• The Korean Journal of Mycology
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• v.8 no.1
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• pp.33-43
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• 1980

Thirteen strains or crosses of Lentinus edodes obtained from home and abroad were compared for their mycelial characteristics on artificial media. Eleven of them were selected and inoculated on logs in order to study for their productivity, and the morphological characteristics of the mushrooms were investigated. 1) Seven strains, i. e., 11001, 12001, 13005, 13012, 13015, 12004 and 20101 were grown well on the sawdust medium. 2) The best mycelial growth and establish­ment of infection on logs were obtained from strains 20101 and 11001. 3) The highest yield of the mushrooms on logs during the six year periods were obtained from strains 11001, 12001 and 20101. 4) The highest yield of the mushrooms usually was obtained on the fourth year after inoculation. 5) The fruit body productivity in terms of dry weight recovery was the highest in strains 13005 and 20101. 6) The most productive period of the mushrooms during the years depended on strains. Most prevalent types of the strains were summer and autumn growing types, and more mushrooms were produced during these periods. 7) Morphological characteristics of the mushroom were more or less dependent on environmental conditions. 8) Thicker fruit bodies and relatively uniform shapes of the mushroom were produced by strain 20101.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.3
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• pp.302-309
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• 1987

One hundred rice varieties were tested for their level of resistance to sheath blight disease at adult plant stage in field condition through 1984 to 1986. Rice plants were grown under ordinary seasonal culture and inoculated by k-2 fungus isolate during three years. k -1 isolate was also inoculated separately in 1984 and test under late seasonal culture was conducted in separate field in 1985. Degree of damage by the disease observed at 25 days after heading was used to identify the level of resistance of the rice varieties tested. Varietal differences of degree of damage were significant in five tests during three years, and the genotypic variance of degree of damage was always higher than environmental variance among varieties tested. Positive correlations between testing years, between cultural seasons, and between isolates inoculated were found in degree of damage of varieties tested for two or three years continuously. Degree of damage by the disease was correlated negatively with heading date of rice varieties except 1984 tests. Thus, the level of resistance should be compared among the variety group having almost same heading date in field condition, and late and extremely late variety groups should be tested for their level of resistance under appropriate environmental condition. Gayabyo, an early heading variety, and SR9713-54-3, a medium heading breeding line, showed consistent lower value of degree of damage during two or three years. These two varieties were selected as moderate resistant germplasm.

• Journal of Mushroom
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• v.13 no.3
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• pp.151-156
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• 2015

Cordyceps militaris is being studied and cultivated as a medicinal mushroom having many valuable biological and pharmaceutical activities. In the breeding of new C. militaris mushroom, single ascospores were isolated and examined their mycelial growth, mycelial density, and production of stroma and perithecia. Among them selected isolates were crossed and hybrids were produced showing high quality fruiting bodies on artificial media. Mycelial growth rate of new strain 'Dowonhongcho' was higher than that of better on 'Yedang 3' on SDAY at $10-25^{\circ}C$. The stromata of new strain were club-shaped and bright orange-red. Its height was 6.1 cm and the cordycepin content was 0.34% on average. The new strain showed 9% higher yield than 'Yedang 3' with producing firmer fruit bodies. The optimum temperature for mycelial growth was $22{\sim}25^{\circ}C$ and the optimum temperature for stroma development was $18{\sim}22^{\circ}C$. Fruiting bodies were began to produce 45 days later after inoculation. This new cultivar may serve as a valuable one for artificial cultivation and industrial-scale production of C. militaris.

• Journal of Mushroom
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• v.11 no.4
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• pp.244-248
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• 2013

"Hwaseong 5ho" was developed by the method of Di-mon mating between monokaryotic strains derived from "Hwaseong 1ho" and dikaryotic strain "PSC109". The color of pileus was dark grayish brown, the shape of pileus was convex or infundibuliform. The length of stipe was longer and the thickness of stipe was some thinner than Suhan 1ho. Material properties of stipe of "Hwaseong 5ho" was higher in strength, hardness, chewingness and brittleness than Suhan 1ho, but similar in elasticity and cohesion. RAPD using URP-primer showed not the same between two strains. Days of primordia formation period were 22-27 days after spawning, that was a little later than Suhan 1ho. In the trial using culture box containing composted cotton waste, yield index of 'Hwaseong 5ho' was 16.6% higher than Suhan 1ho. The farm field trial were showed stable productivity in each different growing conditions.

• Journal of Animal Science and Technology
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• v.50 no.4
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• pp.509-518
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• 2008

This experiment was conducted to investigate enzyme activity, antimicrobial activity, and antibiotics susceptibility of Lactobacilli strain(Lactobacillus reuteri BLA5, Lactobacillus crispatus BLA7, Lactobacillus reuteri BLA9, Lactobacillus amylovorus LLA7, Lactobacillus crispatus LLA9, Lactobacillus vaginalis LLA11) isolated from chicken ceca and were selected by organic acid synthesis, acid tolerance, bile salt tolerance. The enzymes activities were different among strains of Lactobacilli. The amylase activity and lipase activity of Lactobacillus were high but cellulase activity and protease activity of that were low. Lactobacillus culture showed high antimicrobial activity against E. coli but low antimicrobial activity against Salmonella. The inhibitory factor of Lactobacilli isolated from chickens’ cecum on E. coli was low pH by organic acid. All of Lactobacillus isolated from chicken’s cecum were susceptible to ampicillin and amoxicillin but weren’t susceptible at the optimum level of feed additive antibiotics(virginiamycin and salinomycin).

• Korean journal of applied entomology
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• v.7
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• pp.69-75
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• 1969

These studies aimed to investigating the pathogenicity of Fusarium isolates from the wilted cucur bitaceous plants and the pathogenicity of 24 isolates to cucumber, oriental cantaloup, oriental pickling melon, sponge gourd. muskmelon and watermelon were as follows. 1) The isolates from wilted watermelon and melon severely and one of the isolates was slightly pathogenicon oriental pickling melon or oriental cantaloup. 2) The isolates from wilted cucumber were four types. some of the isolates were severely pathogenic on cucumber and infected oriental cantaloup. oriental pickling melon and melon. Some of the isolates were not pathogenic or slightly pathogenic on cucumber. but these were severely pathogenic on oriental pickling melon. One of the isolates was severely pathogenic on oriental cantaloup oriental pickling melon and melon, but no pathogenic on cucumber and one of the isolates was also severely pathogenic on oriental cantaloup. oriental pickling melon and melon and moderatly pathogenic' on water melon. 3) The isolates from wilted oriental cantaloup were four types some of the isolates infected oriental cantaloup, oriental pickling melon and melon severely and some of the isolates were pathogenic on oriental cantaloup. oriental pickling melon. melon and watermelon. One of the isolates, was slightly pathogenic on oriental pickling melon and melon but one of the isolates was highly pathogenic on melon and infected watermelon slightly. 4) Wilt Fusarium of the cucurbitaceous plants could divide into the group of wilt Fusarium of watermelon, cucumber and muskmelon according to the pathogenicity but it will have to rearrange to one form species from several form species of wilt Fusarium of the cucurbitaceous plants and ought to divide to races according to pathogenicity on severely fixed differential varieties which selected from the cucurbitaceous plants. because of the degree of pathognicity and host range are not surely fixed between isolates of wilt Fusarium of the cucurbitaceous plants tested.

• Microbiology and Biotechnology Letters
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• v.38 no.1
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• pp.32-39
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• 2010

This study was carried out to investigate the usefulness of the natural antimicrobial substances for natural dyeing. Antimicrobial activity of natural substances, extracted by water, ethanol, methanol and ethyl acetate, were shown over 20 mm by Rheum palmatum, Caesalpinia sappan, Prunus mume, Schizandra chinensis, Rhus javanica, and Coptis japonica. Especially, water and methanol extract of C. japonica were shown strong antimicrobial activity against all investigated strains. Minimum inhibition concentration of C. japonica water extract was investigated 110 mg/mL for Staphylococcus aureus ATCC 6538. Investigated natural substances were not only shown strong antimicrobial activity for natural dyeing but also might be used other industries.

• Food Science and Preservation
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• v.20 no.5
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• pp.727-734
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• 2013

The antifungal activity against cheese fungi by lactic acid bacteria isolated from kimchi was investigated. Eight fungi were isolated from cheese in the cheese ripening room. Two of them were identified as Penicillium and Cladosporium via ITS-5.8S rDNA analysis. Twenty-two species of lactic acid bacteria with antifungal activity were isolated from kimchi. Two of them were identified as Lactobacillus and Pediococcus via 16S rRNA sequence analysis. Of the 22 lactic acid bacteria species, six were selected (L. sakei subsp. ALJ011, L. sakei subsp. ALI033, L. sakei subsp. ALGy039, P. pentosaceus ALJ015, P. pentosaceus ALJ024 and P. pentosaceus ALJ026) due to their higher activity against the eight fungi isolated from cheese in the cheese ripening room; and among the six species, the P. pentosaceus ALJ015 and P. pentosaceus ALJ024 isolates from the Jeonju area kimchi and the L. sakei subsp. ALI033 isolate from the Iimsil area kimchi had higher antifungal activity than the other lactic acid bacteria. The minimum inhibitory concentration (MIC) of L. sakei subsp. ALI033 against the eight fungi isolated from cheese in the cheese ripening room was $62.5{\mu}g/mL$.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.10
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• pp.1151-1157
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• 2017

This study examined the immune response of Philippines eel (Anguilla bicolor) to the oral administration of fermented Sparassis crispa stipe extract for 6 weeks. The S. crispa extract fermented with Lactobacillus plantarum showed a higher total phenol content (301.68 ppm) and DPPH radical scavenging activity (63.9%) than those fermented with other strains. Therefore, L. plantarum was selected as a suitable starter culture for the fermentation of S. crispa stipe. The eels were fed a commercial diet supplemented with 1% of fermented S. crispa stipe extract for 6 weeks. The mortality rate of the eels fed the supplemented diet was significantly lower than those of the control after 6 weeks. The lysozyme activity of the serum was increased significantly (12.33 ${\rightarrow}$ 54.66 units) after 6 weeks in the eel fed supplemented diets of fermented S. crispa stipe. The serum of the eel fed the supplemented diet of the S. crispa stipe extract showed higher bactericidal activity. These results suggest that both the S. crispa stipe extract and fermented S. crispa stipe have strong potential to activate the innate immune response of the Philippines eel.

• Journal of Life Science
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• v.15 no.4 s.71
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• pp.513-521
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• 2005

A bacterium producing five fibrinolytic isozymes was isolated from black bean chung kuk. The bacterium was identified as Bacillus subtilis BB-1 by 16s rDNA sequence homology search. A gene out of five fibrinolytic genes in the Bacillus subtilis BB-1 was cloned by shot-gun method. A Cla I DNA fragment of B. subtilis BB-1 chromosome was cloned in to pBluescript II SK(-) and showed the fibrinolytic activity to bacterial cells. The Cla I DNA fragment was sequenced and the sequences did not show homology with gene for protease or fibrinolytic enzyme genes in other organisms. The Cla I DNA fragment was reduced to 2,142 bp by activity-guided PCR cloning method. The optimum pH and temperature of the enzyme were 5.0 and $35^{\circ}C$, respectively. Substrate specificity of the fibrinolytic enzyme was detected in skim milk, casein, gelatin and blood agar plates. The activity of the enzyme was not detected with these substrates. Taken together, this enzyme is a new fibrinolytic enzyme and may be used to prevent thrombosis and arteriosclerosis.