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http://dx.doi.org/10.15324/kjcls.2022.54.2.119

Antibacterial Effect of Various Fermentation Products and Identification of Differentially Expressed Genes of E.coli  

Heo, Jihye (Department of Biomedical Laboratory Science, Donggang University)
Publication Information
Korean Journal of Clinical Laboratory Science / v.54, no.2, 2022 , pp. 119-124 More about this Journal
Abstract
Pseudomonas aeruginosa (P. aeruginosa), Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) are typical opportunistic pathogens. Moreover, these bacteria are known to possess multidrug-resistant (MDR) properties. This study investigates the antimicrobial activity of six fermented products, which have varying efficacies against P. aeruginosa, E. coli, and S. aureus. To identify novel candidate genes, differential expression analysis was performed using an annealing control primer. In the disk diffusion method, Fig vinegar (FV) and Diospyros kaki Thunb vinegar (DTV) showed the greatest increase in inhibition compared to other fermented products, whereas fermented Korean traditional nature herb (FKTNH) had no antibacterial effect. This study identified down-regulation of Escherichia coli O157:H7 ompW gene for outer membrane protein W, whereas gene for synthetic construct Lao1 gene for L-amino acid oxidase were up-regulated in E. coli treated with 5% FV. Consuming fermented vinegar helps prevent bacterial infections. Especially, FV and DTV are potentially useful alternative natural products for multidrug resistance. Furthermore, both are expected to be used as effective natural antimicrobial agents, such as disinfectants.
Keywords
Differentially expressed genes; Escherichia coli; Fermentation; Fig vinegar;
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