• Journal of Sericultural and Entomological Science
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• v.44 no.1
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• pp.13-18
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• 2002

The morphorogical characteristics of the Morus tiliaefolia growing wild in the Korean peninsula are as follows. The chromosome number of somatic cell is 2n=84,that is hexaploid. The shape of male inflorescence is a long cylinder shape of 3.6∼6.0 cm. The length of female inflorescence is 1.8 cm, red violet or violet and the weight of mulberry fruits is 1.39∼1.65 g. The winter bud is very big in comparison with the branch and the accessory bud does not exist. The leaf is big and lobed leaf or entire leaf, the density of lenticell is 9.42$\pm$1.29 ㎠. The length of leaf trichome is 236∼438 ㎛ and the distribution density is 16∼37/25 ㎟ and heigh density of leaf back. The trichome shape of the leaf surface is cylinder shaped, in the leaf back it is thin and long corn shaped.

• Journal of Plant Biotechnology
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• v.30 no.2
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• pp.161-165
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• 2003

The effect of NAA and Benzyladenine(BA) for determination times on the formation of adventitious shoots, roots trichmoes and calli in MS basal medium was investigated in leaf segments from ecotype 'Nosses' of Arabidopsis thalliana. Adventitious shoots, roots, trichomes and calli were formed fromed from leaf segments in a wede range of NAA and BA. The optimal combination of hormones for adventitious shoots formation, 20mg/L NAA for trichome formation, 100mg/L for callus formation. Inductive times for formation of adventitious shoots, roots, trichomes and calli were determined at 14, 4, 6 and 18 days respectively by periodical transfer of leaf segments from hormines containing media to hormone free medium.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.1
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• pp.85-92
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• 2008

An RIL population from a Shinpaldalkong2/GC83006 cross was employed to identify quantitative trait loci (QTL) associated with agronomic traits in soybean. The genetic map consisted of 127 loci which covered about 3,000cM and were assigned into 20 linkage groups. Phenotypic data were collected for the following traits; plant height, leaf area, flowering time, pubescence color, seed coat color and hilum color in 2005. Seed weight was evaluated using seeds collected in 2003 to 2005 at Suwon and in 2005 at Pyeongchang and Miryang sites. Three QTLs were associated with 100-seed weight in the combined analysis across three years. Among the three QTLs related to seed weight, all GC83006 alleles on LG O ($R^2\;=\;12.5$), LG A1 ($R^2\;=\;10.1$) and LG C2 ($R^2\;=\;11.5$) increased the seed weight. A QTL conditioning plant height was linked to markers including Satt134 (LG C2, $R^2\;=\;25.4$), and the GC83006 allele increased plant height at this QTL locus. For two QTLs related to leaf area, 1aM on LG M ($R^2\;=\;10.0$) and laL on LG L ($R^2\;=\;8.6$), the Shinpaldalkong2 alleles had positive effect to increase the leaf area. Satt134 on LG C2 ($R^2\;=\;41.0$) was associated with QTL for days to flowering. Satt134 (LG C2) showed a linkage to a gene for pubescence color. Satt363 (LG C2) and Satt354 (LG I) were linked to the hilum color gene, and Sat077 (LG D1a) was linked to the seed coat color. The QTL conditioning plant height was in the similar genomic location as the QTLs for days to flowering in this population, indicating pleiotropic effect of one gene or the tight linkage of several genes. These linked markers would be useful in marker assisted selection for these traits in a soybean breeding program.

• Applied Microscopy
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• v.36 no.1
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• pp.35-45
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• 2006

Plants of Vitex negundo are known to develop numerous trichomes throughout their body, where certain trichome types have been believed to be one of the plausible structures for the unique scents. In the current study. structural aspects of the trichomes have been examined in leaves and stems of Vitex negundo using TEM and SEM. Trichome types as well as structural changes that occurred in certain trichomes during secretion have been mainly focused. Three type of glandular trichomes and two types of non-glandular trichomes were developed in the epidermis of young and mature Vitex negundo plants. The glandular trichomes included the peltate type (Type 1), the capitate type (Type 2), and degraded capitate type (Type 3), whereas the non-glandular warty trichomes contained the multicellular (Types 4) and unicellular type (Type 5). Type 1 and 2 consisted of head and stalk cells, but their number and size were different. One secretory cavity was formed from the four head cells in the former, but only two head cells were involved in the latter. The cytoplasmic density in the head cell was quite high and in particular, sER and Golgi bodies were well developed. At initiation of their development, the cuticle layer of the head cells separated from the outer tangential wall to form a secretory cavity. Subsequently the cavity expanded acropetally and a large number of secretory vesicles continuously produced from the head cells until they filled the entire cavity. The cavity contained materials that would be soon discharged into intercellular spaces and/or into the air. The cavity began to decrease the volume by contracting at initial secretion but degrade rapidly within short time. It has been suggested that the mode of secretion in V. negundo is probably the eccrine secretion, since no break or rupture of the cavity has been observed during examination. Contrastingly Type 3 exhibited deterioration of the head cell at early stage. Type 4 was about $110{\sim}190{\mu}m$ long, consisting of $2{\sim}3$ cells, and distributed more in the adaxial epidermis compared to the abaxial surface. However, $20{\sim}30{\mu}m$ long Type 5 was extremely dense in both epidermis. Among several trichome types, Type 1 and 2 probably play an important role in discharging unique aromatic scents in plants of V. negundo.

• Korean Journal of Plant Taxonomy
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• v.47 no.3
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• pp.222-235
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• 2017

A comparative study of the leaf epidermal micromorphology in the tribe Neillieae (Neillia: 4 species, 4 varieties; Physocarpus: 5 species; Stephanandra: 2 species) was carried out using scanning electron microscopy in order to evaluate the taxonomic and systematic implications of these characteristics. The leaves of the genera Neillia and Stephanandra were hypostomatic, whereas those of P. monogynus, P. opulifolius were amphistomatic. The range of the size of the stomata is $12.02-34.39{\times}10.76-27.13{\mu}m$; the smallest was found in N. thyrsiflora (average $13.98{\times}12.43{\mu}m$; $L{\times}W$), while the largest was measured in N. gracilis (average $26.82{\times}20.67{\mu}m$; $L{\times}W$). Paracytic stomata complexes are only found in N. affinis, and the anomocytic type was most commonly found. The papillate epidermal cell type was only observed on the abaxial surfaces of P. insularis. Platelet epicuticular waxes were found on the adaxial surfaces of N. affinis and S. tanakae. Four types (unicellular non-glandular, two- to five-armed, stellate, and glandular) of trichomes were found on the leaves. Stellates were observed in all species of Physocarpus except for P. insularis. Consequently, leaf epidermal micromorphological characteristics (e.g., the presence of papillate epidermal cells and stellate, and stomata complexes) may have high taxonomic and systematic value in Neillieae. Our results strongly support previous molecular phylogenetic and palynological hypotheses that Stephanandra and Neillia are a single genus and that Physocarpus insularis should be considered as a member of Spiraea.

• Korean Journal of Environment and Ecology
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• v.26 no.2
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• pp.192-199
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• 2012

This study was to evaluate the application of microhistological analysis to collect information on food materials of korean water deer from september to October 2006. For microhistological analysis we extracted type-materials of 4 plant species ($Ipomoea$ $batatas$, $Rubus$ $coreanus$, $Glycine$ $max$, and $Pueraria$ $thunbergiana$) from fecal pellets of korean water deer ($Hydropotes$ $inermis$) and then made preparations of the fecal materials for microscopic analysis. The fecal preparations were compared with reference preparation under the microscope. In microscopic analysis of fecal preparations, we could identify the four plant species by the followings: tip shape and morphology of trichomes, epidermal cell, morphological composition and shape of stomatal apparatus, arrangement of long-cell, and distribution of crystals in cells. According to the comparisons of the preparations, the morphology of trichome, epidermal cell and stomata are similar between both preparations of the fecal pellets and the references, respectively. This methodology will contribute to understanding feeding behavior and behavioural ecology by climate change of other hervibores as well as korean water deers.

• Korean Journal of Plant Taxonomy
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• v.44 no.2
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• pp.119-131
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• 2014

A comparative study of leaf anatomy in tribe Sorbarieae (Adenostoma, Chamaebatiaria, Sorbaria, Spiraeanthus) including one related genus Lyonothamnus was carried out using light microscopy. Anatomical characteristics of the leaf blade and midrib were described and taxonomically evaluated. The anatomical characters which described in this study are as follows: thickness of leaf midrib, blade in cross section, cuticle, epidermal cell, stoma, trichome, mesophyll, crystal, main vasculature type. All features were compared and the vascular patterns of midrib were distinguished two types. - Type 1: Trace tripartite (Adenostoma), Type 2: Trace continuous, subtype 2A: flat arc (Chamaebatiaria, Spiraeanthus), subtype 2B: U-shape arc (Lyonothamnus, Sorbaria). In conclusion, some of leaf anatomical characters (e.g., cuticle, epidermal cell, trichome, mesophyll, main vasculature type) can be useful for diagnostic features. Hypostomatic type, dorsiventral mesophyll, Ushape vasculature type would constitute a major characters for genus Sorbaria in Sorbarieae. The detailed anatomical description of studied taxa is provided, and its systematic importance is also briefly discussed.

• Korean Journal of Plant Taxonomy
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• v.40 no.1
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• pp.50-58
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• 2010

The nutlet morphology and pericarp structure of 14 taxa (4 taxa of the genus Glechoma and related taxa) were studied by light and scanning electron microscopy, and a detailed description of nutlet morphological features for all examined taxa is provided. The shape of the nutlet is ovate to elliptic. Its size is 1.36-3.83 mm in length and 0.80-2.65 mm in width. The largest one was found in Marmoritis rotundifolia, while the smallest one was in Agastache nepetoides. Three different surface types were recognized in the studied taxa based on differences in cell outline and cell boundary relief (rectangular, pitted, and ridged negative reticulate-cell). Three types of trichome (uni-cellular non-glandular trichome, multi-cellular non-glandular trichome, and peltate glandular trichome) were distributed in the nutlets. The pericarp is formed in three layers (i.e., exocarp, mesocarp, and endocarp including sclenchyma tissue), and the pericarp in the studied species is $39.0-237.5{\mu}m$ thick. The morphological and anatomical features (the nutlet shape, surface type, and trichome distribution) in the genus Glechoma and related taxa are described and discussed.

• Korean Journal of Plant Resources
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• v.12 no.1
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• pp.50-61
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• 1999

The leaf morphological and anatomical characters about 17 taxa of Aster and its allied taxa were investigated to estimate taxonomic values. Leaf shapes of the treated taxa were divided into five types; elliptic, spathulate, lanceolate, linear, ovatodeltoid. These types were fixed in same taxa, but variable among different taxa, therefore useful as taxonomic character. Leaf margins were divided into four types; entire, serrate, dentate, incised, these types were invariable in most taxa, but variable among individual in same taxa such as Kalimeris incise, Aster spathulifolius. The size and shape of leaf epidermal cell, the size and distributional numbers per 10$\textrm{mm}^2$ of stomata, deposit feature of cuticle were not distinguished clearly from treated taxa, but presence of stomata on adaxial surface, size and distributional numbers per 10$\textrm{mm}^2$ of stomata were useful taxonomic characters in some taxa such as Gymnaster koraiensis, Aster altaicus var. uchiyamae, Aster tripolium, Heteropappus arenarius. The leaf trichomes were divided into five types by basic form, sculpturing on their outer surface and cell arrangement; uniseriate granulate conical type, uniseriate psilate conical type, uniseriate psilate filiform type, globular type, biseriate vesicular capitate type. In spite of various habitat, basic morphology of trichomes were not changed, therefore, it was thought to be good taxonomic character.

• Korean Journal of Medicinal Crop Science
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• v.1 no.2
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• pp.171-177
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• 1993

This experiment was conducted to select and rear the adaptable cultivar which isused for various purpose and to build a year-round cultivation using with characters of aerial part and analysis of chemical components in 28 local cultivar of Artemisia princeps Var. The growth of aerial part, which are stem length, stem diameter, no. of branch, node, leaf size and leaf pubescence, in north local cultivars was more vigorous than those of south local caltivars. Fresh weight of "Myeongcheonssuk " was highest in local caltivars, and in view of local area, local cultivars which was collected from Iri Si and Euiseong Gun was shown the highest fresh weight. Local cultivars as mentioned above had a characters with plenty of leaves, nodes and pubescence of leaf is long. In chemical components of leaves in morth cultivar, content of N, MgO, Zn, Cu, Mn was high, while $P_2O_5,\;K_2O$ in middle local cultivars, Fe in south local cultivars. The more content of carbohydrate in leaves was high, the content of $K_{2}O$ had a increasing tendency. In local cultivars which was collected form Iri Si and Euiseong Gun with the highest fresh weight, content of $N,\;P_{2}O_5,\;K_{2}O$ was high, on the other hand content of MgO, Zn, Ca, Mn, Fe, was low in leaves of Artemisia Princeps Var.