• Journal of Plant Biotechnology
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• v.29 no.4
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• pp.247-252
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• 2002

The combination of radiation technique with an in vitro culture system was initiated to develop salt tolerant rice. We established an in vitro culture system to select tolerant lines against salt stress. NaCl tolerant cell lines were selected from the callus irradiated with gamma ray on N$_{6}$ medium with 1.5% NaCl and 2 mg/L 2,4-D. Regenerants (M$_1$) were obtained from the tolerant callus which was cultured for 30 days auxin-free medium. The M$_2$seeds were harvested from M$_1$plants on an individual plant basis. Thirty seedlings from each 450 M$_2$lines were transplanted in a field and total 5,000 M$_3$lines were harvested with an average 90 percent of fertile grain. M$_3$lines were utilized for selection of salt tolerance. Salinity-tolerant lines (225) were selected among 5,000 M$_3$lines. Of the 225 lines tested, the morphological traits of two lines (120-10 and -11) were far superior to control (Donagjinbyeo) in agromomic traits such as plant height, root length and no. of roots. Control and tolerant lines were analyzed by RAPD markers. Three polymorphic bands were presented in only tolerant lines, demonstrating a genetic difference between control and the tolerant lines. Such tolerant lines could be used as genetic resources to improve salt tolerance.e.

• Korean Journal of Breeding Science
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• v.51 no.4
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• pp.318-325
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• 2019

Salt stress is a significant factor limiting growth and productivity in crops. However, little is known about the response and resistance mechanism to salt stress in maize. The objective of this research was to develop an enhanced salt-tolerant silage maize by mutagenesis with gamma radiation. To generate gamma radiation-induced salt-tolerant silage maize, we irradiated a KS140 inbred line with 100 Gy gamma rays. Salt tolerance was determined by evaluating plant growth, morphological changes, and gene expression under NaCl stress. We screened 10 salt-tolerant maize inbred lines from 2,248 M₂ mutant populations and selected a line showing better growth under salt stress conditions. The selected 140RS516 mutant exhibited improved seed germination and plant growth when compared with the wild-type under salt stress conditions. Enhanced salt tolerance of the 140RS516 mutant was attributed to higher stomatal conductance and proline content. Using whole-genome re-sequencing analysis, a total of 328 single nucleotide polymorphisms and insertions or deletions were identified in the 140RS516 mutant. We found that the expression of the genes involved in salt stress tolerance, ABP9, CIPK21, and CIPK31, was increased by salt stress in the 140RS516 mutant. Our results suggest that the 140RS516 mutant induced by gamma rays could be a good material for developing cultivars with salt tolerance in maize.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.6
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• pp.447-453
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• 2009

Vegetation distribution and soil salinity were surveyed on the conservation plot in the Daeho reclaimed tidal land, in where the plants species distribution was more various than a periodically inundated tidal flat and the early stage of reclamation. According to the soil salinity where the vegetation patches were occurred, the mono patches of Salicornia europaea, Suaeda maritima, and Suaeda glauca were distributed in the average range of 31.05 dS/m in soil salinity, the mixed patches of them were distributed in the average range of 42.75 dS/m. Therefore, Salicornia europaea, Suaeda maritima, and Suaeda glauca showed strong salt tolerance. The mono patches of Aster tripolium, Sonchus brachyotus, and Scirpus planiculm were distributed in the range of 11.73 dS/m in soil salinity, and the mixed patches were distributed in the average range of 9.43 dS/m. Therefore Aster tripolium, Sonchus brachyotus, and Scirpus planiculmis showed moderate salt tolerance. The mono patches of Imperata cylindrica, Trifolium pratense, Miscanthus sinensis, Setaria viridis, and Trisetum bifidum were distributed in the range of 2.42 dS/m in soil salinity. These species showed characteristics of glycophytes with weak salt tolerance. The distribution of vegetation patches was influenced by the soil salinity as pioneer halophytes patches occurred at higher soil salinity zone than facultative halophytes patches, glycophytes patches occurred at lower soil salinity zone than facultative halophytes. These results suggested that occurrence of plant species and plant distribution type might be useful index to evaluate the soil salinity and desalinization in the reclaimed land of the midwest coastal area of Korea.

• Applied Biological Chemistry
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• v.20 no.2
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• pp.221-227
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• 1977

Contents of proline and chloride in halophytes were $80-1700\;{\mu}g/gfw$ and 0.13-0.45 mM/gfw respectively. The content of proline was inversely proportional to that of chloride. Rhizomes of Phargmites communis Trin, a halophyte, were grown in non-saline medium and then taken to saline treatment for one or two weeks. Growth of P. communis was inhibited when salinized with 0.25M NaCl. Total nitrogen decreased and alcohol soluble nitrogen and proline increased when growth was retarded. The quantity of Fraction 1 protein decreased at 0.25M NaCl treatment. The accumulation of proline at high concentration in P. communis suggested that it might play a role in osmotic adjustment.

• Horticultural Science & Technology
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• v.19 no.3
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• pp.342-347
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• 2001

The experiment was carried out to examine the effect of culture media and seawater on growth and mineral concentrations in Glasswort (Salicornia herbacea). Glassworts were grown in a greenhouse with 0, 10, 30 and 90% seawater and with or without nutrient media. The salinity tolerance affected on shoot height and Na, K and Ca concentrations significantly. The shoot height was increased with 10 and 30% seawater concentrations. At the higher seawater concentrations, more Na accumulated in a shoot was obserbed. The application of seawater concentration was effective on increasing Na, Mg, and Fe, concentrations and green leaf area rate and on decreasing K concentration. K/Na ratio of shoot was decreased at high seawater concentration, whereas Na concentrations was lowered at a low seawater concentrations. The mineral concentrations were changed according to the growing season. Na, K and Mg were high in June and Ca and P in September, respectively.

• The Korean Journal of Ecology
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• v.25 no.2
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• pp.101-107
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• 2002

Three species of Chenopodiaceae, i.e. Suaeda japonica, Salicomia herbacea, Beta vulgaris var. cicla, were investigated to compare the physiological characteristics through inoic balances and osmoregulations under different environmental salt gradients. Plats were harvested in two weeks from treatments with salt gradients (0, 50, 100, 200 and 400 mM NaCl) and mineral nutrition gradients(1/1, 1/5, 1/10 dilutions of Hoagland solution). Plants were analyzed for growth responses, ionic balances, osmolalities, conductivities, glycinebetaine and proline contents quantitatively. Three plants of Chenopodiaceae accumulated slats into tissues unlike some salt sensitive species, and showed unique adaptation patterns to overcome saline environments, i.e. strong growth stimulation for Salicomia herbacea, growth negative tolerance for Suaeda japonica, and growth positive tolerance for Beta vulgaris var. cicla. The absorption of inorganic Ca/sup 2+/ ions was inhibited remarkably due to the excess uptake of Na+ with increasing salinity. The K+ content in plants was significantly reduced with increasing salinity. Total nitrogen content was reduced as mineral nutritions and salinity increased. Conductivity and osmolality increased with increasing salinity regardless of mineral nutritions. The ranges of glycinebetaine and proline contents were 0.2∼2.5 μM/g plant water and 0.1∼0.6μM/g plant water, respectively.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.10
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• pp.687-693
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• 2013

Coastal sand dunes have been seriously damaged caused by the development thoughtless for the environment and coastal erosion and destruction due to artificial structures like coast roads and breakwater. Hereupon, in this study we made a library of rhizobacteria that have the plant growth-promoting ability for plant rhizosphere of indigenous plants inhabiting in a coastal sand dune as well as the strong tolerance to salt, and evaluated the plant growth-promoting ability of these strains. Furthermore, we evaluated the effect of rhizobacteria on the growth rate of saline tolerant plants in sandy soil; selected out the most useful micro-organism for the restoration of a damaged sand dune. The effect of inoculation of strains selected from the first experiment on the growth of Peucedanum japonicum and Arundo donaxes planted in a coastal sand dune was evaluated. As a result, Bacillus aerius MH1RS1 had plant growth promoting activities: indole acetic acid (IAA) production, siderophores and 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase) activity, and also had a salinity tolerance. Also, in case of Peucedanum japonicum, the length of stems and weights of roots were enhanced by the inoculation of B. aerius MH1RS1. Fresh weights of stems and weights of roots in experimental group were, in particular, increased by 25% comparing with the control group. For an Arundo donax in experimental group, plant length increased by 18%, and weight of roots by 20% which is significant.

• Journal of Bio-Environment Control
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• v.21 no.4
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• pp.478-484
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• 2012

This study was carried out to investigate the plant growth and ion absorbance balance of various native plants affected by the NaCl concentration (0, 100, 200, 300 mM). Carex blepharicarpa, Carex lenta, Carex matsumarae, Carex sendaica, Iris pseudacorus L., Sedum oryzifolium Makino, Sedum polytrichoides Hemsl., and Typha angustifolia L. were used in this experiment. Carex blepharicarpa, Carex lenta, Carex matsumarae, and Iris pseudacorus L. were tolerant of salinity at the NaCl concentration of 200 mM. The root growth of Carex sendaica and Typha angustifolia L. was suppressed at the NaCl concentration of 100 mM, expecially the root growth responded more sensitively than the upper growth to salinity. The K absorbance of Carex sendaica decreased according to the NaCl application, and the Na/K rate value was 3 at the NaCl concentration of 300 mM. The K, Ca, and Mg absorbance of Typha angustifolia L. decreased at the NaCl concentration of 200~300 mM, and the Na/K rate value was 0.8 at the NaCl concentration of 300 mM. The plant growth of Sedum oryzifolium Makino and Sedum polytrichoides Hemsl. was suppressed at the NaCl concentration of 100~200 mM. The K, Ca, and Mg absorbance of Sedum oryzifolium Makino decreased at the NaCl concentration of 200~300 mM, and Sedum polytrichoides Hemsl. was unaffected by the NaCl application. The Na/K value was 1 in both plants. Therefore, Carex blepharicarpa, Carex lenta, Carex matsumarae, and Iris pseudacorus L. were tolerant plants of salinity at the NaCl concentration of 200 mM considering the plant growth and ion absorbance balance. Especially, the Carex plants were expected to expanding use by the proven tolerance of salinity. The root growth of Carex sendaica, Sedum oryzifolium Makino, Sedum polytrichoides Hemsl., and Typha angustifolia L., was suppressed at the NaCl concentration of 100 mM, but there was no distinct tendency of ion absorbance in leaves according to the NaCl application.

• Journal of Plant Biotechnology
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• v.39 no.4
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• pp.253-260
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• 2012

On the basis of the reported agriculturally valuable phenotypes resulted from ectopic overexpression of Arabidopsis vacuolar $H^+$-PPase (AVP1), we generated the Chinese cabbage lines expressing AVP1 which then subjected to salt stress to determine the AVP1 expression if it consistently confers the capability for increasing biomass and enhancing tolerance to salinity in other species. Collectively, here we demonstrate that the transgenic young plants show more vigorous growth and higher tolerance to salt stress than wild-type ones. Increased biomass phenotype by AVP1 expression was supported by comparing fresh and dry weights of transgenic and wild type plants grown under normal condition, while higher salt tolerance trait was confirmed by tracing the kinetics of photosystem II quantum yield and DAB-staining under gradually intensified salt stress induced by MS salt or NaCl, followed by normal condition.

• Journal of Plant Biology
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• v.23 no.1
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• pp.27-33
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• 1980

The germination character and the salt tolerance of seeds collected in a salt reclaimed area were studied. Twenty species of seeds out of 30 species collected were germinated under natural or continuous light conditions but only 16 species under dark condition. Germination percentage of seeds under dark was more decreased than those under other two conditions and speed of germination was accelerated at high temperature. It is clear that almost all the wild weeds were the light germinated seeds. The higher concentration of NaCl for germination inhibited to make the lower percentage and rate of germination. The germination curves of seeds treated with NaCl solution were classified into 3 different curves; a steeper, a parabola and intermediate. The critical concentrations of NaCl for germination were 2.3% for Brassica napus, 1.8% for Echinochloa hispidula, 1.5% for Setaria lutescens, 1.3% for Aster koraiensis, 0.7% for Bromus japonicus and 0.6% for Glyceria acutiflora.