• Title/Summary/Keyword: 기초 분리

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A Study on the Comparison of Liang Shu-Ming and Lee Don-Hwa's Perceptions of Modernization and the Philosophical Grounds of their Perceptions : Focusing on Works during the New Cultural Movement Period in Korea and China (양수명과 이돈화의 근대화에 대한 견해 및그 철학적 근거에 대한 비교 연구 - 한·중 신문화운동 시기의 저작을 중심으로 -)

  • Hwang, Jong Won
    • The Journal of Korean Philosophical History
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    • no.36
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    • pp.319-352
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    • 2013
  • This paper aims to compare Liang Shu-Ming and Lee Don-Hwa's perceptions of modernization and the philosophical grounds of their perceptions during the New Cultural Movement period in Korea and China. Although Liang and Lee had different levels of enthusiasm towards New Cultural Movement, they shared many commonalities in their attitudes to Western modernization and their philosophical justifications for such attitudes. Liang Shu-Ming not only held positive views of Western modernization, but also examined the spiritual dynamics contributing to the development in science and democracy in Western countries. By analyzing the three fundamental questions of life, three kinds of human attitudes toward life, three types of culture derived from human attitudes, and the recapitulation theory, Liang systematically reviewed the spiritual dimension of Western modernization. Liang defined the spirit of Western modernization as a calculative rationality based on egoism and the separation of Self and Other. Such a statement demonstrates Liang's keen insights towards Western modernization. Meanwhile, although Lee Don-Hwa basically held positive views of Western modernization, from the beginning, he pointed out the problems with Western modernization and attempted to overcome these problems by employing the "human is heaven" doctrine of Cheondogyo. While Lee embraced modern science and the theory of evolution, he also insisted on the legitimacy of mysticism and the compatibility of the creation theory of Cheondogyo and the theory of evolution. Although Lee employed the concepts in Western philosophy of life, he also emphasized the interdependence, reciprocal relationship, and connectedness between Self and Other from the perspective of Cheodogyo doctrine. From the fact that Lee Don-Hwa transcended the modernization theory when he was applying it to answer questions, it seemed that Lee preceded Liang Shu-Ming. Nevertheless, Lee did not demonstrate the keen insight into the spiritual dimension of Western modernization, and his criticism against Western modernization did not touch upon the problem of rationality as Liang did.

Development and Validation of Analytical Method and Antioxidant Effect for Berberine and Palmatine in P.amurense (황백의 지표성분 berberine과 palmatine의 분석법 개발과 검증 및 항산화 효능 평가)

  • Jang, Gill-Woong;Choi, Sun-Il;Han, Xionggao;Men, Xiao;Kwon, Hee-Yeon;Choi, Ye-Eun;Park, Byung-Woo;Kim, Jeong-Jin;Lee, Ok-Hwan
    • Journal of Food Hygiene and Safety
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    • v.35 no.6
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    • pp.544-551
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    • 2020
  • The aim of this study was to develop and validate a simultaneous analytical method for berberine and palmatine, which are representative substances of Phellodendron amurense, and to evaluate the antioxidant activity. We evaluated the specificity, linearity, precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ) of analytical methods for berberine and palmatine using high-performance liquid chromatography. Our result showed that the correlation coefficients of the calibration curve for berberine and palmatine exhibited 0.9999. The LODs for berberine and palmatine were 0.32 to 0.35 µg/mL and the LOQs were 0.97 to 1.06 µg/mL, respectively. The inter-day and intra-day precision values for berberine and palmatine were from 0.12 to 1.93 and 0.19 to 2.89%, respectively. The inter-day and intra-day accuracies were 98.43-101.45% and 92.39-100.60%, respectively. In addition, the simultaneous analytical method was validated for the detection of berberine and palmatine. Moreover, we conducted FRAP and NaNO2 scavenging activity assays to measure the antioxidant activities of berberine and palmatine, and both showed antioxidant activity. These results suggest that P.amurense could be a potential natural resource for antioxidant activity and that the efficacy can be confirmed by investigating the content of the berberine and palmatine.

Optimization of the Indole-3-Acetic Acid Production Medium of Pantoea agglomerans SRCM 119864 using Response Surface Methodology (반응표면분석법을 활용한 Pantoea agglomerans SRCM 119864의 Indole-3-acetic acid 생산 배지 최적화)

  • Ho Jin, Jeong;Gwangsu, Ha;Su Ji, Jeong;Myeong Seon, Ryu;JinWon, Kim;Do-Youn, Jeong;Hee-Jong, Yang
    • Journal of Life Science
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    • v.32 no.11
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    • pp.872-881
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    • 2022
  • In this study, we optimized the composition of the indole-3-acetic acid (IAA) production medium using response surface methodology on Pantoea agglomerans SRCM 119864 isolated from soil. IAA-producing P. aglomerans SRCM 119864 was identified by 16S rRNA gene sequencing. There are 11 intermediate components known to affect IAA production, hence the effect of each component on IAA production was investigated using a Plackett-Burman design (PBD). Based on the PBD, sucrose, tryptone, and sodium chloride were selected as the main factors that enhanced the IAA production at optimal L-tryptophan concentration. The predicted maximum IAA production (64.34 mg/l) was obtained for a concentration of sucrose of 13.38 g/l, of tryptone of 18.34 g/l, of sodium chloride of 9.71 g/l, and of L-tryptophan of 6.25 g/l using a the hybrid design experimental model. In the experiment, the nutrient broth medium supplemented with 0.1% L-tryptophan as the basal medium produced 45.24 mg/l of IAA, whereas the optimized medium produced 65.40 mg/l of IAA, resulting in a 44.56% increase in efficiency. It was confirmed that the IAA production of the designed optimal composition medium was very similar to the predicted IAA production. The statistical significance and suitability of the experimental model were verified through analysis of variance (ANOVA). Therefore, in this study, we determined the optimal growth medium concentration for the maximum production of IAA, which can contribute to sustainable agriculture and increase crop yield.

An Analysis of Swimming Injuries and Their Rehabilitation (근육 골격계의 질환 및 재활분석(수영선수를 중심으로))

  • Kim, Kwi-Baek;Ji, Jin-Gu;Kwak, Yi-Sub
    • Journal of Life Science
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    • v.32 no.4
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    • pp.325-330
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    • 2022
  • While swimming is a very popular competitive sports activity, swimming injuries are unique due to the repetitive nature of the swimming stroke and demanding training programs that can result in upper limb overuse. Therefore, the primary objective of this review was to analyze swimmers' injury areas, injury types by stroke type, and swimming rehabilitation, as well as to discuss safety management for improving swimming performance. In this study, the injuries incurred in swimming events were discussed in the order of upper limb injuries (neck, shoulder, arm, and wrist), lower limb injuries (knee and ankle), and waist injuries. An analysis by stroke type found that shoulder injuries occurred most often with freestyle, backstroke, and butterfly strokes, followed by rotator cuff injury, impingement syndrome, and SLAP (superior labral tear from anterior to posterior) lesions. Knee injuries were associated with the breaststroke, whereas spinal cord injuries occurred with the breaststroke and butterfly stroke. Finally, back injuries were associated with the butterfly stroke. During the freestyle stroke, the shoulder undergoes repetitive overhead movement; hence, shoulder and musculoskeletal pain are the most common and well-documented complaints of swimmers. For safety management, coaches and instructors must ensure that athletes do sufficient warm-up and cool-down exercises to avoid injuries. In case of an injury, they should be familiar with first aid measures so that secondary damage can be prevented with its quick application. In addition, coaches and instructors need to be trained in injury prevention and treatment so that they can provide appropriate rehabilitation treatment for athletes. Although swimming-related injuries cannot be completely eliminated, to reduce them to a minimum, leaders need the knowledge to apply scientific and systematic training principles and methods individualized for each athlete.

Analysis of Central Line-associated Bloodstream Infection among Infants in the Neonatal Intensive Care Unit: A Single Center Study

  • Kim, Minhye;Choi, Sujin;Jung, Young Hwa;Choi, Chang Won;Shin, Myoung-jin;Kim, Eu Suk;Lee, Hyunju
    • Pediatric Infection and Vaccine
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    • v.28 no.3
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    • pp.133-143
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    • 2021
  • Purpose: This study aimed to determine the incidence of central line-associated bloodstream infection (CLABSI) in the neonatal intensive care unit (NICU), evaluate the patients' clinical characteristics, and identify the etiologic agents for guidance in prevention and treatment. Methods: A retrospective chart review study of infants classified as having CLABSI was conducted at the NICU of Seoul National University Bundang Hospital from January 2016 to December 2020. Results: Of the 45 infants, 53 had CLABSIs within a follow-up period of 18,622 catheter days. The incidence of CLABSIs was 2.85 per 1,000 catheter days. The most common catheter type was a peripherally inserted central catheter (n=47, 81%). A total of 57 pathogens were isolated, of which 57.9% (n=33) were Gram-positive bacteria, 36.8% (n=21) were Gram-negative bacteria, and 5.3% (n=3) were Candida spp. The most common pathogens were Staphylococcus aureus (n=12, 21%) and coagulase-negative staphylococci (n=12, 21%), followed by Klebsiella aerogenes (n=8, 14%). The median duration of bacteremia was 2 days, and 19 episodes showed bacteremia for 3 days or more. The mortality rate of infants within 14 days of CLABSI was 13.3% (n=6). Conclusions: This study analyzed the incidence of CLABSI and the distribution of pathogens in the NICU. Continuous monitoring of CLABSI based on active surveillance serves as guidance for empiric antibiotic use and also serves as a tool to assess the necessity for implementation of prevention strategies and their impact.

Selection and Characterization of Antagonistic Microorganisms for Biological Control of Acidovorax citrulli Causing Fruit Rot in Watermelon (수박에 과실썩음병을 유발하는 Acidovorax citrulli의 생물학적 방제를 위한 길항 미생물 선발과 특성 검정)

  • Kim, Ki Young;Park, Hyo Bin;Adhikari, Mahesh;Kim, Hyun Seung;Byeon, Eun Jeong;Lee, In Kyu;Lee, Youn Su
    • Research in Plant Disease
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    • v.28 no.2
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    • pp.69-81
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    • 2022
  • This study was performed to screen the efficacy of antagonistic bacterial isolates from various sources against the bacterial fruit blotch (BFB) causing pathogen (Acidovorax citrulli) in cucurbit crops. In addition, plant growth promoting traits of these antagonistic bacterial isolates were characterized. Two thousand seven hundred ninety-four microorganisms were isolated from the collected samples. Molecular identification revealed two A. citrulli out of 2,794 isolates. In vitro antagonistic results showed that, among the 28 antagonistic bacterial isolates, 24 and 14 bacterial isolates exhibited antagonism against HPP-3-3B and HPP-9-4B, respectively. Antagonistic and growth promotion characterization of the antagonistic bacterial isolates were further studied. Results suggested that, 4 antagonistic bacteria commonly showed both antagonism and growth promotion phenotypes. Moreover, 3 isolates possessed growth promoting activities. Overall results from this study suggests that BFB causing bacterial pathogen (A. citrulli) was suppressed in in vitro antagonism assay by antagonistic bacterial isolates. Furthermore, these antagonistic bacterial isolates possessed growth promotion and antagonistic enzyme production ability. Therefore, data from this study can provide useful basic data for the in vivo experiments which ultimately helps to develop the eco-friendly agricultural materials to control fruit rot disease in cucurbit crops in near future.

Study of Spatiotemporal Variations and Origin of Nitrogen Content in Gyeongan Stream ( 경안천 내 질소 함량의 시공간적 변화와 기원 연구)

  • Jonghoon Park;Sinyoung Kim;Soomin Seo;Hyun A Lee;Nam C. Woo
    • Economic and Environmental Geology
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    • v.56 no.2
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    • pp.139-153
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    • 2023
  • This study aimed to understand the spatiotemporal variations in nitrogen content in the Gyeongan stream along the main stream and at the discharge points of the sub-basins, and to identify the origin of the nitrogen. Field surveys and laboratory analyses, including chemical compositions and isotope ratios of nitrate and boron, were performed from November 2021 to November 2022. Based on the flow duration curve (FDC) derived for the Gyeongan stream, the dry season (mid-December 2021 to mid-June 2022) and wet season (mid-June to early November 2022) were established. In the dry season, most samples had the highest total nitrogen(T-N) concentrations, specifically in January and February, and the concentrations continued to decrease until May and June. However, after the flood season from July to September, the uppermost subbasin points (Group 1: MS-0, OS-0, GS-0) where T-N concentrations continually decreased were separated from the main stream and lower sub-basin points (Group 2: MS-1~8, OS-1, GS-1) where concentrations increased. Along the main stream, the T-N concentration showed an increasing trend from the upper to the lower reaches. However, it was affected by those of the Osan-cheon and Gonjiamcheon, the tributaries that flow into the main stream, resulting in respective increases or decreases in T-N concentration in the main stream. The nitrate and boron isotope ratios indicated that the nitrogen in all samples originated from manure. Mechanisms for nitrogen inflow from manure-related sources to the stream were suggested, including (1) manure from livestock wastes and rainfall runoff, (2) inflow through the discharge of wastewater treatment plants, and (3) inflow through the groundwater discharge (baseflow) of accumulated nitrogen during agricultural activities. Ultimately, water quality management of the Gyeongan stream basin requires pollution source management at the sub-basin level, including its tributaries, from a regional context. To manage the pollution load effectively, it is necessary to separate the hydrological components of the stream discharge and establish a monitoring system to track the flow and water quality of each component.

Metatranscriptome-Based Analysis of Viral Incidence in Jujube (Ziziphus jujuba) in Korea (메타전사체 분석을 이용한 국내 대추나무의 바이러스 감염실태)

  • Hong-Kyu Lee;Seongju Han;Sangmin Bak;Minseok Kim;Jean Geung Min;Hak ju Kim;Dong Hyun Kang;Minhui Kim;Wonyoung Jeong;Seungbin Baek;Minjoo Yang;Taegun Lim;Chanhoon An;Tae-Dong Kim;Chung Youl Park;Jae Sun Moon;Su-Heon Lee
    • Research in Plant Disease
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    • v.29 no.3
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    • pp.276-285
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    • 2023
  • This work investigated the viral infection in jujube plants in Korea. A total of 61 samples with the symptoms of putative viral infection were collected from experimental fields and orchards. Thereafter, the samples were subjected to metatranscriptome analysis, Reverse transcription polymerase chain reaction analysis, and nucleotide sequence analysis. These analyses identified the presence of two DNA viruses, jujube-associated badnavirus (JuBV), jujube mosaic-associated virus (JuMaV), and one RNA virus, jujube yellow mottle-associated virus (JYMaV). All samples collected were confirmed to be infected by at least one of the three viruses, with most showed multiple infections. The detection rates of JuBV, JYMaV, and JuMaV were 100%, 90.2%, and 8.2%, respectively. Only three combinations of viral infections were found: 9.8% of samples showed single infection of JuBV, 82.0% showed double infection of JuBV+JYMaV, and 8.2% showed triple infection of JuBV+JYMaV+JuMaV. Sequence analysis of the three viruses showed very high homology with respective virus isolates reported in China. This study is predicted to provide fundamental data to produce virus-free jujube seedlings and represents the first report of JuBV and JuMaV infection in Korea.

A Study on the Classification of Rural Tourism Resources through a Card Sorting Test -Focused on Rural Amenity Resources Database- (카드분류법을 통한 농촌관광자원 유형 분류 -농촌어메니티자원 DB를 중심으로-)

  • Kang, Young Eun;Park, Mee Jeong;Kim, Sang Bum;Kim, Eun Ja
    • Journal of recreation and landscape
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    • v.6 no.2
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    • pp.63-71
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    • 2012
  • As the interest in rural tourism has been increasing since the late 1990s, the research associated with rural tourism has increased, including research on the classification of rural tourism resources. The research classifying these resources has proved useful to many other studies. Although such studies have been conducted for a long time, they have expressed only experts' perspectives and been supported by statistics, without reflecting on users' opinions. Given this background, this study aims to classify rural tourism resources by focusing on the rural activities for tourists who use those tourism resources. To achieve this, each study participant proceeded to collect tourism resources by using a rural amenity resources database, and a card sorting test was conducted. Thirty-two people who had previously gone sightseeing in the rural areas were chosen as participants in the card sorting test. After the card sorting test was complete, the results were reviewed by experts. These results yielded six categories: doing nature activities, eating and cooking local dishes, putting up (overnight stays), going sightseeing/appreciating the landscape, enjoying leisure activities, and doing artistic activities. In the doing nature activities category, there were four subcategories: experiencing local resources, experiencing nature, experiencing tradition, and harvesting. This study was conducted to improve the satisfaction and understanding of the tourists who visit rural areas. Thus, the classification of rural tourism resources developed by this study will be widely used to establish the framework or contents of websites, applications, and so on, for promoting rural tourism resources and local resources.

Effect of Manganese Sulfate Concentration in Media on Production Speed of Insecticidal Crystal by Bacillus thuringiensis (배지 중 Manganese sulfate 농도가 Bacillus thuringiensis의 곤충독소 생성 시간에 미치는 영향)

  • Ro-Un Lee;Do Gyung Oh;Eun-Sun Jeong;Jung-Beom Kim
    • Journal of Food Hygiene and Safety
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    • v.38 no.3
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    • pp.170-175
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    • 2023
  • In this study, the effect of MnSO4 on the insecticidal crystal (IC) produced by Bacillus thuringiensis for a rapid detection medium was analyzed. The strains used included one B. thuringiensis reference (KCTC 1511) and nine wild-type strains. The IC in B. thuringiensis was detected following the method published by the Ministry of Food and Drug Safety in Korea. In the nutrient agar to which 0.005% MnSO4 was added, IC was observed on two of the three plates after 48 hours of incubation and on all three plates after 120 hours. In AK agar, IC was observed on one and two of the three plates after 48 and 96 hours of incubation, respectively. These results indicated that 0.005% MnSO4 nutrient agar is more appropriate than AK agar for production of IC in B. thuringiensis. The effect of various MnSO4 concentrations on IC production was studied after 24 hours of incubation. IC was produced on 1 of the 10 plates with 0.000% MnSO4 nutrient agar, 2 of the 10 plates with 0.001% MnSO4 nutrient agar, and 3 of the 10 plates with 0.002% MnSO4 nutrient agar. IC was not observed for the other nutrient agars containing 0.003%-0.009% MnSO4. These results indicated that nutrient agar with 0.002% MnSO4 led to the most rapid production of IC by B. thuringiensis after 24 hours of incubation. However, the conditions for IC production by B. thuringiensis depended on the incubation conditions and strain activity. Therefore, further studies are needed to verify the effects of 0.002% MnSO4 on the production of IC by various Bacillus thuringiensis strains.