• Korean Journal of Clinical Laboratory Science
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• v.50 no.4
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• pp.501-510
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• 2018

This study examined the neurotoxicity induced by lead acetate (LA) on cultured C6 glioma cells and the protective effects of Euphorbiae humifusae L. (EH) extract against LA-induced cytotoxicity. In this study, LA exhibited neurotoxicity in a dose-dependent manner compared to the control, and was determined to be highly-toxic according to the toxic criteria. The $XTT_{50}$ value of LA was calculated at a concentration of $38.6{\mu}M$ after C6 glioma cells were incubated for 72 hours in the media containing $30{\sim}50{\mu}M$ of LA, respectively. In addition, LA-induced neurotoxicity was suggested to correlate with the level of oxidative stress because vitamin E, an antioxidant, increased the cell viability damaged by LA-induced cytotoxicity. The EH extract effectively prevented cell injury from LA-induced cytotoxicity via its antioxidative effects, such as inhibitory ability of lipid peroxidation, superoxide dismutase-like activity and 1,1-diphenyl-2-picrylhydrazyl-radical scavenging activity. These antioxidative effects may result in components, such as polyphenol or flavonoids including gallic acid or quercetin. In conclusion, natural resources, such as EH extracts, may be a useful putative agent for the treatment of diseases associated with oxidative stress, such as lead neurotoxicity.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.3
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• pp.203-215
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• 2019

This study was conducted to identify the utilization of public health centers, as well as the individual characteristics and regional characteristics that affect their utilization based on data from the 2016 Community Health Survey, National Statistical Portal, and National Institute of Environmental Research. Independent samples t-tests, variance analysis, and multiple logistic regression analysis were used for analysis. Hierarchical multiple regression was used to analyze individual and regional characteristics. The results of hierarchical multiple regressions revealed that aged regions, women, older age individuals, respondents with lower education level and income level, walking practitioners, nutrition label readers, individuals experiencing depression, those who have received health checkups, those who are not covered by essential care, those who have spouses, and basic livelihood beneficiaries have increased use of public health centers. However, the use of public health centers decreased in stressors, and regions in which the population per 1,000, number of health care workers, health and welfare budget, fiscal independence, and unemployment rate were above the national average. As above, the central government and local governments need to analyze not only individual characteristics such as health behavior and psychological factors, but also regional characteristics, when establishing local health care policy.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.9
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• pp.495-503
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• 2019

The purpose of this study was to investigate the general characteristics, work overload, work life balance, job engagement of working adolescents and the factors affecting job engagement. We examined 1,772 working adolescents under the age of 24 years in response to the fifth Korean Working Conditions Survey. Technical statistics, t-test, ANOVA, and regression analysis were used. The study results showed that men had high-level job engagement when they were professional, self-employed, 41-52 hours per week, and never experienced employment discrimination. For women, job engagement was high if they were under 19 years of age, college graduates, professions, temporary work, 41-52 hours a week, and had never experienced gender discrimination, and employment discrimination. In addition, the job engagement of both men and women was higher when work overload was less and work life balance was good. This study is meaningful in that it examined the job engagement of working youth by using the Korean Working Conditions Survey representative of Korean workers. However, it has limitations in that it does not take into account specific circumstances such as academic status or military service. Based on the study results, it can be used as basic data for developing educational programs to manage job engagement and understanding of working adolescents.

• Journal of Convergence for Information Technology
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• v.9 no.9
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• pp.229-236
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• 2019

This study aims to examine the moderating effect of Extended Song Sharing for helping alcoholics to deal with stress. ESS is structure with non-verbal techniques approaches traditional Song Sharing technique. For this study, 64 alcoholics hospitalized were experimental (n=33) and control groups (n=31). The group was asked to participate in 12 ESS programs twice a week, every 45 minutes for six weeks. During this period, all those who belonged to the experimental and control groups were required to continue to participate in treatment programs prescribed by hospitals. Result: ESS interventional effects on subjects were evaluated using a stress response scale. As a result of the experiment, the WCC score of the experimental group increased 7.3% (p=0.000) but there was almost no change in the control group (1.5% increase, p=0.019). Effects of WCC types were higher in the experimental group, followed by 13.67% (p=0.006) in coping with wishful thinking, 9.66% (p=0.000) in the pursuit of social support, 6.35% (p=0.000) in dealing with problems, and 4% (p=0.000) in coping with emotions. ESS activities have been confirmed to improve the way alcoholics cope with stress.

• Journal of Life Science
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• v.29 no.1
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• pp.105-111
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• 2019

In recent years, progress has been made in the search for the development of new anti-cancer agents by employing specific inhibitors of histone deacetylase (HDAC)-6 to block signal transduction pathways in cancer cells. This study examined the effects of tubastatin A (TubA), an HDAC-6 inhibitor, on the growth and development of immature oocytes in murine ovaries using RNA sequencing analysis. The results from a gene set enrichment analysis (GSEA) indicated that the expression of most of the gene sets involved in the cell cycle and control and progression of meiosis decreased in the TubA-treated group as compared with that in germinal vesicle (GV) stage oocytes. In addition, an ingenuity pathway analysis (IPA) suggested that TubA not only caused increased expression of p53 and pRB and decreased expression of CDK4/6 and cyclin D but also caused elevated expression of genes involved in the control of the DNA check point in G2/M stage oocytes. These results suggest that TubA may induce cell cycle arrest and apoptosis through the induction of changes in the expression of genes involved in signal transduction pathways associated with DNA damage and the cell cycle of immature oocytes in the ovary.

• Korean Journal of Clinical Laboratory Science
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• v.51 no.2
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• pp.205-213
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• 2019

The protective effect of Agrimonia pilosa var. (AP) extract on potassium dichromate ($K_2Cr_2O_7$)-induced cytotoxicity in cultured NIH3T3 fibroblasts, was examined by performing an XTT assay for the cell viability and antioxidative effects, such as lactate dehydrogenase (LDH) activity and superoxide anion-radical (SAR) scavenging activity. In this study, $K_2Cr_2O_7$ decreased the cell viability significantly in a dose-dependent manner, and the $XTT_{50}$ value was determined to be $37.5{\mu}M$, which was highly-toxic according to the Borenfreund and Puerner' toxic criteria. The antioxidant, butylated hydroxytoluene (BHT), increased remarkably the cell viability damaged by $K_2Cr_2O_7$-induced cytotoxicity in these cultures. With regard to the protective effect of the AP extract on $K_2Cr_2O_7$-induced cytotoxicity, AP extract produced a significant increase in cell viability and antioxidative effects as the inhibitory ability LDH and SAR scavenging ability. These findings suggest that oxidative stress is involved in the cytotoxicity of $K_2Cr_2O_7$, and the AP extract effectively protected the cells from $K_2Cr_2O_7$-induced cytotoxicity by antioxidative effects. These results suggest that natural resources, such as AP extract, may be a putative therapeutic agent for the diminution or treatment of cytotoxicity induced by heavy metallic bases, such as $K_2Cr_2O_7$ correlated with oxidative stress.

• The Journal of the Korea Contents Association
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• v.20 no.12
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• pp.346-356
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• 2020

Purpose: This study was to identify the Health beliefs of the vulnerable group on the prevention of STI and to identify the factors that influence prevention behavior. Methods: The participants were 241 of persons vulnerable to STI. A total of 241 questionnaires were collected by mail or in person through relevant workplace, group members, and visitors to public health centers with structured questionnaires. The SPSS 21.0 program was used to perform descriptive statistics, t-test, one way ANOVA, Pearson's correlation and multiple regression. Results: As a result, the level of perceived susceptibility was 2.70, perceived severity was 3.63, perceived benefits of condom use was 3.82, Perceived barriers of condom use was 2.45, self-efficacy of condom use 3.93 and prevention behavior of STI was 3.51 based on 5-point Likert scale. Condom use self-efficacy(��=.23, p=.003), perceived barriers of condom use(��=-.15, p=.035), and level of education(��=.16, p=.018) were important factors influencing STI prevention behavior and the regression model explained 17% of STI prevention. In conclusion, it is necessary to develop a systematic education program in consideration of factors influencing and improving awareness to prevent STI.

• Journal of Korea Entertainment Industry Association
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• v.14 no.6
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• pp.289-299
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• 2020

The purpose of this study was to confirm the effect of mindfulness education on perceived stress, mindfulness, psychological well-being, and brain waves. The study used a repeated-measures design to examine 42 female university students currently enrolled in mindfulness courses. Subjects participated in the mindfulness course once a week for eight weeks, 50 minutes per session. The main outcome variables, such as perceived stress, mindfulness, and psychological well-being, were measured with a structured questionnaire and brain waves were measured with an electroencephalograph. The same variables were then measured after the completion of the eight-week course. The results demonstrate that perceived stress significantly decreased from 1.85±0.39 to 1.66±0.39 points (t=3.44, p=.001), while, on the other hand, mindfulness increased from 3.10±0.26 points to 3.19±0.32 points (t =-2.78, p=.008). There was also a significant difference in the level of autonomy, a subcategory of psychological well-being (t=-2.70, p=.010). In addition, EEG indicated significant changes in the frequencies of Theta channel 19 and Rapid Alpha channels 7 and 10. These results suggest that further studies are needed to elucidate the effects of mindfulness education through the modification of mindfulness course plans, diversification of teaching methods, and improvement in the educational environment.

• Journal of Korea Entertainment Industry Association
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• v.14 no.8
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• pp.203-211
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• 2020

This study is a similar experimental study using parallax design before and after the non-equality control group conducted to investigate the effects of the Mental health guardian program for mentally disabled people on the awareness of rights, empowerment, and resilience of mentally disabled people. The mentally handicapped persons who participated in the mental health guardian program were significantly higher in the awareness of the rights of the handicapped compared to the mentally handicapped persons who did not participate. In terms of empowerment, there was no significant difference between the two groups. In terms of resilience, the experimental group significantly increased compared to the control group. Among the sub-areas of resilience, excluding spirituality, difficulty overcoming, self-competence, learning and self-financing, basic functions, overall life satisfaction, new potential, advocacy and fullness all significantly increased. It has been shown to be effective in increasing the resilience of the disabled. In this study, the reason why mental health guardian program is effective for awareness of rights and resilience is not a one-way education, but a participatory curriculum in which a person with mental disabilities who participated in the education finds and corrects the wrong news and creates a card news again. It seems to be because it did. As a result of this study, it seems that the mental health guardian program has contributed to enhancing the recognition of the rights of the mentally impaired and enhancing resilience.

• Journal of Korea Entertainment Industry Association
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• v.15 no.8
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• pp.389-401
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• 2021

This study was performed to generate basic data to establish a health promotion plan for residents of Chungcheongbuk-do by identifying characteristics of discharged patients residing in the Chungcheongbuk-do area from an In-Depth Post-Discharge Injury Survey reported by the Korea Centers for Disease Control and Prevention(KCDCP). The Report provided data on demographic characteristics, medical institution use characteristics, medical use characteristics, and disease characteristics of patients discharged from medical institutions with 100 or more beds from 2013 to 2017. The total number of Chungcheongbuk-do residents who were admitted and discharged from 2013 to 2017 was estimated to be 1,656,590, and the discharge rate was 21,089, which was higher than the national average of 13,882 in 2016. The regions where the discharge rate increased during this period include Goesan, Yeongdong, Boeun, Okcheon, Jeungpyeong, and Eumseong-gun, which are mainly rural areas. Among the patients hospitalized and discharged from hospitals outside the Chungcheongbuk-do area, the discharge rate of patients who used hospitals in Incheon/Gyeonggi areas and Daejeon/Chungnam areas increased slightly. Among the malignant tumor patients, the number of lung cancer(included trachea & bronchial cancer) patients was the highest. In addition, the discharge rate was highest for patients with respiratory diseases. This study suggests that efforts need to be made to lower the discharge rate for infection, circulatory disease, genitourinary disease, and musculoskeletal disorder patients