• Journal of Food Hygiene and Safety
• /
• v.31 no.1
• /
• pp.21-27
• /
• 2016

The objectives of this study are to produce monoclonal antibodies (MAbs) against Vibrio parahaemolyticus and to develop an immuno-selective filtration (ISF) method for the rapid and sensitive detection of V. parahaemolyticus. The characterization of the MAb produced from HKVP 4H9-9 hybridoma cell was validated by enzyme-linked immunosorbent assay (ELISA) and western blot. The produced MAb was specific to V. parahaemolyticus and showed weak cross-reaction to V. alginolyticus, V. vulnificus and Staphylococcus aureus. After optimization of the method, $5{\times}10^1cell/mL$ of V. parahaemolyticus in a pure culture could be detectable. Although weak cross-reactivity to V. vulnificus, V. alginolyticus and Staphylococcus aureus was observed, the ISF was confirmed to be highly specific to V. parahaemolyticus. Especially, the ISF showed the most sensitivity compared to the immunoassays currently reported is easier to perform and quicker than ID-ELISA.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.15 no.9
• /
• pp.5419-5424
• /
• 2014

A weld scallop is a small quadrant or half circle type hole installed in the weld line cross area for easy welding operation. Many types of T-bars with a scallop can be welded in a block assembly stage in shipbuilding. The difficulties arise from the fact that scallops are to be filled by build-up welding after welding of the cross line is complete. In this study, a non-scallop block joint welding method was developed using special type CBM (ceramic backing material). The wedge shaped CBM was devised to insert a CBM into just the V groove of weld line cross area without weld scallop. A saw-toothed shape was adopted for easy cutting of the unnecessary part in the CBM fitting process. The applicability of the developed method was verified through welding experiments based on the yard welding conditions.

• The Journal of the Convergence on Culture Technology
• /
• v.8 no.6
• /
• pp.733-739
• /
• 2022

A survey was conducted on safety and health managers to analyze what change have occurred in the safety health management system of companies since the enforcement of the Serious Accidents Punishment Act. As a result, it was confirmed that most of the safety and health budgets increased, and most of the safety and health organization operations and safety and health tasks were strengthened. However, further research is needed to see if this has a practical effect on worker safety and reduction of serious accidents, and realistic measures are needed to allow safety and health managers to focus on practical helpful tasks rather than formal ones. In addition, it was confirmed that active support from business owners or management managers and strengthening education on the Serious Accidents Punishment Act were needed through cross-analysis between the education of the Serious Accidents Punishment Act and positive change in safety and health.

• Tuberculosis and Respiratory Diseases
• /
• v.60 no.2
• /
• pp.171-179
• /
• 2006

Background : Despite the emerging danger of MDR-TB to human beings, there have only been a limited number of drugs developed to treat MDR-TB since 1970. This study investigated the cross-resistance rate between rifampicin (RFP) and rifabutin (RBU) in order to determine the efficacy of rifabutin in treating MDR-TB. In addition, the results of rifabutin were correlated with the rpoB mutations, which are believed to be markers for MDR-TB and RFP resistance. Methods : The MICs of RBU were tested against 126 clinical isolates of MDR-TB submitted to the clinical laboratory of National Masan TB Hospital in 2004. Five different concentrations ($10-160{\mu}g/ml$) were used for the MICs. The detection of the rpoB mutations was performed using a RFP resistance detection kit with a line probe assay(LiPA), which contains the oligonucleotide probes for 5 wide type and 3 specific mutations (513CCA, 516GTC, and 531TTG) The rpoB mutation was determined by direct sequencing. Results : The rate of cross-resistance between RFP and RBU was 70.5%(74/105) at $20{\mu}g/ml$ RBU(ed note: How much RFP?) Most mutations (86.3%) occurred in the 524~534 codons. The His526Gln, His526Leu, Leu533Pro, Gln513Glu, and Leu511Pro mutations(Ed note: Is this correct?) were associated with the susceptibilty to RBU. Conclusion : Based on the cross-resistance rate between RFP and RBU, RBU may be used effectively in some MDR-TB patients. Therefore, a conventional drug susceptibility test for RBU and a determination of the critical concentration are needed. However, rpoB gene mutation test may be have limited clinical applications in detecting RBU resistance.

• Parasites, Hosts and Diseases
• /
• v.31 no.2
• /
• pp.141-148
• /
• 1993

ELISA-inhibition test using Paragonimus westermani specific monoclonal antibody (Mab) was investigated to improve the diagnostic specificity of paragonimiasis. By cell fusion, one hybridoma clone secreting un-n westemanl specific Mab was selected (Pwa-14), which reacted on bands of 28 kDa, 42.5 kDa, 89 kDa and 120.5 kDa. IFA showed Pwa-14 was located at the vitelline follicles. By micro-ELISA, 100% of 22 paragonimiasis cases were found positive, but 5 of 40 clonorchlasls cases (12.5%),3 of 26 cystlcercosis cases (7.7%) showed false positive. None of 10 sparganosis patients or 28 normal controls reacted positively. On the other hand, by ELISA-Inhibition test using a R westermcni specific Mab, 100% of patagonimlasls cases were found positive, and there were no positive in cysticercosis, sparganosis cases or normal controls, except 2 (5.0%) false-positive sera of 40 clonorchiasis cases. The ELISA-Inhlbltlon test using a Mab showed higher specificity in comparison with macro-ELISA for serodlgnosis of human paragonimlasis.

• Journal of Food Hygiene and Safety
• /
• v.34 no.4
• /
• pp.334-339
• /
• 2019

A procedure based on high-performance liquid chromatography (HPLC) is described to determine ${\beta}-carotene$ in infant formulas. The method for ${\beta}-carotene$ analysis was performed on a C18 reversed-phase column using acetonitrile/methanol/dichloromethane (6:1:3, v/v/v) as a mobile phase. ${\beta}-Carotene$ was determined in HPLC with photo diode array (PDA) detector. The parameters of validation were specificity, linearity, LOD, LOQ, accuracy, precision and repeatability. The specificity was confirmed by the retention time and the linearity ($R^2$), which was over 0.999 in the range of 0.125~2 mg/L. The detection and quantification limits were 0.064 and 0.193 mg/L, respectively. The accuracy and precision of this method using an STD spiked sample were 80~119% and 1.02~2.05% respectively. The method was applied to the analysis of various infant formula and follow-up formulas products containing ${\beta}-carotene$, and all the products contained acceptable levels of ${\beta}-carotene$ for nutrition labeling.

• Journal of Veterinary Clinics
• /
• v.16 no.1
• /
• pp.80-85
• /
• 1999

Malassezia(Pityrosoprum) pachydermatis에 의한 피부염의 위험요인을 파악하고자 1997년 1월에서 1998년 12월까지 서울대학교 수의과대학 부속동물병원에 내원하여 본 질환으로 진단된 29두와 동일한 기간 동안 피부과 검사에서 임상적으로 건강한 개로 확인된 97 두의 대조군을 대상으로 분석하였다. 각 설명변수에 대한 교차비(odds ratio, OR)와 신뢰구간(confidence interval, CI)은 변수보정법을 사용하여 계산하였다. 다른 공변수를 보정한 상태에서 다중 로지스틱 회귀분석(multiple logistic regression analysis)을 시행한 결과 피부병기왕력(p=0.0059; OR, 4.44; CI, 1.5∼12.8)과 비만(p=0.0013; OR, 5.38; CI, 1.9∼14.9)은 유의한 위험요인으로 나타났다. 단변량 분석(univariate analysis)에서 주 사료로 고기를 섭취하는 경우 피부염의 위험이 높은 변수로 작용하였지만(p=0.0398; OR, 6.38; CI, 1.1∼37.3) 다른 공변수를 보정한 분석에서는 유의하지 않은 것으로 나타났다. 성별, 품종, 및 예방접종은 위험요인으로 작용하지 않았다.

• Proceedings of the KIPE Conference
• /
• 2017.11a
• /
• pp.71-72
• /
• 2017

본 논문에서는 비선형 최적화 기법을 이용하여 자기 포화(magnetic saturation) 및 교차 결합 현상(cross-coupling effect)을 고려한 매입형 영구자석 전동기(IPMSM)의 실시간 MTPA 제어 방법을 제안한다. 이는 토크 지령 추종과 최소 동손 운전을 만족하는 제한 최적화(constraint optimization) 문제로 접근할 수 있다. 이를 통해 유도한 연립 비선형 방정식의 경우, Levenberg-Marquardt 수치 해석법을 적용하여 안정적이면서 빠르게 해를 구할 수 있다. 이러한 방법을 이용하면 참조표(look-up table) 없이 운전 환경의 실시간 변동을 고려한 효율적인 MTPA 운전이 가능하다. 시뮬레이션을 통해 제안된 알고리즘의 전류 해가 최적 운전점과 일치함을 확인하였다.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
• /
• 2011.11a
• /
• pp.63-65
• /
• 2011

부산신항은 지형적인 특성상 마산 방면에서 입출항하는 선박과 신항으로 입출항하는 선박이 서로 교차하는 경우가 빈번하고 대형 컨테이너선과 저속 예부선 등의 통항량이 많을 뿐만 아니라 년 중 안개일수도 많아 충돌사고 위험이 상존한다. 따라서 선박에 정보제공 정도만 하는 소극적 관제 보다는 선박의 이동에 개입하는 적극적인 관제가 사고 방지에 더욱 효과적이라 할 수 있다. 부산신항의 관제 자료 및 해양안전심판원의 재결사례 등을 통하여 충돌사고의 원인을 분석하는 과정에서 적극적 관제의 필요성을 확인할 수 있었다. 본고에서는 부산신항에서의 적극적 관제 사례와 문제점을 주로 법률적인 측면에서 살펴보고, 적극적 관제의 개선 방안을 도출하여 보았다.

• Composites Research
• /
• v.30 no.6
• /
• pp.331-337
• /
• 2017

The composite lattice structures have advantages of high specific stiffness and strength and are mainly applied to the structures of launch vehicles that carry the compressive load. However, since these structures are manufactured by filament winding technology, there are some defects and voids found in the knots. For these reasons, the stiffness and strength of the lattice structures have to be compared with finite element model for predicting design load. But, the full scale test is difficult because time and space are limited and the shape of structure is complex, and hence the simple and reliable test methods for examination of stiffness are needed. In this paper, subelements of composite lattice structures were prepared and compressive and bending test were conducted for examination of stiffness of helical and hoop rib. Test methods for subelements of composite lattice structures that has curved and twisted shape were supposed and compared with finite element analysis results.