• Journal of Nutrition and Health
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• 제30권8호
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• pp.920-929
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• 1997

This study examined the effect of dietary salt levels on the incidence and cure of gastric ulcer in rats. Two sets of experiment were conducted . In the first experiment, the rats were divided into 3 groups. The 3 groups were fed 0%, 4%, and 8% NaCl diets respectively for 20days. The rats were given water -immersion restraint stress at the end of the dietary period , and sacrificed. The ulcer index by histological test was higher in rats fed the 8% NaCl diet than those in the other groups. The hexosamine and glutathione levels were significantly lower in the rats fed the 8% NaCl diet. Hematocrit and total iron binding capacity(TIBC) showed lower values caused by bleeding of gastric mucosa. The second experiment was designed to determine the effect of soldium concentration on the cure of gastric ulcer . As the gastric ulcer was recovered, ulcer length was gradually deceased in the control group but not changed in the 8% NaCl diet group. The gastric hexosamine and hepatic glutathione were increased in the control group but decreased in the 8% NaCl diet group. The hematologic indices of stressed rats showed the same tendency. As a result, dietary salt per se did not cause gastric ulcer . Once an ulcer is formed by stress or any other factor, higher levels of dietary salt may be detrimental to gastric mucosa, thereby delaying the healing of the ulcer. It is recommended that dietary salt intake be reduced in stress-prone people.

• KSBB Journal
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• 제29권1호
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• pp.42-49
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• 2014

The aim of this study was to develop a composite human skin equivalent for wound healing. Collagen type1 and acellular dermal matrix powder were utilized as the scaffold with dermal fibroblasts and keratinocytes for the development of a composite human skin equivalent. Fibroblast maintained the volume of composite skin equivalent and also induced keratinocytes to attach and proliferate on the surface of composite skin equivalent. The composite human skin equivalent had a structure and curvature similar to those of real skin. Balb-C nu/nu mice were used for the evaluation of full-thickness wound healing effect of the composite human skin equivalent. Graft of composite skin equivalent on full-thickness wound promoted re-epithelialization and granulation tissue formation at 9 days. Given the average wound-healing time (14 days), the wound in the developed composite skin equivalent healed quickly. The overall results indicated that this three-dimensional composite human skin equivalent can be used to effectively enhance wound healing.

• 구강회복응용과학지
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• 제28권2호
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• pp.171-178
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• 2012

보툴리눔 독소 주사는 이마, 눈가 주름치료 등 미용성형분야 뿐만 아니라 구강 악안면 분야에서는 만성 편두통(chronic migraine), 근육긴장이상(dystonia), 경직(spasticity), 측두하악장애(temporomandibular disorders, TMD)의 치료 등에 사용되어 왔다. 최근 보툴리눔 독소 주사가 운동신경에서 마비효과 뿐만 아니라 감각신경에서 말초감작과 신경원성염증과 관련되는 substance P, CGRP, glutamate 등 비콜린성 신경전달물질의 유리를 차단하여 통증신호를 차단하는 역할을 한다는 가설이 제기되고 있다. 따라서 본 연구는 쥐, 토끼 등 동물 신경 손상 모델에 대한 실험과 치과 치료 후 발생한 신경 손상 환자에서 보툴리눔 독소의 진통 효과 및 작용 기전을 살펴보고자 한다.

• 사상체질의학회지
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• 제19권2호
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• pp.155-169
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• 2007

1. Objectives This study aimed to investigate the anti-oxidative effect of Nocyongdaebo-tang (NYD) decoction in oxidized rats induced by AAPH. 2. Methods AAPH (2.2-azobis-2 aminodinopropane-hydrochloride) was injected intraperitoneally to induce oxidization and NYD was orally administered to the rats. To observe the anti-oxidative effect of NYD, we performed blood chemistry analysis, histological analysis, and evaluated the levels of SOD, catalase, glutathione, NO and MDA in liver. 3. Results & Conclusions 1) Serum Albumin level was increased significantly in the NYD group as compared with the control group and the saline group. 2) Serum LDL-cholesterol level was reduced significantly in the NYD group as compared with the control group. 3) GPT level in liver was reduced significantly in the NYD group as compared with the control group. 4) SOD, catalase activity and glutathione levels were increased significantly in the NYD group as compared with the control group and the saline group. 5) The levels of NO and MDA were reduced significantly in the NYD group as compared with the control group. 6) The degenerative change of liver tissue of NYD group was decreased in comparison with the control group. In conclusion, NYD decoction is considered to have an anti-oxidative effect in rats.

• 혜화의학회지
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• 제26권1호
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• pp.19-24
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• 2017

Objectives:Spring water is widely known to relax muscles by promoting blood circulation. This study was conducted to analyze theantithrombotic effect of naturally carbonated hot spring water (NCHW) to assess its influence on blood circulation. Methods:Atopic dermatitis was induced in the skin of the mice used in this experiment. NCHW was applied, and the antithrombotic effect was assessed and compared with that of other interventions. The positive control group was treated orally with aspirin. Results:After 3 weeks of exposure to NCHW, the experimental groupshowed a significant antithrombotic effect. NCHW also produced inhibitory responses to both collagen- and ADP-induced platelet aggregation, whereas the group given aspirin reacted only to collagen-induced platelet aggregation. Conclusions:The experiment demonstrated the intrinsic antithrombotic effects of NCHW compared with those of artificially carbonated water, tap water, and aspirin. This result suggests the possibility that NCHW can be used as a supportive and alternative treatment for vascular diseases.

• The Korean Journal of Pain
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• 제20권1호
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• pp.8-14
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• 2007

Background: Anticonvulsants and antidepressants are adjuvant analgesic drugs that are used widely for treating chronic neuropathic pain syndromes. The combined analgesic effect of gabapentin and milnacipran was investigated with a rat neuropathic pain model. Methods: The rat neuropathic pain model was made by ligating the spinal nerves (L5 and L6). An intrathecal catheter was inserted into the subarachnoid space. Tactile allodynia was tested with the up-down method using von Frey hair. We determined the antiallodynic effect of intraperitoneal (I.P.) and intrathecal (I.T.) gabapentin. The combined effect of I.P. gabapentin (50 mg/kg) and milnacipran (0, 10 and 30 mg/kg) was investigated. Results: Intraperitoneal and intrathecal administration of gabapentin increased the threshold for tactile allodynia (the ED50 was 60.6 mg/kg and $45.5{\mu}g$, respectively). Co-administration of I.P. milnacipran increased the antiallodynic effect of I.P. gabapentin in a dose-dependent fashion. Conclusion: The combined administration of milnacipran and gabapentin may increase the total analgesic effect during treatment of neuropathic pain.

• 대한방사선기술학회지:방사선기술과학
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• 제44권4호
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• pp.315-325
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• 2021

The purpose of this study is to investigate the changes in lipid proton (LP) composition according to the induced obese fatty liver and to use it as basic data for treatment and diagnosis of fatty liver in the future. The phantom study was conducted to identify differences between STEAM and PRESS Pulse sequences in LP concentration. A high-fat diet (60%) was administered to eight Sprague-Dawley rats to induce obesity and fatty liver disease. Baseline magnetic resonance imaging /spectroscopy data were obtained prior to the introduction of high-fat diet, and data acquisition experiments were performed after eight weeks using procedures identical to those used for baseline studies. The six lipid proton metabolites were calculated using LCModel software. The correlation between the fat percentage and each LP, revealed that the methylene protons at 1.3 ppm showed the highest positive correlation. The α-methylene protons to carboxyl and diallylic protons showed negative correlation with fat percentage. The methylene proton showed the highest increase in the LP; however, it constituted only 71.86% of the total LP concentration. The methylene proton plays a leading role in fat accumulation in liver parenchyma.

• 한국인터넷방송통신학회논문지
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• 제14권6호
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• pp.221-227
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• 2014

본 연구에서는 사용자의 위치정보를 제공하는 GPS와 카메라가 탑재된 원격 로봇과 스마트폰 어플 기술을 응용하여 화재 발생, 원전 사고, HF 가스 누출등 과 같은 급박한 화재 상황에서 소방관을 투입하기 이전에 정확한 화재 및 가스 누출 사고 현장의 정보와 상황을 파악하여 인명 피해를 최소화 하면서 방재 활동을 실시 할 수 있도록 하고 정확한 초기 대응으로 피해를 최소화 할 수 있는 전략을 수립 할 수 있도록 하는 소형화된 원격 로봇을 설계 제작하였다. 원격로봇은 카 보디와 콘트롤러 디바이스로 구성된다. 컨트롤 디바이스의 쥐는 강도에 따라 grab의 단계를 10단계까지 변경하는 것이 가능하였다. 초기 설계모델 로봇의 유용성에 대해 확인 하였다.

• 감성과학
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• 제8권2호
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• pp.155-160
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• 2005

본 연구는 관절통 모델에서 웅담 우황과 웅담 우황 사향의 약침액의 효과를 검사하기 위해 수행되었다. 할로탄 마취하에서 관절통은 수컷 쥐의 관절강내에 $2\%$ carrageenan을 주입하여 유발시켰다. 운동자극에 대한 감각신경의 반응은 약침을 시술하기 전과 후에 기록하였다. 경혈에 주입한 약침은 유해한 운동 자극에 대한 신경의 반응을 억제시켰다 족삼리에 시술한 약침은 합곡에 비해 유해한 자극에 대한 관절 감각신경의 반응을 더 많이 억제시켰다. 이러한 결과는 웅담 우황과 웅담 우황 사향의약침이 관절통을 완화하는 데 효과적인 치료법을 제공할 수 있다는 것을 시사한다.

• 한국식품과학회지
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• 제41권1호
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• pp.77-81
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• 2009

Streptozotocin-유도 당뇨병 모델을 이용하여 흑삼의 혈당강하작용에 대한 효과를 연구하고 증포과정에 따른 ginsenoside의 조성 변화를 분석하였다. 증포별 ginsenoside의 정량분석 결과, 증포 횟수가 증가할수록 조사포닌의 총량과 대부분의 ginsenoside는 줄어든 반면 ginsenoside-$Rg_3$ 함량 및 PD/PT 비율은 증가하는 경향을 보였는데, 이러한 조성은 다른 인삼제품과는 차별성 있는 특징이다. 또한, streptozotocin-당뇨병 유발쥐를 이용한 모델실험에서 흑삼추출물의 투여는 고혈당 준위(300 mg/dL이상)를 정상 수준(102 mg/dL)으로 감소시켰으며, 혈당 수준의 감소는 glycolysis나 HMT shunt 및 지방산 합성에 관여하는 glucokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, acetyl CoA carboxylase 등의 효소의 활성증대에 기인하는 것으로 판단되었다. 이러한 결과는 흑삼추출물의 유의적인 혈당강하 효과를 입증하며 이에 따라 신약이나 식이보조제의 항당뇨물질로서의 이용가능성을 시사한다.