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http://dx.doi.org/10.5010/JPB.2003.30.2.161

Determination Times for Induction of Adventitious Shoots, Roots, Trichomes, and Calli from Segments of Arabidopsis thaliana by NAA and BA  

Kim, Song-Lim (Division of Life Science, Hallym University)
Han, Tae-Jin (Division of Life Science, Hallym University)
Publication Information
Journal of Plant Biotechnology / v.30, no.2, 2003 , pp. 161-165 More about this Journal
Abstract
The effect of NAA and Benzyladenine(BA) for determination times on the formation of adventitious shoots, roots trichmoes and calli in MS basal medium was investigated in leaf segments from ecotype 'Nosses' of Arabidopsis thalliana. Adventitious shoots, roots, trichomes and calli were formed fromed from leaf segments in a wede range of NAA and BA. The optimal combination of hormones for adventitious shoots formation, 20mg/L NAA for trichome formation, 100mg/L for callus formation. Inductive times for formation of adventitious shoots, roots, trichomes and calli were determined at 14, 4, 6 and 18 days respectively by periodical transfer of leaf segments from hormines containing media to hormone free medium.
Keywords
Adventitious shoots; adventitious root; trichome; callus; NAA; BA;
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1 Christianson ML, Warnick DA (1985) Temporal requirement for phytohormone balance in the control of organogenesis in vitro. Dev Biol 112: 494-497   DOI
2 Conner TW, Goekjian VH, LaFaytte PR, Key JL (1990) Structure and expression of two auxin-inducible genes from Arabidopsis. Plant Mol Biol 15: 623-632   DOI   ScienceOn
3 Feldman JH, Marks, MD (1986) Rapid and efficient regeneration of plant of Arabidopsis thaliana. Plant Sci 47: 63-69   DOI   ScienceOn
4 Han TJ (1994) Change in specific protein profiles during initiation of adventitous roots in soybean (Glycine max L) cotyledon. Kor J Plant Tiss Cult 21: 123-129
5 Skoog F, Miller CO (1957) Chemical regulation of growth and organ formation in plant tissue cultured in vitro. Symp Soc Exp BioI 11: 118-140
6 Walker KA, Wendeln ML, Jaworski EG (1979) Organogenesis in callus tissue of Medicago sativa The temporal seperation of in induction process from differentiation processes. Plant Sci Lett 16: 23-30   DOI   ScienceOn
7 Chaudhury MA, Signer ER (1989) Relative regeneration proficiency of Arabidopsis thaliana ecotypes. Plant Cell Rep 8: 368-369   DOI   ScienceOn
8 Han TJ, Jeon MG, Jo HI (1996) Effect of polyamines on adventitious shoot formation from tobacco (Nicotiana tabaccum) leaf segments. Kor J Plant Tiss Cult 23: 45-50
9 Christianson ML, warnick DA (1983) Competence and determination in process in viitro shoot organogenesis. Dev BioI 95: 288-293   DOI   ScienceOn
10 Christianson ML, warnick DA (1984) Phenocritical times in the process of in vitro shoot organogenesis. Dev Biol 101: 382-390   DOI   ScienceOn
11 Han TJ, Kim IH, Kim SL, Kim JC, Jin CJ, Jin CD (1999) Organ formation-The formation of adventiticus roots, trichomes and calli from segements of Arabidopsis thaliana by Naphthaleneacetic acid concentrations, and their determination times. Kor J Plant Tiss Cult 26: 211-217
12 Jeon MG, Jo HI, Han TJ (1998) Effect of polyamines on adventitious root formation from tobacco (Nicotiana tabaccum) leaf segmenis. J Plant BioI 41: 31-36   DOI   ScienceOn
13 Michalzuk L, Ribnicky DM, Cooke TJ, Cohen JD (1992) Regulatirn of indole-3-acetic acid blosynthetic pathways in carrot cell cultures. Plant Physiol 100: 1346-1353   DOI   ScienceOn
14 Mok DWS, Mok MC (1994) Cytokinins: Chemistry, Activity and Function. CRC Press, Boca Raton, FL
15 Miller CO, Skoog F, Okamura FS, von Saltza MH, Strong FM (1956) Isolation, structure and synthesis of kinetin, a substance promoting cell division. J Am Chem Soc 78: 1345-1350
16 Miller CO, Skoog F, Von Saltza, MH Strong FM (1955) Kinetin, a cell division factor from deoxyribonucleic acid. J Am Crem Soc 77: 1292-1293   DOI