed by DGGE in Daechung Reservoir from July to October in 2003. The traditional morphological analysis showed that the genera of Microcystis, Chroococcus, Oscillatoria, and Phormidium were dominated. The most frequent band in the DGGE profile by 16S rDNA sequence analysis was identified as Microcystis flos-aquae and the cyanobacterial bloom was peaked on September 2. Oscillatoria spp. were also identified and Aphanizomenon flos-aquae dominated in the middle of August. Judging from the analysis of the digitalized DGGE profiles using the cluster analysis technique, the microbial community on September 2 was considerably different from others. Consequently, it seems that the gene fingerprinting method can give not only the similar results to the traditional morphological method but also additional information on the bacterial species and similarity among the examined microbial communities.
Keywords
cyanobacteria; DGGE (denaturing gradient gel electrophoresis); 16S rDNA;
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