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http://dx.doi.org/10.5352/JLS.2013.23.1.137

Role of LPS-activated Macrophages in the Differentiation of Mesenchymal Stem Cells into Smooth Muscle Cells  

Lee, Mi Jeong (Department of Physiology, School of Medicine, Pusan National University)
Do, Eun Kyoung (Department of Physiology, School of Medicine, Pusan National University)
Kim, Jae Ho (Department of Physiology, School of Medicine, Pusan National University)
Publication Information
Journal of Life Science / v.23, no.1, 2013 , pp. 137-142 More about this Journal
Abstract
Human adipose-derived mesenchymal stem cells (hMSCs) are highly useful for vascular regeneration of injured or inflamed tissue. Lipopolysaccharide (LPS) is a potent activator of macrophages and stimulates macrophages to release inflammatory cytokines. In the present study, we explored the role of LPS-activated macrophages in the differentiation of hMSCs to smooth muscle cells (SMCs). We demonstrated that conditioned medium from LPS-induced macrophages (LPS CM) stimulates differentiation of hMSCs to SMCs, as evidenced by increased expression of smooth muscle-specific markers, including alpha-smooth muscle actin (${\alpha}$-SMA), smooth muscle-myosin heavy chain, and calponin. LPS induced the secretion of $PGF2{\alpha}$ from macrophages, and $PGF2{\alpha}$ treatment stimulated expression levels of SMC-specific markers in hMSCs. Furthermore, small interfering RNA-mediated silencing of the $PGF2{\alpha}$ receptor inhibited LPS CM-stimulated ${\alpha}$-SMA expression. These results suggest that LPS-activated macrophages promote differentiation of hMSCs to SMCs through a $PGF2{\alpha}$-dependent mechanism.
Keywords
Mesenchymal stem cells; lipopolysaccharide; macrophages; smooth muscle cells; alpha-smooth muscle actin (${\alpha}$-SMA);
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