• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.1
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• pp.1-6
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• 2008

Korditwo is a new orchardgrass(Dactylis glomerata L.) variety developed by the National Institute of Animal Science(NIAS) in 2005. To develope the new variety of orchardgrass, 5 superior clones, Dg8533, Dg9518, EDg9133, U8504 and U8519, were selected and polycrossed for seed production. That synthetic variety was designated newly as "Korditwo", and it's agronomic growth characteristics and forage productivity were examined at Suwon from 2001 to 2002, and regional yield trials were conducted in Suwon, Pyungchang, Youngju, Iksan, and Jeju from 2003 to 2005, respectively. "Korditwo" showed medium type growth habit in fall and spring and short in length of flag leaf and upper internode. Plant height of "Korditwo" was similar to that of standard variety, "Ambassador" and heading date was 4 days later than 14th May compared to Ambassador. Characteristics such as winter hardiness, lodging tolerance, disease resistance of "Korditwo" were stronger or better than those of Ambassador, specially in winter hardiness and lodging tolerance. "Korditwo" showed 10% higher dry matter yield(11.3 ton/ha) compared to Amabassador. Nutritive value was appeared to be similar in both varieties.

• Journal of the Korean Wood Science and Technology
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• v.41 no.3
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• pp.258-268
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• 2013

Wood-based products market is new growth engine industry of "Green growth policy". Korean government established a law, sustainable use of wood, for expanding wood and wood-based products market in 2012. This study was conducted to seek sustainable measures for conservation and management of valuable natural resources, offer expanding quality assurance services for wood-based products, and explore measures for expanding wood-based products labeling system. Through discussed domestic and overseas trends and literatures that concern wood-based products labeling system and investigated case studies that were presently operational. For expanding quality assurance services of wood-based products, Korea Forest Service operates a wood-based products labeling system in integrated management through variety awareness and specialist assessment for each product. This process will make enhanced wood-based products labeling system expanded gradually. To evolve into $3^{rd}$ party quality assurance services on maturity age of labeling system, public communication will help improve awareness of producer and consumer. appropriate incentive program will attract voluntary participation from woodbased product industry. Producers consider environmental ethics in their business and consumer should exercise their right to know.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.49-49
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• 2018

본 연구는 관상가치가 높아 조경 및 관상소재로 개발이 가능한 큰개관중의 전엽체 증식 및 포자체 형성에 적합한 배양조건을 구명하고자 수행되었다. 실험재료는 무가온 온실에서 채집한 포자를 기내에서 발아시켜 전엽체를 획득한 후 8주 간격으로 계대배양하여 실험에 사용하였다. 배지종류에 따른 전엽체의 기내 증식 및 형태형성의 영향을 알아보고자 배양된 전엽체 300mg을 메스로 다진 다음 농도를 1/4, 1/2, 1, 2배로 조절한 MS와 Knop배지에 8주간 배양하였다. 그 결과, 1MS배지에서 전엽체의 생체중이 5.5g으로 가장 많이 증가하였다. 1MS를 제외한 타 처리구는 생체중의 증가수준이 1.1-3.0g에 머물러 1MS배지보다 저조한 수준을 보였다. 현미경을 이용한 전엽체의 관찰결과, 1MS배지는 엽육의 색이 녹색으로 생육이 양호하였다. 전엽체의 증식이 가장 저조하였던, 2MS와 1/4MS는 생육의 저조뿐만 아니라 노화현상도 관찰되었다. 포자체 형성을 위한 최적의 토양조건을 알아보고자, 원예상토, 피트모스, 펄라이트 및 마사토의 비율을 5종류로 달리하여 배양토를 혼합하였다. 혼합된 토양은 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진하여 기내배양된 전엽체 1g을 증류수와 함께 10초간 분쇄한 다음 토양표면에 분주 후 10주간 재배하였다. 그 결과, 원예상토가 높은 비율로 첨가된 혼합조건에서 포자체의 형성이 우수하였다. 그중 원예상토와 마사토를 2:1(v:v)로 혼합한 토양, 원예상토와 펄라이트를 2:1(v:v)로 혼합한 토양에서 포트 당 각 357.0, 339.8개의 포자체가 형성되었다. 또한 형성된 포자체의 생육을 조사한 결과 원예상토와 마사토를 2:1(v:v)로 혼합한 토양에서 생체중, 엽수, 엽장, 엽폭, 근수, 근장 및 SPAD value 등의 생육수치가 우수하였다. 따라서 큰개관중의 전엽체 증식에 적합한 배지는 1MS로 판단되었으며, 포자체 대량생산을 위해서는 원예상토와 마사토를 2:1(v:v)로 혼합한 토양이 적합하다고 판단된다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.40-40
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• 2018

비고사리과(Lindsaeaceae) 버들일엽[Laxqgramme saliciblia (Makino) Makino]은 잎이 녹색의 단엽으로 버들처럼 보여 관상가치가 높아 조경소재로 이용되는 착생식물이다. 또한 식물구계학적 특정식물종 V급, 적색자료집 VU급, 국외반출 승인대상종에 수록된 식물로 생태학적 보전이 요구되며, 개체수도 적은 편으로 알려져 있다. 본 연구는 보전이 요구되는 버들일엽의 전엽체 증식 및 포자체 형성을 위한 기내 외 번식조건을 구명하고자 수행되었다. 서귀포시 상효동 일대에서 식물을 획득하였으며, 기내에서 포자를 발아시켜 획득한 전엽체를 8주 간격으로 계대배양하면서 실험재료를 확보하였다. 전엽체 증식에 적합한 배양배지를 선발하고자, 전엽체 300mg을 다진 후 배지종류와 농도를 달리한 배지(Knop, 1/4, 1/2 및 1MS)에 8주간 배양하였다. 배양실은 온도 $25{\pm}1.0^{\circ}C$, 광도 $30{\pm}1.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$(16/8h)로 조절되었다. 연구의 결과, 1/2MS배지에서 전엽체의 생체중이 5.8g으로 가장 많이 증가하였으며, 형태형성의 발달도 주걱형의 전엽체로 정상적으로 발달하였다. 기외 포자체 형성에 적합한 토양조건을 확인하고자, 인공토양(원예상토, 피트모스, 펄라이트 및 마사토)의 비율을 달리하여 5종류의 혼합토양을 조성하였다. 준비된 토양을 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진하고, 전엽체 1g을 10초간 분쇄한 다음 토양표면에 분주하여 16주간 재배하였다. 재배환경은 온도 $25{\pm}1.0^{\circ}C$, 광도 $43{\pm}2.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$(16/8h) 및 습도 $72{\pm}2.0%$로 조절되었다. 연구의 결과, 원예상토가 많은 비율로 혼합된 처리에서 포자체의 형성이 확인되었다. 원예상토와 펄라이트를 2:1(v:v)로 혼용한 토양, 원예상토와 마사토를 2:1(v:v)로 혼용한 토양, 원예상토 단용한 토양에서 각 28.5, 23.0, 22.3개로 조사되었다.

• Journal of Plant Biotechnology
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• v.41 no.3
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• pp.159-167
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• 2014

In eukaryotes, proteins that are secreted into ER are post-translationally modified by N-glycosylation, the patterns of which are significantly different between plant and animal cells. Biotechnology industry has already produced a number of therapeutic glycoproteins in plant cells. However, the aberrant glycosylation of therapeutic recombinant proteins in plant systems can cause immune problems in humans. Therefore, it is important to develop strategies for producing non-glycosylated forms to preserve biological activity and native conformation by a peptide: N-glycanase (PNGase). In this study, we try to isolate PNGase T gene from tomato, which can use as a platform plant for biotechnology industry. We isolated a cDNA (GenBank Accession number KM401550) from tomato leaves with 1,767 bp, which encoded a polypeptide of 588 amino acids with a predicted molecular mass of 65.8 kDa. We also investigated the expression patterns of PNGase T during fruit ripening of tomato. The transcripts of PNGase T, which were constitutively induced in tomato fruit from green stage, were significantly increased and reached a peak at orange stage. After which, those transcripts were continuously reduced. The expression pattern of PNGase T was coincided well with transcripts profiles of metacaspase gene, LeMCA, and senescence-related gene members of ACC synthase, LeACS2, LeACS4, and LeACS6, for ethylene biosynthesis during fruit ripening. These results suggest that PNGase T is involved in a de-glycosylation process associated with senescence and fruit ripening.

• KSBB Journal
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• v.20 no.6
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• pp.393-400
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• 2005

Hydrogen($H_2$) as a clean, and renewable energy carrier will be served an important role in the future energy economy. Several biological $H_2$ production processes are known and currently under development, ranging from direct bio-photolysis of water by green algae, indirect bio-photolysis by cyanobacteria including the separated two stage photolysis using the combination of green algae and photosynthetic microorganisms or green algae alone, dark anaerobic fermentation by fermentative bacteria, photo-fermentation by purple bacteria, and water gas shift reaction by photosynthetic or fermentative bacteria. In this paper, biological $H_2$ production processes, that are being explored in fundamental and applied research, are reviewed.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.1
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• pp.31-35
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• 2015

This experiment was carried out to breed a very early-maturing variety of Italian ryegrass (Lolium multiflorum Lam.) in the Grassland and Forage Crops Division, National Institute of Animal Science, RDA, Cheonan, Korea from 2012 to 2014. New variety "Greenfarm3ho" is a diploid variety with a green leaf color, a semi-erect growth habit in late autumn/fall, and an erect growth habit in early spring. With a heading date of April 27, Greenfarm3ho was categorized as an early-maturing variety. Compared with the "Greenfarm" control variety, Greenfarm3ho's flag-leaf width was narrower by 0.5 mm, its flag-leaf length was longer by 4.4 cm, and its plant length was longer by 3 cm; additionally, Greenfarm3ho's stem thickness was 0.1 mm thicker and it showed winter hardiness. The dry matter (DM) yield (8,976 kg/ha) of Greenfarm3ho was similar to that of Greenfarm. The in vitro dry matter digestibility (IVDMD), total digestible nutrient (TDN), and crude protein (CP) of Greenfarm3ho were 70.0%, 64.1%, and 1.0%, respectively, which are higher than those of Greenfarm by 2.9%, 2.0%, and 1.4%, respectively. The acid detergent fiber (ADF) and neutral detergent fiber (NDF) content of Greenfarm3ho were 31.4% and 53.3%, respectively, which are lower than those of Greenfarm by 2.5% and 3.0%, respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.1
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• pp.26-30
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• 2015

A new tall fescue variety (Festuca arundinacea Schreb.) named 'Greenmaster2ho' was developed by the National Institute of Animal Science, Rural Development Administration, in Cheonan, Korea from 2010 to 2014. For the synthetic seed production of this new variety, 5 superior clones were selected and polycrossed: 09XFa02, 09XFa03, 09XFa11, 09XFa13, and 09XFa14. The agronomic growth characteristics and forage production capability of the seeds were studied at Cheonan from 2010 onward, and regional trials were conducted in Cheonan, Hoengseong, Jeju, and Jinju from 2012 to 2014. Greenmaster2ho showed resistance to disease, persistence, and regrowth ability that were all enhanced when compared with Fawn. At 15,119 kg/ha, the dry matter (DM) yield of Greenmaster2ho was 29% higher than that of Fawn, but the nutritive value of both varieties as forage was similar. This study aimed to make a contribution to the vitalization of the Korean grassland industry by developing a new tall fescue variety with excellent environmental adaptability.

• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.1
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• pp.9-14
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• 2011

This experiment was carried out to breed a very early maturing variety of Italian ryegrass (Lolium multiflorum Lam.) in Grassland and Forage Crops Division, National Institute of Animal Science, RDA, Cheonan from 2009 to 2010. A new variety, 'Green farm' is a diploid variety with green in leaf color and has erect and semi-erect growth habit in late autumn and early spring, respectively. 'Green farm' was on 28 April in heading date as a early-maturing variety. 'Green farm' was also wider by 0.4 mm in flag leaf width, longer by 0.8 cm in flag leaf length and shorter by 5 cm in plant height than those of control variety, 'Florida 80', respectively. 'Green farm' was thicker in stem thickness and stronger in winter hardness than those of the 'Florida 80'. Dry matter (DM) yield (11,790 kg/ha) of 'Green farm' was similar to that of 'Florida 80'. In vitro dry matter digestibility (IVDMD), total digestible nutrient (TDN) and crude protein (CP) of 'Green farm' were 68.7, 63.3 and 10.3% which are 1.9, 1.7 and 0.6% higher than those of 'Florida 80', respectively. Acid detergent fiber (ADF) and neutral detergent fiber (NDF) of 'Green farm' were 32.5 and 54.6% which are 2.2 and 4.3% lower than those of the 'Florida 80', respectively.

• Journal of The Korean Society of Grassland and Forage Science
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• v.33 no.1
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• pp.10-14
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• 2013

This experiment was carried out to breed an early maturing variety of Italian ryegrass (Lolium multiflorum Lam.) in the Grassland and Forage Crops Division, National Institute of Animal Science, RDA, Cheonan from 2009 to 2011. A new variety, 'Green farm II' is a diploid with green leaves and has a semi-erect growth habit in late autumn and erect growth habit in early spring, respectively. 'Green farm II' was on the $28^{th}$ of April in the heading date as an early-maturing variety. 'Green farm II' was also wider by 0.6 mm in flag leaf width, shorter by 1 cm in flag leaf length and shorter by 1 cm in plant length than those of the control variety, 'Florida 80', respectively. 'Green farm II' was thicker by 0.7 mm in stem thickness and strong in winter hardness. Dry matter (DM) yield (11,452 kg/ha) of 'Green farm II' was similar to that of 'Florida 80'. In vitro dry matter digestibility (IVDMD), total digestible nutrients (TDN), and relative feed value (RFV) of 'Green farm II' were 70.6%, 63.9%, and 114% higher than those of 'Florida 80', respectively. Acid detergent fiber (ADF) and neutral detergent fiber (NDF) of 'Green farm II' were 31.7% and 52.6% which were 3.5% and 6.4% lower than those of 'Florida 80', respectively.