• Title/Summary/Keyword: 멸치

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Purification and Biochemical Characteristics of a 45 kDa Fibrinolytic Enzyme from a Halophile (호염성균 유래 45 kDa 혈전용해효소의 순수분리와 생화학적 특성)

  • Kim, Do-Hyoung;Park, Jeong-Uck;Seo, Min-Jeong;Kim, Min-Jeong;Lee, Hye-Hyeon;Choi, Yung-Hyun;Joo, Woo-Hong;Jeong, Yong-Kee
    • Journal of Life Science
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    • v.20 no.2
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    • pp.183-189
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    • 2010
  • A fibrinolytic enzyme producing Bacillus sp. J-19 was isolated from the popular Korean seasoning, pickled anchovy. The fibrinolytic enzyme was purified to homogeneity by chromatographic methods including ethanol precipitation and gel-filtration using Sephadex G-50. Compared to the crude enzyme extract, the specific activity of the enzyme increased 1021-fold with a recovery of 23%. The purified enzyme was estimated to be approximately 45 kDa by SDS-PAGE. Especially, the amidolytic activity in the presence of the synthetic substrate for serine protease (H-D-Ile-Pro-Arg-pNA, S-2288) represented approximately 17 U/mg. In addition, more than the 60% activity of the 45 kDa fibrinolytic activity was maintained in the presence of up to 30% (w/v) sodium chloride. These findings could provide a unique fibrinolytic enzyme, leading to a potential thrombolytic agent.

A Study of Famous Traditional Kimchi in Pusan and Near Pusan Area (부산 및 부산근교의 명가김치 발굴을 위한 연구)

  • 문갑순;송영선;전영수
    • Korean journal of food and cookery science
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    • v.12 no.1
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    • pp.74-81
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    • 1996
  • Chinese cabbage Kimchi is a traditional fermented food and a numerous variety of Kimchies are produced in Korea. Most of Kimchis are produced on a small scale in the home for individual household use and recipes of Kimchis are deeply embedded in the individual cultures. Currently, however, factory-produced Kimchi is becoming popular by industrialization and changes of life style. This study was conducted to find out recipes of traditional Kimchis which are known delicious in Pusan and its environs and to develop a Kimchi with high quality. The characteristics of Kimchi recipes surveyed in Pusan and its environs are as follow: 1) Large amount of red pepper, garlic and anchovy sauce was used, which made Kimchi hot and spicy. 2) Extracts of dried anchovy or shrimp, or pear and onion juice was used to mix red pepper power. 3) Selection of good quality of Chinese cabbage and red pepper was very important step to determine quality of Kimchi. Furthermore, a numerous variety of submaterials added in Kimchi may also contribute to the high quality of Kimchi. 4) Salt concentration of Kimchi was around 2%, which was lower than it was generally known. Through this survey, it was suggested that maintaining low salt content of Chinese cabbage during salting make Kimchi crispy and juicy.

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A Study on Preference to Korean Spicy Fish Soups by Questionnaire Methods in Busan and Development of a Standardized Recipe (부산지역 생선횟집을 중심으로 생선 매운탕 소비실태 및 최적 조리법 확립)

  • Kim, Jung-Sun;Cho, Young-Je;Lee, Nahm-Gull
    • Journal of Fisheries and Marine Sciences Education
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    • v.18 no.2
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    • pp.150-163
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    • 2006
  • A study on sliced raw fish consumption was conducted on 300 citizens of Busan (89 men and 192 women), attending high school and university, housekeepers and employees. A self-administered questionnaire was used to record the results. The results were as follows: 93 percent of the respondents appeared to like or not be reluctant to eating Maeun-Tang. Most of the panelists had eaten at a seafood restaurant. The taste of Maeun-Tang varied differently by the spicy fish soup recipe. 56 percent of the respondents appeared to want this dish to have a fiery taste. Therefore, 89 percent of the respondents appeared to desire Maun-Tang to have a standardized recipe. The qualities of four kinds of stock made of water (A recipe), anchovy added with sea tangle (B recipe), fish bone (C recipe) and vegetable (D recipe) were investigated by using sensory evaluation and instrumental analysis for amino acids and nucleotides. The C recipe score was 80.9${\pm}$15.9 and D, B recipe was $75.7{\pm}17.1$, $75.4{\pm}17.2$, respectively. The A recipe score was $61.8{\pm}22.8$. The Degree of smooth taste were D recipe >A recipe >B recipe>C recipe. The Degree of spicy taste were C recipe >B recipe >D recipe>A recipe. In sensory evaluation, the C recipe obtained the highest score for overall preference. However, no difference of extractive nitrogen content, nucleotide and amino acid contest were observed in the C recipe after cook of Maeun-Tang.

The Opening Efficiency of half size modified net for the Anchovy Boat Seine (기선권현망 축소형 개량어구의 전개성능)

  • An, Young-Su;Jang, Choong-Sik;Lee, Ju-Hee
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.40 no.4
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    • pp.255-267
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    • 2004
  • This study was conducted in order to improvement of fishing gear and fishing operating system for anchovy boat seine by bag net A-type and B-type was attached with half size modified nets. Field experiments were carried out observe geometry of nets by catcher boats. The obtained results are summarized as follows : The actual ratio of net opening in each part of the half size modified nets was increased from fore part to back part of the nets as shown the smallest value about 20% in wing net and the biggest value about 110% in bag net. In addition, vertical net opening of B-type net as measured as 10.9~11.8m in fore bag net and 5.8~8.0m in after bag net were 1~2m greater than 9.0~13.6m of A-type one, and 2~3m less than 9.3~10.4m of A-type one, respectively. Vertical net opening of half size modified net was shown as less variation of towing depth from wing net to inside wing net than those of traditional nets due to stable vertical performance. The mesh distortion or drift and variation of vertical net opening were decreased by improvement of bag nets in order to minimized shape of net pocket phenomenon whenever towing speed is slow. Bag net B-Type attached with dual flapper was shown as less variation in width of bag net and less escapement of anchovy.

Fluctuation of the Catch by the Set Net Fishery (정치망어업의 어획량변동에 관한 연구)

  • 장호영
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.23 no.4
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    • pp.177-183
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    • 1987
  • The catch by the set net fishery from 1971 to 1985 were analyzed in order to reveal its fluctuation characteristics. Generally, the catch increased from 16,787M/T in 1971 to 84,103M/T in 1985 and showed maximum in 1979 with 99,475M/T of catch. This trend was mainly related to the higher production of file fish which accounts for 64% of the total catch in 1979, and the change of catch statistical classification according to the revising of fishery law in 1976. It was also closely related to the variation of the sea water temperature in coastal area. The main fishing period of the set net fishery appeared from October to November. Catch of the set net fishery was led by that in the southern coast before 1976 and by that in the eastern coast from 1977 to 1980, the tendency changed irregular from 1981. Three groups of fishes could be distinguished by principal component analysis of the annual catch. A group showing steady decreasing tendency was composed of the fishes such as whiting, sand fish, hair tail and anchovy. The another group composed of small alaska pollack, round herring, goby, gizzard shad, sand lance and file fish revaled an increasing tendency. The other fishes did not show any annual tendency.

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A Study on Major Local Foods in Gwangju.Jeonam Area (광주.전남지역의 주요 특산식품에 대한 연구)

  • Kim, Jeong-Ok;Choi, Cha-Ran;Shin, Mal-Shick
    • Korean Journal of Human Ecology
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    • v.15 no.2
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    • pp.327-339
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    • 2006
  • We have investigated the distribution of major local foods cultivated in Gwangju City and Jeollanam-Do in order to understand the food culture of Honam region. The local foods were divided into 4 classes: agricultural, aquatic, animal and other products. 124 species of local foods, including 51 agricultural products, 50 aquatic products, 6 animal products and 17 others, were identified from this region. In Jeollanam-Do area, the agricultural products have a variety of fruits and vegetables, and aquatic products have fishes. In particular, rice, cucumber, pear, anchovy, oyster, laver, oyster mushroom, shiitake mushroom, and honey were determined as major local foods in Jeollanam-Do area. Note that Mudeungsan watermelon is cultivated in Gwangju, and sweet smelt and cat fish caught only in Sumjin river and Tamjin river area. In recent years, pumpkin (Cucurbita maxima Duchesne), paprika, kiwi and melon were determined as major local foods in Jeollanam-Do. Furthermore, branding strategy of agricultural and animal products can be said to improve. Wild types of aquatic productions, mushrooms and wild edible greens have been decreasing, whereas cultivated production increasing. The kinds of special local foods in Gwangju City and Jeollanamdo area were influenced by geographical, religious and social factors.

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Processing of Functional Enzyme-hydrolyzed Sauce from Anchovy Sauce and Soy Sauce Processing By-products 1. Optimization of Hydrolysis Conditions by Response Surface Methodology (멸치액젓 및 간장 가공부산물을 이용한 기능성 효소분해간장의 제조 1. 반응표면분석법에 의한 가수분해물 제조조건의 최적화)

  • Kim, Hun;Lee, Jung-Suck;Cha, Yong-Jun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.4
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    • pp.653-657
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    • 2002
  • The hydrolysis conditions (enzym $e_strate ratio, time and temperature) of the mixture of anchovy sauce residue (ASR) and soy sauce residue (SSR) after fermentation by Flavourzyme 500M $G^{TM}$ were optimized using response surface methodology (RSM) for pretreatment of processing functional enzyme-hydrolyzed sauce. A model equation obtained from RSM was hydrolysis ratio (%) = 28.157+1.929enzym $e_strate ratio+1.818time+2.038temperature-1.093temperatur $e^2$, whose stationary point showed saddle point. From the ridge analysis of the saddle point, the conditions producing the highest hydrolysis ratio was determined as follows: 0.49% enzym $e_strate ratio; 3.55hr hydrolysis time; 62.5$^{\circ}C$ hydrolysis temperature. Adding of SSR to the mixture of water and ASR improved sensory qualities of mixture, so it seemed that SSR has masking effects on off-flavor and taste of ASR.R.of ASR.R.

A Survey on the Sensory Preference for Making Summer Kimchi by Nationwide Region (여름철 배추김치 담금시 지역별 관능적 선호도 조사)

  • Cha, Yong-Jun;Kim, Hun;Cho, Wo-Jin;Jung, Yeon-Jung;Lee, Young-Mi;Kim, Eun-Jeong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.3
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    • pp.393-399
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    • 2003
  • The purpose of this study was to investigate the preferred methods for making summer kimchi as a basic research of making commercial kimchi. Questionnaire were collected from 590 housewives nationwide in Korea, and the data were analyzed by SPSS program. The results were as follows: (1) The average salting time of Chinese cabbage was 3~5 hrs when the combined method of dry and brine salting was used, regardless of the region. (2) Seven jeotkals (salt-fermented fishes) including anchovy, anchovy juice, shrimp, northern sand lance juice, hair-tail viscera, flatfish and yellow corvenia were mainly used in kimchi. Among them, anchovy and anchovy juice jeotkals were preferred to all others in Southern area (Busan, Gwangju, Gyeongnam, Gyeongbuk, Jeonnam), while shrimp jeotkal in Seoul, Gwangju, Jeonbuk, Chungnam and Jeju, and northern sand lance juice jeotkal in Daegu, Daejeon and Gyeonggi, respectively. In most regions, however, blending type of 2 jeotkals was used in kimchi. (3) Eleven ingredients such as red pepper, garlic, ginger, green onion, radish, leek, onion, carrot, sugar, sesame and MSG were used as basic components for making kimchi. In particular, MSG was used as a basic ingredient regardless of region and age. However, a standard taste for making kimchi was depended on housewife in this study.

Effects of Ethanol and Organic Acids on Color, Fishy Odor and in vitro Absorption Rate of Calcium of Dried Large Anchovy (자건대멸의 색택, 어취 및 in vitro 칼슘 흡수율에 미치는 주정과 유기산 처리의 효과)

  • Jo, Jin-Ho;Jang, Hae-Jin;Cho, Seung-Mock;Lee, Yang-Bong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.9
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    • pp.1471-1476
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    • 2005
  • The optimization of ethanol treatment was carried out by response surface methodology (RSM) which was expressed through change of ${\Delta}$E value for improvement of color of dried large anchovy. The optimum condition was shown as treatment with 7 volumes (v/m) of ethanol at $50^{\circ}C$ for 9 hrs. At this condition, the removal rates of trimethylamine (TMA) and fat considered as fishy odor-causing materials were 81.1 and $77.4\%$, respectively, when analyzed by solid phase microextraction (SPME)/gas chromatography and soxhlet method, respectively The effect of citric acid on the removal rate of TMA was the highest one among organic acid treatments. The removal rate of TMA was affected greatly by the concentration of organic acid rather than the temperature and time of treatment. $73\%$ of TMA was removed by treatment of $1\%$ of citric acid at $20^{\circ}C$ for 20 min. Specially, above $90\%$ of TMA could be removed by the combination of alcohol and citric acid treatment. In vitro absorption rate of calcium was also increased to $12.3\%$ by the combination of alcohol and citric acid treatment compared with $2.9\%$ of control.

Studios on the Processing of Low Salt Fermented Sea Foods 6. Taste Compounds of Low Salt Fermented Anchovy and Yellow Corvenia (저식염 수산발효식품의 가공에 관한 연구 6. 저식염 멸치젓 및 조기젓의 정미성분)

  • CHA Yong-Jun;LEE Eung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.18 no.4
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    • pp.325-332
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    • 1985
  • As a series of study for processing low salt fermented fish, this work was undertaken to investigate taste compounds of low sodium salt fermented anchovy and yellow corvenia comparing with conventional fermented fish ($20\%$ of salt contents) during the fermentation of 120 days at $25{\pm}3^{\circ}C$. The major amino acids in fermented anchovy at 60 day fermentation were lysine, alanine, leucine, valine, isoleucine, histidine, threonine and glycine, while those in fermented yellow corvenia at 90 day fermentation were lysine, leucine, alanine, valine, threonine, isoleucine, glutamic acid and methionine. These amino acids held $57\%$ of the total extractive nitrogen content for fermented anchovy and $41\%$ for fermented yellow corvenia, respectively. It was supposed from the results that principal taste compounds both for fermented anchovy and fermented yellow corvenia were free amino acids, and that nucleotides and their related compounds as well as total creatinine also played an assistant role. And also there was little difference between taste compounds of low salt fermented fish and those of conventional fermented fish irrespective of fish species.

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