Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Nitrogen Assimilation and Carbohydrate Concentration as Affected by the N Supply Form and Their Level in Shoot of Perennial ryegrass (Lolium perenne L.)

페레니얼 라이그라스에서 질소공급형태 및 수준에 따른 질소동화와 탄수화물 대사산물의 변화

  • Lee, Bok-Rye (Department of Animal Science & Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University) ;
  • Jung, Woo-Jin (Department of Animal Science & Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University) ;
  • Kim, Dae-Hyun (Department of Animal Science & Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University) ;
  • Kim, Kil-Yong (Department of Biological & Environment Chemistry, College of Agriculture and Life Science, Chonnam National University) ;
  • Kim, Tae-Hwan (Department of Animal Science & Institute of Agricultural Science and Technology, College of Agriculture and Life Science, Chonnam National University)
  • Published : 2002.06.01
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Abstract

To investigate the short-term effects of N-supply form ( $NO_3\;^-$ or $NH_4\;^+$ ) and their level (0.2, 1.0 and 6.0 mM) on N assimilation and C metabolism were examined in perennial ryegrass (Lolium perenne L.). The increase in shoot fresh for $NH_4\;^+$-fed plants much less than $NO_3\;^-$ fed ones. Nitrate concentration in $NO_3\;^-$ -fed plants tended to increase with increasing the supply level, while that of $NH_4\;^+$-fed plants was nearly stable. Nitrate reductase activity (NRA) responded much quickly, showing a proportional increase within 24 h of feeding. NRA in $NO_3\;^-$ -fed plants at 72 h increased by 13.7, 40.3 and 84.0% in 0.2, 1.0 and 6.0 mM $NO_3\;^-$ -fed, but it was not changed in$NH_4\;^+$-fed plants regardless of the supply level. After 72 h of treatment the sugar accumulation in the plants supplied with 0.2 and 1.0 mM -$NH_4\;^+$fed was remarked. After 72 h of feeding, fructan hydrolysis was observed in all levels fur $NH_4\;^+$-fed plants, but only in 6.0 mM for $NO_3\;^-$ -fed plants.

페레니얼 라이그라스에서 질소의 공급형태 ( $NO_3\;^-$ or $NH_4\;^+$) 및 수준 (0.2, 1.0 and 6.0mM)에 따른 질소동화와 탄수화물 대사산물에 미치는 영향을 알아보기 위해 nitrate, nitrate reductase, sugar 농도와 Fructan 농도를 조사하였다. $NH_4\;^+$공급구에서 잎의 생체량은 약간 증가하다가 같은 수준으로 유지되는 반면 $NO_3\;^-$ 공급구에서는 농도가 증가함에 따라 처음수준에 비해 약 25%에서 30% 증가하였다. $NO_3\;^-$ 공급구에서 Nitrate 농도는 Nitrate 농도가 더 높을 때 현저히 증가하는 반면에 $NH_4\;^+$ 공급구에서는 유의적인 변화가 없었다. Nitrate reductase activity(NRA)는 초기수준에 비해 0.2, 1.0 and 6.0mM $NO_3\;^-$ 공급구에서 13.7, 40.3 and 84.0% 각각 증가하였다.$NH_4\;^+$공급구는 실험기간 동안 유의적인 차이가 없었다. Sugar의 축적은 $NH_4\;^+$공급구에서 뚜렷이 나타났으며 특히 공급수준이 가장 낮은 0.2mM에서 107.2 mg $NO_3\;^-$ DW로 다른 공급수준에 비해 가장 높게 나타났다. $NO_3\;^-$ 공급구에서 sugar의 농도는 초기수준에 비해 같은 수준으로 유지되거나 약간 감소하는 경향을 보였다. 처리 후 72시간에서 fructan 농도를 비교할 때 $NH_4\;^+$공급구의 경우 3 공급수준에서 공히 유의적으로 감소하였으나, $NO_3\;^-$ 공급구에서는 약간 증가하거나 초기 수준과 비슷하게 유지되었다.

Keywords

References

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