Jeung Jae Yeal;Milton Donald K.;Kim Tae Hyeung;Lee Jong Young;Chong Myoung Soo;Ko Kwang Jae;Kim Sang Duck;Kang Sung Ho;Song Young Sun;Lee Ki Nam
Journal of Physiology & Pathology in Korean Medicine
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v.16
no.3
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pp.464-471
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2002
Author applied several engineering methodologies to classical ultrasonic nebulizer to cope with it's demerits. After several trials and errors, we got the several meaningful results. To evaluate the modified ultrasonic nebulizer for inhalation toxicology of cadmium, author used light-scattering photometer. This paper is the one part of inhalation exposure systems for inhalation toxicology study of cadmium. According to the testing conditions, source temperature 50℃ and inlet-duct band temperature 150℃, aerosol generation results for sodium chloride and cadmium chloride were as followings: Coefficients of variation(CV) of sodium chloride and cadmium chloride for repeated trials were 3.38 and 4.77 for 10g, 2.47 and 5.02 for 5g, and 4.70 and 2.98 for 2.5g. All the CVs were within 10% of acceptance variability. Count Per Minute(CPM) changes of NaCl and CdCl₂ for 5 repeated trials were similar. CPM ratios of CdCl₂/NaCl were 1.13 for 10g, 0.76 for 5g, and 1.06 for 2.5g. Relative aerosol generation of cadmium chloride to sodium chloride was the highest in 10g. Efficiency increases of 24.50% for 5g NaCl, 14.91 % for 2.5g NaCl, and 16.48% for 2.5g CdCl₂ with respect to theoretical efficiency were observed but 0.04% efficiency decrease was observed in 5g CdC₂. According to the modifications of source temperature(20, 50, 70℃) and inlet-duct band temperature(20, 50, 100, 150, 200℃), aerosol generation results for NaCl and CdCl₂ were as followings: CPM trends for each quantity excepting 10g NaCl in inlet-duct band temperature 200℃ were similar, and the highest CPM was observed in source temperature 70℃ to each inlet-duct band temperature. The highest CPMs to 10, 5, and 2.5g NaCl were observed in source temperature 70℃ and inlet-duct band temperature 20℃. Aerosol generation of cadmium chloride was increased with the higher source temperature, excepting inlet-duct band temperature 200℃. The highest CPMs for 10, 5, and 2.5g CdCl₂ were observed in source temperature 70℃ and inlet-duct band temperature 20℃, and this trend was similar to NaCl aerosol generation The highest CPMs for 10, 5, and 2.5g CdCl₂ were observed in source temperature 70℃ and inlet-duct band temperature 20℃, and this result was similar to NaCl aerosol generation. Observed efficiencies of 5 and 2.5g NaCl were similar to ifs theoretical efficiency but -3.08% efficiency decrease of 5g CdCl₂, 17.47% efficiency increase of 2.5g CdCl₂ were observed. CPM ratio of CdCl₂/NaCl of 10g was different to 5 and 2.5g, and 2.5g ratio was higher than 5g ratio. In conclusion, to get maximum aerosol generation for NaCl and CdCl₂ will be the conditions that set the appropriate inlet-duct band temperature for each materials and increase the source temperature. Sodium chloride can be used to evaluate the performance and predict the concentration for cadmium aerosol in aerosol generator and inhalation exposure system.
Jeung Jae Yeal;Kang Sung Ho;Kim Sam Tae;Lee Eun Kyoung;Song Young Sun;Lee Ki Nam
Journal of Physiology & Pathology in Korean Medicine
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v.17
no.2
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pp.518-524
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2003
Ultrasonic nebulizer with the application of new engineering methodology and the design of electronic circuit was made for lead inhalation toxicology study and 2730ppm lead nebulizing solution was used to generate lead aerosol. After modification of source and inlet temperatures, the results of particle size analysis for lead aerosol were as following. The highest particle counting for source temperature 20℃ was 39933.66 in inlet temperature 100℃ and particle diameter 0.75tLm. The highest particle counting for source temperature 50℃ was 39992.71 in inlet temperature 250℃ and particle diameter 0.75μm. The highest particle counting for source temperature 70℃ was 37569.55 in inlet temperature 50℃ and particle diameter 0.75μm. The ranges of geometric mean diameter(GMD) were 0.754-0.784μm for source temperature 2℃, 0.758-0.852μm for source temperature 50℃, and 0.869-1.060μm for source temperature 70℃. The smallest GMD was 0.754μm in source temperature 20℃ and inlet temperature 20℃, and the largest GMD was 1.060μm in source temperature 70℃ and inlet temperature 250℃. The ranges of geometric standard deviation(GSD) were 1.730-1.782 for source temperature 20℃, 1.734-1.894 for source temperature 50℃, and 1.921-2.148 for source temperature 70℃. The lowest GSD was 1.730 in source temperature 20℃ and inlet temperature 20℃, and the highest GSD was 2.148 in source temperature 70℃ and inlet temperature 250℃. Lead aerosol generated in this study was polydisperse. The ranges of mass median diameter(MMD) were 1.856-2.133μm for source temperature 20℃, 1.877-2.894μm for source temperature 50℃, and 3.120-6.109μm for source temperature 70℃. The smallest MMD was 1.856μm in source temperature 20℃ and inlet temperature 20℃, and the largest MMD was 6.109μm in source temperature 70℃ and inlet temperature 250℃. Slight increases for GMD, GSD, and MMD values were observed with same source temperature and increase of inlet temperature. MMD for inhalation toxicology testing in EPA guidance is less than 4μm. In this study, source temperature 20℃ and 50℃ with inlet temperature from 20℃ to 250℃ were conformed to the EPA guidance, but inlet temperature 20℃ and 50℃ for source temperature 70℃ were conformed EPA guidance. MMD for inhalation toxicology testing in OECD and EU is less than 3μm. In this study, source temperature 20℃ and 50℃ with inlet temperature from 20℃ to 250℃ were conformed to the EPA guidance, but none for source temperature 70℃.
Cheon, D.W.;Lee, S.Y.;Park, M.S.;Park, H.S.;Hwang, K.J.;Yun, S.H.;Ko, M.S.
Journal of The Korean Society of Grassland and Forage Science
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v.27
no.4
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pp.297-312
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2007
This study is conducted to evaluate economical value of Jeju grassland and validity of its preservation, and draw up several measures to support. To measure its economical value, this study examined its environmental value and social and cultural value. For environmental value, this study used replacement method while it applied CVM method, a widely used method, to assess social and cultural value and two-level two-best choice selection method, which ask questions by assuming virtual circumstances to avoid reflecting some biased opinions. Jeju grassland has multiple functions-environmental functions such as preventing soil corrosion and flood, handling animal excrement, and purifying air, and social and cultural functions such as promoting physical and mental health and providing recreation places. From the results of the feasibility study, Jeju grassland's annual multiple functions are assessed to have a total $397,115{\sim}418,995$ million won worth. In addition, it is found that Jeju visitors recognize Jeju grassland for its functions to contribute to public interests. That is, they think it can provide attractive views and educational and recreational places and promote emotional development. Especially, many people presented their ideas that it be continuously preserved since it is worthwhile for us and our next generations. To preserve grassland's cultural resource, which create a huge economic value like this, the Government has to support a certain amount of financial aid for turning to a better grassland environment and its maintenance to realize environment-friendly livestock farming on Jeju Island and promote its tourism industry and consequently, add more value to Jeju.
Nam, Cheol Hwan;Kim, Ki Soo;Park, Man Ho;Yun, An A;Park, Jong Ho;Han, Ouk Kyu;Kim, Won Ho;Sun, Sang Soo
Journal of The Korean Society of Grassland and Forage Science
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v.39
no.4
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pp.207-215
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2019
This study was conducted to establish spring sowing techniques in preparation for the impacts of climate change on sowing time and wintering rates of winter forage crops such as barley, oat and IRG. Oat showed the highest yield in 2017 which had relatively dry climate condition. And when sowing in late Febrnary 2017 yielding 9,408kg/ha were obtained, yielding 4,011kg/ha more than IRG's sown in the same period. In 2018 which had relatively wet climate condition, four barley species decreased in the production from the previous year. Oat also had decreased by 70% from 9,408kg/ha to 2,851kg/ha. On the other hand, IRG maintained the production in the mid-5,000kg/ha range. It was also found that IRG had the least variability due to external influences regardless of seeding period for 2 years. Mixed sowing with IRG and oat in 50:50 ratio was the highest dry matter, 6,584kg/ha, and IRG was 18.5% and Oat was 2.3 times higher than single planting.
Acrolein is a highly toxic, reactive mutagenic aldehyde. Humans are exposed to acrolein vapors from overheated cooking oil, cigarette smoke, and combustion of organic products. In this study, we investigated elementary and middle school students' consumption of foods cooked at high heat, such as fried, direct fire, and indirect fire cooked foods. The subjects of this survey study were 265 fifth grade students and 396 middle school students nationwide. According to the results of the frequency test, broiled fish was the most frequently consumed, followed by fried fish, indirect cooked samgyebsal, and other fried foods, which were eaten more than three times per month. The total intake of foods cooked at high heat per child over 1 year was in the following order: Fried chicken(7.50 kg) > Samgeybsal(6.57 kg) > Broiled pork ribs(4.18 kg) > Broiled fish(4.12 kg) > Bulgogi(3.31 kg) > Fried dish(3.18 kg) > Pork cuttlet(3.17 kg) > Jeyukbockeum(3.13 kg) > Charcoal-broiled beef ribs(2.74 kg) > Roasted whole chicken(2.05 kg) > Dackochi(1.87 kg) > Tangsuyuk(1.87 kg) > Fried fish(1.83 kg) > Hamburger patties(1.21 kg) > Fried potato(1.01 kg) > Barbecue(0.95 kg) > Doughnut(0.80 kg) > Corn dog(0.66 kg) > Croquette(0.51 kg). The favorite cooking method was indirect fire cooking for beef, pork, and fish, whereas frying was preferred for chicken. The results of this study provide important data for assessment of acrolein exposure via foods cooked at high heat temperature. The results also indicate that children might be exposed to acrolein through consumption of foods cooked at high heat temperature, suggesting the need to promote education of proper food consumption behavior.
The purpose of this study was to investigate the problems of a signal to noise ratio measurement using a two region measurement method that is conventionally used when using a multi-channel coil and a parallel imaging technique. As a research method, after calculating the standard SNR using a single channel head coil of which coil satisfies three preconditions when using a two region measurement method, we made comparisons and evaluations after calculating an SNR by using a two region measurement method of which method is problematic because it is used without considering the methods recommended by reputable organizations and the preconditions at the time of using a multi-channel coil and a parallel imaging technique. We found that a two region measurement method using a multi-channel coil and a parallel imaging technique shows the highest relative standard deviation, and thus shows a low degree of precision. In addition, we found out that the difference of SNR according to ROI location was very high, and thus a spatial noise distribution was not uniform. Also, 95% confidence interval through Blend-Altman plot is the widest, and thus the conformity degree with a two region measurement method using the standard single channel head coil is low. By directly comparing an AAPM method, which serves as a standard of a performance evaluation test of a magnetic resonance imaging device under the same image acquisition conditions, an NEMA method which can accurately determine the noise level in a signal region and the methods recommended by manufacturers of a magnetic resonance imaging device, there is a significance in that we quantitatively verified the inaccurate problems of a signal to noise ratio using a two region measurement method when using a multi-channel coil and a parallel imaging technique of which method does not satisfy the preconditions that researchers could overlook.
The purpose of this study is to measure scattered ray which is occurred except for Z-axis range of the detector in MDCT's iso-center and present the basic data about the standard for reduction of scattered ray. The development of MDCT brings out the enlargement of beam thickness to the patient's Z-axis, which distributes to the increase in exposure dose according to the rise of scattered ray. Also MDCT brings out the increase of scattered ray about 4times more than SDCT. To evaluate scattered ray according to the change of beam thickness on MDCT, we measured scattered ray of MDCT's Z-axis beam thickness by using one 16-slice CTs and two 64-slice CTs. We used the ionization chamber 60ml 2026C as the equipment of measurement. In our results, we found out that the change of scattered ray according to the beam thickness in the same kVp has increase of scattered ray. Secondly we found out the increase of scattered ray according to the increase of kVp. Lastly we found out the decrease of scattered ray according to the increase of the distance from the ionization chamber.
In Korea, little attention has been paid to microbial perchloroethylene (PCE) and/or trichloroethylene (TCE) dechlorination activity and identification of microorganisms involved in PCE reductive dechlorination at a PCE-contaminated aquifer. We performed microcosm tests using the groundwater samples from 4 different contaminated sites (i.e. Changwon A, Changwon B, Bucheon and Yangsan) to assess PCE reductive dechlorination activity. We also adapted molecular techniques to screen what types of known reductive dechlorinators are present at the PCE-contaminated aquifers. In the Changwon A and Changwon B active microcosms where potential electron donors such as sodium propionate, sodium lactate, sodium butyrate, and sodium fumarate, were added, ethylene, an end-product of complete reductive dechlorination of PCE, was detected after a period of 90 days of incubation. In the Bucheon and Yangsan active microcosms, cis-1,2-dichloroethylene (c-DCE) was accumulated without the production of vinyl chloride (VC) and ethylene. Molecular techniques were used to evaluate the microbial community structures in the Changwon B and Yangsan aquifer. We found two sequence types that were closely related to a known PCE to ethylene dechlorinator, named uncultured bacterium clone DCE47, in the Changwon B site clone library. However, in the Yangsan site clone library, no sequence type was closely related to known PCE dechlorinators reported. It is plausible that microorganisms being capable of completely dechlorinating PCE to ethylene may be present in the Changwon B site aquifer. In this study we find that complete PCE reductive dechlorinators are present at some PCE-contaminated sites in Korea. In an engineering point of view this information makes it feasible to apply a biological reductive dechlorination process for remediating PCE- and/or TCE-contaminated aquifers in Korea.
Purpose: Small size of recombinant scFv antibody has many advantages such as rapid blood clearances and improved targeting antibodies to tumor region. On the other hand owing to small size, number of amino group is insufficient in conjugation with chelator and fluorescence labeling. This study is to introduce poly lysine tag to the C-terminal end of scFv lym-1 sequence for fluorescence chelator conjugation. Materials and Methods: Poly lysine scFv lym-1 gene, cloned into pET-22b (+) vector, was expressed in E. coli BL21 (DE3) strain. Antibody purification was performed with Ni-NTA column and then size exclusion column chromatography. Expression and purification levels of poly lysine tagged scFv lym-1 antibody were confirmed by western blot analysis. I-124, I-125, I-131 and Tc-99m were used for radiolabeling of purified poly lysine scFv lym-1. Flow cytometry analysis of FIT( conjugated poly lysine scFv lym-1 was performed for confirmation of immunoreactivity of human Burkitt's lymphoma cells. Results: Poly lysine scFv lym-1 antibody was purified through two steps and identified as molecular weight of 48 KDa. Radiolabeling yields of I-124, I-125, I-131 and Tc-99m into poly lysine scFv lym-1 were >99%, >99%, >95% and >99%, respectively. Flow cytometry analysis of poly lysine scFv and scFv lym-1 was showed similar immunoreactivity to human Burkitt's lymphoma cells. Conclusion: Poly lysine tag was useful for the sufficient number of amino groups to scFv lym-1 antibody for chelator conjugation with minimizing loss of immunoreactivity.
This experiment was conducted to determine the Effect of soybean oil, dried palm oil powder and monoglyceride supplementation on growth performance and serum lipid changes in weaned pigs. One hundred twenty five cross-bred pigs((Y×L)×D, 6.00±0.79kg average initial BW) were used in a 21 d growth trial. Dietary treatments included SOY(containing 5% soybean oil), PALM(containing 5.5% dried palm oil powder) and SOPM0, 12.5 and 25(containing 2.5% soybean oil and 2.7% dried palm oil powder containing 0%, 12.5% and 25.0% monoglyceride, respectively). For the whole period, ADFI was increased in dried palm oil powder containing monoglyceride and PALM treatments compared with SOY and SOPM0 treatment(P<0.05). ADG and G/F were not affected by treatments. Digestibility of fat was higher(P<0.05) for pigs fed SOY diet than fed other diets. SOPM diets containing monoglyceride showed increased fat digestibility compared with PALM diet(P<0.05). SOPM12.5 and SOPM25 treatments were no difference with SOY treatment. However, there were no significant differences in digestibilities of DM, N and DE(P>0.05). Backfat thicknesses were not significantly different among treatments. Pigs fed SOY diet was decreased in serum total cholesterol, HDL-cholesterol, total lipid and was increased in free fatty acid than those fed other diet. Also, triglyceride concentration in serum was increased in PALM treatment compared with SOPM treatment(P=0.06). In conclusion, feeding soybean oil in weaned pigs showed higher fat digestibility and lower cholesterol and triglyceride concentration in serum than feeding only dried palm oil powder. Also, pigs fed monoglyceride were improved fat digestibility compared with those fed dried palm oil powder only.
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