Browse > Article
http://dx.doi.org/10.5010/JPB.2007.34.1.069

High Frequency Plant Regeneration from the Cultures of Cotyledon Explants of Perilla (Perilla frutescens L.)  

Kim, Kyung-Min (Department of Environmental Horticulture, College of Life Science and Natural Resources, Sangju National University)
Lee, Hyeon-Suk (Division of Plant Biosciences, Department of Agriculture and Life Sciences, Kyungpook National University)
Publication Information
Journal of Plant Biotechnology / v.34, no.1, 2007 , pp. 69-73 More about this Journal
Abstract
A reliable and effective tissue culture system was established for Perilla frutescens (perilla) using different cultivar, explant source, and growth regulator composition in medium. MS medium supplemented with 2.0 mg/L BA and 0.1 mg/L NAA was maximum at shoot induction. Cotyledon explants formed more shoots than hypocotyl explants. The frequency of plant regeneration through organogenesis was higher (22.8%) than that (6.1%) of somatic embryogenesis. Five genotypes of perilla were screened for the feasibility of shoot regeneration, cotyledon explant of 'Manbaek' showed the highest shoot induction at a frequency of 27.3% among the tested cultivars.
Keywords
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Han TJ, Kim IH, Kim SL, Kim JC, Lim CJ, Jin CD (1999) Organ formation-the formation of adventitious roots, trichomes and calli from leaf segments of Arabidopsis thaliana by naphthaleneacetic acid concentrations and their determination times. Kor J Plant Tiss Cult 26: 211-217   과학기술학회마을
2 Ito M., Kato H, OKa Y, Honda G (1996) A taxonomic study of Japanese wild perilla (Labiatae). J Phytogeogr & Taxon 44: 43-52
3 Suh SK, Park HG (1986) Studies on the anther culture of garlic (AIlium sativum L.) callus formation and plant regeneration. j Kor Soc Hort Sci 27: 89-95
4 Moon JG, Choo BK, Doo HS, Kwon TH, Yanh MS, Ryu JH (2000) Effect of growth regulators on plant regeneration from the cotyledon explant in Oriential Melon (Cucumis melo L.). Kor J Plant Tiss Cult 27: 1-6
5 Jung SH, Yang SK, Kim HK, Chung DS, Cho YS, Kim DH (1999) Changes of RNA and protein during callus induction and plant regenration from Perilla frutescens. Kor J Life Sci 9: 29-34
6 Chaudhury A, Qu R (2000) Somatic embryogenesis and plant regeneration of turf-type bermugagrass: Effect of 6-benzyladenin in callus induction medium. Plant Cell Tiss Org Cult 60: 113-120   DOI
7 Eun JS, Kim YS, Han SK (2000) Plantlet Regeneration by tissue cultures of Cyclaman persicum Mill. Kor J Plant Tiss Cult 27: 479-484
8 Ha KS, Han TJ (2002) Adventitous root formation from cotyledon in soybean (Glycine max L.) cultivars. Kor J Plant Biotech 29: 25-31   DOI   ScienceOn
9 Kim DC, Chung HJ, Min BH, Yang DC (2002) Plant regeneration from explants types and cultivars of Boxthorn (Lycium chinese Mill). Kor J Plant Biotech 29: 15-18   DOI   ScienceOn
10 Kim KH, Lee YH, Kim DH, Park YH, Lee JY, Hwang YS, Kim YH (2004) Agrobacterium-mediated genetic transformation of Perilla frutescens. Plant Cell Rep 25: 361-365
11 Lee CO, Li CH, Lim JD, Yu CY (2004) Regeneration ability in germplasms of Perilla frutescens. Kor J Medicinal Crop Sci 12: 500-507   과학기술학회마을
12 Lee BK, Yu SH, Kim YH, Hur HS, Lee SC and Lee JY (2003) Efficient in vitro shoot regeneraion of perilla (Perilla frutescens) from cotyledon, hypocoty and leaf explants. Kor J Breed 35: 237-240
13 Lee HS, Lee JI, Ryu SN, Hur HS (1994) Effect of low temperature and plant growth regulators on callus induction and plant regeneration in anther culture of perilla. Kor J Breed 26: 345-352
14 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tabacco tissue cultures. Physiol. Plant 15: 473-497   DOI
15 Nitta M, Lee JK, Ohnishi O (2003) Asian Perilla crops and their weedy forms: Their cultivation, utilization and genetic relationships. Economic Bot 57: 245-253   DOI   ScienceOn
16 Ohara K., Tommi U, Sato EF, Yazaki K (2003) Limonene production in tobacco with Perilla limonene synthase cDNA. J Exp Bot 54: 2635-2642   DOI   ScienceOn