• Tuberculosis and Respiratory Diseases
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• v.64 no.3
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• pp.194-199
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• 2008

Background: Early identification of pathogens can improve the prognosis of patients with ventilator associated pneumonia (VAP). In the present study, we evaluated the feasibility of performing multiplex PCR for endotracheal aspirates to detect three important pathogens (P. aeruginosa, K. pneumoniae and MRSA) in patients with VAP. Methods: The endotracheal aspirates of 24 patients were collected within 24 hours of the diagnosis of VAP for performing multiplex PCR. Forward and reverse primers were designed to target the specific site of each pathogen (the oprL gene for P. aeruginosa, 16S rRNA for K. pneumoniae and the mec gene for MRSA). We analyzed the clinical data of the VAP patients, including the culture reports for the endotracheal aspirates. Results: Twenty-four patients (M:F=18:6, mean age=$70{\pm}11$) with VAP were enrolled. Pathogens were isolated from 11 patients (P. aeruginosa in 2, K. pneumoniae in 1, MRSA in 2, other enteric Gram negative bacilli in 3, S. pneumoniae in 2 and mixed infection in 1). Multiplex PCR detected three cases of P.aeruginosa (2 cases coincided with the culture reports) and four cases of K. pneumoniae (1 matched with the culture report). PCR detected two MRSA cases, which did not coincide with the culture reports. Conclusion: Multiplex PCR of the endotracheal aspirate showed some ability to detect Gram negative bacilli, although caution is required when interpreting the results.

• Applied Biological Chemistry
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• v.34 no.2
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• pp.142-148
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• 1991

When cultured soybean immature seed on 15th days after flowering, suitable temperature in formation of callus were $24{\sim}27^{\circ}C$, and embryogenic callus(EC) were generated at medium containing NAA with growth regulators, and then, results were subcultured the EC, a plenty of shoots and roots were formed at medium supplemented BA 2mg/1 and IAA 2mg/1, respectively, however when used at medium the same time supplemented BA 2mg/1 and IAA 2mg/1, formation of cullus was energetic, and a symptom of organization was not showed , Total lipid contents include in each cultures were increased at low temperature of cultural conditions as much as possible, but glycolipid, phospholipid, free sterol contents were a little increased at $24{\sim}27^{\circ}C$, and free sterol content was increased at a case of embryogenic structure were generated. In fatty acid compositions in each cultures, the contents of unsaturated fatty acid were plenty in EC, and unsaturation rate was 0.837. Besides, in sterol compositions, cholesterol content was remarkably high in EC than that of other cultures.

• Horticultural Science & Technology
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• v.17 no.2
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• pp.138-140
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• 1999

These studies were carried out to investigate the effects of cold pretreatment, dark condition, and Ficoll on the callus formation and organ differentiation in anther culture of carnation for the purpose of settling the techniques of anther culture of 'Rony' carnation. Diameter of flower buds containing anthers with microspores before or after uniuncleated stage was between 4 and 6 mm. A high percentage of callus formation and responding anthers were achieved by low temperature pretreatment at $4^{\circ}C$ for 7 days. Callus formation was promoted under dark condition. Roots were differentiated from calli formed under darkness. Ficoll didn't promote callus formation but promoted differentiation of shoots and roots.

• Korean Journal of Agricultural Science
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• v.7 no.2
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• pp.65-76
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• 1980

In order to determine the effects of cytokinin and auxin on organ formation from tissue of garlic cloves, leaf blades and basal tissues contained meristem of garlic (Allium sativum L.) cloves harvested in 1979 (old cloves) and 1980 (new cloves) were explanted on a MS medium contained various levels of BA ($N^6$-benzyl amino purine), NAA (naphthalene acetic acid), and 2, 4-D (2, 4-dichlorophenoxyacetic acid). And some of the new cloves were explanted on a media contained BA and NAA after chilling treatment at $4^{\circ}C$ for 10, 20, 30 and 40 days. 1. In a culture of leaf blades of old cloves, shoots were differentiated on a medium supplemented with 2mg/l of BA and NAA. 2. Callus was grown as a quite straw-coloured globular mass on a medium contained 0.2 or 2mg/l 2.4-D. 3. As subcultures of globular calli, shoots and roots were differentiated on a medium contained 2mg/l BA and 0.5 or 1 mg/l NAA, whereas no shoots was shown on a conterol. 4. Shoots were differentiated in a culture of leaf blades of new cloves, but they were not in an old cloves in control, and better effect was shown on a medium contained 2mg/l BA and 1mg/l NAA. However shoots were no differentiated from leaf blades chilled at $4^{\circ}C$ for 30 or 40 days at the same condition. 5. Large numbers of adventitious shoots could be obtained from basal region of garlic cultured on a medium contained 1mg/l BA and 4mg/l NAA, or 2mg/l BA and 2mg/l NAA.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.04a
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• pp.159-159
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• 2005

• The Hankook-Saengyark Bo
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• no.264
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• pp.5-5
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• 2005

섬오갈피' 대량생산 길텄다.(산림과학원, 자생지서 개체 선발 생물반응기 배양) - 천마, 실내 균상재배땐 수확 '4배' - 대추 50$^{\circ}C$로 건조 상품성 가장 좋아(농촌지도기관 주문) - 표고 건조때 솔잎넣어 부패$\cdot$벌레발생 방지

• KSBB Journal
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• v.7 no.2
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• pp.85-91
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• 1992

The role of NAA(1-Naphthaleneacetic acid) and BA(6-Benzyladenine) in the adventitious shoot regeneratlon from Populus leaf segments and changes in the pattern of RNA and protein synthesis were investigated. The adventitious shoot regeneration octured at the basal cut end of Populus leaf segments. This process was effected by many factors, including wounding culture conditions, light and plant growth regulators etc. The highest adventitious shoot regeneration frequency was obtained at $0.01mg/\ell$ NAA with $0.2mg/\ell$ BA. In this condition adventitious shoot starved to regenerate on the 13th day of oullure. The most optimal hormone composition for RNA and protein synthesis was $0.01mg/\ell$ NAA with $0.2mg/\ell$ BA. The content of RNA and protein was greater at the proximal part. In the course of adventitious shoot regeneration, the proteins associated with polar-regeneration appeared at the proximal part of populus leaf segment.

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.69-73
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• 2007

A reliable and effective tissue culture system was established for Perilla frutescens (perilla) using different cultivar, explant source, and growth regulator composition in medium. MS medium supplemented with 2.0 mg/L BA and 0.1 mg/L NAA was maximum at shoot induction. Cotyledon explants formed more shoots than hypocotyl explants. The frequency of plant regeneration through organogenesis was higher (22.8%) than that (6.1%) of somatic embryogenesis. Five genotypes of perilla were screened for the feasibility of shoot regeneration, cotyledon explant of 'Manbaek' showed the highest shoot induction at a frequency of 27.3% among the tested cultivars.

• Korean Journal of Veterinary Research
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• v.19 no.1
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• pp.33-44
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• 1979

Herpes virus인 소의 전염성 비기관염 바이러스와 악성 카탈열 바이러스의 형태(形態)와 그 형성과정(形成過程)(형태발생(形態發生))을 조직배양(組織培養)에서 각기 시험(試驗)하였다. 이 두 종류(種類)의 바이러스는 herpes virus의 특징적(特徵的)인 구조(構造)를 가지나 그 형태발생(形態發生)이 상이(相異)함을 밝혔다. 1. 소의 전염성비기관염(傳染性鼻氣管炎) 바이러스 (IBRV/CT3) 배양에서 그 바이러스의 envelope는 세포질막(細胞質膜)으로부터 유래(由來)됨을 밝혔다. 2. IBRV/CT3 배양에서 envelope는 가진 바이러스는 세포핵내(細胞核內)에는 전혀 나타나지 않았다. 3. 소의 악성(惡性)카탈열(熱) 바이러스(MCFV/C500)의 배양에서 그 바이러스의 envelope는 세포핵막(細胞核膜)으로부터 유래(由來)됨을 밝혔다. 4. 소의 악성(惡性)카탈열(熱) 바이러스(MCFV/C500)에 감염(感染)된 토끼의 폐장(肺臟)에서 얻은 macrophage에 대해 바이러스의 소재(所在)를 추구(追究)한바, 이 바이러스는 macrophage의 세포질내(細胞質內)에만 존재(存在)함을 밝혔다. 그리고 이 바이러스는 탐식(貪食)된 바이러스임을 알았다.

• FLOWER RESEARCH JOURNAL
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• v.17 no.3
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• pp.165-171
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• 2009

This experiment was conducted to extend utility of in-vitro culture material stalk and to mass produce uniform plants in Phalaenopsis. The optimal concentration of hyponex medium and plant hormone were examined. The most effective concentration of the hyponex in the flower stalk tissue culture of Phalaenopsis was $4mg{\cdot}L^{-1}$. The most effective concentration of TDZ and BA on the formation of multiple shoots in Phalaenopsis flower stalk culture was $0.3mg{\cdot}L^{-1}$ and $5mg{\cdot}L^{-1}$, respectively. TDZ was more effective for formation of the shoots and multiple shoots than BA at the basic medium with hyponex $4g{\cdot}L^{-1}$.