Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Anti-inflammatory effect of Crypsinus hastatus biorenovation extract

고란초 생물 전환 추출물의 항염증 효과

  • Lee, Kyung-Mi (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University) ;
  • Choi, Byeong Min (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University) ;
  • Park, Tae-Jin (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University) ;
  • Hong, Hyehyun (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University) ;
  • Kim, Seung-Young (Department of Pharmaceutical Engineering & Biotechnology, Sunmoon University)
  • Received : 2021.12.30
  • Accepted : 2022.02.18
  • Published : 2022.03.31
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Abstract

Biorenovation is a method for converting materials using the enzyme properties of microorganisms. Natural products converted by that method increase physiological activity or reduce cytotoxicity. In this study, we investigated the anti-inflammatory activity of crypsinus hastatus prothallium (CH) and biorenovated CH prothallium (CHB) using RAW 264.7 cells stimulated with lipopolysaccharide (LPS). CHB inhibited the production of nitric oxide, prostaglandin E2 and cytokines (interleukin-6, interleukin-1β, tumor necrosis factor-α) compared to CH at a concentration of 50-200 ㎍/mL. In addition, CHB concentration of 200 ㎍/mL inhibited the expression of inducible nitric oxide synthase and cyclooxygenase-2 protein by LPS stimulation to the level of the untreated control group. These results indicate that CHB could be a novel anti-inflammatory agent for cosmetic and pharmaceutical ingredients.It also suggests that the application of biorenovation has potential usefulness in developing anti-inflammatory materials. It also suggests that the application of bio-renovation has potential usefulness in the development of inflammatory material. We applied Biorenovation technology to Distylium racemosum extract (DR) to generate Distylium racemosum biorenovation product (DRB), and investigated the anti-inflammatory properties of DRB in lipopolysaccharide (LPS)-treated RAW264.7 macrophages. We are applying technology to Biorenovation Distylium racemosum extract (DR) Distylium racemosum was to create a biorenovation product (DRB), lipopolysaccharide (LPS) investigated the anti-inflammatory properties of DRB in RAW264.7 macrophages treated for.

Biorenovation은 미생물의 효소적 기능을 이용하여 천연 추출물과 같은 소재를 생물전환하는 기법으로 생리활성이 증진되거나 세포독성을 감소시키는 효능이 보고되었다. 이에 본 연구는 천연소재의 생리활성을 증진하고 가치를 향상시키고자 biorenovation 기법을 적용하여 고란초 전엽체 추출물(CH)을 생물전환하였고, LPS로 자극된 RAW 264.7 세포를 사용하여 항 염증 활성을 평가하였다. 세포 사멸을 보이지 않는 50-200 ㎍/mL 농도에서 CH와 생물 전환 추출물(CHB)의 Inducible nitric oxide synthase(iNOS), cyclooxygenase-2 (COX-2)의 발현량 및 전 염증성 사이토카인의 생성량을 조사한 결과, CHB는 CH보다 산화질소, prostaglandin E2 및 사이토카인(interleukin-6, interleukin-1β, tumor necrosis factor-α)의 생성을 억제하였으며, 특히 CHB는 200 ㎍/mL 농도에서 LPS 자극에 의한 iNOS 및 COX-2 단백질의 발현을 무 처리군과 유사한 수준으로 억제하였다. 이러한 결과는 biorenovation 생물 전환을 통해서 기존 식물 추출물의 항염증 활성이 증진될 가능성을 나타내며, CHB가 효과적인 항염증 소재로서 화장품 및 건강기능식품에 적용될 가능성을 시사한다.

Keywords

Acknowledgement

본 연구는 중소벤처기업부와 한국산업기술진흥원이 지원하는 지역특화산업육성+(R&D) 사업으로 수행된 연구결과입니다(S3090174).

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