• Title/Summary/Keyword: 내생균

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Species Diversity and Antifungal Activity of Endophytic Fungi Isolated from Angelica gigas Nakai (참당귀에서 분리된 내생균의 다양성과 병원균 억제 효과)

  • Park, Hyeok;Jung, Chung Ryul;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.49 no.4
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    • pp.497-505
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    • 2021
  • Endophytic fungal strains were isolated from leaves, stems, and roots of Angelica gigas. The fungal strains were identified based on their morphological characteristics and molecular analysis of the internal transcribed spacer (ITS). A total 35 species of endophytic fungi were identified. The diversity between the endophytic fungal communities differed depending on the tissues of A. gigas. The isolated endophytic fungi were screened for antifungal activity against a pathogenic fungus, Phoma sp. Y11, using a dual culture method. Fourteen species of endophytic fungi showed the standout inhibition effect against the Y11 strain. The results suggest that the endophytic fungi isolated from A. gigas could be used as a biological control agent against leaf spot disease of A. gigas.

Geographical Patterns and Biodiversity of Endophytic Fungi Isolated from Scirpus L. s.l. in Korea (고랭이류에서 분리한 내생균의 생물다양성 및 지리적 양상)

  • Eo, Ju-Kyeong;Park, Eunsu
    • The Korean Journal of Mycology
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    • v.47 no.1
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    • pp.43-50
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    • 2019
  • We investigated the biodiversity of endophytic fungi of Bolboschoenus planiculmis and Schoenoplectus triqueter in Korea. Twenty five of these host plants were collected from six sampling sites. Internal transcribed spacer analysis identified a total of eighty five isolates from the collected plants. The isolates comprised nineteen taxa; most of the isolates (92%) belonged to class Dothideomycetes, followed by Sordariomyetes (4.8%), Agaricomycetes (1.6%), and Eurotiomycetes (0.8%). The most prevalent fungus was Cladosporium perangustum in B. planiculmis and Macrospora scirpicola in S. triqueter. There are very few studies of endophytic fungi in halophytes and these have reported biodiversity patterns different from those of previous studies in Korea. Our results should provide useful information for future research in Korea.

Molecular Identification of Endophytic Fungi Isolated from Needle Leaves of Pinus thunbergii (곰솔(Pinus thunbergii)의 침엽에서 분리한 내생균의 분자적 동정)

  • Kim, Chang-Kyun;Eo, Ju-Kyeong;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.40 no.4
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    • pp.183-186
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    • 2012
  • Endophytic fungi were isolated from needle leaves of Pinus thunbergii distributed in Boryeong of Korea. Total 7 species of fungi were identified using partial sequences of ITS and LSU regions of nuclear DNA Anthostomella sepelibilis, Beltrania sp., Cladosporium sp., Coniochaeta velutina, Entonaema pallida, Lophodermium sp. and Nigrospora sphaerica sphaerica. The endophytic fungi, Beltrania sp. was the most dominant species isolated from P. thunbergii in this study. Results indicate that the distribution of endophytic fungi in the leaves of P. thunbergii could be influenced by environments of the fungal habitat.

Diversity of Foliar Endophytic Fungi Isolated from Lindera obtusiloba in Korea (생강나무(Lindera obtusiloba)의 잎에서 분리한 내생균의 다양성)

  • Kim, Chang-Kyun;Eo, Ju-Kyeong;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.40 no.3
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    • pp.136-140
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    • 2012
  • Leaves of Lindera obtusiloba were collected from four sites in Gangwon Province, Korea. Endophytic fungi were isolated from the leaves and identified using ITS sequences of rDNA. Total twelve species belonging of endophytic fungi were identified; Alternaria alternata, Annulohypoxylon annulatum, Creosphaeria sassafras, Diaporthe eres, Discosia sp., Epicoccum nigrum, Glomerella acutata, Glomerella cingulata, Paraconiothyrium brasiliense, Pestalotiopsis neglecta, Phomopsis amygdali, Xylaria sp. The endophytic fungus, Phomopsis amygdali, was the most dominant species isolated from L. obtusiloba in this study and the fungal diversities varied in the different sites.

Two Unrecorded Enophytic Fungi Isolated from Root of Ligusticum chuanxiong in Korea: Pithomyces chartarum and Plectosphaerella niemeijerarum (토천궁의 뿌리에서 분리된 2종의 국내 미기록 내생균: Pithomyces chartarum and Plectosphaerella niemeijerarum)

  • Park, Hyeok;Jung, Chung Ryul;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.47 no.4
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    • pp.329-335
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    • 2019
  • Endophytic fungal strains were isolated from the sterilized roots of a medicinal plant, Ligusticum chuanxiong and identified based on morphological characteristics and molecular analysis of internal transcribed spacer, large subunit rDNA, and beta-tubulin DNA regions. Our results confirmed the presence of Pithomyces chartarum and Plectosphaerella niemeijerarum in the fungal strains. To the best of our knowledge, the fungal strains have not been reported in Korea. In this report, we describe morphological characteristics and phylogenetic trees of these endophytic fungal strains.

Two Unrecorded Endophytic Fungi Isolated from Funaria hygrometrica in Korea (표주박이끼(Funaria hygrometrica)에서 분리된 2종의 국내 미기록 내생균)

  • Choi, Hyun-Sook;Park, Hyeok;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.47 no.4
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    • pp.313-318
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    • 2019
  • In the present study, we isolated endophytic fungal strains from the rhizoids of the moss Funaria hygrometrica. The isolated strains were identified based on morphological characteristics and analysis of the internal transcribed spacer (ITS) and large subunit (LSU) rDNA sequence regions. Consequently, we confirmed the presence of two endophytic fungal species, Curvularia protuberata and Didymella anserina, which have not been reported in Korea previously. Here, we describe the morphological characteristics and molecular analysis results of these fungal species.

New Records of Endophytic Fungi Isolated from Leaves of Abies koreana and Taxus cuspidata in Korea (구상나무와 주목의 침엽에서 분리된 국내 미기록 내생균)

  • Park, Hyeok;Kim, Dong-Yeo;Kim, Sae-Rom;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.46 no.3
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    • pp.241-248
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    • 2018
  • We isolated endophytic fungi from the coniferous leaves of Abies koreana and Taxus cuspidata inhabiting Mt. Hallasan, Korea. The isolated fungal strains were identified based on a phylogenetic analysis using nucleotide sequences of the internal transcribed spacer region, large subunit region of ribosomal DNA, and translation elongation factor region. Results confirmed four prevously unreported endophytic fungi in Korea: Lachnellula hyalina, Ochrocladosporium elatum, Phacidium lacerum, and Phyllosticta cussoniae. In this report, we describe the morphological characteristics of these fungi and the results of the phylogenetic analysis.

Unrecorded Endophytic Fungi Isolated from Mnium heterophyllum and Hypnum plumaeforme in Korea: Biscogniauxia petrensis and Cercophora thailandica (꼬마초롱이끼(Mnium heterophyllum)와 가는털깃털이끼(Hypnum plumaeforme)에서 분리한 국내 미기록 내생균: Biscogniauxia petrensis, Cercophora thailandica)

  • Choi, Hyun-sook;Park, Hyeok;Eo, Ju-Kyeong;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.48 no.1
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    • pp.55-61
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    • 2020
  • In this study, we isolated endophytic fungal strains from the rhizoid and the leaf of Mnium heterophyllum and Hypnum plumaeforme, respectively. The isolated strains were identified based on morphological characters and molecular analysis of the internal transcribed spacer, large subunit rDNA, β-tubulin, and RNA polymerase II subunit regions. From the results, we confirmed two endophytic fungal species, Cercophora thailandica, and Biscogniauxia petrensis, which to the best of our knowledge, have not yet been reported in Korea. We further describe the morphological characteristics and the results of the molecular analyses of these isolated fungal strains.

Diversity of Endophytic Fungi Isolated from the Rootlet of Pinus densiflora Colonized by Tricholoma matsutake (송이버섯과 공생하는 소나무 세근으로부터 분리된 내생균의 다양성)

  • You, Young-Hyun;Yoon, Hyeok-Jun;Woo, Ju-Ri;Rim, Soon-Ok;Lee, Jin-Hyung;Kong, Won-Sik;Kim, Jong-Guk
    • The Korean Journal of Mycology
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    • v.39 no.3
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    • pp.223-226
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    • 2011
  • Endophytic fungi were isolated from the Pinus densiflora rootlet colonized by ectomycorrhizal fungus Tricholoma matsutake. Eighteen species of endophytic fungi were identified by analyzing rDNA-ITS sequence. As the result of the rDNA-ITS analysis, ascomycota of 15 species and Mucoromycotina of 3 species were isolated. Of all the endophytic fungi isolated, Penicillium sp. was confirmed as the highest frequency.

Characterization of Six Novel Endophytic Fungi Isolated from Leaves of Plants Inhabiting Jeju Island (제주도에 서식하는 식물 잎에서 분리된 6종의 국내 미기록 내생균 보고)

  • Park, Hyeok;Choi, Young-Joon;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.46 no.4
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    • pp.405-414
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    • 2018
  • Endophytic fungi were isolated from the leaves of diverse plants inhabiting Jeju Island, Korea. The fungal isolates were identified through phylogenetic analyses incorporating nucleotide sequences derived from the internal transcribed spacer region, large subunit region of ribosomal DNA, and beta-tubulin gene. Our results identified six endophytic fungi previously unknown in Korea namely, Diaporthe goulteri, Diaporthe vaccini, Rhizosphaera pini, Valsa friesii, Xylaria primorskensis, and Zalerion arboricola were identified. Here, we present their cultural and morphological characteristics and phylogenetic relationship.