• Korean Journal of Agricultural Science
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• v.31 no.2
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• pp.97-104
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• 2004

Myostatin is a transforming growth and differentiation factor-${\beta}$ family member that acts as a negative regulator of muscle growth. Previously, mutations in the myostatin gene were known to be related to double muscling phenotypes in cattle. Because myostatin gene is highly related to muscle mass, also meat quality, in cattle, we sequenced whole myostatin mRNA and investigated the SNPs (Single Nucleotide Polymorphisms) in Korean cattle (Hanwoo). The results indicated that Hanwoo had an SNP in nt2385 and this mutation can be a useful marker with further verifications. We also investigated expression patterns of the myostatin gene from various muscle tissues and organs. Northern blotting results indicated that myostatin expression was restricted in muscles with variable expression levels. The results presented here can be used as a valuable information for meat quality related traits and muscle mass in cattle.

• Applied Microscopy
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• v.42 no.1
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• pp.17-26
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• 2012

Histologic and microstructural changes during the postembryonic development of the wolf spider Arctosa kwangreungensis were studied using light and scanning electron microscopy to examine the relationship between a morphological differentiation and behavioral properties. The postembryo with abdominal yolk sac was stayed inactive in the egg case because its muscular and visual systems were not fully developed to a functional level. The first instar spiderlings, developed from the postembryo by a first molting process, started to exhibit its pigmentation on their body cuticles. In particular, undifferentiated cell clusters of central nervous system (CNS) were densely distributed within the cephalothorax, and highly differentiated abdominal ganglion was observed. They had a characteristic visual system looks more like its adult counterpart, and had segmented appendages looks more like the tiny spiders containing well oriented muscular system. After 3rd instar, spiderlings grew more rapidly with accordance to their consistent growth and periodical molting processes. Thus, the relative area of CNS with respect to cephalothorax was gradually decreased, instead a pair of venom glands, musculature, and connectives occupied the residual area. It has been revealed that the early development of spider can be controled by the feeding condition of larval period, since histologic and microstructural differentiations in both appendages and optic system were completed at the second instar. In particular, behavioral properties of the wandering spiders that depend on vision and their running ability were deeply related to physiological differentiation of the microstructural development.

• Journal of Life Science
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• v.22 no.4
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• pp.447-455
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• 2012

Akt plays an important role in a variety of cellular physiologies such as growth, proliferation, and differentiation. In skeletal muscle, Akt has been implicated in regulating regeneration, hypertrophy, and atrophy. In this study, the role of Akt has been examined during skeletal muscle differentiation. Culturing C2C12 myoblasts under low serum (1% horse serum) and high density converted cell morphology from a round shape to an elongated and multi-nucleated shape. Morphological changes were initiated from day 2 of differentiation. In addition, the expression of both myogenin G and myogenin D was elevated from day 2 of differentiation. Skeletal muscle differentiation was abolished by silencing Akt1 or Akt2, but was significantly enhanced by the over-expression of either Akt1 or Akt2. The activation of Akt was observed from day 2 of differentiation and disappeared after day 7. The expression of kruppel-like factor 4 was observed from day 6 of differentiation. Moreover, this expression was blocked in cells silencing either Akt1 or Akt2. In addition, the promoter activity of kruppel-like factor 4 was significantly reduced in cells silencing Akt1 or Akt2. These results suggest that Akt regulates skeletal muscle differentiation through the regulation of kruppel-like factor 4 expression.

• Journal of the Korean Chemical Society
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• v.41 no.8
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• pp.406-413
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• 1997

The endogenous steroids from human urine were simultaneously analyzed by selected ion monitoring method of GC/MS which is currently used for the doping procedure, together with anabolic steroids. The recovery range of this method was 72.33 %∼94.54% and the RSD values of precision and accuracy test were 1.43%∼10.86%, 0.96%∼9.98%, respectively. Using this method steroids profile was investigated in the urine of male volunteers after oral administration of nine anabolic steroids banned by IOC (International Olympic Committee). Urinary endogenous steroids level was varied specifically according to the excretion tendency of the metabolites of anabolic steroids.

• Korean Journal of Poultry Science
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• v.48 no.4
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• pp.255-265
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• 2021

Global warming and scorching summer seasons affect the growth ability of broilers and animal welfare. In modern broilers, vital organs, such as the heart and lungs, grow disproportionally under intensive selection, making it difficult to adapt to warmer climates. Changes in environmental temperature can affect muscle formation during embryonic development and the early posthatching period. Satellite cells are highly sensitive to heat stress. Heat stress affects the proliferation and differentiation activity of satellite cells and muscle growth and structure. Therefore, thermal manipulation during broiler chick embryogenesis and environmental temperature management at the beginning of hatching are critical for the development and growth of broiler muscles. This review focuses on the thermoregulation mechanism of birds, the muscle development process of broilers, and the function of satellite cells, the relationship between heat stress and muscle development of chicks shortly after hatching, and studies on heat resistance and muscle growth of broilers.

• Proceedings of the Korean Society of Veterinary Pathology Conference
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• 2002.11a
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• pp.51-60
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• 2002

인간이라는 개체는 한 개의 수정란에서 발생을 시작하여 개체의 크기에 따라 60조에서 100조개 정도의 세포로 구성되며, 각 세포는 모두 동일한 유전자를 가지고 있다. 인간의 염색체에 존재하는 유전자의 염기서열이 밝혀진 현재, 세포의 고유 기능을 유지하기 위하여 발현되는 유전자는 약35,000개 정도로 알려져 있다. 그러나 장기 또는 조직을 이루는 세포들은 그 조직에 따라 발현되는 유전자가 서로 다르기 때문에 상피세포, 혈액세포, 근육세포 등 모양과 기능이 다른 210여 가지의 세포로 분화되어 있다. (중략)

• Journal of Animal Science and Technology
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• v.45 no.2
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• pp.169-182
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• 2003

To understand the structure and regulation of bovine ADRP (Adipocyte Differentiation Related Protein) gene, we have isolated the genomic clone of bovine ADRP and determined its sequence. A genomic Southern blot analysis confirmed that ADRP gene is present as a single copy in bovine genome and the ADRP gene spans 12 kb. Bovine ADRP genomic clone, HwADRPg-1, had 8 exons and 7 introns, and all splicing sites conformed to the GT/AG rule with the exon-intron boundaries located exactly. Analysis of the upstream 649 bp of the sequence of HwADRPg-1 showed that it does not contain any canonical TATAA boxes; however Sp1 binding sites and CAAT boxes are found. The promoter contained potential binding sites for AP-1, AP-2 and several putative transcription factor binding sites. The 5'-flanking region of HwADRPg-1 contained muscle specific transcription activator Myo G and C/EBP (CCAAT/ enhancer binding protein) recognizing site. These results suppose that the Myo G transcription activator regulate the transcription of bovine ADRP gene in muscular tissue and its transcriptional activity was triggered by degree of muscular development. Our results provide the necessary analysis for other flanking sequences are needed in addition to the proximal cis elements of this promoter to confer adipocyte differentiation-dependent or growth-dependent transcriptional control.

• The Journal of the Korean bone and joint tumor society
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• v.11 no.1
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• pp.40-45
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• 2005

Purpose: To suggest an accurate diagnosis and treatment of infiltrating intramuscular lipoma by analysis of the clinical, biological, radiological and pathological features. Materials & Methods: 20 patients who treated at our hospital for infiltrating intramuscular lipoma from 1998. to 2001 were selected for this study. Mean age was 45.8 years old. Four were male and eight female. All cases were checked preoperative radiographs, MRI and diagnosed by biopsy. The methods of surgical treatment included excision of tumor and peripheral tissue. We assessed the recurrence by follow up. Results: Tumors located in upper limbs 5 cases, lower limbs 3 cases, abdomen 3 cases, gluteal region 1 case. In preoperative radiographs, infiltrating intramuscular type were 7 cases. In 11 cases, tumors were completely excied with peripheral tissue. 1 cases was incompletely excised because it was very huge mass and infiltrated lung. Encapsulated tumors were 3cases and uncapsulated tumors 9 cases. There were no recurrence excepts 1 case that was infiltrated lung. Conclusion: Infiltrating intramuscular lipoma was wrongly diagnosed as well differentiated liposarcoma. To increase the rate of correct diagnosis, preoperative radiographs, MRI and pathologic diagnosis were performed. Careful wide excision is necessary to prevent the recurrence.

• Journal of Life Science
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• v.23 no.1
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• pp.137-142
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• 2013

Human adipose-derived mesenchymal stem cells (hMSCs) are highly useful for vascular regeneration of injured or inflamed tissue. Lipopolysaccharide (LPS) is a potent activator of macrophages and stimulates macrophages to release inflammatory cytokines. In the present study, we explored the role of LPS-activated macrophages in the differentiation of hMSCs to smooth muscle cells (SMCs). We demonstrated that conditioned medium from LPS-induced macrophages (LPS CM) stimulates differentiation of hMSCs to SMCs, as evidenced by increased expression of smooth muscle-specific markers, including alpha-smooth muscle actin (${\alpha}$-SMA), smooth muscle-myosin heavy chain, and calponin. LPS induced the secretion of $PGF2{\alpha}$ from macrophages, and $PGF2{\alpha}$ treatment stimulated expression levels of SMC-specific markers in hMSCs. Furthermore, small interfering RNA-mediated silencing of the $PGF2{\alpha}$ receptor inhibited LPS CM-stimulated ${\alpha}$-SMA expression. These results suggest that LPS-activated macrophages promote differentiation of hMSCs to SMCs through a $PGF2{\alpha}$-dependent mechanism.

• Journal of Life Science
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• v.25 no.10
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• pp.1098-1102
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• 2015

FABPpm (plasma membrane-bound fatty acid binding protein ) is highly expressed in skeletal muscle. The principal role of this protein is modulating fatty acid uptake and metabolism. The influence of insulin-like growth factor-I (IGF-I), which is a major regulator of skeletal muscle cells, on FABPpm in skeletal muscle cells has not been investigated. To determine the effect of IGF-I on the expression of FABPpm, differentiated C2C12 murine skeletal muscle cells were treated with 20 ng/ml of IGF-I for different times. IGF-I increased the expression of FABPpm in a time-dependent manner. The mRNA level of FABPpm was measured by real-time quantitative PCR to determine whether the IGF-1-induced induction of FABPpm was regulated pretranslationally. The IGF-I treatment resulted in very rapid induction of the FABPpm mRNA transcript in the C2C12 myotubes. After 24 and 48 hr of the IGF-I treatment, FABPpm mRNA increased 130 and 179%, respectively. The increase in the protein expression returned to control levels after 72 hr of the IGF-I treatment, suggesting that IGF-1 regulated the FABPpm gene pretranslationally in skeletal muscle cells. This is the first evidence that IGF-I has a modulatory effect on the expression of FABPpm. In conclusion, IGF-I induced rapid transcriptional modification of the FABPpm gene in C2C12 skeletal muscle cells and exerted modulatory effects on FABPpm.