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http://dx.doi.org/10.13103/JFHS.2019.34.4.334

Determination of β-Carotene in Infant Formulas by High-Performance Liquid Chromatography  

Hwang, Kyung Mi (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation)
Bae, Ji Won (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation)
Hu, Soo Jung (Nutrition and Functional Food Research Team, National Institute of Food and Drug Safety Evaluation)
Oh, Keum Soon (Food Additives Standard division, Ministry of Food and Drug Safety)
Publication Information
Journal of Food Hygiene and Safety / v.34, no.4, 2019 , pp. 334-339 More about this Journal
Abstract
A procedure based on high-performance liquid chromatography (HPLC) is described to determine ${\beta}-carotene$ in infant formulas. The method for ${\beta}-carotene$ analysis was performed on a C18 reversed-phase column using acetonitrile/methanol/dichloromethane (6:1:3, v/v/v) as a mobile phase. ${\beta}-Carotene$ was determined in HPLC with photo diode array (PDA) detector. The parameters of validation were specificity, linearity, LOD, LOQ, accuracy, precision and repeatability. The specificity was confirmed by the retention time and the linearity ($R^2$), which was over 0.999 in the range of 0.125~2 mg/L. The detection and quantification limits were 0.064 and 0.193 mg/L, respectively. The accuracy and precision of this method using an STD spiked sample were 80~119% and 1.02~2.05% respectively. The method was applied to the analysis of various infant formula and follow-up formulas products containing ${\beta}-carotene$, and all the products contained acceptable levels of ${\beta}-carotene$ for nutrition labeling.
Keywords
${\beta}-carotene$; HPLC; Infant formula;
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1 David, C.W., Anja, B., Harvey, I., Adrienne, M.: Determination of vitamin A and vitamin E esters in infant formulae and fortified milk powders by HPLC : Use of internal standardisation. Food Chem. Acta, 197, 457-465 (2016).   DOI
2 AOAC official method 2016.13., Official methods of analysis of AOAC INTERNATIONAL 20th Ed. AOAC International, Gaithersburg, MD (2016).
3 Standards for health functional food of Korea. Ministry of Food and Drug Safety, Cheongju, Korea (2018).
4 Rodeney, J.B. : Determination of $\alpha$-and $\beta$-carotene in some raw fruits and vegetables by high-performance liquid chromatography. J. Agric. Food Chem. 34(3), 409-412 (1986).   DOI
5 Official methods of analysis of AOAC INTERNATIONAL 19th Ed., Appendix L. AOAC INTERNATIONAL, Dr. George WL Jr, Recommended Guidelines for SPIFAN Single Laboratory Validation (SLV) (2012).
6 Holick, C.N., Michaud, D.S., Stolzenberg-Solomon, R., Mayne S.T., Pietinen, P., Taylor, P.R., Virtamo, J., Albanes, D.: Dietary carotenoids, serum $\beta$-carotene, aand retinol and risk of lung cancer in the alpha-tocopherol, beta-carotene cohort study. Am. J. Epidemiol. 156(6), 536-547 (2002).   DOI
7 Noel W.S.Olson J.A. 2012. Chapter 11 Vitamin A. Present knowledge in nutrition. Tenth ed. Wiley Press, Washington D.C.
8 Chytil, F.: Retinoids in lung development. FASEB J., 9, 986-992 (1996).   DOI
9 Thompkinson, D.K., Kharb, S.: Aspects of infant food formulation. Comprehensive Rev. Food Sci. Food Safety, 6, 79-102 (2007).   DOI
10 Ok, K.H., Kim, K.S., Seo, J.S., Choi, Y.S., Shin, S.M.: A study on the nutrient intakes and supplemental food of infants in relation to the method of feeding practice. Kor. J. Nutr. 29(2), 143-152 (1996).
11 CODEX ALIMENTARIUS: Standard for infant formula and formulas for special medical purposes intented for infants CODEX Stan 72-1981, FAO, WHO (2011).
12 Food Code of Korea. Ministry of Food and Drug Safety, Cheongju, Korea (2018).
13 AOAC official method 2005.07., Official methods of analysis of AOAC INTERNATIONAL 20th Ed. AOAC International, Gaithersburg, MD (2016).
14 Thomas, J.B., Kline, M.C., Gill, L.M. Yen, J.H., Duewer, D.L., Sniegoski, L.T., Sharpless, K.E.: Preparation and value assignment of standard reference material 968c fat-soluble vitamins, carotenoids, and cholesterol in human serum. Clin. Chim. Acta, 305, 141-155 (2001).   DOI