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Two Unrecorded Endophytic Fungi Isolated from Funaria hygrometrica in Korea

표주박이끼(Funaria hygrometrica)에서 분리된 2종의 국내 미기록 내생균

  • Choi, Hyun-Sook (Department of Biology Education, Korea National University of Education) ;
  • Park, Hyeok (Department of Biology Education, Korea National University of Education) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 최현숙 (한국교원대학교 생물교육과) ;
  • 박혁 (한국교원대학교 생물교육과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2019.10.28
  • Accepted : 2019.12.12
  • Published : 2019.12.31

Abstract

In the present study, we isolated endophytic fungal strains from the rhizoids of the moss Funaria hygrometrica. The isolated strains were identified based on morphological characteristics and analysis of the internal transcribed spacer (ITS) and large subunit (LSU) rDNA sequence regions. Consequently, we confirmed the presence of two endophytic fungal species, Curvularia protuberata and Didymella anserina, which have not been reported in Korea previously. Here, we describe the morphological characteristics and molecular analysis results of these fungal species.

표주박이끼의 헛뿌리(rhizoid)에서 내생균을 분리하였다. 분리된 균주는 형태적 특성 및 internal transcribed spacer, large subunit rDNA 영역의 분자적 분석을 토대로 동정하였다. 연구 과정에서 2종의 국내 미기록종 내생균 균주를 확인하였고, 확인된 종은 Curvularia protuberata, Didymella anserina이다. 미기록종 내생균 균주의 형태적 특성 및 계통 분석 결과에 대해 기술하였다.

Keywords

References

  1. Petrini L. Xylariaceous fungi as endophytes. Sydowia 1985;38:216-34.
  2. Clay K. Fungal endophytes of grasses: a defensive mutualism between plants and fungi. Ecology 1988;69:10-6. https://doi.org/10.2307/1943155
  3. Partida-Martinez LPP, Heil M. The microbe-free plant: fact or artifact? Front Plant Sci 2011;2:100. https://doi.org/10.3389/fpls.2011.00100
  4. Strobel GA, Hess WM, Ford E, Sidhu R, Yang X. Taxol from fungal endophytes and the issue of biodiversity. J Ind Microbiol 1996;17:417-23. https://doi.org/10.1007/bf01574772
  5. Findlay JA, Buthelezi S, Lavoie R, Pena Rodriguez L, Miller JD. Bioactive isocoumarins and related metabolites from conifer endophytes. J Nat Prod 1995;58:1759-66. https://doi.org/10.1021/np50125a021
  6. Santos-Fo F, Fill TP, Nakamura J, Monteiro MR, Rodrigues-Fo E. Endophytic fungi as a source of biofuel precursors. J Microbiol Biotechnol 2011;21:728-33. https://doi.org/10.4014/jmb.1010.10052
  7. Park JH, Choi GJ, Lee HB, Kim KM, Jung HS, Lee SW, Jang KS, Cho KY, Kim JC. Griseofulvin from Xylaria sp. strain F0010, an endophytic fungus of Abies holophylla and its antifungal activity against plant pathogenic fungi. J Microbiol Biotechnol 2005;15:112-7.
  8. Sherameti I, Tripathi S, Varma A, Oelmuller R. The root-colonizing endophyte Pirifomospora indica confers drought tolerance in Arabidopsis by stimulating the expression of drought stress-related genes in leaves. Mol Plant Microbe Interact 2008;21:799-807. https://doi.org/10.1094/MPMI-21-6-0799
  9. Schulz B, Boyle C, Draeger S, Rommert A-K, Krohn K. Endophytic fungi: a source of novel biologically active secondary metabolites. Mycol Res 2002;106:996-1004. https://doi.org/10.1017/S0953756202006342
  10. Zhang T, Zhang YQ, Liu HY, Wei YZ, Li HL, Su J, Zhao LX, Yu LY. Diversity and cold adaptation of culturable endophytic fungi from bryophytes in the Fildes Region, King George Island, maritime Antarctica. FEMS Microbiol Lett 2013;341:52-61. https://doi.org/10.1111/1574-6968.12090
  11. Kim JH, Kim DY, Park H, Cho JH, Eom AH. Neocosmospora rubicola , an unrecorded endophytic fungus Isolated from roots of Glycyrrhiza uralensis in Korea. Kor J Mycol 2017;45:63-7. https://doi.org/10.4489/KJM.20170007
  12. Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes-application to the identification of mycorrhizae and rusts. Mol Ecol 1993;2:113-8. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  13. Moncalvo JM, Lutzoni FM, Rehner SA, Johnson J, Vilgalys R. Phylogenetic relationships of agaric fungi based on nuclear large subunit ribosomal DNA sequences. Syst Biol 2000;49:278-305. https://doi.org/10.1080/10635159950173852
  14. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016;33:1870-4. https://doi.org/10.1093/molbev/msw054
  15. Nelson R, Hodges CS. A new species of Curvularia with a protuberant conidial hilum. Mycologia 1965;57:822-5. https://doi.org/10.2307/3757020
  16. Isenor M, Kaminskyj SG, Rodriguez RJ, Redman RS, Gough KM. Characterization of mannitol in Curvularia protuberata hyphae by FTIR and Raman spectromicroscopy. Analyst 2010;135:3249-54. https://doi.org/10.1039/c0an00534g
  17. Morsy MR, Oswald J, He J, Tang Y, Roossinck MJ. Teasing apart a three-way symbiosis: transcriptome analyses of Curvularia protuberata in response to viral infection and heat stress. Biochem Biophys Res Commun 2010;401:225-30. https://doi.org/10.1016/j.bbrc.2010.09.034
  18. Chen Q, Jiang J, Zhang G, Cai L, Crous PW. Resolving the Phoma enigma. Stud Mycol 2015;82:137-217. https://doi.org/10.1016/j.simyco.2015.10.003
  19. De Gruyter J, Noordeloos M. Contributions towards a monograph of Phoma (Coelomycetes)-I. 1. Section Phoma: Taxa with very small conidia in vitro. Persoonia 1992;15:71-92.