• Korean Journal of Plant Taxonomy
• /
• v.40 no.3
• /
• pp.183-185
• /
• 2010

We report a unrecorded species, Da-do-sae-u-nan-cho (Calanthe ${\times}$ kibanakirishima F. Maek). It was discovered on Heuksan Island in Jeollanam Province. The species differs from C. aristulifera in the corolla color and the short, wide spur. It is also easily distinguished from the other species of Calanthe by the simple trichomes on the abaxial surface of its leaves and spur.

• Korean Journal of Plant Taxonomy
• /
• v.42 no.3
• /
• pp.215-221
• /
• 2012

Five Korean Calanthe species, C. discolor, C. bicolor, C. sieboldii, C. reflexa, and C. aristulifera, were studied using amplified fragment length polymorphism (AFLP) to assess their taxonomic and genetic relationships. Sixteen accessions belonging to five native Calanthe spp. and mutants with yellow tepal and white lip (YW mutants) were studied. We identified 50 putative markers using AFLP analysis. The results of AMOVA showed that genetic variance was higher between species than within species. Genetic dissimilarity when compared with the rest of the species was the lowest for individuals of the YW mutants and the highest for individuals of C. reflexa. The mutants clustered outside the major group. Calanthe bicolor clustered with C. discolor, suggesting that its genetic composition is closer to that of C. discolor. Though it is suggested to have originated as a result of natural hybridization between C. sieboldii and C. discolor, introgression is likely to have occurred in the direction of C. discolor based on the data of molecular marker, clustering and genetic dissimilarity. Calanthe reflexa and C. aristulifera were genetically the most diverse of the species studied. In conclusion, the results showed that there is genetic diversity in Korean Calanthe species, that C. bicolor introgressed in the direction of C. discolor and that the YW mutants are genetically closer to C. sieboldii.

• Korean Journal of Plant Taxonomy
• /
• v.43 no.3
• /
• pp.223-226
• /
• 2013

An unrecorded Orchid species, Habenaria dentata (Sw.) Schltr. (Orchidaceae), was recognized in Gahoe-myeon and Ssangchaek-myeon, Hapcheon-gun, Gyeongsangnam-do, South Korea. H. dentata is closely related to H. radiata (Thunb.) Spreng., it can distinguished by sepal white and larger than petals, and serrated margins of the labellum. A new Korean name, 'Keun-hae-o-ra-bi-nan-cho', was given based on its taller stem and more flowers (at least with three) in comparison to H. radiata (Thunb.) Spreng. We provide here its redescription, illustrations, photographs, and a key to its closely related taxa.

• The Korean Journal of Mycology
• /
• v.43 no.2
• /
• pp.129-132
• /
• 2015

In this study, orchid mycorrhizal fungi (OMF) were isolated from four terrestrial orchids on Mt. Hambeak, Platnathera chlorantha, Platnathera mandarinorum, Cephalanthera falcate, and Cephalanthera longibracteata. OMF were identified using morphological and sequences analysis of fungal internal transcribed spacer (ITS) regions by specific primer of basidiomycetous orchid mycorrhizas; ITS1-OF and ITS4-OF. Four species of orchid mycorrhizal fungi were identified as Ceratobasidium sp, Epulorhiza anaticula, Tulasnella calospora and Tulasnella sp.

• Korean Journal of Plant Taxonomy
• /
• v.43 no.3
• /
• pp.161-164
• /
• 2013

Neottia japonica (Blume) Szlach. (Orchidaceae), previously known to be native in southern part of Japan and Taiwan, was found in Seogwipo-si, Jeju-do, Korea. This species is distinguished from the related taxa of Neottia pinetorum (Lindle.) Szlach and N. nipponica (Makino) Szlach. by smaller plant, lip with T-shaped lamella and auricles embracing column, and flowering in the spring. The local name was given as "A-gi-ssangnip-nan-cho" based on the cuter and smaller plant than N. pinetorum. The description and illustrations of the species on morphological characters, voucher specimens, and photograph at the habitat are provided.

• Korean Journal of Plant Taxonomy
• /
• v.39 no.4
• /
• pp.292-295
• /
• 2009

Here, we report a previously unrecorded species, Sin-an-sae-u-nan-cho (Calanthe aristulifera Rchb. f.). It was discovered on Heuksan Island in Jeollanam-do. Its natural habitat was a subtropical forest. The species can easily be characterized by the simple trichomes on the back of its leaves and spur. This species is important because new horticultural races of Calanthe can be developed by cross-breeding with other species of Calanthe.

• Korean Journal of Plant Taxonomy
• /
• v.37 no.4
• /
• pp.395-400
• /
• 2007

Habenaria iyoensis Ohwi (Orchidaceae), previously known only from southern Japan and Taiwan, was found in the lowland of Jeju-do. This species is distinguished from the related taxa of Korean Habenaria by 3-nerved sepals, short-cylindrical spurs, and rosette leaves. The local name Ae-gi-bang-ul-nan-cho implies a smaller plant than H. flagellifera. The morphological characters and illustrations of the species are provided with photographs at the habitat.

• Journal of Life Science
• /
• v.17 no.3 s.83
• /
• pp.312-317
• /
• 2007

This study was conducted to research the morphological characteristics and analyze the genetic diversity by using RAPD in Calanthe species native to Korea. Nine samples were selected by flower color and 19 morphological characteristics. In the length and width of leaf, dorsal sepal, the lateral sepal, the petal, the central lip, and the lateral lip, C. discolor was the shortest and narrowest, respectively, but C. sieboldii was the longest and the widest, respectively. The flower stalk length was the shortest in C. discolor, and the longest in C. sieboldii. Three variants were the intermediate between C. discolor and C. sieboldii in the above morphological characteristics, but spur length was the longest in C. discolor, the shortest in C. sieboldii, and intermediate in the variants. The ovary length in C. discolor was shortest and C. sieboldii and variants were similar with each other. The flower color of C. discolor were brownish red, the value of CIE Lab was between 40 and 50. The flower color of C. sieboldii was yellowish, the value of CIE Lab was between 110 and 130. And variants had various colors between 50 to 70 in the value of CIE Lab. By analyzing multiple band patterns of PCR products, 154 bands were selected as polymorphic RAPD markers. The analysis of genetic similarity of Calanther species using RAPD showed that C. discolor and C. sieboldii are more distant from each other than variants, and these results demonstrated that genetic position of variants located between C. discolor and C. sieboldii.

• Korean Journal of Plant Taxonomy
• /
• v.36 no.2
• /
• pp.109-127
• /
• 2006

A taxonomic study of Habenaria in Korea was carried out based on the extensive examinations of floral morphologies and distribution. As the results, we classified the genus in Korea into the following 5 species; H. linearifolia Maxim., H. cruciformis Ohwi, H. radiata Splengl., H. chejuensis Y.N. Lee & K.S. Lee and H. flagellifera Makino. H. cruciformis is recognized as a distinct species distinguished from H. linearifolia, H. sagittifera Rchb. f. and H. schindleri Schltr., and we clarified its morphological differences from the species. H. linearifolia f. integrilova Ohwi which has been seperated by shape of lateral lobe of lip was merged into f. linearifolia oin this study. Furthermore we investigated the distribution of Habenaria in Korea based on the herbarium specimens and field surveys. The result shows that H. radiata is rare in the middle part of the Korean Peninsula and H. chejuensis and H. flagellifera are restricted in Jeju Island, so the conservation on the habitats of these species is required.

• Korean Journal of Plant Resources
• /
• v.21 no.4
• /
• pp.341-345
• /
• 2008

Two orchid species of Calanthe discolor Lindl. and C. discolor for. sieboldii (Decne.) Ohwi, which have different form flower color and size. They were crossed in mid April by artificial pollination, and the F1 hybrid seeds were collected mid October. Germination of seeds was investigated on pre-treatment of seeds and under the various environmental conditions. Germination was promoted by moisture absorption and ultrasonic treatment of seeds. Dark culture of F1 hybrid seeds enhanced germination and protocorm formation, and development into seedlings compared with light culture. Although, plant growth regulators such as NAA and BA had a slightly promotive effect on seed germination and protocorm growth, regenerated seeding were showed abnormal growth patterns. Regenerated F1 hybrid plantlets were successfully transferred to pot.