Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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High Frequency Plant Regeneration from the Cultures of Cotyledon Explants of Perilla (Perilla frutescens L.)

들깨의 자엽절편배양을 통한 고효율 식물체 재분화

  • Kim, Kyung-Min (Department of Environmental Horticulture, College of Life Science and Natural Resources, Sangju National University) ;
  • Lee, Hyeon-Suk (Division of Plant Biosciences, Department of Agriculture and Life Sciences, Kyungpook National University)
  • 김경민 (상주대학교 생명자원과학대학 환경원예학과) ;
  • 이현숙 (경북대학교 식물생명공학부)
  • Published : 2007.03.31
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Abstract

A reliable and effective tissue culture system was established for Perilla frutescens (perilla) using different cultivar, explant source, and growth regulator composition in medium. MS medium supplemented with 2.0 mg/L BA and 0.1 mg/L NAA was maximum at shoot induction. Cotyledon explants formed more shoots than hypocotyl explants. The frequency of plant regeneration through organogenesis was higher (22.8%) than that (6.1%) of somatic embryogenesis. Five genotypes of perilla were screened for the feasibility of shoot regeneration, cotyledon explant of 'Manbaek' showed the highest shoot induction at a frequency of 27.3% among the tested cultivars.

들깨의 조직배양에서 배지의 생장조절제 조성은 0.1 mgyL의 NAA와 2.0 mg/L의 BA가 혼용된 배지에서 신초형성률이 24.7%로 가장 높게 나타났다. 신초형성에 적합한 배양부위는 자엽이었으며 품종별 신초형성율은 $3.0{\sim}27.3%$로 품종간 차이가 컸고, '만백들깨'의 자엽 배양에서 27.3%의 가장 높은 재분화율을 나타내었다. 조직부위별로는 공시된 모든 품종에서 배축보다는 자엽 조직에서 신초형성율이 높았으며, 신초의 분화형태는 배형성 (6.1%)보다 기관형성 (22.8%)을 경유하는 것이 많았다.

Keywords

References

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  1. The apical bud as a novel explant for high-frequency in vitro plantlet regeneration of Perilla frutescens L. Britton vol.4, pp.3, 2010, https://doi.org/10.1007/s11816-010-0141-4