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http://dx.doi.org/10.5010/JPB.2002.29.4.247

In Vitro Selection and Characterizations of Gamma Radiation-Induced Salt Tolerant Lines in Rice  

Lee, In-Sok (Korea Atomic Energy Research Institute)
Kim, Dong-Sub (Korea Atomic Energy Research Institute)
Hyun, Do-Yoon (Korea Atomic Energy Research Institute)
Lim, Yong-Pyo (Chungnam National Univ., Department of Horticulture)
Lee, Young-Il (Korea Atomic Energy Research Institute)
Publication Information
Journal of Plant Biotechnology / v.29, no.4, 2002 , pp. 247-252 More about this Journal
Abstract
The combination of radiation technique with an in vitro culture system was initiated to develop salt tolerant rice. We established an in vitro culture system to select tolerant lines against salt stress. NaCl tolerant cell lines were selected from the callus irradiated with gamma ray on N$_{6}$ medium with 1.5% NaCl and 2 mg/L 2,4-D. Regenerants (M$_1$) were obtained from the tolerant callus which was cultured for 30 days auxin-free medium. The M$_2$seeds were harvested from M$_1$plants on an individual plant basis. Thirty seedlings from each 450 M$_2$lines were transplanted in a field and total 5,000 M$_3$lines were harvested with an average 90 percent of fertile grain. M$_3$lines were utilized for selection of salt tolerance. Salinity-tolerant lines (225) were selected among 5,000 M$_3$lines. Of the 225 lines tested, the morphological traits of two lines (120-10 and -11) were far superior to control (Donagjinbyeo) in agromomic traits such as plant height, root length and no. of roots. Control and tolerant lines were analyzed by RAPD markers. Three polymorphic bands were presented in only tolerant lines, demonstrating a genetic difference between control and the tolerant lines. Such tolerant lines could be used as genetic resources to improve salt tolerance.e.
Keywords
Callus; irradiation; rice; salt tolerance;
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