• Korean Journal of Food Science and Technology
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• v.33 no.5
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• pp.582-588
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• 2001

To control the growth of Salmonella on raw chicken supplied in food service, they were isolated and analysed for their physiological characteristics. Total viable microbe counts under the skin of the chicken amounted to 10% of numbers on their skin. Fifty one of Salmonella were isolated and identified from 75 chicken carcass samples. About 70% of the isolates showed resistance to more than four antibiotics, which indicated very high resistance among the strains ever reported in Korea. Lactic acid and trisodium phosphate(TSP) as sanitizing agent were applied to the Salmonella spp. isolates by agar diffusion method. The resistance by isolates to those sanitizing agents was compared with type strains of S. typhimurium ATCC 14028, S. typhimurium ATCC 12023, S. heidelberg and S. enteritidis. The isolates showed similar or less resistance to the type strains as for the lactic acid. However, as for trisodium phosphate known recently as a good sanitizer, 50% of the isolates showed more resistant compared with the type strains. Also, the treatment time and regeneration medium affected significantly the resistance of the isolates, which indicates that more careful test might be needed for TSP's practical application to raw chicken. Therefore, From the results, it is suggested that antibiotic treatment during breeding broiler and contamination after slaughtering should be minimized along with careful use of sanitizing agent in order to ensure more safe raw chicken supply.

• Microbiology and Biotechnology Letters
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• v.30 no.3
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• pp.216-222
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• 2002

To Produce the modified Cheongiu that has high ethanol content, an ethanol-tolerant strain Saccharo-myces cerevislae SE2l1 was screened from Saccharomyces cerevisiae Kyokai No. 10 strain. The isolate showed faster growth than in the medium containing 10% ethanol compared with original strain. The isolate produced a higher concentration of ethanol and showed higher resistance to ethanol, high osmolarity and heat than the original strain. The analyses of yeast membrane components indicated that there were no significant changes in composition of sterols and phospholipids between the isolated and the original strain. However, during the fermentation, the iso-lated strain could change the fatty acid composition in the membrane more rapidly in the direction of decreasing membrane unsaturation and accumulate more trehalose in the cell than the original strain. These data suggest that the ability to change its membrane fatty acid composition and to accumulate trehalose may make the isolated strain easily adapt to changes in external condition.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.7
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• pp.676-681
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• 2010

Antibiotic resistant bacteria were isolated from livestock wastes and the resistance patterns were investigated using various antibiotic agents. Also, a gamma ray was tested regarding the aspects of the effect on resistance pattern and the efficiency of disinfection. Among the isolates, Esherichia coli and Brevibacterium sp. showed the most serious resistance patterns. Esherichia coli had resistance against 9 agents whereas Brevibacterium sp. against 7 agents. It can be suggested from these results that the abuse of antibiotic agents will cause a serious mutation problem even to Esherichia coli which is ubiquitous in the ecosystem. Esherichia coli could be easily controlled but Brevibacterium sp. had a moderate resistance to the gamma ray under low doses. In the case of Brevibacterium sp, more than 2.0 kGy of a radiation dose will be required in order to achieve an enhanced efficiency of disinfection.

• Tuberculosis and Respiratory Diseases
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• v.54 no.3
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• pp.295-303
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• 2003

Backgroung : The incidence of penicillin-resistant streptococcus pneumoniae(PRSP) accounts for almost 70% of all pneumococcal pneumonia cases in Korea. It is still unclear as to whether the efficacy of penicillin or equally active beta-lactam agents is compromised in PRSP pneumonia. This study investigated the prevalence of PRSP in community-acquired pneumonia and its clinical course. Methods : A total of 42 patients with community-acquired pneumococcal pneumonia were evaluated from July 1999 to May 2001. The cultured strains of Streptococcus pneumoniae were divided into susceptible, intermediately resistant, and resistant strains by an E-test, and the effect of the clinical course was investigated. Results : From a total of 42 patients, 22 (52.4%) patients had an intermediate resistance (MIC $0.1-1{\mu}g/m{\ell}$) and six (14.3%) showed a high resistance ($MIC{\geq}2.0{\mu}g/m{\ell}$) with current penicillin susceptibility categories. However, according to the classification of the DRSPTWG (Drug Resistant Streptococcus pneumoniae Therapeutic Working Group), there were 11 cases (26.2%) of intermediate resistance and no case of high resistance. Under empirical antimicrobial treatment, there was no difference in the clinical outcome between the penicillin susceptible and resistant group. Conclusion : The clinical outcome of PRSP pneumonia with empirical therapy was acceptable. These results suggest that the current MIC breakpoint for penicillin resistance in Streptococcus pneumoniae has been set at a very low level and penicillin resistance according to the NCCLS classification does not significantly influence the outcome of the empirical treatment for pneumococcal pneumonia.

• Tuberculosis and Respiratory Diseases
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• v.46 no.5
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• pp.618-627
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• 1999

Background : Rifampicin (RFP) is a key component of the antituberculous short-course chemotherapy. Usually the RFP resistant M.tuberculosis is also resistant to isoniazid (INH), so the RFP resistance is the marker of multi-drug resistant (MDR) tuberculosis. But unusual cases of mono-RFP-resistant tuberculosis have been recently reported with increasing frequency, especially associated with HIV infection in western countries. Therefore, we conducted a retrospective study to investigate the frequency, causes, and the clinical characteristics of mono-RFP-resistant tuberculosis in Korea. Methods : Of the bacteriologically confirmed and susceptibility-proven 699 pulmonary tuberculosis patients (921 isolates) who visited Asan Medical Center from January 1990 to August 1997, eighteen patients with INH-susceptible and RFP-resistant tuberculosis were evaluated. Previous history of tuberculosis, antituberculous drug compliances, associated systemic illness, drug susceptibility patterns, and clinical outcomes were analysed. And rpoB gene sequencing was done in 6 clinical isolates of M. tuberculosis. Results : The mean age of 18 patients was $43{\pm}14$ years, and the sex ratio is 12:6 (M : F). Sixteen (89%) patients had previous history of tuberculosis. None had diagnosed gastrointestinal disorders, and 2 HIV tests that were performed came out negative. Susceptibility tests were done repeatedly in eleven patients, and six (55%) were mono-RFP resistant repeatedly while five (45%) evolved to MDR tuberculosis. Eight (44%) patients were cured, six (33%) failed, three (17%) were lost to follow-up, and the other one is now on treatment. rpoB gene sequencing showed 5 mutations, codon 531 TCG to TIG mutation in 4 isolates and 526 CAC to TAC in 1 isolate. Conclusion : The clinical characteristics of mono-RFP resistant tuberculosis were similar to that of MDR tuberculosis in Korea where the HIV infection rate is lower than western countries. But some patients with mono-RFP-resistant tuberculous could be cured by primary drug regimens including RFP, suggesting that mono-RFP-resistant tuberculous is a different entity from MDR tuberculosis.

• Pediatric Infection and Vaccine
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• v.18 no.1
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• pp.23-30
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• 2011

Purpose : Acinetobacter baumannii is an aerobic, gram negative coccobacillus. Due to its pathogenicity and ability to accumulate diverse mechanisms of resistance, the importance of this organism is increasing. Many reports have targeted adults, and studies of pediatric patients are limited. This study aims to investigate the current status of A. baumannii infection in children. Methods : From January 2001 to December 2008, 505 patients hospitalized with A. baumannii infection were enrolled. Admission records for underlying disease, duration of hospitalization, previous antibiotic use, location of admission, presence of ventilator care, and resistance to antibiotics were retrospectively reviewed and analyzed. Results : Hemato-oncological disease and neurological disease were 30.6% and 24.3% of all cases; therefore, these were the most common underlying diseases of patients with A. baumannii infection. Prevalence of A. baumannii infection was 78.1% in patients with previous antibiotic use, which was higher than that of the group not using previous antibiotic. And prevalence of multi-drug resistant and pan-drug resistant A. baumannii infection was 76.4% and 38.3% in patients with ICU care, 76.8% and 38.9% with ventilator care, and these were higher than the others. Rate of resistance to all groups of antibiotics showed a gradual increase to over 50% in 2008. Multi-drug resistant A. baumannii was 63.5% and pan-drug resistant A. baumannii was 48.2% of all cases. Conclusion : Prevalence of A. baumannii infection and resistance to antibacterial agents of A. baumannii is increasing. Adequate use of antibiotics and infection control should be emphasized in pediatric patients.

• Proceedings of the Korean Information Science Society Conference
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• 2003.04a
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• pp.157-159
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• 2003

신뢰할 만한 이동 에이전트 시스템을 구축하기 위해서는 이동 에이전트의 결함 내성 기능이 중요하다. 지금까지 여러 결함 내성 기법이 제안되었는데. 그 중의 하나가 에이전트 복제 기법이다. 에이전트 복제는 검사점 기법에 대해 훨씬 나은 신뢰성을 제공하는 반면, 복제 에이전트의 이동과 동의 과정에 수반되는 부하가 시스템의 성능을 저하시킨다. 그러므로 본 논문에서는 비동기 에이전트 복제 기법을 제안한다. 이 기법에서는 에이전트를 비동기적으로 전송하며 동의 과정을 위해 뒤늦게 전송되는 복제 에이전트를 기다리는 것이 아니라 고정된 동의 전용 에이전트를 사용하여 동의 과정의 비용을 감소시킨다. 제안된 기법은 Aglet 시스템 상에 구현되었으면. 실험 결과 제안된 기법을 사용할 경우 35%-69%의 성능 향상을 얻을 수 있었다.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2002.05a
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• pp.371-372
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• 2002

• Korean Journal of Environmental Agriculture
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• v.16 no.1
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• pp.55-60
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• 1997

Heavy metal-tolerant microorganisms, such as Pseudomonas putida, P. aeruginosa, P. chlororaphis and P. stutzeri which possessed the ability to accumulate cadmium, lead, zinc and copper, respectively, were isolated from industrial wastewaters and mine wastewaters polluted with various heavy metals. The distribution of heavy metal in the cell components, and amino acid compositions, was investigated. The distribution of heavy metal in the cell fractions of each heavy metal-tolerant microorganism grown for 20 hours in the basal medium containing 100mg/l of each heavy metal was investigated. In the case of cadmium-tolerant P. putida, lead-tolerant P. aeruginosa and copper-tolerant P. stutzeri, approximately $50{\sim}60%,\;30{\sim}40%$ and $10{\sim}17%$ of each heavy metal absorbed were distributed to cell wall, cell membrane and cytoplasm fractions, respectively. In the case of zinc-tolerant P. chlororaphis, approximately 32%, 55% and 13% of zinc were distributed to cell wall, cell membrane and cytoplasm fractions, respectively. These results indicated that the cell wall was a major adsorbing fraction of cadmium, lead and copper, and the cell membrane was that of zinc. Total amino acid content per gram of the cell grown in the culture media with heavy metal was higher than that of the cell grown in the culture media without heavy metal, and the content of acidic amino acids, such as aspartic acid(Asp.+Asn.) and glutamic acid(Glu.+Gln.) was higher than that of basic amino acids, such as histidine, lysine and arginine.

• Proceedings of the Korean Society of Food Hygiene and Safety Conference
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• 2002.05a
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• pp.166-169
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• 2002

유전자재조합(GM)식품에 대해 듣거나 읽어서 알고 있는 사람이 67%정도 되었고,GM식품을 자발적으로 먹겠다는 사람은 18%를 약간 상회하였고 기다려보고 결정하겠다는 사람들이 약 50%정도였다. 농약 등의 화학물질 오염이나 취급부주의 및 기업인의 윤리의식에 대한 불신에 비해 GM식품에 대한 우려는 매우 적게 나타났으나 GM방법에 의해 개발한 제초제 내성 콩을 먹겠다는 사람은 적은 데 비해 같은 방법으로 개발한 비타민이 많은 콩을 먹겠다는 사람은 많은 것으로 보아 소비자들은 GMO이기 때문에 제초제 내성 콩을 거부하는 것이 아니라, 제초제 내성 콩에 대한 내용의 이해부족으로 인한 거부감을, 그리고 비타민 콩은 비타민이 인체에 중요한 영양소라는 것 때문에 호감을 가지는 것으로 해석되었다. 전체적으로 소비자들이 GMO에 관한 정보를 많이 접하기는 하지만 보통 식품에도 유전자가 있다는 사실을 아는 소비자들이 조사 대상자의 40%정도인 것으로 보아 노출된 만큼 내용을 잘 알지 못하는 것으로 나타났다. GM식품에 대하여는 90%이상이 표시하기를 바라고 있는 것과 같이 2001년 7월부터 가공식품에 GM원료 함유여부 표시제도가 시행되고 있는 데, 이 제도의 시행 사실을 알고 있는 소비자는 22%에 지나지 않았다. 효과적인 표시제도의 시행을 위해서는 일반시민이 제도의 시행에 대해 잘 숙지하고 있어야 할 것이다.