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Mosquito Prevalence and Flavivirus Infection Rates in Gangwon-do, Republic of Korea (2012~2017년 강원지역에서 채집된 모기의 계절적 발생소장과 플라비바이러스 감염률)

  • Chung, Se-Jin;Ko, Seuk-Hyun;Ko, Eun-Mi;Lim, Eun-Joo;Kim, Young-Su;Lee, Wook-Gyo;Lee, Dong-Kyu
    • Korean journal of applied entomology
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    • v.58 no.2
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    • pp.89-99
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    • 2019
  • In total, 654,362 adult mosquitoes were captured using black light traps in Gangwon-do Province of the Republic of Korea from 2012 to 2017. The collected mosquitoes were identified to the species level, placed in pools of up to 50 mosquitoes each, by species and date of collection, and screened for flaviviruses using a reverse transcription-polymerase chain reaction assay. A total of 276,224 adult mosquitoes were grouped in 7,721 pools for virus testing, and 68 flavivirus positive pools (0.9%) were detected. Flavivirus-positive products were confirmed by DNA sequencing. Japanese encephalitis viruses were detected in single pools collected from Chuncheon (2012, 2017: Culex pipiens, 2,728 and 1,111 mosquitoes, respectively), Hoengseong (2013: Culex orientalis, 19), and Gangneung (2017: C. pipiens, 724). All the Japanese encephalitis viruses detected were revealed as genotype V. Chaoyang viruses were detected in 63 pools of 5,055 Aedes vexans nipponii and a single pool of 585 C. pipiens collected in Gangwon-do Province from 2012 to 2017. Chuncheon was the region with the highest minimum infection rates (MIR, 0.32) and maximum likehood estimate (MLE, 0.33; confidence interval (CI) 95%, 0.23-0.46) of A. vexans nipponii for Chaoyang virus, followed by Hoengseong (MIR 0.30, MLE 0.30, CI 0.16-0.52) and Gangneung (MIR 0.21, MLE 0.21, CI 0.13-0.31). Monthly MIR and MLE values of A. vexans nipponii for Chaoyang virus were the highest in October (MIR 0.38, MLE 0.38, CI 0.07-1.25).

Parasitylenchus orthotomici sp. n. (Tylenchida: Allantonematidae) from Orthotomicus angulatus (Coleoptera: Scolytidae), with Note on Parasitism (소나무뿔나무좀(Orthotomicus angulatus) (딱정벌레목 : 나무좀과)에서 발견된 천적선충 신종과 기생율)

  • ;Harry K. Kaya;Patrick Shea
    • Korean journal of applied entomology
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    • v.28 no.1
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    • pp.1-3
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    • 1989
  • Parasitylenchus orthotomici sp. n. is described as a nematode parasite of Orthotomicus an¬gulatus in Korea. In a sample of 170 beetles randomly collected from Pinus densiflora S. et. Z, 51. 8% were parasitized by nematodes. The number of nematodes per bark beetle was ranged from 1 to 97. The parasitisms of F, generation were ranged from 43.2% to 52%. There was no positive tendency in nematode parasitism between sun-side and shade-side.

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Effects of Root-knot Nematode, Meliodogyne hapla, on Growth and Yield of Pepper and Tomato (당근혹선충이 고추와 토마토의 생육 및 수량에 미치는 영향)

  • Cho H.J.;Han S.C.
    • Korean journal of applied entomology
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    • v.22 no.1 s.54
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    • pp.15-20
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    • 1983
  • Effects of the root-knot nematode, Meloidogyne hapla, on the growth and yields of hot-pepper (var.: Cheongyong gochu) and tomato$(var.:\;Bogsu\;\#)$ were studied with inoculating 500, 2,500, 5,000, 7,500, 10,000 and 20,000 nematodes per plant in pots. Results were analyzed with comparing weight of fruits harvested throughout the season from both hot-pepper and tomato, and with mineral contents in dried stoots and roots of pepper plant 20 weeks after the inoculation. No significant difference was found on the plant growth at all levels of inoculation until the 8th week after the inoculation. However, the plant growth was significantly depressed from the 12th week in the pots inoculated with over 10,000 nematodes, and the yields were reduced by $16\%$ in hot-pepper and $14\%$ in tomato respectively when 10,000 nematodes were inoculated.

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One human case of natural infection by Heterophyopsis continua and three other species of intestinal trematodes (긴이형흡충과 여러 종의 장흡충에 혼합감염된 인체감염 1례)

  • 홍성종;정창근
    • Parasites, Hosts and Diseases
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    • v.34 no.1
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    • pp.87-90
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    • 1996
  • The third human case of natural infection by Heterophvopsis continuo in Korea was found in Chinju, Kyongsangnam-do. The case was a 53-year-old mn. He used to eat raw brackish and fresh water fish. After praziquantel treatment and purgation, H. continua were collected from the diarrheal stool together with Metononimw vokognwai, Heterophves nocens and Echinostona hokense. His clinical complaints were indigestion, epigastric discomport, poor appetite and fatigue. The complaints were considered rather due to heavy M. Wokoscwci infection.

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Canine Brucellosis in the Jindo (진돗개에서 발생한 부루셀라병)

  • 장지훈;이정치;이채용;김상기;이정길
    • Journal of Veterinary Clinics
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    • v.17 no.2
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    • pp.321-326
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    • 2000
  • 전라남도 진도군 내에서 사육되는 진돗개에서 발생한 유산의 일부가 Brucell canis 에 의하여 유발된 것임을 확인하였다. 이 연구에서는 먼저 1997년 7월에서 1998년 6월 사이에 발생한 진돗개의 유산을 설문조사로 확인하였던 바 발생률이 18.5%에 달했다. 이어서 설문조사에 포함된 지역에서 사육되는 진돗개를 대상으로 신시한 혈청학적 조사에서는 25%가 양성을 보였으며, 수캐의 양성률은 암캐의 거의 두 배에 달했다. 다음으로 균 분리와 치료시험을 실시하기 위하여 유산 병력을 기치고 있으면서 혈청검사에서 양성을 나타낸 암캐 다섯 마리를 현지에서 구입하였다. 그 중 세 마리는 안락사 시킨 후 부검하여 자궁을 검사하고 균 분리를 시도한 바 세 미리 모두에서 B. canis가 분리되었다. 그러나 육안적 및 조직학적 변화 는 현저하지 않았다. 나머지 두 마리는 minocycline과 streptolmycin으로 치료하였는데, 치료를 끝낸 후 8주에 실시한 혈청검사에서 음성을 보였다. 이 연구의 결과는 개 부루셀라병이 진돗개를 사육하는 농가에 경제적 손실을 초래하고 있음을 나타내준다. 아울러 대부분의 진돗개가 방사되고 있으며, 그렇게 사육된 개들이 전국적으로 팔려나가고 있기 때문에 진돗개가 개 부루셀리병의 전파원으로 작용할 가능성을 지니고 있음을 나타내준다 천연기념물인 진돗개의 보 호와 농가의 소득 그리고 공중위생학적인 면에서 진품개의 부루셀라병에 관한 연구는 지속적으로 수행되어야 할 것으로 사료된다.

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A study on the Feature Local Descriptor for Recognition of Pet's Nose-print (반려동물 비문 인식을 위한 특징점 지역 기술자 연구)

  • Kim, Hyung-O;Hong, Sang-Beom;Hong, Chang-Ho
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2018.10a
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    • pp.556-557
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    • 2018
  • About 350 shelters nationwide go through about 100,000 organic animals every year. If you are not adopted by the adoption candidate, you will be euthanized in just one out of every fourteen days after entering the shelter. Therefore, in order to prevent the occurrence of organic animals, it is necessary to register the companion animal easily and to register the inscription to manage the history. In this paper, we propose a local technician who can describe feature points in inscription images to develop recognition technology through inscription, which can distinguish companion animals such as human fingerprints.

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뱀장어 유어(Anguilla anguilla)에 있어서 말라카이트 그린의 잔류성

  • Bergwerff, Aldert A.;Kuiper, Raoul V.;Scherpenisse, Peter
    • Korean Aquaculture
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    • v.19 no.1
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    • pp.43-51
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    • 2007
  • 말라카이트그린(MG) 및 L-말라카이트그린(LMG)의 소멸은 뱀장어 Anguilla anguilla 유생에서 100일정도 걸린다. 실뱀장어(평균체중: 4.1 g)가 $23.0-26.5^{\circ}C$의 다양한 수온범위에 24시간동안 아주 낮은 농도(0.1 mg/l MG)에 약욕을 실시하였다. 처리한 후 실뱀장어는 말라카이트그린이 없는 깨끗한 물이 들어 있는 수조로 옮겼다. 뱀장어가 수용된 수조에는 순환여과 된 사육수를 공급하였다. 일정한 시간 간격으로 수집된 10마리의 뱀장어 및 사육수의 샘플은 high-performance liquid chromatography (HPLC)으로 분석하였다. 말라카이트그린의 가장 높은 농도는 $435{\pm}59\;{\mu}g/kg(mean{\pm}S.D.)$로서 처리시작 후 6시간에서 나타났다. L-말라카이트그린(LMG) 대사물의 농도는 비교적 높게 나타났으며 ($>100\;{\mu}g/kg$), 처리시작 후 6시간 및 처리종료 후 1500시 사이에서 안정적으로 나타났고 최대농도는 $831{\pm}231\;{\mu}g/kg$이었다. 비록 뱀장어는 체중이 증가하지는 않았지만 노출 후 24시간 후에도 L-말라카이트그린(LMG)은 여전히 전어체 kg당 $15{\pm}12\;{\mu}g/kg$ 함량이 존재하였다. 말라카이트 그린은 처리 이후 1920 및 2400시에서는 발견되지 않았다. 생물적 여과사육수와 순환여과시스템에서는 말라카이트그린이 발견되지 않았다. 본 연구에서는 부가적으로 빠르게 성장하여 11개월 된 뱀장어 두 마리를 샘플하여 분석에 사용하였다. 두 마리의 뱀장어는 실뱀장어기(0.3g)일 때 $0.15\;mg\;l^{-1}$ 농도의 말라카이트그린이 처리되었었다. 뱀장어의 근육조직에서는 지질 크로마토그래피-질량 분광광도계분석에 의해 말라카이트그린과 L-말라카이트그린이 측정 가능한 양으로 함유되지 않음을 확인할 수 있었다($<\;0.2\;{\mu}g\;kg^{-1}$).

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Effect of Water Temperature and Culture Density on Growth and Survival of Juvenile Turbot Scophthalmus maximus during Summer Season (하절기 사육수온 및 밀도가 터봇 Scophthalmus maximus 미성어의 성장과 생존에 미치는 영향)

  • Lee, Bae-Ik;Nam, Myung-Mo;Byun, Soon-Gyu;Kim, Yi-Cheong;Lee, Jong-Ha
    • Journal of Aquaculture
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    • v.21 no.4
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    • pp.265-271
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    • 2008
  • Upper temperature tolerance of the turbot Scophthalmus maxim us, one of the popular aquaculture species in European community and China, was evaluated in terms of survival and growth at $20^{\circ}C$, $23^{\circ}C$, $26^{\circ}C$, or $29^{\circ}C$. Best growth was achieved at temperature $20^{\circ}C$ in this experiments. The fish exposed to $20^{\circ}C$ or $23^{\circ}C$ were comparable in survival, condition factor and feed conversion efficiency reminiscent of the latter temperature to be agreeable for the fish. The temperature over $23^{\circ}C$ appeared to be the temperatures beyond the fish can tolerate. For instance, the fish exposed to 26 showed mortality of 60.9% by day 60; none of the fish exposed to $29^{\circ}C$ survived beyond day 7. Culture densities between 80 and $200\;fish/m^2$ did not influence the survival, growth, condition factor and specific growth rate of the fish. The final production of the culture density experiment was $10\;kg/m^2$ on average. These results imply that the location where water temperature remains lower than $25^{\circ}C$ during summer can be a candidate site for the turbot aquaculture.

Occurrence of Insect Pests and Natural Enemies in Pear Orchard with Hairy Vetch (헤어리베치 재배과원에서 해충과 천적의 발생)

  • Choi, Yong-Seok;Whang, In-Su;Park, Deog-Kee;Choe, Gwang-Ryul
    • The Korean Journal of Pesticide Science
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    • v.17 no.4
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    • pp.448-453
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    • 2013
  • We investigated the occurrence of major pests, 4 species of moths (Grapholita dimorpha Busck, Carposina sasakii Matsumura, Archips breviplicanus Walsingham, Adoxophyes orana Fischer von Roslerstamm), apple aphid (Aphis spiraecola Patch), two spotted spider mite (Tetranychus urticae Koch) and parasitoids in pear orchard with hairy vetch and cutting weeds in Yesan, Chungnam province during the flowering season and summer depression season of hairy vetch, in 2012 and 2013. The occurrence of 4 species of moths in hairy vetch plot and cutting weeds (major is finger grass, Digitaria sanguinalis, 80%) were little different but in case of apple aphid, the density in hairy vetch plot was just 95 aphids per stem at 20th June but low after then. The density of two spotted spider mite on pear leaf in hairy vetch plot was lower than in cutting weeds plot significantly. The number of parasitoids collected in hairy vetch plot during flowering season (May and June) were 398 and 798 in 2012 and 2013, respectively. The number of parasitoids collected in hairy vetch plot during summer depression season (July and August) were 114 and 172, respectively. But in cutting weeds plot, 9 and 22 in May and June, 8 and 5 in July and August. We didn't know either hairy vetch in pear orchard affected the occurrence of 4 species of moths, apple aphid and two spotted spider mite or not but was acted to be favorable for wasps, the natural enemies on the contrary. The improvement of chemical control systems through the selection of low chemicals to natural enemies with cover and green manure plants would turn effective pest management into a possibility.

Longevity and Life Table of the Foxglove Aphid (Aulacorthum solani K.) Adults on Lettuce (Lactuca sativa L.) (상추를 먹이로 공급할 때 싸리수염진딧물 성충의 수명과 생명표)

  • Lee, Sang-Guei;Kim, Hyeong-Hwan;Kim, Tae-Heung;Park, Gil-Jun;Kim, Kwang-Ho;Kim, Ji-Soo
    • Korean journal of applied entomology
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    • v.47 no.4
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    • pp.365-368
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    • 2008
  • Adult longevity and fecundity of the foxglove aphid, Aulacorthum solani Kaltenbach, were studied at $12.5{\sim}25^{\circ}C$ with $60{\sim}70%$ RH under 16L:8D and the results were put together to build a life table. The longevity of foxglove gradually increased with decreasing temperature below $25^{\circ}C$. Also total fecundity increased with decreasing temperature and highest fecundity was 74.1 nymphs per female at $15^{\circ}C$. However, daily fecundity increased with increasing temperatures up to $20^{\circ}C$ showing 2.9 nymphs per day and thereafter decreased. Net reproduction rate ($R_0$) was highest of 58.7 at $15^{\circ}C$. The intrinsic rate of increase per day ($r_m$) and the finite rate of increase per day (${\lambda}$) were highest of 0.27 and 1.32, respectively and the doubling time (Dt) was shortest of 2.52. the mean generation time (T) was 10.99 at $22.5^{\circ}C$.