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http://dx.doi.org/10.13103/JFHS.2016.31.1.21

Production of Monoclonal Antibodies against Vibrio parahaemolyticus and Development of High Sensitive Immuno-Selective Filtration Method  

Kim, Jeong-Sook (Institute of Agriculture and Life Science, Gyeongsang National University)
Choi, Young-Dong (Division of Applied Life Science, Graduate School, Gyeongsang National University)
Shim, Won-Bo (Institute of Agriculture and Life Science, Gyeongsang National University)
Publication Information
Journal of Food Hygiene and Safety / v.31, no.1, 2016 , pp. 21-27 More about this Journal
Abstract
The objectives of this study are to produce monoclonal antibodies (MAbs) against Vibrio parahaemolyticus and to develop an immuno-selective filtration (ISF) method for the rapid and sensitive detection of V. parahaemolyticus. The characterization of the MAb produced from HKVP 4H9-9 hybridoma cell was validated by enzyme-linked immunosorbent assay (ELISA) and western blot. The produced MAb was specific to V. parahaemolyticus and showed weak cross-reaction to V. alginolyticus, V. vulnificus and Staphylococcus aureus. After optimization of the method, $5{\times}10^1cell/mL$ of V. parahaemolyticus in a pure culture could be detectable. Although weak cross-reactivity to V. vulnificus, V. alginolyticus and Staphylococcus aureus was observed, the ISF was confirmed to be highly specific to V. parahaemolyticus. Especially, the ISF showed the most sensitivity compared to the immunoassays currently reported is easier to perform and quicker than ID-ELISA.
Keywords
Vibrio parahaemolyticus; monoclonal antibody; ELISA; immuno-selective filtration;
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