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http://dx.doi.org/10.5187/JAST.2003.45.2.169

Genomic Organization and Characterization of the Promoter Region of Bovine ADRP (Adipocyte Different Related Protein) Gene  

Jang, Y. S. (National Livestock Research Institute, R.D.A.)
Yoon, D. H. (National Livestock Research Institute, R.D.A.)
Kim, T. H. (National Livestock Research Institute, R.D.A.)
Cheong, I. C. (National Livestock Research Institute, R.D.A.)
Jo, J. K. (Department of Animal Science, Kyungpook National University)
Publication Information
Journal of Animal Science and Technology / v.45, no.2, 2003 , pp. 169-182 More about this Journal
Abstract
To understand the structure and regulation of bovine ADRP (Adipocyte Differentiation Related Protein) gene, we have isolated the genomic clone of bovine ADRP and determined its sequence. A genomic Southern blot analysis confirmed that ADRP gene is present as a single copy in bovine genome and the ADRP gene spans 12 kb. Bovine ADRP genomic clone, HwADRPg-1, had 8 exons and 7 introns, and all splicing sites conformed to the GT/AG rule with the exon-intron boundaries located exactly. Analysis of the upstream 649 bp of the sequence of HwADRPg-1 showed that it does not contain any canonical TATAA boxes; however Sp1 binding sites and CAAT boxes are found. The promoter contained potential binding sites for AP-1, AP-2 and several putative transcription factor binding sites. The 5'-flanking region of HwADRPg-1 contained muscle specific transcription activator Myo G and C/EBP (CCAAT/ enhancer binding protein) recognizing site. These results suppose that the Myo G transcription activator regulate the transcription of bovine ADRP gene in muscular tissue and its transcriptional activity was triggered by degree of muscular development. Our results provide the necessary analysis for other flanking sequences are needed in addition to the proximal cis elements of this promoter to confer adipocyte differentiation-dependent or growth-dependent transcriptional control.
Keywords
Bovine; ADRP; Promoter; Genomic structure; Myo G; C/EBP;
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