• Korean Journal of Plant Taxonomy
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• v.40 no.2
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• pp.118-129
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• 2010

A taxonomic study of Viola albida complex, containing the representative individuals of three taxa, V. albida var. albida, V. albida var. chaerophylloides, and V. albida var. takahashii, was done based on RAPD data. The amplified loci were 476 in total; obtained with 68 universal primers on seven OTUs. Nei's genetic dissimilarity appeared relatively low within individuals of V. albida var. albida and V. albida var. chaerophylloides (0.118-0.171 and 0.051 respectively), however, it was higher in individuals of V. albida var. takahashii (0.348). On the other hand, there is no specific trend in terms of genetic dissimilartiy among taxa, such as between individuals of V. albida var. albida and V. albida var. takahashii, between those of V. albida var. albida and V. albida var. chaerophylloides, and between those of V. albida var. albida and V. albida var. takahashii. The similarity of OTUs studied is high in clustering analysis, so that this result is compatible with the establishment of this complex. All OTUs are clustered within two groups. The individuals of V. albida var. takahashii, however, are clustered both to the group of V. albida var. albida and to the group of V. albida var. chaerophylloides, meaning that the genetic difference is high which would be commensurate with their morphological variations.

• Korean Journal of Plant Taxonomy
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• v.36 no.3
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• pp.163-187
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• 2006

Taxonomic studies were conducted to evaluate the interspecific relationships in Viola albida var. albida and its related taxa using seven populations for morphology, palynology and anatomy. Molecular phylogenetic studies were also examined in 28 populations including 19 Korean, four Chinese, two Japanese, one American population and two outgroups using nrITS, and 27 populations except V pinnata for trnL-F region of chloroplast DNA. Morphological differences was observed among seven populations of three species in leaf shape, but characters such as serrate number of leaf margins, petal size, pistil shape were showed overlap between populations. Pollen shape of seven populations was monad and grain shape on the polar axis was semi-angular. Morphology of aperture was tri-colporate, and the surface sculpturing was scabrate in rugulate. The grain shape of equatorial view of five populations was prolate whereas V albida var. taknhashii type 1 and V albida var. chaerophylloides type 3 were subprolate. The anatomical characters of rnidvein of leaf, petiole, peduncle, root were also described for the species. The stomatal apparatus of the leaves was observed only in abaxial surface, and the number of stoma per unit ($mm^2$) were abundant in incised than lobate or cleft margin of the leaves. The nrITS analysis shows that V. pinnata and V. dissecta was monophyletic and occupied a basal position in the V. albida var. albida and its related taxa. The other clade including infraspecific populations of V. albida, and V. eizanensis was paraphyletic. The trnL-F noncoding region analysis was similar to the ITS tree. According to the above observations in morphology, palynology, anatomy, and molecular phylogenetic analysis, the significant differences were not found except for leaf shape in Viola albida var. albida and its related taxa, therefore V. albida var. takanhashii and V. albida var. chaerophylloides were considered to be an infraspecific taxa of V. albida var. albida rather than an independent species, subvariety or variety of V. pinnata and V. dissecta.

• Korean Journal of Plant Resources
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• v.19 no.5
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• pp.628-633
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• 2006

ITS DNA sequences from five individuals, representative of five groups designated according to the degree of leaf teeth and lobes from simple to palmate compound leaf in the Viola albida complex, established and further analysed in order to solve the taxonomic difficulty. A total 702 bp was sequenced at the 5.8S ribosomal DNA and internal transcribed spacer 1 and 2. The 5.8S coding region is 163 bp, and has no sequence variations. The ITS1 and ITS2 noncoding regions have a little bit sequence variations, and those were further analysed by the methods of the analysis of variance (ANOVA), the analysis of sequence divergence and the phylogenetic analysis. The result of ANOVA showed no significant differences among individuals investigated. The analysis of sequence divergence with Kimura 2-parameter distance revealed that in-groups showed much less than 0.05 in absolute value among individuals, while two out groups more than 0.05, V. grypoceras and V. orientalis. This result appeared that the sequence divergence among in-groups was not yet occurred in the species level but situated at somewhere below the species level. In the phylogenetic analysis, two outgroups formed the basal clades in order. Five individuals in-groups formed a clade. The clade was, however, not very robust as around 50% in bootstrap value, suggesting that this result was not meaningful in the phylogenetic point of views.

• The Korean Journal of Ecology
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• v.23 no.3
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• pp.255-266
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• 2000

생물권보전지역으로 지정된 점봉산의 온대낙엽수림(참나무 성숙림)에서 임상초본식물의 공간분포를 정량화하고, 이들의 공간분포에 미치는 미소환경요인의 영향을 파악하였다. 대부분의 임상초본식물들은 영구 방형구내 미소지소간에 큰 변이를 나타내었다. 초봄에 출현하는 임상초본식물중 공간의존도가 높은 식물은 현호색, 애기나리 및 노랑제비꽃이었고, 가을에 출현하는 임상초본식물중 공간의존도가 높은 식물은 벌깨덩굴, 쌀새 및 도라지모시대이었다. 초봄에 출현하는 홀아비바람꽃과 얼레지 그리고 가을에 출현하는 도라지모시대와 쌀새의 공간의존거리가 상대적으로 짧은 편이었다. 초봄에 출현하는 노랑제비꽃, 애기나리 및 얼레지는 관목이 적고, 낙엽층이 얇으며,토심이 깊고, 토양수분함량, 유기물함량 및 염기성양이온함량이 상대적으로 적은 생육지를 선호하였고, 애기앉은부채 와 현호색은 관목이 밀집하고, 낙엽층이 두꺼우며, 토심이 얇고, 유기물함량, 토양수분함량 및 영양염류함량이 높은 생육지를 선호하였다. 오리방풀은 빛조건이 좋고, 낙엽층이 두꺼우며, 토양수분함량과 영양염류가 풍부한 생육지를 선호하였고, 단풍취, 대사초, 개별꽃, 참취, 쌀새 및 도라지모시대는 토심이 깊고, 낙엽층이 얇으며, 토양수분함량과 영양염류함량이 상대적으로 적은 생육지를 선호하였다. 본 참나무 성숙림에서 토양수분함량, 유기물함량 및 영양염류함량은 바람, 미소지형 및 관목의 밀집정도에 의해 공간적으로 재배치되는 낙엽층 두께에 의해 영향을 받고 있기 때문에 초봄과 가을에 관찰되는 임상초본식물의 공간분포는 낙엽층의 불균등한 분포에 의해 가장 큰 영향을 받고 있는 것으로 추정되었다.

• Journal of Korea Foresty Energy
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• v.20 no.2
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• pp.81-116
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• 2001

The flora of vascular plants investigated in civilian control line area was 730 taxa consisting of 104 families, 331 genera, 631 species, 2 subspecies, 90 varieties, 7 formae. The forest was comparatively well conserved by Pteridophyta calculation(Pte-Q), 1.27. Among the investigated vascular plants, Korean endemic plants(Carex okamotoi, Salix koriyanagi, Clematis chisanensis, Aconitum pseudo-laeve var. erectum, Viola diamantiaca etc.) were 31 taxa; rare and endangered plants(Disporum ovale, Lilium distichum, Trillium kamschaticum, Gastrodia elata etc.) were 35 taxa; naturalized plants(Dactylis glomerata, Poa pratensis, Rumex acetosella, Gypsophila oldamiana, Lepidium apetalum etc.) were 26 taxa. The forest vegetation was classified into 1 order, 1 alliances, 6 communities; Rhododendro-Quercetalia mongolicae Kim 1990(신갈나무-철쭉군목), Lindero-Quercion mongolicae Kim 1990(신갈나무-생강나무군단), A. Carex humilis v. nana- Phinus densiflora Community 소나무-산거울군락, B. Acer pseudo-sieboldianum-Quercus mongolica Community 당단풍-신갈나무군락, C. Styrax obassia-Quercus mongolica Community 쪽동백-신갈나무군락, D. Acer palmatum-Quercus mongolica Community 단풍나무-신갈나무군락, E. Rheodendron mucronulatum-Quercus mongolica Community 진달래-신갈나무군락, F. Betula schmiditii-Quercus mongolica Community 박달나무-신갈나무군락

• Korean Journal of Plant Taxonomy
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• v.41 no.1
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• pp.66-80
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• 2011

This study investigated the environmental factors and conducted a RAPD analysis for a better understanding of the environmental characteristics and regional genetic variation in samples from 18 different areas of Viola diamantiaca. The habitats are mostly located on the slopes of mountains facing north at an altitude ranging from 614 m to 1,462 m above sea level with angles of inclination ranging from 3 degrees to 30 degrees. A total of 268 vascular plant taxa are identified in 35 quadrates of 18 habitats. The importance value of V. diamantiaca is 11.58%, and four highly ranked species, Sasa borealis (5.61%), Meehania urticifolia (5.21%), Ainsliaea acerifolia (3.62%), Pseudostellaria palibiniana (3.60%) are considered to have an affinity with V. diamantiaca in their habitats. The degree of their average species diversity is 1.36, while this metric for their evenness and dominance are 0.89 and 0.07, respectively. The average field capacity of the soil is 25.99%, with organic matter at 17.47%, and the pH is 5.19. The soil texture was confirmed as sandy loam of eleven and loam of seven. The result of the RAPD analysis, among 78 bands amplified with a primer, 64 (84.6%) showed polymorphism. Eighteen populations could be classified into five groups with similarity coefficient values ranging from 0.53 to 0.86. The Mt. Jiri population, which is geographically segregated, shows basal branching within the 18 populations. Five populations, including two in the southern district in Gangwon-do and three in Chungcheongbuk-do, form a distinct clade. Four populations in the central district of Gangwon-do and Mt. Bohyeon in the Gyeongsangbuk-do clade form a sister to the clade containing two populations in Gyeonggi-do and five populations of the northern district in Gangwon-do. The Mt. Gariwang population is placed between the southern district and the central district in the Gangwon-do clades.

• Korean Journal of Plant Resources
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• v.24 no.5
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• pp.549-556
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• 2011

We investigated the plant phenology on the threatened species for climate change in the summit area of Mt. Deogyusan which is a representative sub-alpine zone in Korea. We had performed the monitoring survey of plant phenology on 38 species including 20 trees and 18 herbs from May 2009 to November 2010. The investigated phenological charateristics were five dates for leafing, flowering, floral abscission, autumn leaf colors and leaf abscission on each plant species in sub-alpine region. The climate data were measured from November 2009 to December 2010. The range of temperature was from 30.4 to -$20.3^{\circ}C$ at Hyangjeokbong to Jungbong region, and the relative humidity was 100% to 3.4%. The leafing dates in 2010 were similar to 2009 or were 6-20 days delayed in most of the investigated species except Veratrum oxysepalum and Sanguisorba hakusanensis which showed 8 days earlier leafing dates in 2010. The biggest difference among phenological characters was found in flowering dates. The flowering dates of early Spring blooming species such as Heloniopsis koreana, Rhododendron yedoense for. poukhanense and Viola orientalis showed 13-20 days earlier in 2010, and the several summer flowering species as Viburnum opulus var. calvescens, Smilacina japonica and Bupleurum longeradiatum showed 6-10 days delay in 2010. The dates for floral abscission and autumn leaf colors in 2010 were delayed about 10-18 days, and leaf abscission dates were similar to 2009. The effects of climate change on the phenology for the threatened species in sub-alpine zones of Korea are occuring especially on flowering, floral abscission and autumn leaf colors.

• Korean Journal of Plant Resources
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• v.12 no.2
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• pp.125-132
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• 1999

This study has investigated the growing features and vegetation of Acanthopanax chiisanensis and Acanthopanax koreanum in Mt. Jiri and Halla from Sep. 1997 to Aug. 1998. As to Acanthopanax chiisanensis, it occupied surrounding areas of Nogodan in Mt. Jiri of the height of 1,507m, ImgeolyoungJae of 1,300m and the Mt. Halla. of 1,100m of There were rather high constancy degree with Quercus mongolica, Schizandra chinensis, Hydrangen serrate for. acuminata, Acer pseudo-sieboldianum, Dryopteris crassirhizoma, Actaea asiatica, Thalictrum filamentosum, Abies koreana, Arundinella hirta from the sorts. The common species in Mt. Jiri and Halla are Acer pseudo-sieboldianum, Dryopteris crassirhizoma, Callicarpa japonica, Parthenocissus tricuspidata, Acer mono, Ligularia fischeri, Disporum smilacinum, Viola acuminata Aconitum kusnezofii. On the other hand, concerning of Acanthopanax koreanum community, it possessed the small areas of wedo weoltae village in Chejudo. In this area, a liana e.g. Dioscorea tenuipes, Hedera rhombea, Lygodium japonicum, Akebia quinata, Vitis thunbergii, Dunbaria villosa, Trachelospermum asiaticum var. intermedium, Vitis amurensis, Paederia scandens, lives with Pinux thunbergii, Celtis aurantiaca, Mallotus japonicus and so forth in tree layer and subtree layer. There is artificial change no move in the researching sites, Acanthopanax chiixanensis will be transformed into Abies koreana - Acanthopanax chiisanensis community. Soil characteristics showed the high value such as pH 6.56 and conductivity 0.258mmho/cm in Acanthopanax koreanum community of Mt. Jiri, they also indicated digit as follows: the content of organic matter and total nitrogen are from 25.16 through 25.35% and 7.58 through 9.30mg/g respectably. The soil characteristics at Acanthopanax chiisanensis community in Mt. Jiri and Halla, was high as like pH 5.76, conductivity 0.238mmho/cm, total nitrogen 9.30mg/g, total phosphorus 0.126mg/g in Mt. Halla.

• Journal of Korean Society of Forest Science
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• v.99 no.3
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• pp.267-276
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• 2010

This study was performed to obtain basic data about vegetation structure and site environment of rare and endangered plant, viola websteri in five natural habitats. The dominant species of Hongcheon habitat were Pyrus pyrifolia (tree layer), Morus bombycis (subtree layer), Pyrus pyrifolia (shrub layer) and Isodon inflexus (herb layer). Mt. Yeonin habitat were dominated by Quercus mongolica (tree layer), Morus bombycis (subtree layer), Philadelphus schrenckii (shrub layer) and Viola websteri (herb layer). Mt. Yumyung habitat were dominated by Cornus controversa (tree layer), Morus bombycis (subtree layer), Fraxinus rhynchophylla (shrub layer) and Adenocaulon himalaicum (herb layer). Mt. Daebu habitat were dominated by Acer mono (tree layer), Staphylea bumalda (subtree layer), Staphylea bumalda(shrub layer) and Dryopteris crassirhizoma (herb layer). And the dominant species of Mt. Worak habitat were Larix leptolepis (tree layer), Fraxinus rhynchophylla (subtree layer), Alangium platanifolium var. macrophylum (shrub layer) and Adenocaulon himalaicum (herb layer). It was found out that the average acidity of soil pH was 5.23; 0.30ds/m for electron conductivity; 12.6% for organic matter content; 0.68% for total nitrogen; 21.66 ppm for available phosphorous; and 23.45 cmol(+)/kg for CEC. Interchangeable cation was decreased in order of $Ca^{2+}$ > $Mg^{2+}$ > $K^{2+}$. Viola websteri had positive association with Polygonatum odoratum var. pluriflorum and Geranium thunbergii but negative association with Poa sphondylodes. Species diversity index(H'), maximum Species diversity index (H' max), evenness(J') and dominance (1-J'), of investigated sites ranged 0.6816 (Mt. Worak)~0.9656 (Mt. Daebu), 1.0000 (Mt. Yeonin)~1.3010 (Hongcheon, Gangwon), 0.5330 (Mt. Worak)~0.8947 (Mt. Daebu) and 0.1053 (Mt. Daebu)~0.4670 (Mt. Worak) in woody layer and 1.0414 (Mt. Yumyung)~1.3295 (Mt. Worak), 1.4314 (Mt. Yumyung)~1.6435 (Mt. Daebu), 0.6984 (Hongcheon, Gangwon)~0.8356 (Mt. Worak) and 0.1644 (Mt. Worak)~0.3016 (Hongcheon, Gangwon) in herb layer, respectively.

• Journal of Forest and Environmental Science
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• v.16 no.1
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• pp.1-16
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• 2000

This study was carried out to examine the process of plant succession through vegetation invasion and to select appropriate endemic plant species for rapid stabilization and good visual effect on cut-slopes of forest roads. Establishing total of sixty $1m{\times}1m$ sample plots. fifteen for each forest road constructed in the year of '93 (six-year-old), '95(four-year-old), '97(two-year-old), and '98(one-year-old), the ecological attributes of invading vegetation on cut-slopes were analyzed. The results are summarized as follows: 1. The rate of vegetation coverage was highly associated with soil hardness and aspect of cut-slope. Higher rate of vegetation coverage was caused by larger number of invading plant species. 2. The dominant woody species were Rubus crataegifolius, Rhus chinensis, Lespedeza bicolor, Salix hulteni, Alnus hirsuta, and Pinus densiflora. The visual attractive for the fruit of Rubus crataegifolius and the autumn coloration of Rhus chinensis was noteworthy. The dominant herbaceous species were Youngia sonchifolia, Spodiopogon sibiricus, and Lysimachia clethroides in all study forest roads. Spring flower of Potentilla freyniana and Viola rossii: summer flower of Lysimachia clethroides, Commelina communis, Glycine soja. Persicaria sieboldi, and Oenothera odorata: and autumn flower of Artemisia stolonifera and Impatiens textori were abundant and remarkable. 3. The diversity index of woody species tended to be increased as years passed after construction, and that of herbaceous species were decreased. 4. The dominance of Th of dormancy form was early high in the first year of construction, getting decreased thereafter. And that of MM + M + N was increased as years passed after construction. but that of Ch+H+G+Th+HH was decreased. 5. The degrees of succession were estimated by 359, 111, 97, and 87 for the construction year of '93, '95, '97, and '98. respectively, increased as years passed after construction.