Browse > Article

Anti-wrinkle Effect by Ginsenoside Rg3 Derived from Ginseng  

김성우 (㈜코리아나화장품 피부과학연구센터)
정지헌 (㈜코리아나화장품 피부과학연구센터)
조병기 (㈜코리아나화장품 피부과학연구센터)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.30, no.2, 2004 , pp. 221-225 More about this Journal
Abstract
The root of Panax ginseng C. A. Meyer has been used as a traditional anti-aging and anti-wrinkle agent in the Orient. However, it is still unknown which component of ginseng is effective at suppressing wrinkle formation. Recently at least twenty ginsenosides regarded as the main active ingredients of ginseng have been isolated. Among them, we examined the effect of ginsenoside Rg3 on dermal ECM metabolism to elucidate the mechanism of anti-wrinkle by ginseng. In our study, to investigate the anti-wrinkle effect of the ginsenoside Rg3, ECM component and growth factor in dennis were evaluated by ELISA assay. Ginsenoside Rg3 was found to stimulate type I procollagen and fibronectin (FN) biosynthesis in a dose-dependent manner in normal human fibroblast culture (p < 0.05, n =3), and dose-dependently enhance TGF- ${\beta}$1 level (p < 0.05, n =3). In RT-PCR analysis mRNA level of c-Jun, a member of AP-1 transcription factor, was reduced by ginsenoside Rg3 in normal human fibroblast culture. These results indicate that ginsenoside Rg3 stimulates type I collagen and FN synthesis through the changes of TGF - ${\beta}$1 and AP-1 expression in fibroblasts.
Keywords
ginsenoside Rg3; anti-wrinkle; extracellular matrix; TGF-${\beta}$1;
Citations & Related Records
연도 인용수 순위
  • Reference
1 L. Rittie and G. J. Fisher, UV-light-induced signal cascades and skin aging, Ageing Res Rev, 1(4), 705 (2002)
2 J. Massague, The transforming growth factor-beta family, Annu Rev Cell Biol, 6, 597 (1990)
3 W. Chen, A. H. Borchers, Z. Dong, M. B. Powell, and G. T. Bowden, UVB irradiation-induced activator protein-1 activation correlates with increased c-fos gene expression in a human keratinocyte cell line, J. Biol Chem, 273(48), 32176 (1998)
4 G. Liau and L. M Chan, Regulation of extracellular matrix RNA levels in cultured smooth muscle cells. Relationship to cellular quiescence, J. Biol Chem, 264(17), 10315 (1989)   PUBMED
5 T. A. Mustoe, G. F. Pierce, A Thomason, P. Gramates, M. B. Sporn, and T. F. Deuel, Accelerated healing of incisional wounds in rats induced by transforming growth factor-beta, Science, 237(4820), 1333 (1987)
6 J. H. Chung, S. Kang, J. Varani, J. Lin, G. J. Fisher, and J. J. Voorhees, Decreased extracellular-signal-regulated kinase and increased stress-activated MAP kinase activities in aged human skin in vivo, J. Invest Dermatol, 115(2), 177 (2000)
7 M. Karin, Z. Liu, and E. Zandi, AP-1 function and regulation, Curr Opin Cell Biol, 9(2), 240 (1997)   DOI   ScienceOn
8 G. J. Fisher and J. J. Voorhees, Molecular mechanisms of photoaging and its prevention by retinoic acid: ultraviolet irradiation induces MAP kinase signal transduction cascades that induce Ap-1-regulated matrix metalloproteinases that degrade human skin in vivo, J. Investig Dermatol Symp Proc, 3(1), 61 (1998)
9 G. J. Fisher, H. S. Talwar, J. Lin, P. Lin, F. McPhillips, Z. Wang, X. Li, Y. Wan, S. Kang, and J. J. Voorhees, Retinoic acid inhibits induction of c-Jun protein by ultraviolet radiation that occurs subsequent to activation of mitogen-activated protein kinase pathways in human skin in vivo, J. Clin Invest, 101(6), 1432 (1998)
10 P. Angel and M Karin, The role of Jun, Fos and the AP-l complex in cell-proliferation and transformation, Biochim Biophys Acta, 1072(2-3), 129 (1991)
11 D. Oda, A. M. Gown, J. S. Vande Berg, and R. Stern, Instability of the myofibroblast phenotype in culture, Exp Mol Pathol, 52(2), 221 (1990)