• Korean Journal of Organic Agriculture
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• v.27 no.2
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• pp.147-160
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• 2019

This study was conducted to evaluate roughage to concentrate ratio on the changes of productivity and metabolic profiling in milk. Six lactating Holstein cows were divided into two groups, T1 group was fed low-concentrate diet (Italian ryegrass to concentrate ratio = 8:2) and T2 group was fed high-concentrate diet (Italian ryegrass to concentrate ratio = 2:8). Milk samples were collected and its components and metabolites were analyzed by ¹H-NMR (Nuclear magnetic resonance). The result of milk components such as milk fat, milk protein, solids-not-fat, lactose and somatic cell count were not significantly different between two groups. In carbohydrate metabolites, trehalose and xylose were significantly higher (P<0.05) in T1 group, however lactose was not significantly different between two groups. In amino acid metabolites, glycine was the highest concentration however, there was no significant difference observed between two groups. Urea and methionine were significantly higher (P<0.05) in the T2 group. In lipid metabolites, carnitine, choline and O-acetylcarnitine there were no significant difference observed between the two groups. In benzoic acid metabolites, tartrate was significantly higher (P<0.05) in T2 group. In organic acid metabolites, acetate was significantly higher (P<0.05) in T1 group and fumarate was significantly higher (P<0.05) in T2 group. In the other metabolites, 3-methylxanthine was only significantly higher (P<0.05) in T2 group and riboflavin was only significantly higher (P<0.05) in T1 group. As a result, milk components were not significantly different between two groups. However, metabolites concentration in the milk was significantly different depends on roughage to concentrate ratio.

• Journal of Animal Science and Technology
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• v.53 no.5
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• pp.469-474
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• 2011

This study was conducted to investigate the effects of application of composted cattle manure (CCM) on the productivity of the whole crop rice (WCR) and Italian ryegrass (IRG) on both Sancheong and Asan area from 2005 to 2008. Application levels of CCM such as chemical fertilizer (CF), CCM 150% and CCM 200% were tested in paddy field. Amount of CCM was adjusted equal to total nitrogen value relative to its value of CF. Mean dry matter yield (DMY) of WCR and IRG increased in order; CCM 200% > CF > CCM 150% in Asan and there was no significant difference between CCM 200% and CF. In Sancheong, DMYs of WCR and IRG significantly decreased (p<0.05) in order CF > CCM 200% > CCM 150%. The plant length and number of tiller of WCR were not different among treatments, however the percentage of ripeness of WCR was higher (p<0.05) in CF compared to CCM 150% and CCM 200%. Also, there were no differences in NDF and ADF contents, however CP content was higher (p<0.05) in CF treatment compared to CCM 150% and CCM 200%. Based on the results of this experiment, IRG-WCR double cropping system with application of CCM may be effective in producing the forage resources of 18~20 tons every year with eco-friendly way.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.2
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• pp.137-144
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• 2002

To investigate the short-term effects of N-supply form ( $NO_3\;^-$ or $NH_4\;^+$ ) and their level (0.2, 1.0 and 6.0 mM) on N assimilation and C metabolism were examined in perennial ryegrass (Lolium perenne L.). The increase in shoot fresh for $NH_4\;^+$-fed plants much less than $NO_3\;^-$ fed ones. Nitrate concentration in $NO_3\;^-$ -fed plants tended to increase with increasing the supply level, while that of $NH_4\;^+$-fed plants was nearly stable. Nitrate reductase activity (NRA) responded much quickly, showing a proportional increase within 24 h of feeding. NRA in $NO_3\;^-$ -fed plants at 72 h increased by 13.7, 40.3 and 84.0% in 0.2, 1.0 and 6.0 mM $NO_3\;^-$ -fed, but it was not changed in$NH_4\;^+$-fed plants regardless of the supply level. After 72 h of treatment the sugar accumulation in the plants supplied with 0.2 and 1.0 mM -$NH_4\;^+$fed was remarked. After 72 h of feeding, fructan hydrolysis was observed in all levels fur $NH_4\;^+$-fed plants, but only in 6.0 mM for $NO_3\;^-$ -fed plants.

• Journal of Animal Science and Technology
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• v.53 no.4
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• pp.303-310
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• 2011

Phylogenetic relationships of Jeju dogs to other domestic and foreign dog breeds were assessed using mtDNA D-loop sequences. Neighbor-joining trees were constructed using complete sequences (970 bp excluding the tandem repeat region) determined for five Cheju, four Jindo, four Sapsaree, five Pungsan, two of each East and West Laika dogs (Canis familiaris), two gray wolves (Canis lupus) and two coyotes (Canis latrans) and also published complete sequences for dogs. Coyote sequences were used as outgroups. In addition, a total of 214 haplotypes of 598bp D-loop sequences from 30 dog breeds were collected from GenBank and used to investigate genetic structure of population. In the analyses of full D-loop sequence variation and the phylogenetic trees constructed by neighbor-joining method, neither haplotypes nor clades specific for any domestic dog breeds were observed. The inter-species sequence variation (4.51%) between domestic dogs and wolves was much higher than the intra-species sequence variation within domestic dogs (1.63%) and wolves (3.64%). The divergence of the dog and wolf occurred approximately 1~2 million years ago based on these values. The taxa of Jeju dog breed in the phylogenetic tree are clustered separately and intermingled with other taxa of breeds, suggesting that active crossbreeding of Jeju dogs with other domestic breeds.

• Korean Journal of Environmental Agriculture
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• v.28 no.2
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• pp.131-138
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• 2009

Heavy metal residues in soil, rice straw, unhulled rice, rice hull, polished rice, and rice barn on the rice paddy in the "Top rice production complex which is non-contaminated area were evaluated. It was observed that the average concentrations of As, Cd, Cu, Pb, and Hg in the paddy soils were 1.235, 0.094, 4.412, 4.728 and 0.0279 mg/kg, respectively. There were no cultivation areas exceeded of the threshold for soil contamination designated by "The Soil Environment Conservation Law" in Korea. For the polished rice, there were no samples exceeded of a permissible level of heavy metal residues such as 0.051 mg/kg of As, 0.040 mg/kg of Cd, 0.345 mg/kg of Cu, 0.065 mg/kg of Pb and 0.0015 mg/kg of Hg. For the uptake and translocation of heavy metals to rice plant, a main part of heavy metal accumulation was rice straw, and then rice bran. Furthermore, it shown that accumulation of heavy metals in unhulled rice, rice hulls, brown rice, and polished rice was approximately similar as low. The slopes of translocation of heavy metals from soil to polished rice were following order as Cd, 0.4321 > Cu, 0.054 ${\fallingdotseq}$ Hg, 0.052 > As, 0.021 > Pb, 0.008. It was observed that potential ability of Cd uptake in rice plant and then its translocation into polished rice was very high. Concentrations of copper and mercury absorbed in the rice plant were moderate for translocating into the polished rice, while the arsenic and lead in the plant were scarcely translocated into the polished rice. The distribution of heavy metals absorbed and translocated into aboveground parts of rice plant was appeared that there were remained at 63.3-93.4% in rice straw, 6.6-36.9% in unhulled rice, 0.6-5.7% in rice hulls, 3.2-31.3% in brown rice, 0.8-4.6% in rice bran and 1.1-26.7% in polished rice. The accumulation ratio of Cd in the aboveground parts of rice plant was remained at 26.7-31.3% in brown and polished rice.

• The Journal of the Korea Contents Association
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• v.8 no.8
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• pp.285-292
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• 2008

This study was initiated to evaluate the Phosphorus(P) leaching potential in the putting green soils and P uptake by the turfgrass in the golf course using the P fertilizers. The turfgrass, Floradwarf bermudagrass(Cynodon dactylon L. PERS,) was planted and grown in the mixture of sand and peat moss in this lysimeter study. Five representative P fertilizers, such as, ammonium polyphosphate (APP), monopotassium phosphate (MKP), MAP(monoammonium phosphate), 0-20-20(liquid), and concentrated superphosphate(CSP, solid) were used in this study. Based on the total P quantity of leachate collected during the whole 12 weeks, MKP and APP are the first group of P fertilizers contributing to the leaching of P, then MAP and 0-20-20 are the second group of P fertilizers causing the P leaching. Finally, CSP is the third group of P fertilizer resulting in the P leaching. However, most of P applied and collected in the lysimeter were leached during the first period of two and four weeks, compared to that of P leached during the second period of six, eight, ten, and twelve weeks. Applications of MAP, APP and CSP, MKP and 0-20-20 in order produced the largest amount of total dry matter. However, APP, MKP and MAP, CSP and 0-20-20 in order showed the largest amount of P uptake. Therefore, based on the data of P leaching, dry matter production, and plant P uptake, it appears that CSP, 0-20-20, and MAP are the environmentally sound fertilizers recommended in the turfgrass putting green soil of golf course.

• Journal of Life Science
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• v.20 no.3
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• pp.411-415
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• 2010

Anisakis simplex is one of the parasitic nematodes, and has a complex life cycle in crustaceans, fish, squid or whale. When people eat under-processed or raw fish, it causes anisakidosis and also plays a critical role in inducing serious allergic reactions in humans. However, no web-based database on A. simplex at the level of DNA or protein has been so far reported. In this context, we constructed a web-based database for Anisakis research. To build up the web-based database for Anisakis research, we proceeded with the following measures: First, sequences of order Ascaridida were downloaded and translated into the multifasta format which was stored as database for stand-alone BLAST. Second, all of the nucleotide and EST sequences were clustered and assembled. And EST sequences were translated into amino acid sequences for Nuclear Localization Signal prediction. In addition, we added the vector, E. coli, and repeat sequences into the database to confirm a potential contamination. The web-based database gave us several advantages. Only data that agrees with the nucleotide sequences directly related with the order Ascaridida can be found and retrieved when searching BLAST. It is also very convenient to confirm contamination when making the cDNA or genomic library from Anisakis. Furthermore, BLAST results on the Anisakis sequence information can be quickly accessed. Taken together, the Web-based database on A. simplex will be valuable in developing species specific PCR markers and in studying SNP in A. simplex-related researches in the future.

• Proceedings of the KSCN Conference
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• 2003.11a
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• pp.1058-1059
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• 2003

학동기(만 6-12세)에 좋은 영양 공급은 일생의 성장 발육의 기초를 조성하여 지적, 사회적, 정서적 능력을 향상시킬 수 있다. 이에 초등학생의 영양문제와 식습관을 해결하는 방안의 하나로 최근 대두되고 있는 아침결식률을 낮추기 위한 ‘아침 안 거르기’ 사업을 학교와 연계 개발하여 프로그램을 진행하고 그 효과를 평가하였다. 이 연구는 서울시 강북구 관내에 위치한 4개 초등학교 5학년 학생 1026명(실험군 451명, 대조군 675명)을 대상으로 4∼9월까지 6개월간 5단계로 실시되었다. 프로그램의 효과평가를 위해 사전(실험군-학생 428명, 부모 416명, 대조군-학생 656명, 부모 589명), 사후(실험군-학생 427명, 부모 368명, 대조군-학생 608명, 부모 558명) 설문조사를 2회 실시하여 행동과 인식률 변화를 측정하였다. 프로그램은 매월 1회 담임교사가 진행하였고, 교육자료 및 홍보물은 아침먹기 캐릭터 가방걸이와 학부모의 참여를 높이고자 가정통신문과 교육내용을 함께 삽입하여 제작한 알림장, 아침먹기와 일찍자기를 실천할 실천스티커를 배포하였고 프로그램 종료 후 아침 먹기 실천달력을 제공하였다. 또 아동들 자신의 식생활을 바르게 꾸려 나갈 수 있도록 가공식품의 ‘영양표시’에 관한 교육을 함께 교육하였고, 설문조사를 통해 영양표시제에 관한 인식변화와 행동변화율을 측정하였다. 학생들의 아침식사 실천율은 매일 먹는 그룹이 실험군은 62.9%에서 69.6%로 7% 상승하였고, 전혀 먹지 않는 그룹은 11.2%에서 6.4%로 4.8% 감소하였다(p<0.001) 대조군의 경우 매일 먹는 그룹은 70.4%에서 70.2%로, 전혀 먹지 않는 그룹은 6.9%에서 6.5%로 나타났다(p<0.001). 아침식사가 건강에 미치는 영향에 대한 인식률 변화는 큰 영향이 있다고 답한 그룹이 실험군은 60.1%에서 64.8% (p<0.001)로, 대조군은 64.8%에서 57.2% (p<0.001)로 각각 변화하였다. 일찍 자기가 건강에 미치는 영향에 대한 인식률은 큰 영향이 있다고 답한 그룹이 실험군은 52.5%에서 70.7%(p<0.001), 대조군은 55.3%에서 60.2%로(p<0.001), 일찍 자기 실천에 대한 의지 변화는 11시 이전에 잘 계획이라는 그룹이 실험군은 31.9%에서 43.9%(p<0.001), 대조군은 35.5%에서 36.8%로 나타났다(p<0.001). 영양 표시에 관한 문항에서는 식품구매시 영양표시를 보고 구매한 경험이 없다는 군이 실험군에서는 58.2%에서 40.4%로 감소하였고(p<0.001), 대조군은 64%에서 53.6% (P<0.001)로 감소하였다. 영양소에 관한 지식 평가에서 짜지 않은 간식을 선택할 때 유의할 성분을 나트륨이라고 답한 그룹은 실험군은 31.7%에서 56.3%로, 대조군은 15.9%에서 23.0%로 나타나 그룹간 큰 차이를 보였다. 아침식사로 좋아하는 음식은 김치, 김치찌개, 밥, 피자, 고기, 빵, 된장찌개, 김, 햄, 계란후라이 순이었으며 싫어하는 아침음식은 피망, 고추, 양파, 김치, 시금치, 마늘, 콩, 파, 피자, 나물순으로 나타났다. 아침식사로 혼자서도 준비할 수 있는 음식은 밥, 라면, 계란후라이, 김치, 반찬, 볶음밥, 계란, 국, 햄, 씨리얼 순으로 나타났다. 부모의 인식률 변화는 가정내에 컴퓨터 설치장소로 적합하다고 생각되는 장소로 실험군의 경우 학생 방은 28.0%에서 21.6%로 6.4%감소하였고, 마루나 거실은 61.3%에서 66.0%로 5%증가하였다(p<0.001). 반면 대조군의 경우 학생 방은 28.8%에서 24.9%로 3.9% 감소, 마루나 거실은 63.4%에서 65.9%로 2.5%증가하여 실험군과 큰 차이를 보였다(p<0.001) 자녀의 늦은 취침시간과 아침식사에 관한 인식률 변화는 아침식사에 영향이 있다는 군은 실험군의 경우 78.1%에서 82.7%로 4.2% 증가하였고(p<0.05), 대조군의 경우 82.2%에서 80.5%로 감소하여 나타났다.(P<0.001) 연구결과 실험군의 경우 일주일 동안 아침을 전혀 먹지 않는 학생이 감소하고 매일 아침을 먹는 학생이 유의적으로 증가하여 아동의 아침식사 행동변화와 인식률 변화에 도움이 된 것으로 나타났다.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.47 no.2
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• pp.21-31
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• 2010

This paper presents a design of PLL and SSCG for reducing the EMI effect at the electronic machinery and tools for DisplayPort application. This system is composed of the essential element of PLL and Charge-Pump2 and Reference Clock Divider to implement the SSCG operation. In this paper, 270MHz/162MHz dual-mode PLL that can provide 10-phase and 1.35GHz/810MHz PLL that can reduce the jitter are designed for 2.7Gbps/162Gbps DisplayPort application. The jitter can be reduced drastically by combining 270MHz/162MHz PLL with 2-stage 5 to 1 serializer and 1.35GHz PLL with 2 to 1 serializer. This paper propose the frequency divider topology which can share the divider between modes and guarantee the 50% duty ratio. And, the output current mismatch can be reduced by using the proposed charge-pump topology. It is implemented using 0.13 um CMOS process and die areas of 270MHz/162MHz PLL and 1.35GHz/810MHz PLL are $650um\;{\times}\;500um$ and $600um\;{\times}\;500um$, respectively. The VCO tuning range of 270 MHz/162 MHz PLL is 330 MHz and the phase noise is -114 dBc/Hz at 1 MHz offset. The measured SSCG down spread amplitude is 0.5% and modulation frequency is 31kHz. The total power consumption is 48mW.

• Korean Journal of Soil Science and Fertilizer
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• v.35 no.4
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• pp.232-242
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• 2002

To investigate the effects of arbuscular mycorrhizal (AM) fungus (Glomus intraradices) colonization on drought-stress tolerance, leaf water potential, chlorophyll concentration, P content and carbohydrate composition were examined in perennial ryegrass (Lolium perenne L.) plants exposed to drought-stressed or well-watered conditions. Drought stress significantly decreased leaf water potential, P content and leaf growth. These drought-induced damages were moderated by mycorrhizal colonization. Drought stress decreased the concentration of soluble sugars in shoots. AM plants had a higher foliar soluble sugar than non-AM plants under drought stress condition. Drought stress depressed the accumulation of starch and fructan in shoots, but stimulated in roots. Under drought-stressed condition, starch concentration in roots was higher in non-AM plants than in AM plants. Fructan was the largest pool of carbohydrates, showing the highest initial concentration and the highest net increase for 28 days of treatment. Drought stress slightly decreased fructan concentration in shoots, but remarkably increased in roots. Under drought-stressed condition, fructan concentrations in non-AM and AM shoots at day 28 were 18.7% and 13.3% lower than the corresponding values measured at well-watered plants. However, in the roots, fructan accumulation caused by drought was lessen 13.6% by mycorrhizal colonization. The results obtained suggest that mycorrhizal colonization improves drought tolerance of the host plants by maintaining higher leaf water status and P status, and by retaining more foliar soluble sugars.