Browse > Article
http://dx.doi.org/10.14480/JM.2015.13.3.151

Varietal characteristics of cross-bred Cordyceps militaris 'Dowonhongcho'  

Lee, Byung-joo (Crop Research Division, Chungcheongnam-do Agricultural Research & Extension Services)
Lee, Mi-Ae (Crop Research Division, Chungcheongnam-do Agricultural Research & Extension Services)
Kim, Yong-Gyun (Crop Research Division, Chungcheongnam-do Agricultural Research & Extension Services)
Lee, Kwang-won (Crop Research Division, Chungcheongnam-do Agricultural Research & Extension Services)
Choi, Young-sang (Yedang Mushroom Institute)
Lee, Byung-eui (Department of Chemistry, College of Natural Science, Soonchunghyang University)
Publication Information
Journal of Mushroom / v.13, no.3, 2015 , pp. 151-156 More about this Journal
Abstract
Cordyceps militaris is being studied and cultivated as a medicinal mushroom having many valuable biological and pharmaceutical activities. In the breeding of new C. militaris mushroom, single ascospores were isolated and examined their mycelial growth, mycelial density, and production of stroma and perithecia. Among them selected isolates were crossed and hybrids were produced showing high quality fruiting bodies on artificial media. Mycelial growth rate of new strain 'Dowonhongcho' was higher than that of better on 'Yedang 3' on SDAY at $10-25^{\circ}C$. The stromata of new strain were club-shaped and bright orange-red. Its height was 6.1 cm and the cordycepin content was 0.34% on average. The new strain showed 9% higher yield than 'Yedang 3' with producing firmer fruit bodies. The optimum temperature for mycelial growth was $22{\sim}25^{\circ}C$ and the optimum temperature for stroma development was $18{\sim}22^{\circ}C$. Fruiting bodies were began to produce 45 days later after inoculation. This new cultivar may serve as a valuable one for artificial cultivation and industrial-scale production of C. militaris.
Keywords
Cordyceps militaris; Fruiting body; Single ascospore; Stroma development;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Che ZM, Wang Y, Zhou LL, Tang CL. 2004. Study on the breeding of a new variety of Cordyceps militaris by mutated with ultraviolet radiation. Food Ferment Ind 30:35-38.
2 Choi IY, Choi JS, Lee WH, Yu YJ, Jong GT, Ju IO, Choi YK. 1999. The condition of production of artificial fruiting body of Cordyceps militaris. Kor J Mycol 27:243-248
3 Choi YS, Kim HK, Lee BJ, Kim YG. 2009. Characteristics and breeding of a new variety Cordyceps militaris 'Yedang 3'. J Mushroom Sci Pro 7:182-186.
4 Cunningham KG, Manson W, Spring FS, Hutchison SA. 1950. Cordycepin, a metabolic product isolated from culture of Cordyceps militaris (Linn.) Link. Nature 166:949-952.
5 Cunningham KG, Hutchinson SA, Nanson W, Spring FS. 1951. Cordycepin, a metabolic product from cultures of Cordyceps militaris (Linn.) Link. Part I. Isolation and characterization. J Chem Soc 1951:2299-3200.
6 Das SK, Masuda M, Sakurai A, Sakakibara M. 2010. Medicinal uses of the mushroom Cordyceps militaris: current state and prospects. Fitoterapia 81:961-968.   DOI   ScienceOn
7 De Julian-Ortiz JV, Galvez J, Munoz-Collado C, Garcia-Domenech R, Gimeno-Cardona C. 1999. Virtual combinatorial syntheses and computational screening of new potential anti-herpes compounds. J Med Chem 42:3308-3314.   DOI   ScienceOn
8 Dong JZ, Lei C, Ai XR, Wang Y. 2012. Selenium enrichment on Cordyceps militaris Link and analysis on its main active components. Appl Biochem Biotechnol 166:1215-1224.   DOI   ScienceOn
9 Du AL, Zhang X, Zhang HZ. 2010. A new high cordycepin Cordyceps militaris cultivar 'Haizhou 1'. Acta Hortic Sin 37:1373-1374.
10 Gao XH. 2008. Mating system of Cordyceps militaris. Acta Edulis Fungi 15:6-10.
11 Gu DX, Zhang GR, Wang JH, Liu X. 2006. A reiview and prospect on the studies of Cordyceps sinensis (Berk) Sacc. J Chin Inst Food Sci Technol 6:137-141.
12 Gui ZZ, Zhu YH. 2008. Advance on cultivation, bioactive compound and pharmacological mechanism of Cordyceps militaris. Sci Seri 34:178-184.
13 Guo, HP, Yang ZM. 1999. Progress in research of pharmacological of Cordyceps sinensis. Traditional Herbal Drugs 30:231-233.
14 Harada Y, Akiyama N, Yamamoto K, Shirota Y. 1995. Production of Cordyceps militaris fruit body on artificially inoculated pupae of Mamestra brassicae in the laboratory. Trans Mycol Soc Japan 36:67-72.
15 Hawksworth DL, Rossman AY. 1997. Where are all the undescribed fungi? Phytopathol 87:888-891.   DOI   ScienceOn
16 Isaka M, Kittakoop P, Kirtikara K, Hywel-Jones, NL, Thebtaranonth Y. 2005. Bioactive substances from insect pathogenic fungi. Acc Chem Res 38:813-823.   DOI   ScienceOn
17 Holliday J, Cleaver M. 2008. Medicinal value of the caterpillar fungal species of the genus Cordyceps (Fr.) Link (Ascomycetes): A review. Int J Med Mushroom 10:219-234.   DOI
18 Huang L, Li QZ, Chen YY, Wang XF, Zhou, XW, 2009. Determination and analysis of cordycepin and adenosine in the products of Cordyceps spp. Afr J Microbiol Res 3:957-961.
19 Huang LF, Liang YZ, Guo FQ, Zhou ZF, Cheng BM. 2003. Simultaneous separation and determination of active components in Cordyceps sinensis and Cordyceps militaris by LC/ESI-MS. J Pharm Biomed Anal 33:1155-1162.   DOI   ScienceOn
20 Jeong JW, Jin CY, Kim MO, Lee JY, Choi YH, Lee JD. 2009. RAPD analysis and cordycepin concentration of hybrided Cordyceps militaris strains by mating. Kor J Mycol 37:86-90.   DOI   ScienceOn
21 Jiang Y, Yao YJ. 2003. Anamorphic fungi related to Cordyceps sinensis. Mycosystema 22:161-176.
22 Lee BJ, Lee MA, Kim YG, Lee KW, Choi YS, Lee BE, Song HY. 2013. Cultural characteristics of Cordyceps militaris strain 'Yedang 3' on various media and nutritional conditons. J Mushroom Sci Prod 11:124-130.   DOI
23 Li J, Chen GS, Fang QM, Li HS. 2010. Comparative study on cultivated Cordyceps militaris and wild Cordyceps sinensis. J Chengdu Univ Trad Chin Med 33:82-84.
24 Liang ZQ. 2001. Current situation and ponderation of Cordyceps Fr. research and exploitation in China. Acta Edulis Fungi 8:53-62.
25 Ling JY, Sun YJ, Zhang H, Lv, P, Zhang CK. 2002. Measurement of cordycepin and adenosine in stroma of Cordyceps sp. by capillary zone electrophoresis (CZE). J Biosci Bioeng 94:371-374.   DOI   ScienceOn
26 Masuda M, Urabe E, Honda H, Sakurai A, Sakakibara M. 2007. Enhanced production of cordycepin by surface culture using the medicinal mushroom Cordyceps militaris. Enzyme Microbiol Tecnnol 40:1199-1205.   DOI   ScienceOn
27 Liu XM, Cheng YH, Tian D. 1999. Progress in the research of pharmacological Cordyceps in China. Natl Product Res Development 11:87-91.
28 Ma T, Feng Y, Wu XP, Zhang YH, Ma Y, Wang ZL. 2007. Primary investigation of a host insect of Cordyceps militaris and analysis of its main ingredients. For Res 20:63-67.
29 Mains EB. 1958. North American entomogenous species of Cordyceps. Mycologia 50:169-222.   DOI
30 Oh SW, Kim SH, Song HN, Han DS. 2003. Comparative chemical compositions of four kinds of Tochukaso. Kor J Food Sci Technol 35:15-22.
31 Patel KJ, Ingalhalli RS. 2013. Cordyceps militaris (L.:Fr.) Link - An important medicinal mushroom. J Pharm Phytochem 2:315-319.
32 Pathania P, Sagar A. 2014. Studies on the biology of Cordyceps militaris: A medicinal mushroom from North West Himalaya. Kavaka 43:35-40.
33 Paterson RRM. 2008. Cordyceps - A traditional Chinese medicine and another fungal therapeutic biofactory? Phytochem 69:1469-1495.   DOI   ScienceOn
34 Sato H, Shimazu M. 2002. Stroma production of Cordyceps militaris (Clavicipitales: Clavicipitaceae) by injection of hyphal bodies to alternative host insects. Appl Entomol Zool 37:85-92.   DOI   ScienceOn
35 Stensrud O, Hywel-Jones NL, Schumacher T. 2005. Towards a phylogenetic classification of Cordyceps: ITS rDNA sequence data confirm divergent lineage and paraphyly. Mycol Res 109:41-56.   DOI   ScienceOn
36 Sung JM, Park YJ, Lee JO, Han SK, Lee WH, Choi SK, Shrestha B. 2006. Selection of superior strains of Cordyceps militaris with enhanced fruiting body productivity. Kor J Mycol 34:131-137.
37 Sung JM, Choi YS, Lee HK, Kim SH, Kim YO, Sung GH. 1999. Production of fruiting body using cultures of entomopathogenic fungal species. Kor J Mycol 27:15-19.
38 Sung GH, Hywel-Jones NL, Sung JM, Kuangsa-Ard JJ, Shrestha B, Spatafora JW. 2007. Phylogenetic classification of Cordyceps and the clavicipitaceous fungi. Stud Mycol 57:50-59.
39 Sung JM, Choi YS, Shrestha B, Park YJ. 2002. Investigation on artificial fruiting of Cordyceps militaris. Kor J Mycol 30:6-10.   DOI   ScienceOn
40 Wang L, Zhang WM, Hu B, Chen YQ, Qu LH. 2008. Genetic variation of Cordyceps militaris and its allies based on phylogenetic analysis of rDNA ITS sequence data. Fungal Diversity 31:147-155.
41 Wei HP, Xiao B, Hu KZ. 2004. Pharmaceutical values of Cordyceps militaris. J Chin Med Mater 27:215-217.
42 Wong YY, Moon A, Duffin R, Barthet-Barateig A, Meijer HA, Clemens MJ, De Moor CH. 2010. Cordycepin inhibits protein synthesis and cell adhesion through effects on signal transduction. J Biol Chem 285:2610-2621.   DOI   ScienceOn
43 Wu YH, Zhu S, Ding YH, Lou H. 1996. Artificial cultivation conditions of Cordyceps militaris and the analysis of its fruitbody compositions. Acta Edulis Fungi 3:61-63.
44 Wu FY, Yan H, Ma XA, Jia JQ, Zhang GZ, Guo XJ, Gui ZZ. 2011. Structural characterization and antioxidant activity of purified polysaccharide from cultured Cordyceps militaris. Afr J Microbiol Res 5:2743-2751.   DOI
45 Zhou XW, Gong ZH, Su Y, Lin J, Tang KX. 2009. Cordyceps fungi: natural products, pharmacological functions and developmental products. J Pharm Pharmacol 61:279-291.   DOI
46 Yoo HS, Shin JW, Cho JH, Son CG, Lee YW, Park SY, Cho CK. 2004. Effects of Cordyceps militaris extract on angiogenesis and tumor growth. Acta Pharm Sinica 25:657-665.
47 Yu HM, Wang BS, Huang SC, Duh PD. 2006. Comparison of preventive effects between cultured Cordyceps militaris and natural Cordyceps sinensis against oxidative damage. J Agaric Food Chem 54:3132-3138.   DOI   ScienceOn