• Title/Summary/Keyword: 단단법

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Antibacterial Effect of Various Fermentation Products and Identification of Differentially Expressed Genes of E.coli (다양한 발효액의 항균효과와 대장균의 유전적 변화에 미치는 영향)

  • Heo, Jihye
    • Korean Journal of Clinical Laboratory Science
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    • v.54 no.2
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    • pp.119-124
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    • 2022
  • Pseudomonas aeruginosa (P. aeruginosa), Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) are typical opportunistic pathogens. Moreover, these bacteria are known to possess multidrug-resistant (MDR) properties. This study investigates the antimicrobial activity of six fermented products, which have varying efficacies against P. aeruginosa, E. coli, and S. aureus. To identify novel candidate genes, differential expression analysis was performed using an annealing control primer. In the disk diffusion method, Fig vinegar (FV) and Diospyros kaki Thunb vinegar (DTV) showed the greatest increase in inhibition compared to other fermented products, whereas fermented Korean traditional nature herb (FKTNH) had no antibacterial effect. This study identified down-regulation of Escherichia coli O157:H7 ompW gene for outer membrane protein W, whereas gene for synthetic construct Lao1 gene for L-amino acid oxidase were up-regulated in E. coli treated with 5% FV. Consuming fermented vinegar helps prevent bacterial infections. Especially, FV and DTV are potentially useful alternative natural products for multidrug resistance. Furthermore, both are expected to be used as effective natural antimicrobial agents, such as disinfectants.

A Study on the Construction method of Stamped earthen wall (판축토성(版築土城) 축조기법(築造技法)의 이해(理解) - 풍납토성(風納土城) 축조기술(築造技術)을 중심(中心)으로 -)

  • Shin, Hee-kweon
    • Korean Journal of Heritage: History & Science
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    • v.47 no.1
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    • pp.102-115
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    • 2014
  • The stamped earth method is a typical ancient engineering technique which consists of in-filling wooden frame with layers of stamped earth or sand. This method has been universally used to construct earthen walls and buildings, etc. The purpose of this article is to understand the construction method and principles of the stamped earthen wall through analysis of various construction techniques of Pungnaptoseong Fortress(Earthen Fortification in Pungnap-dong). First of all, the ground was leveled and the foundations for the construction of the earthen wall were laid. The underground foundation of the earthen walls was usually constructed by digging into the ground and then in-filling this space with layers of mud clay. Occasionally wooden posts or paving stones which may have been used to reinforce the soft ground were driven in. The method of adding layers of stamped earth at an oblique angle to either side of a central wall is the most characteristic feature of Pungnaptoseong Fortress. Even though the traces of fixing posts, boards, and the hardening of earth - all signatures of the stamped earth technique - have not been identified, evidence of a wooden frame has been found. It has also been observed that this section was constructed by including layers of mud clay and organic remains such as leaves and twigs in order to strengthen the adhesiveness of the structures. The outer part of the central wall was constructed by the anti-slope stamped earth technique to protect central wall. In addition a final layer of paved stones was added to the upper part of the wall. These stone layers and the stone wall were constructed in order to prevent the loss of the earthen wall and to discharge and drain water. Meanwhile, the technique of cementing with fire was used to control damp and remove water in stamped earth. It can not be said at present that the stamped earth method has been confirmed as the typical construction method of Korean ancient earthen walls. If we make a comparative study of the evidence of the stamped earth technique at Pungnaptoseong Fortress with other archeological sites, progress will be made in the investigation of the construction method and principles of stamped earthen wall.

Ancient iron technologies as observed in the microstructures of iron sickles excavated from Icheon Seolbong fortress (이천 설봉산성 출토 철제 낫의 제작기술 연구)

  • Yu, Jae-Eun
    • Journal of Conservation Science
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    • v.20
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    • pp.67-80
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    • 2007
  • Icheon Seolbong fortress is located in Saum-dong, Icheon, Gyeonggi-do Province that its date is founded as 4th century of Bakjae dynasty after excavation by Dankook university museum. Excavated artifacts show various kinds such as earthenwares, ceramics, metal artifacts and so on. Especially many iron artifacts were founded in where Bakjae earthenwares were found that it indicates iron artifacts were manufactured in Bakjae Dynasty. From 6 iron sickles, called Beollat and Millet, samples were taken to examine mircostructures and from the results used iron material, shape-forging, steel-making process, heat treatment were investigated. From this late 4th century Bakjae dynasty iron manufacture profess was established. From the result, different treatment were used in different area of the sickles according to the its use. Beollats were quenched in blade and it was used for special purpose that require Intensity. Millats did not have special treatment that it was used to cut rice plant and plant. Used material was steel, steel was previously made by certain steel-making process. It is formed to produce iron ware and the area where intensity is required heat treatment was employed to make it strong. From the investigation it is derived that iron ware manufacture process in Bakjae dynasty is steel making$\rightarrow$shape-forging$\rightarrow$and heat treatment.

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Quality Characteristics and Reduced Sugar Conditions of Yanggaeng Prepared with Steamed Liriopis Tuber Extract Using Response Surface Methodology (반응표면분석법을 이용한 증숙 맥문동 첨가 양갱의 품질 특성 및 당류 저감화 조건 연구)

  • Park, Chanyoung;Park, So Hae;Kim, Won Baek;Hwang, Ji Hoe;Lee, Heeseob
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.2
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    • pp.229-236
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    • 2017
  • This study was performed to optimize yanggaeng processing conditions prepared with different amounts of steamed Liriopis tuber (SLT) extract, sugar, and agar powder using response surface methodology. The experimental conditions were designed according to the central composite design with 16 experimental points, including two replicates for three independent variables. The experimental data on physicochemical properties, textural properties, and sensory evaluation were fitted to various models, and the accuracy of the equations was analyzed by ANOVA. Among the responses, pH, water content, sugar content, L-value, browning index, chewiness, gumminess, and sensory properties of appearance, color, sweetness, hardness, texture, and overall acceptability showed significant correlation with contents of SLT, sugar, and agar powder. From these results, optimum formulation of yanggaeng was calculated as follows: SLT 35 mL, sugar 55.23 g, and agar powder 3.39 g.

Repetitive Electroacupuncture Alleviate Neuropathic Pain in Association with Suppressing Activation of Spinal Glial Cells (반복적인 전침 처치의 척수 교세포 활성 억제를 통한 신경병증성 통증 억제 효과)

  • Lee, Heun Joo;Jeong, Bo Eun;Song, Da Eun;Park, Min Young;Koo, Sungtae
    • Korean Journal of Acupuncture
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    • v.30 no.1
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    • pp.56-63
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    • 2013
  • Objectives : Effects of repetitive electroacupuncture(EA) on the pain behavior and activation of spinal glial cells were examined in the rat model of neuropathic pain. Methods : Twenty one adult male Sprague-Dawley rats were randomly assigned into 3 groups(control group, SP6 group, ST36+GB34 group). Neuropathic pain was induced by tight ligation of L5 spinal nerve. Mechanical and thermal hypersensitivity of hind paw were tested. Immunohistochemistry was performed in spinal cord L5/6 of all groups. EA was treated once in a day from the $5^{th}$ day after surgery. Results : EA treatments applied to ST36 and GB34 reduced significantly both of mechanical and thermal hypersensitivity after 3 times of treatment throughout the experiments. In the SP6 group, the analgesic effect was also shown after 7 times of treatment. Immunohistochemistry demonstrated inhibition of microglia and astrocyte activation in the spinal cord L5/6 dorsal horn in the ST36+GB34 group. Conclusions : The present results suggest that repetitive EA exert strong analgesic effect on neuropathic pain. These analgesic effects in neuropathic pain are associated with suppressing the activation of microglia and astrocyte.

Settlement Characteristics of Large Drilled Shafts Embedded in Bed Rocks (암반에 근입된 대구경 현장타설말뚝의 침하특성)

  • Hong Won-Pyo;Yea Geu-Guwen;Nam Jung-Man;Lee Jae-Ho
    • Journal of the Korean Geotechnical Society
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    • v.21 no.5
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    • pp.111-122
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    • 2005
  • The data on the pile load tests performed on 35 large drilled shafts are analyzed to investigate the load-settlement characteristics of large drilled shafts embedded in bed rocks. Generally, the settlement of large drilled shafts embedded in bed rocks is too small to determine the ultimated load with application of the regulation in design code for either the total settlement or the residual settlement. Therefore, to determine the yield load of large drilled shafts embedded in bed rocks, p(load)-logS (settlement) curve method, which has been proposed originally for the driven pile, was applied to the investigation on the data of the pile load tests. This technique shows that the yield load can be determined accurately and easily rather than other conventional techniques such as P-S, logp-logS, S-logt, and P-S curve methods. An empirical equation is proposed to represent the relationship between pile load and settlement before the yield loading condition. And the settlement of piles was related with the depth embedded in rock as well as rock properties. Based on the investigation on the data of pile load tests, the resonable regulations f3r both the total settlement and the residual settlement are proposed to determine the yield load of large drilled shafts embedded in bed rocks.

Storage and Acceptability of a Smoked Sebastes schlegeli Product (훈연처리에 의한 조피볼락의 저장성 및 기호도)

  • Lee, In-Sung;Kim, In-Cheol;Chae, Myoung-Hee;Chang, Hae-Choon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.11
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    • pp.1458-1464
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    • 2007
  • This study was conducted to develope a new type of ready-to-eat smoked Sebastes schlegeli product with high acceptability and extended shelf-life. A Sebastes schlegeli was salted at 4% salt concentration for 6 hr at $4^{\circ}C$. The cold smoking conditions for the salted Sebastes schlegeli consisted of drying for 2 hr at $22{\sim}24^{\circ}C$ followed by smoking for 2 hr at $22{\sim}24^{\circ}C$. The warm smoking conditions for the salted Sebastes schlegeli consisted of drying for 2 hr at $22{\sim}24^{\circ}C$, smoking for 2 hr at $22{\sim}24^{\circ}C$, and smoking again for 30 min at $47{\sim}50^{\circ}C$. The rancidity of the smoked Sebastes schlegeli did not change after 1 year storage at $-20^{\circ}C$ by monitoring the iodine value, peroxide value, and acid value. The number of viable cells in the cold and warm smoked samples were counted as $7.4{\times}10^5$ and $6.2{times}10^5$ CFU/g, respectively. Viable cells were not detected after 1 year of storage at $-20^{\circ}C$. The sensory evaluations of the processed Sebastes schlegeli showed that elastic texture increased with smoking as compared to with salting. There were no significant differences between cold and warm smoking in terms of sweetness, elastic texture, color, and smoke flavor. However, for overall acceptability, preference were in the oder of cold smoked, warm smoked, and salted.

Case studies of shallow marine investigations in Australia with advanced underwater seismic refraction (USR) (최신 수중 탄성파 굴절법(USR)을 이용한 호주의 천부해양탐사 사례연구)

  • Whiteley, Robert J.;Stewart, Simon B.
    • Geophysics and Geophysical Exploration
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    • v.11 no.1
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    • pp.34-40
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    • 2008
  • Underwater seismic refraction with advanced interpretation approaches makes important contributions to shallow marine exploration and geotechnical investigations in Australia's coastal areas. A series of case studies are presented to demonstrate the recent applications of continuous and static USR methods to river crossing and port infrastructure projects at various sites around Australia. In Sydney, static underwater seismic refraction (USR) with bottom-placed receivers and borehole seismic imaging assisted the development of improved geotechnical models that reduced construction risk for a tunnel crossing of the Lane Cove River. In Melbourne, combining conventional boomer reflection and continuous USR with near-bottom sources and receivers improved the definition of a buried, variably weathered basalt flow and assisted dredging assessment for navigation channel upgrades at Geelong Ports. Sand quality assessment with continuous USR and widely spaced borehole information assisted commercial decisions on available sand resources for the reclamation phase of development at the Port of Brisbane. Buried reefs and indurated layers occur in Australian coastal sediments with the characteristics of laterally limited, high velocity, cap layers within lower velocity materials. If these features are not recognised then significant error in depth determination to deeper refractors can occur. Application of advanced refraction inversion using wavefront eikonal tomography to continuous USR data obtained along the route of a proposed offshore pipeline near Fremantle allowed these layers and the underlying bedrock refractor to be accurately imaged. Static USR and the same interpretation approach was used to image the drowned granitic regolith beneath sediments and indurated layers in the northern area of Western Australia at a proposed new berthing site where deep piling was required. This allowed preferred piling sites to be identified, reducing overall pile lengths. USR can be expected to find increased application to shallow marine exploration and geotechnical investigations in Australia's coastal areas as economic growth continues and improved interpretation methods are developed.

On the Source Identification by Using the Sound Intensity Technique in the Radiated Acoustic Field from Complicated Vibro-acoustic Sources (음향 인텐시티 기법을 이용한 복잡한 진동-음향계의 방사 음장에 대한 음원 탐색에 관하여)

  • 강승천;이정권
    • The Journal of the Acoustical Society of Korea
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    • v.21 no.8
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    • pp.708-718
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    • 2002
  • In this paper, the problems in identifying the noise sources by using the sound intensity technique are dealt with for the general radiated near-field from vibro-acoustic sources. For this purpose, a three-dimensional model structure resembling the engine room of a car or heavy equipment is considered. Similar to the practical situations, the model contains many mutually coherent and incoherent noise sources distributed on the complicated surfaces. The sources are located on the narrow, connected, reflecting planes constructed with rigid boxes, of which a small clearance exists between the whole box structure and the reflecting bottom. The acoustic boundary element method is employed to calculate the acoustic intensity at the near-field surfaces and interior spaces. The effects of relative source phases, frequencies, and locations are investigated, from which the results are illustrated by the contour map, vector plot, and energy streamlines. It is clearly observed that the application of sound intensity technique to the reactive or reverberant field, e.g., scanning over the upper engine room as is usually practiced, can yield the detection of fake sources. For the precise result for such a field, the field reactivity should be checked a priori and the proper effort should be directed to reduce or improve the reactivity of sound field.