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http://dx.doi.org/10.15324/kjcls.2018.50.4.501

Alleviating Effects of Euphorbiae humifusae L. Extract on the Neurotoxicity Induced by Lead  

Lee, Sang-Hee (Department of Internal Medicine, Sanbon Hospital, School of Medicine, Wonkwang University)
Seo, Young-Mi (Department of Nursing, College of Medicine, Wonkwang Health Science University)
Publication Information
Korean Journal of Clinical Laboratory Science / v.50, no.4, 2018 , pp. 501-510 More about this Journal
Abstract
This study examined the neurotoxicity induced by lead acetate (LA) on cultured C6 glioma cells and the protective effects of Euphorbiae humifusae L. (EH) extract against LA-induced cytotoxicity. In this study, LA exhibited neurotoxicity in a dose-dependent manner compared to the control, and was determined to be highly-toxic according to the toxic criteria. The $XTT_{50}$ value of LA was calculated at a concentration of $38.6{\mu}M$ after C6 glioma cells were incubated for 72 hours in the media containing $30{\sim}50{\mu}M$ of LA, respectively. In addition, LA-induced neurotoxicity was suggested to correlate with the level of oxidative stress because vitamin E, an antioxidant, increased the cell viability damaged by LA-induced cytotoxicity. The EH extract effectively prevented cell injury from LA-induced cytotoxicity via its antioxidative effects, such as inhibitory ability of lipid peroxidation, superoxide dismutase-like activity and 1,1-diphenyl-2-picrylhydrazyl-radical scavenging activity. These antioxidative effects may result in components, such as polyphenol or flavonoids including gallic acid or quercetin. In conclusion, natural resources, such as EH extracts, may be a useful putative agent for the treatment of diseases associated with oxidative stress, such as lead neurotoxicity.
Keywords
Antioxidative effect; Neurotoxicity; Oxidative stress;
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