• Title/Summary/Keyword: 노화촉진시험

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Occurrence and Influence of acid Leachate by Pyrite in Underground Rocks of Road Construction Field in the Miryang Area (밀양지역 도로건설 현장 지반암석내 분포하는 황철석에 의한 산성침출수 발생과 영향)

  • Chae, Sun Hee;Jeong, Chan Ho;Lee, Yu Jin;Lee, Yong Cheon;Shin, Sang Sik;Park, Jun Sik;Ou, Song Min
    • The Journal of Engineering Geology
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    • v.28 no.3
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    • pp.501-512
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    • 2018
  • The acid leachate derived from the sulfide mineral such as pyrite can cause problems such as aging of infrastructure and environment contamination around the civil construction site. The purpose of this study is to assess the environmental effect of an acid leachate derived from pyrite in the Miryang area under road construction. In this study, 13 samples of situ core were used for the net acid generation (NAG) experiment. The chemical composition including pH, oxidation and reduction potential (ORP) and electrical conductance of water samples produced from the NAG test was analyzed. In additional, five polished thin sections of rock cores were made for electro microprobe analyses. In the results of the NAG tests, 7 samples showed lower values than pH 3.5. It strongly indicated that these areas are under the environmental and infrastructure damage by the acid leachate. The chemical type of the 7 samples was classified as the $Fe(Ca)-SO_4$ type, which is totally a different type compared to general groundwater. The concentration of total sulfur ranges from 0.004% to 12.5%. 6 rock samples are plotted on a potentially acid forming zone in the relation diagram between the total sulfide and NAG-pH. In conclusion, it is suggested that a protection method against an environmental demage and an infrastructure corrosions by the acid leachate should be prepared in all of areas under a road construction.

A Study on the Testing of Weather Resistance of Anti-corrosion Wax Covering Agents in the Locomotive at Jangdan Station of Gyeongui Line (경의선장단역증기기관차의 부식방지를 위한 왁스계 피복제 내후성 시험 연구)

  • Song, Won-Joon;Kim, Mi-Hyun;Ahn, Byong-Chan;Jeong, Hee-Don
    • Journal of Conservation Science
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    • v.25 no.4
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    • pp.373-381
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    • 2009
  • To preserve steel cultural properties on display outdoors, the surface of these relics is processed with a covering treatment, which is a typical anti-corrosion method. Since the registered cultural heritage of Korea No. 78 Locomotive at Jangdan Station of Gyeongui Line will be treated by preservation process and displayed in the open outdoor area, effective surface covering agents were selected through testing to prevent the corrosion of the locomotive. The mixed wax are based on two types of microcrystalline wax which is one of regular surface covering agents for locomotives and bee‘s wax. For the weather resistance test, 3 types of wax were applied to each specimen of the locomotive and the specimens were tested in the Xenon-Arc Accelerated Weathering Tester for 1000 hours in accordance with the ISO 4892-1981 criteria and the test results were compared. As a result, on the surface of the specimen coated with the bee's wax, a whitening event appears after 400 hours which causes aging, and the specimens coated by the microcrystalline wax showed signs of a whitening event after 800 hours. The moisture contact angle with the microcrystalline wax coating was also less than with the bee's wax. Therefore, it was found that the high temperature microcrystalline wax is the most effective coating agent in terms of weather resistance and moisture blocking capability and the high temperature microcrystalline wax was adopted for coating the surface of the locomotive.

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Isolation and Identification of the Antagonistic Microorganisms Against Streptococous spp. Causing Dental Caries in Korean Soy Sauce (한국재래간장으로부터 구강질환 방제균의 선발 및 동정)

  • 엄수정;이여진;김진락;이은탁;김상달
    • Journal of Life Science
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    • v.13 no.4
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    • pp.535-540
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    • 2003
  • The antagonistic microorganisms against Streptococcus sanguis, S. salivarius and S. mutans causing the dental caries of oral diseases were isolated from Korean traditional soy sauce. Twenty five strains were isolated by pairing culture, paper disc culture and dual culture methods. The isolate NG 06 strain was observed with various cultural and physiological test, and $Biolog^{(R)}$ Bacterial Identification System. The strain was identified as Bacillus racemilacticus. The isolate NG 16 strain was confirmed to Gram-positive, rods, endospore production, utilization of melibiose, casein hydrolysis and starch hydrolysis. Also the second strain NG 16 was identified as $\beta$. amyloliquefaciens.

Dry-heat Treatment Effect for Seed Longevity Prediction in Rice Germplasm (벼 유전자원의 저장수명 예측을 위한 건열처리 효과)

  • Na, Young-Wang;Baek, Hyung-Jin;Choi, Yu-Mi;Lee, Sok-Young;Lee, Jung-Ro;Chung, Jong-Wook;Park, Yong-Jin;Kim, Seok-Hyeon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.3
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    • pp.230-238
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    • 2014
  • The purpose of this study was to develop the cost-effective and efficiency seed longevity prediction method of rice (Oryza sativa L.) germplasm for viability monitoring. To find an optimum predicting method for rice seed longevity at genebank, an accelerated ageing (AA) test, a controlled deterioration (CD) test and a dry-heat treatment (DHT) were conducted to the four groups of rice germplasm based on ecotype, such as Indica, Japonica, Javanica and Tongil type. Among the three artificial aging treatments, the dry-heat treatment of 36 hours at $90^{\circ}C$ is suggested as a routine predictive test method of rice germplasm longevity at a genebank. The distribution of germination rate on 3,066 accessions which conserved 26.5 years at $4^{\circ}C$ showed similar trend with the result of distribution by dry-heat treatment at $90^{\circ}C$ on 36 hours using 106 accessions of rice selected samples which composed four ecotype groups. The results show that the dry-heat treatment affect not only predicting the rice seed longevity but also determining effective interval for monitoring germination of rice germplasm in genebanks.

Anti-wrinkle Effect by Ginsenoside Rg3 Derived from Ginseng (인삼유래 Ginsenoside Rg3에 의한 항-주름 효과)

  • 김성우;정지헌;조병기
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.30 no.2
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    • pp.221-225
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    • 2004
  • The root of Panax ginseng C. A. Meyer has been used as a traditional anti-aging and anti-wrinkle agent in the Orient. However, it is still unknown which component of ginseng is effective at suppressing wrinkle formation. Recently at least twenty ginsenosides regarded as the main active ingredients of ginseng have been isolated. Among them, we examined the effect of ginsenoside Rg3 on dermal ECM metabolism to elucidate the mechanism of anti-wrinkle by ginseng. In our study, to investigate the anti-wrinkle effect of the ginsenoside Rg3, ECM component and growth factor in dennis were evaluated by ELISA assay. Ginsenoside Rg3 was found to stimulate type I procollagen and fibronectin (FN) biosynthesis in a dose-dependent manner in normal human fibroblast culture (p < 0.05, n =3), and dose-dependently enhance TGF- ${\beta}$1 level (p < 0.05, n =3). In RT-PCR analysis mRNA level of c-Jun, a member of AP-1 transcription factor, was reduced by ginsenoside Rg3 in normal human fibroblast culture. These results indicate that ginsenoside Rg3 stimulates type I collagen and FN synthesis through the changes of TGF - ${\beta}$1 and AP-1 expression in fibroblasts.

New Functional Properties of Passion Fruit Extract on Skin (패션 프룻 추출물이 피부에 미치는 새로운 기능적 효과)

  • Jeong, Mi Suk;Kim, Soon-Rae;Han, Chang Woo;Kim, Hyeon Jin;Jang, Se Bok
    • Journal of Life Science
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    • v.32 no.2
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    • pp.101-107
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    • 2022
  • In this research, the anti-inflammatory, antioxidant, antiaging, and skin whitening properties of pulp and seed extracts of passion fruit were studied. The result of the primary skin irritation test using a skin-attached patch determined the skin irritation index to be 0.00 for the passion fruit extract. In addition, RAW 264.7 macrophages produce NO by stimulation of lipopolysaccharides, and the application of extracts to this resulted in significantly lower NOs, confirming the excellent anti-inflammatory properties of passion fruit extracts. The 2,2-diphenyl-1-picrylhydrazyl test further confirmed that the passion fruit extract exhibits a good 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate radical scavenging ability of 5.11% and strong antioxidant properties. The presence of collagen type I in the skin is a measure of aging and various skin diseases. The results obtained from the analysis of the activity of human procollagen I alpha 1 confirmed that the passion fruit extract reduces the synthesis of procollagen. In addition, the skin whitening property of the passion fruit extract was confirmed by the melanin inhibition test, and a sample was obtained that contained more than 2% of arbutin, a whitening agent approved by the Ministry of Food and Drug Safety, which is generally present in the form of a white powder and is used as a functional ingredient. This confirms that the whitening efficacy of the passion fruit extract obtained from nature contributes to the development of functional raw materials for cosmetics and food.

Studies on Varietal Differences in Growth, Nodulation and Nitrogen Fixation in Soybeans, Glycine max (L.) Merrill. III. Relationships between Nitrogen Fixation Activity and Nitrogen Content of Plant Organs during Grain Filling Period (콩의 생육, 근류형성, 질소고정에 있어서 품종간 차이 III. 등숙기간중 식물체 각 기관 질소함량의 경시적 변동과 질소고정활성과의 관계)

  • Kim, Seok-Dong;Ishizuka, Junji;Park, Rae-Kyeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.2
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    • pp.161-168
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    • 1988
  • Five soybean varieties of two early maturing ; Karikei 73 and ss 79168, and three late maturing ; Tohoku 76, Baegunkong and Jangbaegkong were used and evaluated in the study. Of the varieties examined, Karikei 73 was characterized by the delayed leaf senescence. To investigate the periodical trends of nitrogen in plant organs and their roles to the nitrogen fixing activity of root nodules, the concentrations of nitrogen in plant organs during the grain filling period were measured. High positive correlation was recognized between the amount of nitrogen in plant parts and p-ARA, suggesting the higher the activity of nitrogen fixation the greater the amount of nitrogen in plant. But the relationship between the concentration of nitrogen in plant and s-ARA was positive at R4. but negative at R6. This was inferred that the high concentration of nitrogen at R4 gave accelerating effects on s-ARA by increasing the activity of photosynthesis in leaf-blades, while the reduced activity of photosynthesis in leaf-blades at R6 resulted in continued degradation of nitrogenous compounds in plant, and then high concentration of nitrogen in plants brought reduced supply of sugars to nodules.

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Natural Dyeing Characteristics of Korean Traditional Paper (전통 한지의 천연염색 특성)

  • Choi, Tae-Ho
    • Journal of the Korean Wood Science and Technology
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    • v.34 no.3
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    • pp.90-98
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    • 2006
  • The purpose of this study was to estimate natural dyeing properties of Korean traditional paper (Hanji). Korean traditional paper, which made from bleached bast fiber pulp of Broussonetia kazinoki was used as base paper. As dyestuffs, hot water extractives of Phellodendron amurense bark, Rosa multiflora leaf and stalk, and Rubia cordifolia root and methanol extractives of Lithospermum erytrorhizon root were used. $Al_2(SO_4)_3$ and $Fe(OH)(CH_3COO)_2$ 0.5% solution were used as mordant. Soybean juice (10%), chitosan (0.1%), and skim milk (2%) solutions were used as auxiliary dyeing agents. Accelerated aging treatments of natural dyed Korean traditional papers were undergone at following conditions: exposure temperature, $80^{\circ}C$ relative humidity, 60%; wavelength, 340 nm; UV irradiance, $0.67W/m^2/nm$ exposure time, 24, 48, and 72 hours. Colors, color difference, and color fastness were examined for estimation of natural dyeing characteristics. The auxiliary dyeing agents treated Korean traditional papers were more superior the dyeing effectiveness about dyestuffs than untreated Korean traditional papers. The dyeing effectiveness of soybean juice treated Korean traditional paper was superior to the others. The color fastness of Korean traditional paper, which was dyed with Rubia cordifolia root extractives, was most inferior to the others.

Studies on the Sugar Analysis and Biological Activity of Sap from Juglans mandshurica Maxim. (가래나무 수액의 당성분 분석 및 생리활성 연구)

  • Kim, Dae-Wook;Choi, Mi-Na;Lee, Min-Sung;Jung, Hae-Suk;Byeon, Jun-Gi;Kim, Yeong-Su
    • Journal of Chitin and Chitosan
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    • v.23 no.4
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    • pp.262-266
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    • 2018
  • Even though the saps have been consumed as beverage in Korea, however a little research has been conducted on the chemical composition. We determined free sugars, mineral in the sap from Juglans mandshurica Maxim. collected from BongHwa region. The contents of glucose, sucrose, and fructose in the sap from J. mandshurica were 0.15, 2.73, and 0.09%, respectively. The prominent minerals in the sap from J. mandshurica were calcium and potassium. The contents of Ca, K, Mg, Fe, and Mn of sap from J. mandshurica were 0.61, 0.57, 0.12, 0.002, and 0.014 ppm, respectively. The sap from J. mandshurica show inhibitory effect on elastase. And real-time RT-PCR showed that sap from J. mandshurica increased mRNA level of AQP3 and HAS2 gene and increased hyaluronic acid production in HaCaT cells. These results indicated that the sap from J. mandshurica can potentially be used for developing cosmetic ingredient for skin moisturizing and anti-aging.

The change of grain quality and starch assimilation of rice under future climate conditions according to RCP 8.5 scenario (RCP 8.5 시나리오에 따른 미래 기후조건에서 벼의 품질 및 전분 동화 특성 변화)

  • Sang, Wan-Gyu;Cho, Hyeoun-Suk;Kim, Jun-Hwan;Shin, Pyong;Baek, Jae-Kyeong;Lee, Yun-Ho;Cho, Jeong-Il;Seo, Myung-Chul
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.20 no.4
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    • pp.296-304
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    • 2018
  • The objective of this study was to analyze the impact of climate change on rice yield and quality. Experiments were conducted using SPAR(Soil-Plant-Atmosphere-Research) chambers, which was designed to create virtual future climate conditions, in the National Institute of Crop Science, Jeonju, Korea, in 2016. In the future climate conditions($+2.8^{\circ}C$ temp, 580 ppm $CO_2$) of year 2051~2060 according to RCP 8.5 scenario, elevated temperature and $CO_2$ accelerated the heading date by about five days than the present climate conditions, resulted in a high temperature environment during grain filling stage. Rice yield decreased sharply in the future climate conditions due to the high temperature induced poor ripening. And the spikelet numbers, ripening ratio, and 1000-grain weight of brown rice were significantly decreased compared to control. The rice grain quality was also decreased sharply, especially due to the increased immature grains. In the future climate conditions, expression of starch biosynthesis-related genes such as granule-bound starch synthase(GBSSI, GBSSII, SSIIa, SSIIb, SSIIIa), starch branching enzyme(BEIIb) and ADP-glucose pyrophosphorylase(AGPS1, AGPS2, AGPL2) were repressed in developing seeds, whereas starch degradation related genes such as ${\alpha}-amylase$(Amy1C, Amy3D, Amy3E) were induced. These results suggest that the reduction in yield and quality of rice in the future climate conditions is likely caused mainly by the poor grain filling by high temperature. Therefore, it is suggested to develop tolerant cultivars to high temperature during grain filling period and a new cropping system in order to ensure a high quality of rice in the future climate conditions.