• Journal of the Korean Applied Science and Technology
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• v.37 no.4
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• pp.906-914
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• 2020

To investigate the usefulness of natural plants, physicochemical characteristics, functional components and physiological activities of dodder(Cuscuta australis R.Brown) were experimented. The extraction of dodder was carried out at 60℃ in shaking bath with 10 times of distilled water for three hours. Harmful metals such as lead, cadmium and arsenic were not detected, but the content of mercury from dodder was 0.0004ppm in acceptable standards. The extraction yield was 23.45%, total content of phenolics equivalent to gallic acid was 699.8 mg/100g and total content of flavonoid equivalent to naringin was 373.8 mg/100g. 28 kinds of free amino acids were detected with the contents of 252mg/100g in the dodder extract, and the contents of anserine and sarcosine were analysed highest in all amino acids. From the comparison of physiological activities of dodder extract with those of ascorbic acid, total reducing power, electron donating ability based on DPPH radical scavenging and ferric reducing antioxidant power was equivalent to 88%, 56% and 40%, respectively, of ascorbic acid. The nitrite scavenging activity of dodder extract was 78% of ascorbic acid on pH 1.5, but the antioxidant index of dodder on perilla oil was about 4 times greater than that of ascorbic acid.

• Journal of the Korean Applied Science and Technology
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• v.39 no.1
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• pp.108-114
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• 2022

In this study, the antioxidant effect of black sea ginseng containing a large amount of eicosapentaenoic acid, a natural physiologically active substance, was investigated, and its superiority as a functional cosmetic raw material was presented. The ability of the sea cucumber extract to remove active oxygen was evaluated for the inhibitory effect on intracellular nitric oxide (NO) production. The physiologically active substances of sea cucumber have an antioxidant effect that removes free radicals, and as a result of evaluation, it was confirmed that excellent NO production was suppressed. Studies have shown that bioactive substances are involved in antioxidant effects, immune and inflammatory response regulation by showing high antioxidant efficacy even at low concentrations. It is thought that the antioxidant removal ability of sea cucumber greatly contributes to the antioxidant efficacy of phenolic components containing EPA (eicosapentaenoic acid) of the sea cucumber extract. It was found to be similar to the report that the antioxidant component of sea cucumber works. In addition, it is excellent for wound healing with the eicosapentaenoic acid (EPA) component contained in a large amount of sea cucumber extract, which has been proven to be involved in the inhibitory effect of inflammatory activity. As a result of this study, it was confirmed that the effect of the phenol component contained in the sea cucumber extract had a close causal relationship with the antioxidant activity.

• Journal of Applied Biological Chemistry
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• v.65 no.1
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• pp.49-55
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• 2022

Biorenovation is a method for converting materials using the enzyme properties of microorganisms. Natural products converted by that method increase physiological activity or reduce cytotoxicity. In this study, we investigated the anti-inflammatory activity of crypsinus hastatus prothallium (CH) and biorenovated CH prothallium (CHB) using RAW 264.7 cells stimulated with lipopolysaccharide (LPS). CHB inhibited the production of nitric oxide, prostaglandin E₂ and cytokines (interleukin-6, interleukin-1β, tumor necrosis factor-α) compared to CH at a concentration of 50-200 ㎍/mL. In addition, CHB concentration of 200 ㎍/mL inhibited the expression of inducible nitric oxide synthase and cyclooxygenase-2 protein by LPS stimulation to the level of the untreated control group. These results indicate that CHB could be a novel anti-inflammatory agent for cosmetic and pharmaceutical ingredients.It also suggests that the application of biorenovation has potential usefulness in developing anti-inflammatory materials. It also suggests that the application of bio-renovation has potential usefulness in the development of inflammatory material. We applied Biorenovation technology to Distylium racemosum extract (DR) to generate Distylium racemosum biorenovation product (DRB), and investigated the anti-inflammatory properties of DRB in lipopolysaccharide (LPS)-treated RAW264.7 macrophages. We are applying technology to Biorenovation Distylium racemosum extract (DR) Distylium racemosum was to create a biorenovation product (DRB), lipopolysaccharide (LPS) investigated the anti-inflammatory properties of DRB in RAW264.7 macrophages treated for.

• Membrane Journal
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• v.28 no.1
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• pp.75-82
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• 2018

Forward osmosis (FO) desalination system has been highlighted to improve the energy efficiency and drive down the carbon footprint of current reverse osmosis (RO) desalination technology. To improve the trade-off between water flux and salt rejection of thin film composite (TFC) desalination membrane, thin film nanocomposite membranes (TFN), in which nanomaterials as a filler are embeded within a polymeric matrix, are being explored to tailor the separation performance and add new functionality to membranes for water purification applications. The objective of this article is to develop a graphene nanocomposite membrane with high performance of water selective permeability (high water flux, high salt rejection, and low reverse solute diffusion) as a next-generation FO desalination membrane. For advances in fabrication of graphene oxide (GO) membranes, layer-by-layer (LBL) technique was used to control the desirable structure, alignment, and chemical functionality that can lead to ultrahigh-permeability membranes due to highly selective transport of water molecules. In this study, the GO nanocomposite membrane fabricated by LBL dip coating method showed high water flux ($J_w/{\Delta}{\pi}=2.51LMH/bar$), water selectivity ($J_w/J_s=8.3L/g$), and salt rejection (99.5%) as well as high stability in aqueous solution and under FO operation condition.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.2
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• pp.280-286
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• 2020

Cards made from PVC and PET materials do not oxidize or decompose readily, so they are generally incinerated or landfilled after use and cause pollution problems, such as environmental hormones and combustion gases during incineration. In addition, there is a problem of environmental pollution because they are discarded as semi-permanent refuse without being decomposed at landfill. This study attempted to solve this problem using polylactic acid (PLA), which is a representative biodegradable material as a substitute material that can solve the issues with these cards. On the other hand, when the thin card core sheet is made from only PLA material, the physical properties of the material are insufficient, such as the low temperature impact strength, high temperature stability, and poor bending properties, so its use is limited. To solve this problem, the compositional ratio of PLA was reviewed, and the optimal biodegradable compound composition was determined through an examination of the compositions, such as crystallization nucleating agents, additives, and nano compound technology. The high functionalization as a biodegradable card was verified through a laminating process using annealing technology.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.8
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• pp.1344-1350
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• 2003

This study was performed to investigate the antioxidative effect as the inhibition of malondialdehyde (MDA) and bovine serum albumin (BSA) conjugation reaction, inhibition of lipid peroxidation and the scavenging effect on 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, and antimutagenic capacities as the Ames test in 7 common vegetables taken by Korean for suggestion of prevention and dietetic treatment of chronic diseases and development of antioxidative and antimutagenic functional food. The water fractions of perilla leaves and sedum were most effective in the inhibition of MDA and BSA conjugation reaction showing 62.5% of inhibition rate among 7 vegetables. The inhibition rates of ethanol fractions of sedum and wild water dropwort on the lipid peroxidation were 67.1% and 61.5%, respectively. The ethanol fractions of crown daisy and wild water dropwort showed the most effective results among 7 vegetables in the DPPH radical scavenging capacities showing inhibition rate of 78.8% and 73.6%, respectively. The indirect and direct antimutagenic effects of ethanol extract of 7 vegetables were examined by Ames test using Salmonella typimurium TA98 and TA100. Inhibitory effects of wild water dropwort was superior to the other vegetables on the Ames test. These results suggest that common 7 vegetables taken by Korean are believed to be a possible antioxidative and antimutagenic capacities, although the results were different, more or less, according to the assay method and vegetables used.

• Food Science of Animal Resources
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• v.22 no.1
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• pp.20-29
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• 2002

The functional sausage added to effective extracts are prepared to carried out to investigate functional and storage characteristics. This products were stored at different temperature. The changes of pH were tended to be a little ranged from pH 6.07 to pH 6.35 in control. At the same time, the pH changes treated with plant extracts showed the same tendency as control. The treatments using natural extracts revealed a little low TBARS value during storage at 10$^{\circ}C$. The nitrite scavenging ability of extracts from pine needle were higher than those of green tea extracts, irrespective of storage temperature. The VBN content was tended to be increased as storage time goes by, irrespective of storage temperature. The treatments using plant extracts revealed a little low VBN content, compared to control during storage. The changes of total bacteria were more increased to 2.2${\times}$10$^1$∼3.2 ${\times}$ 10$\^$6/ CFU/g during storage at 30$^{\circ}C$ than 2.2${\times}$10$^1$∼3.3${\times}$10$^2$CFU/g in case of storage at 10$^{\circ}C$. The treatments using plant extracts revealed an antimicrobial activity until storage at 3 days, compared to control. The lightness of sausage color were a little more decreased gradually during storage at 30$^{\circ}C$ than those of storage at 10$^{\circ}C$. Overall, the lightness of sausage color treated with pine needle extracts were a more bright than those of control. However, the redness of sausage color treated with pine needle and green tea showed the most lowest red color, compared to control. Sensory test suggested that the changes of sausage color, flavor, texture and taste were tended to be decreased gradually. In conclusion, pine needle extract was the most effective natural resources on the basis of the functional and physico-chemical properties of sausage of sausage.

• Proceedings of the Materials Research Society of Korea Conference
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• 2009.05a
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• pp.30.2-30.2
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• 2009

TiO2는 3가지의 결정구조를 가지고 있으며 결정 입자, 구조, 상의 형태에 따라서 성질 및 기능에 영향을 주고 있다. anatase상의 애너지 밴드갭과 전자와의 재결합 확률이 크기 때문에 Rutile상 보다 우수한 성질을 갖고 있어 산화물 반도체로 이용하는 것이 적합하다. 본 실험에서는 나노로드의 TiO2를 수열처리법에 의해 합성한 후 박막을 제조하여 감응특성을 조사하였다. X선 회절분석기(X-Ray Diffraction)로 분석결과 ph=1의 루타일상을 제외하고, pH=2~7의 더 넓은 구간에서 뚜렷한 회절피크의 anatase 상이 나타났으며 다른 비정질상이 발생되지 않는 결정성이 좋은 단결정임이 나타났다. NaOH solution 을 이용하여 수열처리후 $180^{\circ}C$이상의 특정 온도 구간에서 수십 나노 로드 형태로의 2차 성장된 모습을 TEM과 EDS로 결정구조와 화학조성을 분석하였다. 그리고 BET 측정을 통해 $180^{\circ}C$의 소성온도에서 TiO2 입자의 비표면적이 가장 우수한 것으로 나타났다. 나노로드의 수용액을 Al2O3기판의 감지막 위에 떨어뜨려 네트워크된 막을 형성한후에 센서를 제작하였다. 히터 전압이 $400^{\circ}C$에서 나노 파우더센서에서는 반응이 일어나지 않은 반면, 나노 로드센서는 CH3SH에서 28% 의 높은 감도를 얻었고, Toluene의 반응에서는 15%의 감도가 나타났다. 그 외 NO, CO, H2등의 측정에서 아무런 반응이 일어나지 않았다. 이는 비교적 기공이 큰분자(Size)를 가진 CH3SH=76nm, Toluene=60nm에서 반응이 일어난 반면, H2=28nm, CO=22nm에서 감도가 나타나지 않은 것을 보아 흡착분자크기에 의한 영향이 큰 것으로 나타났다.

• Korean Journal of Medicinal Crop Science
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• v.15 no.4
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• pp.296-303
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• 2007

Acer tegmentosum (Acereaceae) has been used a source of traditional medicines for the treatment of hepatic disorders in Korea. This research was conducted to determine biofunctional activities of A. tegmentosum stem extract and to identify its bioactive components. Methanolic extract from A. tegmentosum stem was partitioned by using organic solvents, including n-hexane, ethyl acetate, n-butanol, and water. Two compounds were isolated by using an ODS column chromatography from ethyl acetate soluble fraction shown to the strongest antioxidant activity ($RC_{50}=3.15\;{\mu}g/m{\ell}$) among the fractions. The isolated compounds were analyzed by $^1H$ and $^{13}C$ NMR, IR, UV/VIS, MS spectrum data and identified as catechin, ${\rho}-Hydroxyphenethyl$ alcohol $1-O-{\beta}-_D-(6'-O-galloyl)-glucopyranoside$. The compounds have shown strong antioxidant activity, with similar activity to BHA ($RC_{50}=2\;{\mu}g/m{\ell}$). Especially, ${\rho}-Hydroxyphenethyl$ alcohol 1-O-{\beta}-_D-(6'-O-galloyl)-glucopyranoside$ was shown strong anti-lipid peroxidative activity. However, the compounds were not shown antimicrobial activities. In antimicrobial activity assays, ethyl acetate soluble fraction was effective to bacterial inhibition, such as Escherichia coli and Klebsiella pneumonia, with minimum inhibitory concentrations in $125\;{\mu}g/m{\ell}$. Otherwise, antifungal activity against Candida albicans was shown in n-hexane soluble fraction exhibiting $63\;{\mu}g/m{\ell}$ of minimum inhibitory concentration. In anticomplementary activity assays, water soluble fraction was the most effective exhibiting 24% inhibitory activity.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.7
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• pp.504-509
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• 2018

The purpose of this study was to manufacture a high-performance titanium yellow pigment. Anatase type $TiO_2$ was the skeleton of the pigment and $Sb_2O_3$ is used as the color assistant for the coloring agent, $Cr_2O_3$. Mixed raw materials for the pigment were $TiO_2$(98%), $Sb_2O_3$(99.5%), and $Cr_2O_3$(99.5%). The raw materials were mixed by a dry process and crystallized by calcination at $1,000{\sim}1,200^{\circ}C$. The crystalline material was pulverized in a Jar Mill under $1{\mu}m$ by a wet process and dried for 12 hours at $100^{\circ}C$. The pigment was finally made by a fine grinding process. To determine the best temperature for calcination, 4 temperature sections ($1000^{\circ}C$, $1100^{\circ}C$, $1150^{\circ}C$, and $1200^{\circ}C$) were set up. The X-ray diffraction peak of the rutile crystalline structure was highest at $1,150^{\circ}C$. The yellow ceramic pigment, which has the rutile structure, was applied for coating materials. The synthesized pigments underwent a discoloration tests on the acid resistance, alkaline resistance, weather resistance and heat resistance. In addition, a detection test on harmful heavy metals ($Cr^{+6}$) was done. The resulting values (${\Delta}E$) of the weather resistance test (2000hr), acid resistance test, alkaline resistance test, and heat resistance test were 0.74, 0.16, 0.07 and 0.29. The resulting value for heavy metals testing was 34ppm.