• Journal of Physiology & Pathology in Korean Medicine
• /
• v.27 no.6
• /
• pp.782-788
• /
• 2013

Chrysanthemum zawadskii Herbich var. latilobum Kitamura (Compositae), colloquially known "Gujulcho" in Korea, has been used in traditional medicine for the treatment of various diseases, including cough, common cold, bladder-related disorders, gastroenteric disorders, hypertension, and inflammatory diseases, such as pneumonia, bronchitis, pharyngitis, and rheumatoid arthritis (RA) However, the effect of Chrysanthemum zawadskii var. latilobum on breast cancer invasion is unknown. In this study, we investigated the inhibitory effect of Chrysanthemum zawadskii var. latilobum extract (CZE) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced matrix metalloproteinase-9 (MMP-9) expression and cell invasion, as well as the molecular mechanisms involved in MCF-7 cells. CZE were not cytotoxic up to 100 ${\mu}g/ml$ concentration in the MCF-7 cell line. CZE decreased MMP-9 expression. TPA substantially increased NF-${\kappa}B$ DNA binding activity. Pre-treatment with CZE inhibited TPA-stimulated NF-${\kappa}B$ binding activity and NF-${\kappa}B$ related protein expression. To identify invasion ability of MCF-7 cells decreased by CZE, we used martrigel invasion assay. As a result, it is significantly decreased cell invasion. These results indicate that CZE-mediated inhibition of TPA-induced MMP-9 expression and cell invasion involves the suppression of the NF-${\kappa}B$ pathway in MCF-7 cells. Chrysanthemum zawadskii var. latilobum may have potential value in restricting breast cancer metastasis.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.39 no.4
• /
• pp.631-636
• /
• 2010

To develop a natural antioxidant from three Chrysanthemum species, flower and shoot extracts of Chrysanthemum frutescens, Chrysanthemum morifolium and Chrysanthemum zawadskii ssp. naktongense were obtained and their phenolic compound contents, scavenging effects on DPPH and ABTS radicals, ferrous ion chelating effects and inhibition activity on lipid peroxidation of linoleic acid were studied. Shoots of C. morifolium showed the highest levels in all above mentioned analyses. Especially, shoot extract of C. morifolium had high scavenging activities on ABTS radicals, similar to ascorbic acid or BHT. Ferrous ion chelating effect was the lowest in a C. morifolium shoot extract, but the highest in a C. morifolium flower extract. Inhibition activity on lipid peroxidation of linoleic acid was the highest with C. frutescens and C. morifolium shoots, but activity was lower than BHT. From present study, a shoot extract of C. morifolium is demonstrated as a valuable source for the development of a natural antioxidant. However, due to its low levels of ferrous ion chelating effects and inhibition activity on lipid peroxidation, a combination of other antioxidants with C. morifolium extract is recommended for the development of a new antioxidant.

• Korean Journal of Weed Science
• /
• v.14 no.1
• /
• pp.16-22
• /
• 1994

Ninty three species of domestic weeds were collected and screened for antimicrobial, antitumor, antioxidant and herbicidal activities. Among them, few showed antifungal activities. Cuscuta japonica showed inhibitory activity against Alternaria mali, Ambrosia artemisiifolia and Geranium sibiricum against Phytophthora capsici, Aster yomema and Aster pilosus against Phytophthora parasitica. Ambrosia artemisiifolia, Artemisia princeps, Artemisia capillaris, Ludwigia prostrata, Chrysanthemum zawadskii, Bidens frondosa, and Geranium sibiricum showed broad antibacterial activities. Carex chordorhiza, Artemisia capillaris, Persicaria nodosa, Senecio koreanus, Pariicum bisulcatum, Geranium sibiricum showed antiblebbing activity on human chronic leukemia K562 cell, among them, Persicaria nodosa was the strongist. Angelica decursiva, Equisetum arvense, Cimicifuga heracleifolia, Persicaria nodosa, Geranium sibiricum, Oenothera odorata, Cyperus sanguinolentus showed antioxidant activities. Ludwigia prostrata and Peucedanum terebinthaceum showed strong herbicidal activities.

• Food Science and Preservation
• /
• v.21 no.4
• /
• pp.529-535
• /
• 2014

To examine the levels of preservatives that occur naturally in food, wild plants used as commercial teas, rice cakes, or spices were studied according to the method of the Korean Food Code and analyzed with a gas chromatograph and HPLC. The levels of the natural preservatives (sodium dehydroacetate, sorbic acid, benzoic acid, methyl p-hydroxybenzoate, ethyl p-hydroxybenzoate, isopropyl p-hydroxybenzoate, propyl p-hydroxybenzoate, isobutyl p-hydroxybenzoate, butyl p-hydroxybenzoate, and propionic acid) in 21 cases were investigated against 15 kinds of wild plants. Six of 15 kinds of wild plants, such as pine needles, bamboo leaf, kudzu leaf, ramie leaf, mugwort, and nut pine leaf, were confirmed to have had sorbic acid, benzoic acid, and propionic acid. 8.201-21.839 mg/kg of benzoic acid was detected in the bamboo leaf, ramie leaf, pine needles, mugwort, kudzu leaf, and nut pine leaf. The sorbic acid contents of the bamboo leaf and the kudzu leaf were 5.630-24.995 mg/kg, respectively. The propionic acid content of the ramie leaf was 61.324-62.726 mg/kg. Nine kinds of wild plants, such as the Korean berchimia leaf, taro leaf, sasa borealis, lotus leaf, kuansh, chrysanthemum zawadskii, oak tree leaf, Chinese pepper leaf, and persimmon leaf, were not detected at the levels of the natural preservatives.

• Korean Journal of Agricultural Science
• /
• v.24 no.2
• /
• pp.267-274
• /
• 1997

To select potential inhibitors of platelet aggregation from large numbers of crude plant extracts, the modified thin smear method for the anti-platelet aggregating activity using platelet rich plasma was further modified by direct observation under a light microscope without staining the smear. The activities determined by the method were coincided with those by the electrical impedence method using whole blood, when ADP or collagen was employed as the aggregating agent. Among 130 varieties of edible and herbal plants which collected from markets or experimental farms of agricultural research institutes, those showed the anti-platelet aggregating activities were selected by testing the crude methanol extracts: Aster scaber, Aster tataricus, Ligularia stenocephala, Platycodon glaucum Allium victorialis, Allium oderum, Moros bombycis, Portulaco oleracea, Aamthopanax sessiliflorus and Rosa davurica. However, some of them activated the platelet aggregation under the same assay conditions: Pimpinella brachycarpa, Hosta plantaginea, Capsella bursapastoris, Fagopyrm esculentum, Prunus mume, Rubus coreanus and Limaria japonica. In addition, those revealed the antioxidant activities were selected by measuring the abilities to scavenge superoxide anion radicals: Pteridium aquilinum, Aster scaber, Ligularia fischeri, Chrysanthemum zawadskii, Artemisia capipparis, Cirsium setidens, Commelina communis and Capsella bursapastoris among edible plants.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2019.05a
• /
• pp.203-203
• /
• 2019

도시화로 인한 불투수면의 증가는 물순환 왜곡, 비점오염원 발생 및 수생태계 건상성 훼손 등을 야기시키며, 이를 해결하기 위하여 다양한 LID 기법을 적용하고 있다. 일반적으로 LID 내 적용 되는 여재들은 무기성여재로 중량이 크고 미세공극의 부재로 물리화학적 및 생물학적 저감기능이 제한적이다. 따라서 본 연구에서는 중량성이 낮은 생물폐자원을 선정하여 LID 시설 적용성평가를 수행하고자 한다. 생물폐자원은 발생량, 경량성 및 용이성을 고려하여 꼬막껍질(CS)과 호두껍질(WS)을 선정하였다. 생물폐자원의 산화부식을 고려하여 무기성 여재인 화산석과 혼합하여 Bioretention 시설에 적용하였으며, 여재 혼합비율에 따라 총 3가지의 Case 로 구성하였다. 식생은 구절초와 꽃댕강나무를 식재하였으며, 여재의 물리적 특성 분석을 위하여 적용 전과 후의 SEM(Scanning Electronic Microscope)을 수행하였다. 모니터링은 도로퇴적물 100g과 물 110L를 제조하여 인공강우유출수를 이용하여 수행하였으며, $0.0003{\sim}0.007m^3/sec$의 유속으로 주입하였다. 시설의 유입 및 유출부에서 유량 측정 및 수질 시료를 채취하였으며, 채취된 시료는 수질오염공정 시험법에 준하여 입자상 물질, 유기물, 영양물질 및 중금속 등을 분석하였다. Bioretention 시설의 모니터링 결과를 이용하여 물수지 및 TSS 저감 효율을 산정하였으며. 물수지 분석결과 시설의 저류율은 Case 1(soil) > Case 3(WS+RV) > Case 2(CS+RV) 순으로 나타났다. 시설 내 공극률이 가장 낮았던 Case 1에서 저류율이 약 55%로 가장 높게 것으로 분석되었다. Case 3(WS+RV)은 Case 2(WS+RV)와 시설 내 공극률이 유사함에도 불구하고 저류율이 약 10% 높은 것으로 분석되었다. 오염물질 저감효율 분석 결과, TSS와 TP의 제거효율은 모든 Case에서 약 75% 이상으로 높게 나타났으며, COD의 경우 모래를 적용한 Case 1에 비해 생물폐자원인 꼬막껍질과 호두껍질을 적용한 Case에서 약 1.3배 이상 높게 나타났다. 호두껍질과 꼬막껍질의 SEM 분석 결과 표면에 다공성이 형성되어 있는 것으로 조사되었다. 이는 여과 및 저류기작으로 인한 물순환 효과증대와 다공성과 돌기사이로 인한 입자상의 물질 여과 및 흡착으로 인하여 오염물질의 제거효율이 증대 된 것으로 평가된다. LID시설 내 생물폐자원과 무기성여재를 적절히 배합하여 복합여재로 조성할 경우 침하현상을 방지할 뿐만 아니라 저류 및 침투기능 향상과 미생물의 서식환경을 제공하기에 물순환 회복 및 비점오염물질 저감에 기여할 것으로 평가된다.

• Korean Journal of Plant Resources
• /
• v.32 no.1
• /
• pp.1-8
• /
• 2019

This study was performed to select the effective plant for phytoremediation of heavy metal contaminated areas. After cultivation of fifteen Compositae plants on soil contaminated with heavy metals for 8 weeks, the growth response and accumulation ability of each parts for heavy metal, such as arsenic, cadmium, copper, lead, and zinc were analyzed. Except Adiantum capillus-veneris, growth of Aster incisus, Coreopsis drumondii), Dendranthema indicum, Saussurea pulchella were relatively fine. Arsenic accumulation ability was the highest by Artemisia gmelini ($25.52mg{\cdot}kg^{-1}$ DW) in underground part, and D. sichotense ($3.35mg{\cdot}kg^{-1}$) in aerial part. Cadmium was the highest by Aster magnus ($2.50mg{\cdot}kg^{-1}$) in aerial part. Aerial and underground part of S. pulchella showed the highest copper accumulation (24.29, $99.92mg{\cdot}kg^{-1}$). In lead, 1.43 (A. magnus)${\sim}5.00mg{\cdot}kg^{-1}$ (S. deltoides) were accumulated in aerial part among fifteen Compositae plants. Aster hayatae ($140.09mg{\cdot}kg^{-1}$), Aster yomena ($109.07mg{\cdot}kg^{-1}$), A. magnus ($100.21mg{\cdot}kg^{-1}$) are absorbed more than $100mg{\cdot}kg^{-1}$ of Zinc. Therefore, they are considered to be phytoremediation material of zinc contaminated areas.

• Korean Journal of Pharmacognosy
• /
• v.49 no.4
• /
• pp.312-321
• /
• 2018

Recently, consumer demand for functional cosmetics containing natural ingredients has been greatly expanded. To develop the natural cosmetic materials, we selected 3 plants, Chrysanthemum zawadskii Herbich (CZ), Perilla frutescens (L.) Britton var. acuta Kudo (PF), and Rosa multiflora Thunberg (RM) which showed high total flavonoid contents (TFC), total polyphenol contents (TPC), and strong DPPH radical scavenging effect. We determined astragalin, chlorogenic acid, and rosmarinic acid as a marker compound for quantitative analysis of the content of each material and standardization of the quality standards and manufacturing standards through LC/MS analysis. HPLC-DAD was used to simultaneously analyze these marker components of three natural product complexes (Mix) and to validate the analytical method through experiments such as linearity, accuracy and precision. The detection wavelengths were set at 210, 265, and 330 nm. The detected 3 compounds from extract of CZ, PF, RM showed significant linearity ($R^2${\geq_-}$0.9947). The limit of detection (LOD) of chlorogenic acid, astragalin and rosmarinic acid were $8.29{\mu}g/ml$, $2.28{\mu}g/ml$, and $27.00{\mu}g/ml$, respectively. The limit of quantification (LOQ) of chlorogenic acid, astragalin and rosmarinic acid were $25.11{\mu}g/ml$, $6.92{\mu}g/ml$, and $81.83{\mu}g/ml$, respectively. The contents of the three indicators of Mix were 19.82-24.71 mg/g of chlorogenic acid, 43.80-46.02 mg/g of astragalin, and 46.33-48.57 mg/g of rosmarinic acid.

• Journal of agriculture & life science
• /
• v.50 no.5
• /
• pp.51-60
• /
• 2016

This study was selected drought tolerant plants, by observing the physiological characteristics and biochemical materials from the 9 kinds of the Compositae plants. After selecting plants of the similar size, and then drought stress was induced by the irrigation stopping. Survival rates, chlorophyll values, relative water content(RWC), excised-leaf water loss(ELWL), proline, reducing sugar were measured after 30 days of stopping irrigation. The species that had high rates of survival were Ainsliaea acerifolia Sch. Bip, Aster koraiensis, Aster scaber, Dendranthema zawadskii(S), however other 5 species were dead. The remaining factors have been determined based on plant species showed a higher survival rate. However, chlorophyll content showed high values in A. acerifolia, A.altaicus var. uchiyamae, A. koraiensis, and will have been determined that has no correlation with survival rates, except for A. acerifolia and A. koraiensis. On the other hand, A. scaber, A. acerifolia, A. koraiensis were determined to be relatively high drought tolerant plants in RWC, ELWL, proline, reducing sugar, it showed a similar correlation with survival rate. As a result of 9 kinds of the Compositae plants A. scaber, A. acerifolia, A. koraiensis were considered relatively higher drought tolerant plants.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.7 no.6
• /
• pp.75-83
• /
• 2004

Focusing on native plants that have high possibility of being introduced as rooftop material, this study was conducted to investigate extensive and easy-to-manage rooftop garden and to raise the utilization of native plants by verifying their growing response to artificial substrate soil depth and irrigation period. The study was conducted from March to September in 2002. Plants tested included Chrysanthemum zawadskii, Sedium middendorffianum, Thymus quinquecostatus, Allium senescens, and Dianthus superbus. Regarding soil depth, it was 5 cm and 10 cm. Irrigation period was non-irrigation, 1-week, 2-weeks, and 3- weeks, Its result is as follows; 1. In case of Sedum middendorffianum Maxim, mortality rate was 0% regardless of soil depth and irrigation period making it very suitable material for rooftop garden. 2. In case of Allium senescens L., mortality rate was 0% regardless of soil depth and irrigation period making it very suitable material for rooftop garden. Therefore, Provided that fertilizing is managed well, it is a plant that can be highly utilized.3. In case of Chrysanthemum zawadskii Herb. Subsp. (Nakai) Y. Lee Stat., the growth of top was lower in 10cm than in 5cm and it grew well in 10cm. When utilizing for rooftop garden, it would be desirable to keep minimum viable soil depth at over 10cm. If there is enough rainfall, soil and soil depth seem to have greater effect on growth than irrigation period does. 4. In case of Diauthus superbus L. var. longicalycinus (Maxim) Williams, rooting rate and growth were better in 10cm than in 5cm. Therefore, it is desirable to keep minimum soil depth at over 10cm. 5. In case of Thymus quinquecostatus Celak, the growth of top and flowering were better in 10cm than in 5cm. Therefore, it seems desirable to have minimum viable soil depth to be over 10cm. In conclusion, the most suitable species for rooftop garden are Sedium middendorffianum and Allium senescens in this experiment. However, Chrysanthemum zwadskii, Thymus quinquecostatus, and Dianthus chinensis also can be utilized greatly when irrigation is managed regularly in artificial mixed soil over 10cm.